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Stevia rebaudiana (Bertoni) is a highly valuable crop for the steviol glycoside content in its leaves, which are no-calorie sweeteners hundreds of times more potent than sucrose. The presence of health-promoting phenolic compounds, particularly flavonoids, in the leaf of S. rebaudiana adds further nutritional value to this crop. Although all these secondary metabolites are highly desirable in S. rebaudiana leaves, the genes regulating the biosynthesis of phenolic compounds and the shared gene network between the regulation of biosynthesis of steviol glycosides and phenolic compounds still need to be investigated in this species. To identify putative candidate genes involved in the synergistic regulation of steviol glycosides and phenolic compounds, four genotypes with different contents of these compounds were selected for a pairwise comparison RNA-seq analysis, yielding 1136 differentially expressed genes. Genes that highly correlate with both steviol glycosides and phenolic compound accumulation in the four genotypes of S. rebaudiana were identified using the weighted gene co-expression network analysis. The presence of UDP-glycosyltransferases 76G1, 76H1, 85C1, and 91A1, and several genes associated with the phenylpropanoid pathway, including peroxidase, caffeoyl-CoA O-methyltransferase, and malonyl-coenzyme A:anthocyanin 3-O-glucoside-6″-O-malonyltransferase, along with 21 transcription factors like SCL3, WRK11, and MYB111, implied an extensive and synergistic regulatory network involved in enhancing the production of such compounds in S. rebaudiana leaves. In conclusion, this work identified a variety of putative candidate genes involved in the biosynthesis and regulation of particular steviol glycosides and phenolic compounds that will be useful in gene editing strategies for increasing and steering the production of such compounds in S. rebaudiana as well as in other species. [ABSTRACT FROM AUTHOR]

We identified and characterized the pseudogene complements of five plant species: four dicots (Arabidopsis thaliana, Vitis vinifera, Populus trichocarpa and Phaseolus vulgaris) and one monocot (Oryza sativa). Retroposition was considered of modest importance for pseudogene formation in all investigated species except V. vinifera, which showed an unusually high number of retro-pseudogenes in non coding genic regions. By using a pipeline for the classification of sequence duplicates in plant genomes, we compared the relative importance of whole genome, tandem, proximal, transposed and dispersed duplication modes in the pseudo and functional gene complements. Pseudogenes showed higher tendencies than functional genes to genomic dispersion. Dispersed pseudogenes were prevalently fragmented and showed high sequence divergence at flanking regions. On the contrary, those deriving from whole genome duplication were proportionally less than expected based on observations on functional loci and showed higher levels of flanking sequence conservation than dispersed pseudogenes. Pseudogenes deriving from tandem and proximal duplications were in excess compared to functional loci, probably reflecting the high evolutionary rate associated with these duplication modes in plant genomes. These data are compatible with high rates of sequence turnover at neutral sites and double strand break repairs mediated duplication mechanisms. [ABSTRACT FROM AUTHOR]

The relationship between variation of the repetitive component of the genome and domestication in plant species is not fully understood. In previous work, variations in the abundance and proximity to genes of long terminal repeats (LTR)-retrotransposons of sunflower (Helianthus annuus L.) were investigated by Illumina DNA sequencingtocompare cultivars and wild accessions. In this study, we annotated and characterized 22 specific retrotransposon families whose abundance varies between domesticated and wild genotypes. These families mostly belonged to the Chromovirus lineage of the Gypsy superfamily and were distributed overall chromosomes. They were also analyzed in respect to their proximity to genes. Genes close to retrotransposon were classified according to biochemical pathways, and differences between domesticated and wild genotypes are shown. These data suggest that structural variations related to retrotransposons might have occurred to produce phenotypic variation between wild and domesticated genotypes, possibly by affecting the expression of genes that lie close to inserted or deleted retrotransposons and belong to specific biochemical pathways as those involved in plant stress responses. [ABSTRACT FROM AUTHOR]

