382 results on '"Franciolli, A."'
Search Results
352. Coagulation and fibrinolysis in acute mountain sickness and beginning pulmonary edema
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André Haeberli, P. W. Straub, E. K. Kruithof, M. Franciolli, and Peter Bärtsch
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Adult ,Male ,medicine.medical_specialty ,Epinephrine ,Physiology ,medicine.medical_treatment ,Pulmonary Edema ,Hematocrit ,Altitude Sickness ,Norepinephrine ,Reference Values ,Physiology (medical) ,Internal medicine ,Fibrinolysis ,medicine ,Decreased fibrinolysis ,Humans ,Hypoxia ,Blood Coagulation ,Blood coagulation test ,Low altitude ,Proteinuria ,medicine.diagnostic_test ,business.industry ,Blood Proteins ,Pulmonary edema ,medicine.disease ,Surgery ,Coagulation ,Cardiology ,Blood Coagulation Tests ,medicine.symptom ,business - Abstract
To examine whether intravascular coagulation and/or decreased fibrinolysis precedes high-altitude pulmonary edema (HAPE) we examined 25 male mountaineers (median age 40 yr) at low altitude (550 m) and after 6, 18, and 42 h at an altitude of 4,559 m, which was climbed in 24 h. In 14 subjects, 2 of whom showed radiological evidence of HAPE after 42 h, symptoms of acute mountain sickness (AMS) were mild or absent. Eleven subjects suffered from AMS, six of whom developed radiologically documented HAPE after 18 or 42 h. In the absence of AMS there were no significant changes at high altitude, with the exception of a decrease in bleeding time from 246 +/- 18 to 212 +/- 13 (SE) (P less than 0.05). In AMS, partial thromboplastine time decreased from 34.2 +/- 0.8 to 31.1 +/- 0.5 s (P less than 0.001) and factor VIII procoagulant activity and von Willebrand factor antigen were increased by 57 +/- 12 and 70 +/- 13%, respectively (P less than 0.001), whereas there were no significant changes in beta-thromboglobulin (BTG), fibrinopeptide A (FPA), and fibrin fragment B beta 15–42. In subjects with HAPE, BTG, FPA, and B beta 15–42 were normal before and in beginning HAPE. Preceding HAPE, euglobulin clot lysis time declined at high compared with low altitude from 289 +/- 48 to 201 +/- 42 min without venous occlusion (VO) and from 107 +/- 36 to 86 +/- 31 min after VO (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
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- 1989
353. Lo sfruttamento delle acque moesane da parte delle officine idroelettriche di Mesolcina S.A
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Franciolli, Edoardo
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- 1959
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354. Arnoldo Marcelliano Zendralli
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Franciolli, Edoardo
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- 1963
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355. Le figure femminili nell'Orlando Furioso
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Franciolli, Edoardo
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- 1959
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356. Per la scuola del Grigioni Italiano
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Franciolli, Edoardo
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- 1961
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357. Wirtschaftliche Probleme
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Franciolli, Edoardo
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- 1954
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358. La lettura nelle nostre scuole
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Franciolli, Edoardo
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- 1965
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359. Panoramica nazionale dello zinco tra il 2010 e il 2014
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Franciolli da Silva Dantas Araújo, Amanda Alves Fecury, Euzébio Oliveira, Carla Viana Dendasck, Claudio Alberto Gellis de Mattos Dias, Alex Costa Matos, Franciolli da Silva Dantas Araújo, Amanda Alves Fecury, Euzébio Oliveira, Carla Viana Dendasck, Claudio Alberto Gellis de Mattos Dias, and Alex Costa Matos
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Lo zinco è un elemento chimico di numero atomico 30, con massa atomica 65,4, trovato in natura con le maggiori riserve che si trovano in Australia e Cina, con il Brasile che ha una riserva quasi trascurabile. Lo zinco può anche essere trovato sotto forma di solfuri e ossidi, associati a piombo, rame, argento e ferro. La produzione brasiliana di zinco dal 2010 al 2014 è stata di 895 (10³t). Questo articolo mira a presentare la riserva mondiale di zinco tra il 2010 e il 2014, oltre alla produzione brasiliana, all’importazione, all’esportazione e ai consumi interni e al suo prezzo sul mercato tra il 2010 e il 2014. La ricerca è stata condotta accedendo al sito web del Dipartimento Nazionale di Produzione Mineraria – DNPM (http://www.dnpm.gov.br/). Nelle riserve mondiali di zinco, l’Australia aveva la media più alta, seguita da Cina, Perù e Messico, il Brasile aveva la media più bassa tra i paesi. Nella produzione brasiliana di zinco, abbiamo osservato una costante diminuzione in crescita nel 2014. L’altro anno le importazioni sono rimaste instabili e le esportazioni hanno avuto un forte calo. Il consumo di concentrato in Brasile diminuisce e a causa della crisi economica il consumo del metallo primario è rimasto instabile. Il prezzo dello zinco tra il 2010 e il 2014 è rimasto instabile a causa di fattori quali la produzione mondiale e il mercato finanziario mondiale.
