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In chronic lymphocytic leukaemia (CLL) clinical course of patients is heterogeneous. Some present an aggressive disease onset and require immediate therapy, while others remain without treatment for years. Current disease staging systems developed by Rai and Binet may be useful in forecasting patient survival time, but do not discriminate between stable and progressive forms of the disease in the early stages. Recently ample attention has been directed towards identifying new disease prognostic markers capable of predicting clinical aggressiveness at diagnosis. In this research we reached for LC-MS metabolic fingerprinting method to analyse serum of stable (n=51) and progressive (n=42) CLL patients and controls (n=45) with aim to discover metabolic indicators of disease status. A panel of markers discriminating aggressive from indolent patients was discovered. Ten of them were selected for validation on larger population (45 controls and 92 CLL) with an independent analytical technique. Linoleamide (p=0.002) in addition to various acylcarnitines (p=0.001-0.000001) showed to be significant markers of CLL in its aggressive form. Acetylcarnitine (p=0.05) and hexannoylcarnitine (p=0.005) were also distinguishable markers of indolent subjects. Forming a panel of selected acylcarnitines and fatty acid amides, it was possible to reach a highly specific and sensitive diagnostic approach (AUC=0.766). Disclosures No relevant conflicts of interest to declare.

En: Chemistry and econology. ISSN. 0275-7540. n. 16 (1999), p. 297-316 A screening often different Mediterranean crops was carried out to test their tolerance to soils polluted with copper and zinc. The methods for copper and zinc determination in plants have been improved, avoiding the time consuming acid digestion. Results showed that zinc toxicity was higher than copper for the assayed plants. In copper-polluted soils, Lupinus luteus (lupin) 549µgg- 1 and Zea mays (corn) 213µgg- 1 showed the highest amounts dried weight of accumulated metal, mainly detected associated to the root system without significant reduction of biometrical parameters of the plants. In zinccontaminated soils, corn (1304 µgg- 1 ), Triticum durum (wheat) (933 µgg- 1 ) and lupin (654µgg- 1 ) showed the highest amounts of metal accumulated mainly in the roots, and in all cases, without biometrical reduction.

Acute lung injury induced by mechanical ventilation [ventilator-induced lung injury (VILI)]is characterized by a particular metabolic profile in the lung and in the systemic compartment [1]. Also, VILI has been associated with an increase in intestinal permeability [2]. We hypothesized that selective decontamination of the digestive tract (SDD) can modulate the metabolic profile associated with mechanical ventilation.

© The authors, Two of the largest crude oil-polluted areas in the world are the semi-enclosed Mediterranean and Red Seas, but the effect of chronic pollution remains incompletely understood on a large scale. We compared the influence of environmental and geographical constraints and anthropogenic forces (hydrocarbon input) on bacterial communities in eight geographically separated oil-polluted sites along the coastlines of the Mediterranean and Red Seas. The differences in community compositions and their biodegradation potential were primarily associated (Pâ €‰, This research was supported by the European Community Projects MAGICPAH (FP7-KBBE-2009-245226), ULIXES (FP7-KBBE-2010-266473) and KILLSPILL (FP7-KBBE-2012-312139). We thank EU Horizon 2020 Program for the support of the Project INMARE H2020-BG-2014-2634486. This work was further funded by grants BIO2011-25012, PCIN-2014-107 and BIO2014-54494-R from the Spanish Ministry of Economy and Competitiveness. The authors gratefully acknowledge the financial support provided by the European Regional Development Fund (ERDF). The present investigation was also funded by the Spanish Ministry of Economy and Competitiveness within the ERA NET IB2, grant number ERA-IB-14-030. F. Mapelli was supported by Università degli Studi di Milano, European Social Fund (FSE) and Regione Lombardia (contract “Dote Ricerca”). CB and DR would like to acknowledge funding from the Spanish Ministry of the Economy and Competitiveness (CTQ2014-55279-R).