In this paper, a comparative study including measurement and modeling of partial discharge for various void geometries (cylinder, block, and prisms) at very low frequency (VLF) and power frequency (PF) voltage is presented. Results from experiments and simulations are presented with phase-resolved discharge patterns and integrated parameters (maximum/average discharge magnitudes, and repetition rates). Dynamic simulation, achieved using a combination of finite element analysis and Matlab, matches the experimental results. Differences of discharge characteristics between VLF and PF may be attributed to the physical mechanisms in the modeling including cavity surface conductivity and space charge decay rate. Compared with discharge activities at PF, the VLF excitation in general exhibits relatively lower discharge magnitude and repetition rate. Also, the inception voltage is found to be lower at VLF. [ABSTRACT FROM AUTHOR]

The most effective method for insulation assessment in an electrical power apparatus is partial discharge (PD) detection. During measurements, the interference from the background environment hampers the PD signal and reduces its measurement accuracy. This article discusses on the implementation of a Hankel matrix (HM)-based fast singular value decomposition (H-FSVD) technique for removing noise from the PD signals. The data are first represented into HM structure, with appropriate sampling then using the Lanczos process, the HM size is reduced and through singular spectral analysis thresholds are fixed for noise detection and removal. This algorithm has been examined on simulated as well as PD signals measured on two different laboratory environments from transformers with real and simulated noise. The experiment is part of a series of experiments to detect PD patterns related to realistic transformer defects. The denoising performance of H-FSVD is compared with the wavelet-based denoising methods, empirical mode decomposition method, and normal SVD. Numerical results show that H-FSVD efficiently removes the noise with less computation time, even for large size data. [ABSTRACT FROM AUTHOR]

Detection and measurement of partial discharge (PD) phenomena combined with the separation and identification of PD sources is the way to achieve effective insulation integrity assessment. However, during measurement, PD signals are coupled with interferences (discrete spectral, pulsive, and white noises). Recovering PD signals from such interferences would improve PD source separation (thus identification), but still remains a challenging task. Several denoising methods have been proposed to suppress interferences. However, using a universal method to achieve interference removal is probably impossible, as the characteristics of the interferences are distinct. This study proposes a novel low‐rank H‐Matrix‐based singular value decomposition (SVD) filter (H‐SVD) that removes different types of interferences. Denoising is done by projecting the measured pulse in a lower dimensional signal space. To assess the effectiveness of the proposed method, H‐SVD filter is first applied to simulated PD data and later on real‐time PD data with the introduction of three different types of synthetic noises. The results of the evaluation metrics confirm that H‐SVD has significant performance improvements compared to existing state‐of‐the‐art PD denoising methods. [ABSTRACT FROM AUTHOR]

An interdigitated capacitor embedded in the EMC has been realized and characterized under controlled humidity conditions by applying a DC step voltage and monitoring the leakage current as a function of time. Both experimental characterization and TCAD simulations of dry and wet EMC samples have been carried out to fully understand the involved charge transport mechanisms. The anomalous increase of current with time in wet conditions is explained assuming a build-up of space charge directly induced by the high-injection at the metal electrodes. The assumption is confirmed by TCAD simulations. • Electrical characterization of EMC capacitors under both dry and wet conditions. • Poole-Frenkel mobility model reproduce the EMC conductivity field dependence. • Strong current increase with timein Wet EMC samples subjected to DC stress. • Schottky contacts with barrier lowering accounts for the current time dependence. [ABSTRACT FROM AUTHOR]