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- 1970
360. Morfologia e funcionalidade do pneumócito tipo II e sua relação e variação com a idade gestacional em bovinos
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Toquetti, Rd, Guerra, Rr, Ambrosio, Ce, Santos, Jm, Favaron, Po, Franciolli, Alr, Alberto, Mv, Meirelles, Fv, and Miglino, Ma
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type II pneumocyte ,pulmão ,bovine ,proteína surfactante ,pneumócito tipo II ,surfactant protein ,bovino ,lung - Abstract
Este estudo objetivou caracterizar a presença de pneumócitos tipo II e o início da produção de lipoproteína surfactante em bovinos, correlacionando a idade gestacional com a síntese de surfactante durante o desenvolvimento fetal. Pulmões de fetos com quatro meses de idade gestacional estavam na fase canalicular de desenvolvimento, sem a presença de pneumócitos tipo II ou bandas eletroforéticas compatíveis com a presença de proteínas surfactante. No 5° mês gestacional, os pulmões dos fetos encontravam-se em fase de saculação terminal, com a presença de alvéolos por epitélio cúbico, com áreas formadas por pneumócitos I e II. Nesse período ainda não foi possível identificar proteína surfactante nos pulmões. Esses órgãos em fetos com seis meses de idade gestacional estavam em fase de saco terminal, com presença de pneumócitos tipo I e II. Nessa fase a análise para determinação protéica do surfactante de feto bovino (SDS - PAGE) demonstrou presença de bandas entre 26 e 36kDa, confirmando produção de SP - A, proteína surfactante encontrada em maior quantidade. A partir do 7° mês gestacional, a fase de saco terminal é mais evidente e complexa, com desenvolvimento de intensa vascularização. O pneumócito tipo I apresentava aspecto mais pavimentoso, e o tipo II apresentava aspecto mais globoso. Na análise SDS - PAGE do lavado bronco - alveolar, bandas de proteína surfactante com aspecto similar ao de animais recém-nascidos foram encontradas. Em recém-nascidos, pulmões na fase alveolar foram observados com pneumócitos tipo I e II característicos. O perfil das bandas do lavado bronco-alveolar dos recém-nascidos foi igual ao de animais adultos. Esses achados sugerem que um animal nascido precocemente, a partir dos sete meses de gestação, teria sua sobrevivência garantida devido a uma possível funcionalidade do sistema respiratório do feto, pois o pulmão possuiria as características necessárias para a síntese de proteínas surfactantes. Entretanto, mais estudos clínicos sobre a funcionalidade do sistema respiratório abrem novas fronteiras de experimentos sobre fisiologia respiratória em recém-nascidos bovinos. The aim of this study was to characterize the presence of type II pneumocyte and the beginning of the surfactant protein production in bovines to the correlated gestational age. Lungs of fetuses at 4 months gestational age were found to be in the canalicular development phase, without presence of type II pneumocytes or appearance of electrophoretic bands compatible with the presence of the surfactant protein. In fetuses at 5 months of gestational age, the lungs were at the terminal sac phase, with the presence of primitive alveolus, formed by cubical epithelium and areas formed by pneumocytes I and pneumocytes II. No electrophoretic bands compatible to the surfactants proteins were identified. In contrast, lungs of fetuses at 6 months of gestational age showed, development of terminal sac phase, with the presence of type I and type II pneumocytes. In this phase, proteic determination for the SDS - PAGE analysis showed the presence of bands between 26 to 36kDa, demonstrating SP - A production, which is the surfactant protein found in highest amount. From the 7th gestational month on, the phase of terminal sac is more evident and complex, with intense sprouting vascularization. The type I pneumocyte had a more squamous aspect and type II pneumocyte were more globular. In the SDS - PAGE analysis of the bronchial - alveolar wash, surfactant protein bands had been observed with a profile similar to that of the newborn animals. In newborn animals, lungs in alveolar phase showed the development of type I and II pneumocytes. The profile of the bronchial - alveolar wash from the newborn was similar to an adult animal, presenting the same bands in triplets. These results suggest that early born fetuses, from 7 months of pregnancy could have a guarantee of surviving due to possible functionality of respiratory system because the lung presented all necessary features for surphactant protein syntheses. However, new clinical studies related to respiratory system functionality open new venues for newborn bovine's experiments respiratory physiology.
361. Morphology and functionality of the type II pneumocytes and their variation in relation to bovine gestational age,Morfologia e funcionalidade do pneumócito tipo II e sua relação e variação com a idade gestacional em bovinos
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Toquetti, R. C., Ricardo Guerra, Ambrosio, C. E., Santos, J. M., Favaron, P. O., Franciolli, A. L. R., Alberto, M. V., Meirelles, F. V., and Miglino, M. A.
362. Morphofunctional study of crab-eating raccoon (Procyon cancrivorus) mammary gland,Estudo morfofuncional das glândulas mamárias de Mão Pelada, Procyon cancrivorus
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Bellatine, T., Mançanares, C. A., Franciolli, A. L. R., Carlos Eduardo Ambrósio, Martins, D. S., Miglino, M. A., and Carvalho, A. F.
363. Development of the main organs in buffalo embryos (Bubalus bubalis)
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Morini, A. C., Carlos Eduardo Ambrósio, Martins, D. S., Morini, J. C., Favaron, P. O., Franciolli, A. L. R., Pereira, F. V. T., Minervino, A. H. H., and Miglino, M. A.
364. Morphology of the main vessels of the coati brain (Nasua nasua)
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Barreiro, Juliana Regina, Carvalho, Ana Flavia, Franciolli, Andre Luis R., Ferreira, Guilherme Jose B. C., Ferreira, Jussara R., Carlos Eduardo Ambrósio, and Miglino, Maria A.
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quati ,coati ,encephalic vascularization ,Nasua nasua ,vascularização encefálica - Abstract
Estudou-se a morfologia do encéfalo de Nasua nasua - quati, buscando comparar estes achados com outras espécies já descritos. Foram utilizados cinco encéfalos de quatis, provenientes do Criatório Científico (Cecrimpas), Unifeob. Os animais foram eutanásiados de acordo com a legislação (Cobea). Canulou-se a artéria carótida comum e a veia jugular externa sentido cranial, injetou-se solução de látex/bário corado de vermelho na artéria carótida, e solução de látex corado de azul na veia jugular. Em seguida os animais fixados em solução de formaldeído a 10%. O encéfalo tem sua nutrição dependente de quatro artérias, as artérias carótidas internas e as artérias vertebrais direitas e esquerdas. Esses vasos compuseram o circuito basilar e carotídeo que se anastomosam através das artérias cerebrais caudais. Correm na base do encéfalo sob a meninge pia mater. The morphology of the brain of coati, Nasua nasua, was studied, to compare the findings with other species described. The brains of five coatis were used, proceeding from the Scientific Breeding School (Cecrimpas), Unifeob. The animals were sacrificed in accordance with the legislation (Cobea). With a needle, the common carotid artery and the external jugular vein was cannulated to cranial direction, injected latex solution stained with colored red barium respectively. Afterwards the animals were fixed in 10% formaldehyde solution. Brain has its dependent nutrition of four arteries, the internal carotid arteries and the right and left vertebral arteries. These vessels had composed the basal and carotid circuits that anastomose through the cerebral arteries volumes. They run in the base of the brain under piamater meninges.
365. Morphological study of raccon male genital organs (Procyon cancrivorus)
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Martucci, Mariane Ferracin, Mancanares, Celina Almeida, Carlos Eduardo Ambrósio, Rezende Franciolli, Andre Luis, Miglino, Maria Angelica, Rosa, Ricardo Alexandre, and Carvalho, Ana Flavia
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carnívoros ,Morphology ,morfologia ,Procionídeos ,Carnivores ,Animais silvestres ,Procyonidae ,Wild animals - Abstract
Foram utilizados três animais, sendo um proveniente da Universidade de São Paulo e dois encaminhados pelo IBAMA (Proc. 02027.000.286/2004-92), excedente populacional de zoológico. Os aparelhos reprodutores masculinos "ex situ" foram fixados por imersão e dissecados. Foram retirados fragmentos de cada órgão e glândula anexa do aparelho reprodutor, os quais foram processados e incluídos pelas técnicas de inclusão em paraplast. Cada bloco foi cortado e os cortes foram corados por HE, picrosírius, reação de PAS com fundo de hematoxilina e tricromo de masson para a observação das estruturas ao microscópio óptico. Macroscopicamente, a região inguinal era composta pela uretra, músculo isquiocavernoso, músculos bulboesponjoso e bulbo cavernoso, músculo retrator do pênis, um par de testículos e o osso peniano ou báculo e ânus. A glande do pênis apresentou uma dilatação proximal (bulbo da glande) constituída pela parte dilatada do báculo. A posição dos testículos, dentro do escroto, era horizontal. A próstata apresentou-se com formato globoso, circundando a uretra. Microscopicamente, os testículos eram envoltos por uma cápsula de tecido conjuntivo denso, a túnica albugínea testicular. O ducto epididimário era provido de um epitélio pseudoestratificado com estereocílios. A uretra peniana apresentou-se circundada pelo corpo esponjoso e no restante do pênis apresentou o corpo cavernoso (tecido erétil). Os resultados macro e microscópicos encontrados até o momento são semelhantes aos achados no Canis familiaris (cão doméstico). Three animals were used in this study, proceeding from the University of Sao Paulo for the Laboratory of Morphology of the University Center of the Foundation of Education Octávio Bastos and one other directed by the IBAMA in accordance with process number 02027.000.286/2004-92, population excess of zoo, whose masculine reproductive devices "former situ" had been fixed by immersion and dissected. Each agency and attached gland of the reproductive device had been removed fragments of, which had been processed and enclosed by the techniques of inclusion in paraplast. Each block was cut and the cuts had been corados by HE, picrosírius, and histochemical reaction of PAS with deep of blue hematoxilina and Masson Tricrome for the comment of the structures to the optic microscope. Macroscopically, the inguinal region was composed by the urethra, isquiocavernosum muscle, bulbospongiosus and bulbcavernosum muscles, penis retractor muscle, a pair of testicles and penile bone or baculum and anus. The head of the penis presented a proximal dilatation constituted on the widest part of baculum. The position of the testes inside the scrotum was horizontal. The prostate gland was in globoid form surrounding the urethra. Microscopically, the testes were coated by dense connective tissue, the tunica albuginea. The ductus epididymis was coated by pseudostratified epithelia with stereocilia. The urethra of the penis was surrounded by the corpus spongiosum and the remaining portion presented a corpus cavernosum (erectile tissue). The found macro and microscopically results and until the moment are similar to the findings in the Cannis familiaris (domestic dog).