Softening processes after ripening are a major factor contributing to the perishability of fleshy fruit and, together with mechanical damage, represent the onset of physiological decay. Softening involves multiple co-ordinated events leading to modifications of the cell wall architecture. Several studies described that UV-B radiation positively affects both the nutraceutical and aesthetical qualities of fruit. However, very few studies investigated the effect of UV-B irradiation on the activity of cell wall-related enzymes. This research aimed at studying how different UV-B treatments (10 min and 60 min) affect the activity of cell wall-modifying enzymes (pectin methylesterase, polygalacturonase and β-galactosidase) together with the expression of some of their isoforms up to 36 h after UV-B treatment of peach (cv. Fairtime, melting phenotype) fruit. Results revealed that UV-B radiation did not affect the soluble solid content and the titratable acidity, two important parameters influencing consumers’ choice and taste. In contrast, UV-B was effective at reducing the loss of firmness 24 h after the 60 min irradiation. Generally, a lower activity of the hydrolytic enzymes compared to untreated fruit was observed, regardless of the UV-B dose. However, gene expression did not reflect the corresponding enzymatic activity. Based on these results, UV-B irradiation might be a successful tool in reducing the loss of firmness of peach fruit during post-harvest, thus improving their quality and shelf-life. [ABSTRACT FROM AUTHOR]

A reply to a discussion on the effect of supply voltage frequency on testing of insulation system is presented.

Development of rapid and multiplexed diagnostic tools is a top priority to address the current epidemic problem of sexually transmitted diseases. Here we introduce a novel nanoplasmonic biosensor for simultaneous detection of the two most common bacterial infections: Chlamydia trachomatis and Neisseria gonorrhoeae . Our plasmonic microarray is composed of gold nanohole sensor arrays that exhibit the extraordinary optical transmission (EOT), providing highly sensitive analysis in a label-free configuration. The integration in a microfluidic system and the precise immobilization of specific antibodies on the individual sensor arrays allow for selective detection and quantification of the bacteria in real-time. We achieved outstanding sensitivities for direct immunoassay of urine samples, with a limit of detection of 300 colony forming units (CFU)/mL for C. trachomatis and 1500 CFU/mL for N. gonorrhoeae . The multiplexing capability of our biosensor was demonstrated by analyzing different urine samples spiked with either C. trachomatis or N. gonorrhoeae , and also containing both bacteria. We could successfully detect, identify and quantify the levels of the two bacteria in a one-step assay, without the need for DNA extraction or amplification techniques. This work opens up new possibilities for the implementation of point-of-care biosensors that enable fast, simple and efficient diagnosis of sexually transmitted infections. [ABSTRACT FROM AUTHOR]

The WUSCHEL-related homeobox (WOX) is a family of specific transcription factors involved in plant development and response to stress, characterized by the presence of a homeodomain. This study represents the first comprehensive characterization of the WOX family in a member of the Asteraceae family, the sunflower (H. annuus L.). Overall, we identified 18 putative HaWOX genes divided by phylogenetic analysis in three major clades (i.e., ancient, intermediate, and WUS). These genes showed conserved structural and functional motifs. Moreover, HaWOX has homogeneously distributed on H. annuus chromosomes. In particular, 10 genes originated after whole segment duplication events, underpinning a possible evolution of this family along with the sunflower genome. In addition, gene expression analysis evidenced a specific pattern of regulation of the putative 18 HaWOX during embryo growth and in ovule and inflorescence meristem differentiation, suggesting a pivotal role for this multigenic family in sunflower development. The results obtained in this work improved the understanding of the WOX multigenic family, providing a resource for future study on functional analysis in an economically valuable species such as sunflower. [ABSTRACT FROM AUTHOR]