366. Morphological aspects of yolk sac from rodents of Hystricomorpha subordem: Paca (Agouti paca) and agouti (Dasyprocta aguti),Aspectos morfológicos do saco vitelino em roedores da subordem Hystricomorpha: Paca (Agouti paca) e cutia (Dasyprocta aguti)
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Conceição, R. A., Carlos Eduardo Ambrósio, Martins, D. S., Carvalho, A. F., Franciolli, A. L. R., Machado, M. R. F., Oliveira, M. F., and Miglino, M. A.
367. Characterization of the fetal membranes in buffalo cows in the first three months of gestation,Caracterização das membranas fetais em búfalas no terço inicial da gestação
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Morini, A. C., Barbosa, P. L. G., Melnic, R. V., João Carlos Jr, M., Franciolli, A. L. R., Daniele dos Santos Martins, Verechia Pereira, F. T., Favaron, P. O., Ambrósio, C. E., and Miglino, M. A.
368. β-lactam Antibiotics Active against Methicillin Resistant Staphylococcus aureus
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Moreillon, P., Franciolli, M., Cantoni, L., Bille, J., Glauser, M. P., Moreillon, P., Franciolli, M., Cantoni, L., Bille, J., and Glauser, M. P.
369. High-altitude pulmonary edema: findings at high-altitude chest radiography and physical examination.
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Vock, P, primary, Fretz, C, additional, Franciolli, M, additional, and Bärtsch, P, additional
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- 1989
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370. High altitude pulmonary edema after successful treatment of acute mountain sickness with dexamethasone
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Bärtsch, P., Vock, P., and Franciolli, M.
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- 1990
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371. Prevalence of functional dependence and chronic diseases in the community-dwelling Brazilian older adults: an analysis by dependence severity and multimorbidity pattern.
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Reis Júnior, Wanderley Matos, Ferreira, Luciano Nery, Molina-Bastos, Cynthia Goulart, Bispo Júnior, José Patrício, Reis, Helca Franciolli Teixeira, and Goulart, Bárbara Niegia Garcia
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Background: Despite the advancements in knowledge about health care for older adults, essential gaps persist regarding the effects of chronic diseases as epidemiological markers of the state of functional dependence. This study aimed to identify the prevalence of moderate and severe functional dependence in Brazilian older adults and its association with chronic diseases and verify the multimorbidity patterns by dependence status. Methods: This cross-sectional analytical study used data from 11,177 community-dwelling Brazilian older adults from the 2013 National Health Survey conducted in Brazil. The dependent variables were moderate and severe functional dependence in basic activities of daily living (BADLs) and instrumental ADLs (IADLs). The independent variables were defined based on the questions applied to measure each morbidity in a self-reported manner and asked, "Has a doctor ever diagnosed you as having (each disease)? Multimorbidity was simultaneously considered present for older adults with ≥ 2 chronic morbidities. The association between functional dependence on BADLs and IADLs separately by severity and the independent variables was verified from crude and adjusted estimates of the point prevalence ratios and their 95% confidence intervals using the regression model Poisson with robust variance. To group diseases into patterns, exploratory factor analysis was used. Results: The prevalences of moderate and severe BADL dependence were 10.2% (95% CI, 9.6–10.7) and 4.8% (95% CI, 4.4–5.2), respectively. Moderate and severe IADL dependence prevalences were 13.8% (95% CI, 13.1–14.4) and 15.6% (95% CI, 14.9%–16.2), respectively. When changing the condition from moderate to severe dependence in BADLs, in the presence of other mental illnesses and stroke, the probability of dependence increased more than four times in the case of other mental illnesses and more than five times for stroke. There was a linear trend for dependence severity, both moderate and severe, whereas, for severe dependence on IADLs, this same factor maintained a linear trend toward an increase in probability as the number of diseases simultaneously increased. Conclusions: Chronic diseases are associated with functional dependence, with greater emphasis on mental illnesses and stroke in severe disability, considering their acute adverse effects. [ABSTRACT FROM AUTHOR]
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- 2024
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372. MENTAL HEALTH CARE IN EMERGENCY MEDICAL SERVICES: AN INTEGRATIVE REVIEW.
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Bento Sabeh, Anna Carla, Domingues Wysocki, Anneliese, Gomes Campos, Claudinei José, Alvina dos Santos, Mariana, Teixeira Reis, Helca Franciolli, da Silva Kurimoto, Teresa Cristina, and Machado Dos-Santos, Edirlei
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PSYCHIATRIC nursing , *NURSING audit , *ONLINE information services , *CINAHL database , *ATTITUDES toward mental illness , *NURSING , *SYSTEMATIC reviews , *MEDICAL care , *EMERGENCY medical services , *COMMUNICATION , *DESCRIPTIVE statistics , *MEDLINE , *THEMATIC analysis , *PSYCHIATRIC emergencies , *MENTAL health services , *NURSING interventions - Abstract
Objective: to know the characteristics of mental health care provided by nursing professionals in emergency medical services. Method: integrative literature review in the databases Latin American and Caribbean Health Sciences Literature (LILACS), Publisher Medline (PUBMED), SciVerse Scopus (SCOPUS) and Cumulative Index to Nursing and Allied Health Literature (CINAHL), from March to June 2023, using previously defined inclusion and exclusion criteria. Results: the final sample included 59 articles, which were grouped into the following thematic categories: conceptions in mental health, communication, interventions, network care, competence in mental health care and barriers during care. Conclusion: care is done incipient and permeated by difficult elements. The findings of this study serve to review and rethink mental health care practices and policies and promote strategies for improving work processes. [ABSTRACT FROM AUTHOR]
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- 2023
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373. High altitude pulmonary edema after successful treatment of acute mountain sickness with dexamethasone
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Franciolli, M., Bartsch, P., and Vock, P.
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- 1990
374. β-lactam Antibiotics Active against Methicillin Resistant Staphylococcus aureus.
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Moreillon, P., Franciolli, M., Cantoni, L., Bille, J., and Glauser, M. P.
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- 1991
375. {beta}-Lactam Resistance Mechanisms of Methicillin-Resistant Staphylococcus aureus
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Franciolli, Mario, Bille, Jacques, Glauser, Michel R, and Moreillon, Philippe
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In vitro and in vivo activity of amoxicillin and penicillin G alone or combined with a penicillinase inhibitor (clavulanate) were tested against five isogenic pairs of methicillin-resistant Staphylococcus aureus (MRSA) producing or not producing penicillinase. Loss of the penicillinase plasmid caused an eight times or greater reduction in the MICs of amoxicillin and penicillin G (from 64 to 8 µg/ml), but not of the penicillinase-resistant drugs methicillin and cloxacillin (64 µg/ml). This difference in antibacterial effectiveness correlated with a more than 10 times greater penicillin-binding protein 2a affinity of amoxicillin and penicillin G than of methicillin and a 90% successful amoxicillin treatment of experimental endocarditis due to penicillinasenegative MRSA compared with cloxacillin, which was totally ineffective (P < .001). Amoxicillin was also effective against penicillinase-producing parent MRSA, provided it was combined with clavulanate. Penicillinase-sensitive β-lactam antibiotics plus penicillinase inhibitors might offer a rational alternative treatment for MRSA infections.
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- 1991
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376. {beta}-lactam Antibiotics Active against Methicillin Resistant Staphylococcus aureus
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Moreillon, P., Franciolli, M., Cantoni, L., Bille, J., and Glauser, M. P.
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- 1991
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377. Morphological study of larynx, trachea, and lungs of Didelphis marsupialis (LINNAEUS, 1758).
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Santos Pantoja, Bruna Tassia, Marques Silva, Armando Reinaldo, Mondego-Oliveira, Renata, Santos Silva, Thamires, Carvalho Marques, Babara, Pontes Albuquerque, Rafaela, Sá Sousa, Joicy Cortez, Grassi Rici, Rose Eli, Angélica Miglino, Maria, Lislea Sousa, Alana, Resende Franciolli, André Luís, Martins Sousa, Eduardo, Lúcia Abreu-Silva, Ana, and Cardoso Carvalho, Rafael
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MORPHOLOGY , *COMPARATIVE anatomy , *ANATOMY , *TRACHEA , *LARYNX , *TRACHEAL cartilage - Abstract
Background and Aim: From a biomedical point of view, the value of marsupials as a model of primitive mammals is indisputable. Among its species, the possum is a model that allows the study of the ontogeny of different organic systems, as well as their physiological aspects. The relevance of anatomical, functional, evolutionary, and phylogenetic study of marsupials for the development of comparative morphology is extensively documented in the literature. However, there are still many aspects to be further evaluated, as the anatomy and histology of the respiratory tract of this species. The aim of this study was to describe the morphology of the larynx, trachea, and lungs of Didelphis marsupialis. Materials and Methods: Five adult male animals were donated to the Comparative Animal Anatomy Laboratory - LAAC/CCAA-UFMA, for morphological studies. Specimens were washed in running water to perform biometrics. Then, they were fixed with 10% formaldehyde solution. After the fixation period, the specimens were positioned in dorsal decubitus position, for dissection of the respiratory system organs, by opening the ventral region of the neck and thoracic cavity, with subsequent removal of the pectoral muscles, ribs, and sternum. For histological analysis, fragments of 1 cm2 of the larynx (epiglottis and thyroid cartilages), trachea, and lungs were collected and fixed in 10% formaldehyde solution. Right after fixation, the fragments were dehydrated in increasing concentrations of ethyl alcohol (70, 80, 95, and 100%), diaphanized in xylene, embedded in paraffin, and sectioned into thin slices of 5 µm using a microtome. Sections were stained using the hematoxylin and eosin technique. Results: Anatomically, the larynx starts right after the pharynx. It consisted of four cartilages: Epiglottis, cricoid, thyroid, and arytenoid. The trachea was made of dorsally incomplete cartilaginous rings. At the entrance of the thoracic cavity, it bifurcated into the left and right main bronchus. The left lung was smaller than the right lung, with two lobes (cranial and caudal). The right lung presents the cranial, middle, caudal, and accessory lobes. Histologically, the epiglottis consisted of elastic cartilage and is covered by a non-keratinized stratified squamous epithelium. Thyroid cartilage is made of hyaline cartilage covered by smooth muscle. The trachea presents hyaline cartilage, with ciliated pseudo-stratified epithelium, serous glands, isogenic groups of chondrocytes, and perichondrium. The lung consisted of bronchi, bronchioles, and alveoli, also presenting blood vessels and arteries. Conclusion: Morphologically, the larynx, trachea, and lungs of D. marsupialis were similar to those of the other Didelphids described in the literature. [ABSTRACT FROM AUTHOR]
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- 2020
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378. Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment.