SUMMARY: Transposable elements (TEs) are an important source of genome variability, playing many roles in the evolution of eukaryotic species. Besides well‐known phenomena, TEs may undergo the exaptation process and generate the so‐called exapted transposable element genes (ETEs). Here we present a genome‐wide survey of ETEs in the large genome of sunflower (Helianthus annuus L.), in which the massive amount of TEs, provides a significant source for exaptation. A library of sunflower TEs was used to build TE‐specific Hidden Markov Model profiles, to search for all available sunflower gene products. In doing so, 20 016 putative ETEs were identified and further investigated for the characteristics that distinguish TEs from genes, leading to the validation of 3530 ETEs. The analysis of ETEs transcription patterns under different stress conditions showed a differential regulation triggered by treatments mimicking biotic and abiotic stress; furthermore, the distribution of functional domains of differentially regulated ETEs revealed a relevant presence of domains involved in many aspects of cellular functions. A comparative genomic investigation was performed including species representative of Asterids and appropriate outgroups: the bulk of ETEs that resulted were specific to the sunflower, while few ETEs presented orthologues in the genome of all analyzed species, making the hypothesis of a conserved function. This study highlights the crucial role played by exaptation, actively contributing to species evolution. Significance Statement: The molecular domestication of transposable elements leads to the formation of novel genes through exaptation. By setting up a protocol for systematically discovering exapted transposable elements (ETEs) in large genome species, we identified and validated 3,530 ETEs in sunflower genome. The identification of ETEs in Helianthus annuus can be considered further proof of the fundamental contribution that TEs had in the rising of genetic novelties, probably influencing different biological processes during the evolution of the sunflower. [ABSTRACT FROM AUTHOR]

Blackberries (Rubus spp.) are the fourth most economically important berry crop worldwide. Genome assemblies and annotations have been developed for Rubus species in subgenus Idaeobatus, including black raspberry (R. occidentalis), red raspberry (R. idaeus), and R. chingii, but very few genomic resources exist for blackberries and their relatives in subgenus Rubus. Here we present a chromosomelength assembly and annotation of the diploid blackberry germplasm accession "Hillquist" (R. argutus). "Hillquist" is the only known source of primocane-fruiting (annual-fruiting) in tetraploid fresh-market blackberry breeding programs and is represented in the pedigree of many important cultivars worldwide. The "Hillquist" assembly, generated using Pacific Biosciences long reads scaffolded with high-throughput chromosome conformation capture sequencing, consisted of 298Mb, of which 270Mb (90%) was placed on 7 chromosome-length scaffolds with an average length of 38.6Mb. Approximately 52.8% of the genome was composed of repetitive elements. The genome sequence was highly collinear with a novel maternal haplotype-resolved linkage map of the tetraploid blackberry selection A-2551TN and genome assemblies of R. chingii and red raspberry. A total of 38,503 protein-coding genes were predicted, of which 72% were functionally annotated. Eighteen flowering gene homologs within a previously mapped locus aligning to an 11.2Mb region on chromosome Ra02 were identified as potential candidate genes for primocane-fruiting. The utility of the "Hillquist" genome has been demonstrated here by the development of the first genotyping-by-sequencing-based linkage map of tetraploid blackberry and the identification of possible candidate genes for primocane-fruiting. This chromosome-length assembly will facilitate future studies in Rubus biology, genetics, and genomics and strengthen applied breeding programs. [ABSTRACT FROM AUTHOR]

In the framework of developing defect-based life models, in which breakdown is explicitly associated with partial discharge (PD)-induced damage growth from a defect, ageing tests and PD measurements were carried out in the laboratory on polyethylene (PE) layered specimens containing artificial cavities. PD activity was monitored continuously during aging. A quasi-deterministic series of stages can be observed in the behavior of the main PD parameters (i.e. discharge repetition rate and amplitude). The evolution of the parameters reflects the physicalchemical changes taking place at the dielectric/cavity interface during the aging process. PD activity shows similar time behavior under constant cavity gas volume and constant cavity gas pressure conditions, suggesting that the variation of PD parameters may not be attributed to the variation of the gas pressure. It is speculated that the change of PD activity is related to the composition of the cavity gas, as well as to the properties of dielectric/cavity interface. [ABSTRACT FROM AUTHOR]

Experiments on the breakdown characteristics of a long air gap containing a floating conductor under switching impulse are carried out to address issues in live-line work engineering. The effects of gap distance, floating conductor structure, wavefront time, and high altitude on breakdown voltage are investigated, respectively. The results show that a floating conductor with sharp protrusions reduces the breakdown voltage and produces the lowest breakdown voltage area (LBA). The maximum reduction rate of the breakdown voltage and the position of the LBA vary with the gap distance. The position of the LBA is also related to the floating conductor structure. The results indicate that different floating conductor structures with similar electric field distortions have a similar effect on the breakdown voltage. For the effect of wavefront time on breakdown characteristics, the results show that the breakdown voltage under switching impulse with a long wavefront time is higher than that under a standard switching impulse. For the effect of high altitude, the results show that the LBA at high altitude is different from that at low altitude, and the high-altitude breakdown voltage corrected using the International Electrotechnical Commission (IEC) altitude correction method is not exactly the same as the actual experimental results. [ABSTRACT FROM AUTHOR]