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Barreto, Rodrigo S. N., de Oliveira, Franceliusa Delys, Sá Schiavo Matias, Gustavo de, Rodrigues, Marcio N., Carvalho, Rafael C., Franciolli, André Luis R., Fratini, Paula, and Miglino, Maria Angelica
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EPITHELIAL cells , *VOMERONASAL organ , *CARTILAGE cells , *STEM cells , *MESENCHYMAL stem cells , *NEURAL stem cells , *OLFACTORY receptors - Abstract
The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromonesand emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim ofthis study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium. [ABSTRACT FROM AUTHOR]
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- 2020
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379. Obtaining animal protein in vitro
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Luana Felix de Melo, Rose Elí Grassi Rici, Christina Ramires Ferreira, Claudete Serrano Astolfi Ferreira, André Luis Rezende Franciolli, and Alan Peres Ferraz de Melo
- Abstract
Do ponto de vista fisiológico, as proteínas exercem fundamental importância no crescimento e manutenção da integridade das estruturas corporais e de suas funções, desempenhando essencial função plástica. A proteína animal é indispensável para a formação de órgãos e tecidos bem como seu funcionamento. Dessa forma, objetivou-se a produção de proteína “in vitro” utilizando músculo bíceps femoral bovinos os quais foram utilizados para cultivo celular, descelularização e posterior recelularização, comparando entre amostras de adulto e feto. A metodologia utilizada foi através de técnicas macroscópicas, microscópicas de luz e eletrônica de varredura e molecular. Os resultados demonstraram que os protocolos foram padronizados tanto para controle quanto descelularização e recelularização. Comparando os grupos de amostras de adultos e fetos, foi possível observar que o melhor protocolo para a obtenção da carne in vitro, é utilizando o arcabouço do músculo adulto com as células de origem fetal. O arcabouço obtido foi capaz de sinalizar e apresentar uma estrutura viável para receber as células fetais, promovendo uma biocompatibilidade, propiciando o crescimento e expensão celular por todo arcabouço. A análise molecular confirmou a amplificação em qPCR detectando os genes de miogenina (MYOG), miosina de cadeia pesada (MyHC) e o gene endógeno (GAPDH), confirmando assim a presença de proteína e a viabilidade da produção in vitro. From a physiological point of view, proteins play a fundamental role in the growth and maintenance of the integrity of body structures and their functions, performing an essential plastic function. Animal protein is essential for the formation of organs and tissues as well as their functioning. Thus, the objective was to produce protein “in vitro” using bovine biceps femoris muscle, which were used for cell culture, decellularization and subsequent recellularization, comparing between adult and fetal samples. The methodology used was through macroscopic, light microscopic and scanning and molecular electronic techniques. The results showed that the protocols were standardized for both control and decellularization and recellularization. Comparing the groups of samples of adults and fetuses, it was possible to observe that the best protocol for obtaining meat in vitro is using the adult muscle framework with cells of fetal origin. The scaffold obtained was able to signal and present a viable structure to receive fetal cells, promoting biocompatibility, promoting cell growth and expansion throughout the scaffold. Molecular analysis confirmed the amplification in qPCR detecting the myogenin (MYOG), myosin heavy chain (MyHC) and the endogenous gene (GAPDH), thus confirming the presence of protein and the viability of production in vitro.
- Published
- 2022
380. Trends of the Epidemiology of Candidemia in Switzerland: A 15-Year FUNGINOS Survey
- Author
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Kai-Manuel, Adam, Michael, Osthoff, Frédéric, Lamoth, Anna, Conen, Véronique, Erard, Katia, Boggian, Peter W, Schreiber, Stefan, Zimmerli, Pierre-Yves, Bochud, Dionysios, Neofytos, Mapi, Fleury, Hans, Fankhauser, Daniel, Goldenberger, Konrad, Mühlethaler, Arnaud, Riat, Reinhard, Zbinden, Andreas, Kronenberg, Chantal, Quiblier, Oscar, Marchetti, Nina, Khanna, University of Zurich, Khanna, Nina, Fungal Infection Network of Switzerland (FUNGINOS), Bregenzer, T., Conen, A., Adam, K.M., Flückiger, U., Khanna, N., Orasch, C., Heininger, U., Franciolli, M., San Giovanni, O., Damonti, L., Zimmerli, S., Rothen, M., Zellweger, C., Tarr, P., Fleisch, F., Chuard, C., Erard, V., Emonet, S., Garbino, J., Neofytos, D., van Delden, C., Genne, D., Bochud, P.Y., Calandra, T., Lamoth, F., Marchetti, O., Chave, J.P., Bois-Cerf, C., Cécil, C., La Source, C., Graber, P., Monotti, R., Regionale, O., Bernasconi, E., Civico, O., Rossi, M., Krause, M., Piso, R.J., Bally, F., Troillet, N., Boggian, K., Eich, G., Gubler, J., Fehr, J., Imhof, A., Ruef, C., Werner Schreiber, P., Berger, C., Fankhauser, H., Heinzer, I., Goldenberger, D., Frei, R., Hertel, R., Dolina, M., Petrini, O., Dubuis, O., Mühlethaler, K., Graf, S., Risch, M., Ritzler, E., Fracheboud, D., Riat, A., Rohner, P., Schrenzel, J., Lienhardt, R., Bille, J., Andreutti-Zaugg, C., Gallusser, A., Pfyffer, G., Herzog, K., Schibli, U., Tissière, L., Bruderer, T., and Zbinden, R.
- Subjects
medicine.medical_specialty ,Population ,610 Medicine & health ,resistance ,10234 Clinic for Infectious Diseases ,Internal medicine ,Intensive care ,medicine ,Major Article ,education ,Candida albicans ,education.field_of_study ,biology ,Candida glabrata ,business.industry ,10179 Institute of Medical Microbiology ,Incidence (epidemiology) ,candidemia ,Micafungin ,biology.organism_classification ,bacterial infections and mycoses ,Infectious Diseases ,AcademicSubjects/MED00290 ,2728 Neurology (clinical) ,Oncology ,antifungals ,candida ,epidemiology ,Anidulafungin ,570 Life sciences ,2730 Oncology ,business ,Fluconazole ,medicine.drug - Abstract
Background The increasing incidence of candidemia and emergence of drug-resistant Candida species are major concerns worldwide. Long-term surveillance studies are needed. Methods The Fungal Infection Network of Switzerland (FUNGINOS) conducted a 15-year (2004–2018), nationwide, epidemiological study of candidemia. Hospital-based incidence of candidemia, Candida species distribution, antifungal susceptibility, and consumption were stratified in 3 periods (2004–2008, 2009–2013, 2014–2018). Population-based incidence over the period 2009–2018 derived from the Swiss Antibiotic Resistance Surveillance System (ANRESIS). Results A total of 2273 Candida blood isolates were studied. Population and hospital-based annual incidence of candidemia increased from 2.96 to 4.20/100 000 inhabitants (P = .022) and 0.86 to 0.99/10 000 patient-days (P = .124), respectively. The proportion of Candida albicans decreased significantly from 60% to 53% (P = .0023), whereas Candida glabrata increased from 18% to 27% (P < .0001). Other non-albicans Candida species remained stable. Candida glabrata bloodstream infections occurred predominantly in the age group 18–40 and above 65 years. A higher proportional increase of C glabrata was recorded in wards (18% to 29%, P < .0001) versus intensive care units (19% to 24%, P = .22). According to Clinical and Laboratory Standards Institute, nonsusceptibility to fluconazole in C albicans was observed in 1% of isolates, and anidulafungin and micafungin nonsusceptibility was observed in 2% of C albicans and C glabrata. Fluconazole consumption, the most frequently used antifungal, remained stable, whereas use of mold-active triazoles and echinocandins increased significantly in the last decade (P < .0001). Conclusions Over the 15-year period, the incidence of candidemia increased. A species shift toward C glabrata was recently observed, concurring with increased consumption of mold-active triazoles., The incidence of candidemia increased in Switzerland from 2004 to 2018. A species shift toward C glabrata was observed after 2013, now accounting for one fourth of all candidemia, concurring with increased consumption of mold-active triazoles.
- Published
- 2021
381. Technologies applied to the identification of the role of lipids during organogenesis in mammals (swine and canine models)
- Author
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Marisol León Cabrera, Maria Angélica Miglino, André Luis Rezende Franciolli, and William David Pérez González
- Abstract
O desenvolvimento embrionário encontra-se dividido em três etapas nas quais podem ser distinguidas as três estruturas: ovo, embrião e feto. Durante o desenvolvimento ocorrem eventos distintos e alterações morfológicas que dão inicio à vida. Os lipídios desempenham um papel importante no processo de formação, pois tem uma participação específica no desenvolvimento embrionário. A utilização de um conjunto de técnicas poderia auxiliar e decrever melhor os processos de formação e a sinalização molecular do desenvolvimento. Porém, o objetivo proposto neste trabalho foi caracterizar de forma aprimorada (mediante tecnologias apropriadas) o desenvolvimento embrionário de mamíferos, em relação à identificação de grupos lipídios. Para isso, foram estudados 28 fetos e embriões de cães, com idades compreendidas entre os 22 e 55 dias de gestação, obtidos em campanha de castração, além de um feto de suíno de 50 dias de gestação, procedente de um fornecedor comercial (Animal Technologies, Inc.). Foram analisados fígado, coração, cérebro, rins e pulmões de embriões e fetos, valendo-se de tecnologias de imagem (Microscopia Eletrônica de Varredura, Tomografia Computadorizada, Raios-X), e técnicas histológicas (colorações de Hematoxilina e Eosina, Tricrômio de Masson, Ferro Coloidal, Sudan Black) em cães. No suíno realizou-se além das citadas técnicas, a análise lipidômica relizada pela técnica de Electroespraiamento por Dispersão- Imageamento por Espectrometria (DESI-MS Imagem) e, posteriormente, coloração com Hematoxilina e Eosina. As principais conclusões relacionam-se ao emprego de várias tecnologias para descrever o desenvolvimento embrionário e fetal, caracterizando bem o desenvolvimento de estruturas, tais como: os arcos braquiais e as vesículas ópticas e óticas nos embriões, cordas tendíneas no coração, lobos hepáticos e mineralização do tecido ósseo. A vinculação dos lipídios à essas tecnologias permitiu associá-las aos processos de contração miocárdica, formação de surfactante pulmonar e trânsito de metabólitos das células epiteliais. Além disso, permitiram ainda demostrar as distribuições lipídicas em órgãos: Nacetilglutamina no coração, ST (ht22: 0) nos intestinos, AG dímeros no fígado e um amplo número de lipídios PS no sistema nervoso. Conclui-se que, de modo geral, existe grande variabilidade na interação do papel dos lipídios com o desenvolvimento embrionário. Embryonic development is divided into three stages in which the three structures can be distinguished; egg, embryo and fetus. During development occur distinct events, and morphological changes that start life. Lipids play an important role in the formation