• AMF outperforms phosphate in favouring nutrient uptake. • AMF boosts photosynthesis & secondary metabolism. • Transcriptome analysis reveals molecular bases of several AMF effects. • AMF triggers distinct gene expression compared to phosphate. • Gene expression correlates with plant traits. Lettuce (Lactuca sativa L.) is an annual autogamous diploid plant belonging to Asteraceae and is among the top ten most profitable crops worldwide due to its nutritional value. Nevertheless, lettuce is an intensive crop with high input demand, especially fertilisers, both in soil-bound and soilless culture, with negative impact on the environment and production costs. In this context, arbuscular mycorrhizal symbioses could help to reduce the impact of fertilisers, improving plant nutrition, particularly regarding phosphorus, and contributing to growing healthier plants for human consumption. In our work, we compared lettuce plants (cv. Salinas) grown in soilless culture with optimal phosphorus concentration in the nutrient solution, with sub-optimal phosphorus nutrition and with plants grown with sub-optimal phosphorus concentration and inoculated with the fungus Funneliformis mosseae before transplanting. Higher levels of primary and secondary metabolites along with increased biomass were observed for mycorrhizal plants compared to lettuces grown with optimal and sub-optimal concentrations of phosphate. Gene expression profile was analysed in both roots and leaves, and transcriptomic values were associated with growth and biochemical parameters. Three-thousand and fifty-seven genes were differentially regulated by mycorrhizal symbiosis and 2,606 genes by optimal phosphate nutrition. Different genes related to photosynthesis, solute transport, metabolism of phytohormones, redox homeostasis, and transcriptional regulation resulted differentially regulated between culture conditions. Mycorrhizal plants also boosted the activation of genes involved in phenylpropanoids and carotenoids metabolism. In conclusion, growth, biochemical, and transcriptomic data show that symbiotic plants benefit both plant growth and leaf content of health promoting phytochemicals through genetic pathways that largely differed those activated in plants grown with optimal phosphorus supply. [ABSTRACT FROM AUTHOR]

On average, the genomes of Asteraceae display a high proportion of TEs (70%), representing an excellent system to study plant genome evolution ([11]). Keywords: Asteraceae; Database; Transposable elements annotation EN Asteraceae Database Transposable elements annotation 365 367 3 04/19/23 20230401 NES 230401 Transposable elements (TEs) are interspersed repetitive DNA sequences that can move independently within the genome through specific transposition mechanisms. Furthermore, given its size, detailed annotation, and user-friendly implementation, ASTER-REP will be a useful platform to study transposon variability, helping to unravel the genome structure and improving transposon annotation for other plant genomes. [Extracted from the article]

Stevia rebaudiana is one of the most important crops belonging to the Asteraceae family. Stevia is cultivated all over the world as it represents a valid natural alternative to artificial sweeteners thanks to its leaves, which produce steviol glycosides that have high sweetening power and reduced caloric value. In this work, the stevia genome sequence was used to isolate and characterise full-length long-terminal repeat retrotransposons (LTR-REs), which account for more than half of the genome. The Gypsy retrotransposons were twice as abundant as the Copia ones. A disproportionate abundance of elements belonging to the Chromovirus/Tekay lineage was observed among the Gypsy elements. Only the SIRE and Angela lineages represented significant portions of the genome among the Copia elements. The dynamics with which LTR-REs colonised the stevia genome were also estimated; all isolated full-length elements turned out to be relatively young, with a proliferation peak around 1–2 million years ago. However, a different analysis conducted by comparing sequences encoding retrotranscriptase showed the occurrence of an older period in which there was a lot of LTR-RE proliferation. Finally, a group of isolated full-length elements belonging to the lineage Angela was used to analyse the genetic variability in 25 accessions of S. rebaudiana using the Inter-Retrotransposon Amplified Polymorphism (IRAP) protocol. The obtained fingerprints highlighted a high degree of genetic variability and were used to study the genomic structures of the different accessions. It was hypothesised that there are four ancestral subpopulations at the root of the analysed accessions, which all turned out to be admixed. Overall, these data may be useful for genome sequence annotations and for evaluating genetic variability in this species, which may be useful in stevia breeding. [ABSTRACT FROM AUTHOR]