process because they have a specific participation in embryonic development. The use of a set of techniques could help and improve the processes of formation and the molecular sign of embryonic development. However, the objective of this work was to characterize the embryonic development of mammals in an improved way (through appropriate technologies), in relation to the identification of lipid groups. For this, 28 fetuses and embryos of dogs, aged between 22 and 55 days of gestation, obtained in a castration campaign, in addition to a fetus of 50 days\' gestation from a commercial supplier (Animal Technologies, Inc.). The liver, heart, brain, kidneys and lungs of embryos and fetuses were analyzed using imaging technologies (Scanning Electron Microscopy, Computed Tomography, X-rays) and histological techniques (Hematoxylin and Eosin stains, Masson\'s Tricomial , Colloidal Iron, Sudan Black) in dogs. In the pig, all the techniques were performed, the lipid analysis revealed by the technique of Dispersion Electrospray - Spectrometry Imaging (DESI-MS Image) and subsequently staining with Hematoxylin and Eosin. The main conclusions are related to the use of several technologies to describe embryonic and fetal development, characterizing well the development of structures such as brachial arches and optic and optical vesicles in embryos, chordae tendineae in the heart, hepatic lobes and tissue mineralization bone. The lipid binding to these technologies has been associated with the processes of myocardial contraction, formation of pulmonary surfactant and transit of epithelial cell metabolites. In addition, they allowed to demonstrate the lipid distributions in organs: specific distributions were shown in organs: N-acetylglutamine in the heart, ST (ht22: 0) in the intestines, AG dimers in the liver, and a large number of PS lipids in the nervous system. We conclude that in general there is great variability in the interaction of the role of lipids with embryonic development.
- Published
- 2018
382. Análise estereológica de neurônios do corpo amigdaloide e avaliação comportamental de camundongos sob o uso de esteroides anabolizantes
- Author
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RIBEIRO, Melissa Siqueria, NASCIMENTO, Carlos Giovani de Oliveira, ESTEVES, Alessandra, ROSSI JUNIOR, Wagner Costa, and FRANCIOLLI, André Luis Rezende
- Subjects
Neurônios ,Esteroides ,Animais - Comportamento agressivo ,Tonsila do Cerebelo ,Ansiedade ,ANATOMIA [MORFOLOGIA] - Abstract
Os esteróides anabólico-androgênicos (EAAs) são derivados sintéticos da testosterona utilizados de maneira abusiva por indivíduos que procuram ganho de força física ou apenas efeitos estéticos. Doses suprafisiológicas destes compostos têm sido associadas a possíveis danos em tecido nervoso, a efeitos emocionais e comportamentais adversos, como alteração nas respostas de ansiedade ou agressividade. Considerando o desconhecimento dos aspectos neurais envolvidos nestas alterações comportamentais, e os resultados de trabalhos que apontam para um possível efeito deletério dos EAAs no Sistema nervoso central (SNC), investigamos a integridade do número de neurônios da amígdala basolateral (ABL) e do núcleo póstero-ventral da amígdala medial (MePV) de camundongos machos e fêmeas. Os animais receberam injeções intraperitoneais de cipionato de testosterona (Deposteron®) e estanozolol (Winstrol Depot ®) em doses suprafisiológicas, duas vezes por semana, no período de 33 dias. Assim como um grupo controle recebeu solução salina estéril a 0,9% nos mesmos dias. Foi realizada a análise numérica de perfis de corpos celulares de neurônios para a quantificação dos mesmos e testes comportamentais. Os animais tratados apresentaram respostas ansiogênicas no teste do labirinto em cruz elevado (LCE). No entanto, não foram observadas alterações na atividade motora geral em campo aberto (CA) e nos parâmetros de agressividade no teste de modelo residente-intruso. Os dois EAAs foram capazes de reduzir significantemente a quantidade de corpos celulares de neurônios nas duas áreas analisadas, sugerindo um processo de morte neuronal induzido pelos EAAs. The androgenic-anabolic steroids (AAS) are synthetic derivatives of testosterone abusively used by individuals looking to gain physical strength or aesthetic purposes. Supraphysiological doses of these compounds have been associated with possible damages to nervous tissue, emotional and behavioral adverse effects, such as changes in anxious or aggressive responses. Considering the lack of knowledge about the neural aspects involved in these behavioral changes, and researchs that point to a possible deleterious effect of AAS in the central nervous system (CNS), we investigated the integrity of the number of cell bodies of neurons of the basolateral amygdala (BLA) and the posteroventral medial amygdala nucleus (MePV) of male and female mice. The animals received intraperitoneal injections of testosterone cypionate (Deposteron ®) and stanozolol (Winstrol Depot ®) supraphysiological doses, twice a week in the period of 33 days. As a control group received 0.9% sterile saline solution on the same days. For quantification and behavioral tests, analyses on the number of cell bodies of neurons was performed. The treated mice showed an increase in anxiety-related behaviors in the elevated plus maze test, and no changes were observed in the general motor activity in the open field and parameters of aggressiveness in the resident-intruder test model. The two AAS were able to significantly reduce the number of neuronal cell bodies in both analyzed areas, suggesting a process of neuronal death due to apoptosis induced by the AAS. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
- Published
- 2014
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