In this paper, a pioneer partial discharge (PD) loop antenna sensor is presented and examined. It is made of a 70-turn square planar inductor with a side length of 1.8 mm, which is fabricated on top of a silicon substrate in complementary metal oxide semiconductor technology. The microsensor ability to detect corona PD is demonstrated once connected in series with a 60 dB gain amplifier. The behavior is studied at different separation distances from the line through which the PD pulses flow. At 5 cm away, a damped sinusoidal induced voltage with an amplitude of about 100 mV has been measured. The output signal spectrum is highly concentrated around a central resonance frequency of ∼5 MHz. The microsensor response is compared with those of other industrial sensors from Techimp, i.e., horn antennas and high-frequency current transformer sensors. The presented on-chip sensor can be considered a non-intrusive competing solution compared with other heavy and expensive commercial sensors due to its lightweight, compact size, and low cost. In addition, it shows an acceptable signal to noise ratio compared with other commercial electromagnetic wave-based sensors. [ABSTRACT FROM AUTHOR]

Background: Long Terminal Repeat retrotransposons (LTR-REs) are repetitive DNA sequences that constitute a large part of the genome. The improvement of sequencing technologies and sequence assembling strategies has achieved genome sequences with much greater reliability than those of the past, especially in relation to repetitive DNA sequences. Results: In this study, we analysed the genome of Ficus carica L., obtained using third generation sequencing technologies and recently released, to characterise the complete complement of full-length LTR-REs to study their dynamics during fig genome evolution. A total of 1867 full-length elements were identified. Those belonging to the Gypsy superfamily were the most abundant; among these, the Chromovirus/Tekay lineage was the most represented. For the Copia superfamily, Ale was the most abundant lineage. Measuring the estimated insertion time of each element showed that, on average, Ivana and Chromovirus/Tekay were the youngest lineages of Copia and Gypsy superfamilies, respectively. Most elements were inactive in transcription, both constitutively and in leaves of plants exposed to an abiotic stress, except for some elements, mostly belonging to the Copia/Ale lineage. A relationship between the inactivity of an element and inactivity of genes lying in close proximity to it was established. Conclusions: The data reported in this study provide one of the first sets of information on the genomic dynamics related to LTR-REs in a plant species with highly reliable genome sequence. Fig LTR-REs are highly heterogeneous in abundance and estimated insertion time, and only a few elements are transcriptionally active. In general, the data suggested a direct relationship between estimated insertion time and abundance of an element and an inverse relationship between insertion time (or abundance) and transcription, at least for Copia LTR-REs. [ABSTRACT FROM AUTHOR]

Summary: Due to DNA heterozygosity and repeat content, assembly of non‐model plant genomes is challenging. Herein, we report a high‐quality genome reference of one of the oldest known domesticated species, fig (Ficus carica L.), using Pacific Biosciences single‐molecule, real‐time sequencing. The fig genome is ~333 Mbp in size, of which 80% has been anchored to 13 chromosomes. Genome‐wide analysis of N6‐methyladenine and N4‐methylcytosine revealed high methylation levels in both genes and transposable elements, and a prevalence of methylated over non‐methylated genes. Furthermore, the characterization of N6‐methyladenine sites led to the identification of ANHGA, a species‐specific motif, which is prevalent for both genes and transposable elements. Finally, exploiting the contiguity of the 13 pseudomolecules, we identified 13 putative centromeric regions. The high‐quality reference genome and the characterization of methylation profiles, provides an important resource for both fig breeding and for fundamental research into the relationship between epigenetic changes and phenotype, using fig as a model species. Significance Statement: We report a significant improvement of the Ficus carica L. genome sequence and annotation. Furthermore, we performed a genome‐wide analysis of N6‐methyladenine and N4‐methylcytosine. The characterization of N6‐methyladenine sites led to the identification of ANHGA, a species‐specific motif. Finally, we identified the putative centromeric regions. The characterization of two unconventional DNA modifications provides an important resource for investigating the relationship between epigenetic changes and the phenotype of plants. [ABSTRACT FROM AUTHOR]

Electrical machines for transportation applications need to be highly reliable, particularly if they drive safety-critical systems. At the same time, another main requirement is represented by the significant torque density, especially for aerospace, where weight constraints are extremely stringent. For achieving high peak torque, an effective strategy consists in supplying the windings with a current greater than the rated value; thus, thermally overloading the machine for limited time periods. However, if the insulation is overheated, the machine lifetime is shortened and reliability issues can arise. This paper experimentally investigates the influence of short-time thermal overload on the insulation lifetime for low voltage, random wound electrical machines. The analysis is performed on round enameled magnet wire coils, which are aged by accelerated thermal cycles. The obtained results are statistically processed through a two parameter Weibull distribution. According to the findings of the experimental data postprocessing, a lifetime prediction model is built. This model is employed for predicting the lifetime consumption of a motor embedded into an electromechanical actuator for aerospace application. [ABSTRACT FROM AUTHOR]

The dynamics of long-terminal-repeat retrotransposons in two poplar species (Populus deltoides and P. nigra) and in an interspecific hybrid, recently synthesized, were investigated by analyzing the genomic abundance and transcription levels of a collection of 828 full-length retroelements identified in the genome sequence of P. trichocarpa , all occurring also in the genomes of P. deltoides and P. nigra. Overall, genomic abundance and transcription levels of many retrotransposons in the hybrid resulted higher or lower than expected by calculating the mean of the parental values. A bioinformatics procedure was established to ascertain the occurrence of the same retrotransposon loci in the three genotypes. The results indicated that retrotransposon abundance variations between the hybrid and the mean value of the parents were due to i) co-segregation of retrotransposon high- or low-abundant haplotypes; ii) new retroelement insertions; iii) retrotransposon loss. Concerning retrotransposon expression, this was generally low, with only 14/828 elements over- or under-expressed in the hybrid than expected by calculating the mean of the parents. It is concluded that interspecific hybridisation between the two poplar species determine quantitative variation and differential expression of some retrotransposons, with possible consequences for the genetic differentiation of the hybrid. • Retrotransposons were differently abundant in a hybrid compared to its parents. • Retrotransposons were differently expressed in the hybrid and the parents. • A pipeline to identify retrotransposon-related structural variations was established. • New retrotransposon insertion events were shown in the hybrid. • Data indicated different mechanisms producing structural variations. [ABSTRACT FROM AUTHOR]

Partial discharge inception voltage measurements under repetitive impulsive voltages play an essential role in evaluating the reliability of inverter-fed low voltage rotating machines. Using impulsive voltages, partial discharge (PD) signals and the disturbance induced by power electronics switches have often similar energy distribution in the frequency domain. Thus, PD test technologies used with sinusoidal voltages are no longer effective. Under repetitive impulsive voltages, ultra-high frequency detection is recommended to couple high-frequency spectral components from the PD and remove the low-frequency disturbance coming from power electronics. However, to obtain high signal to noise (S/N) ratio, the parameters of the antenna used for PD detection should be designed considering the PD and the disturbance energy distributions in the frequency domain. In this paper, we discuss the design and show the properties of an antenna with Archimedes plane spiral structure intended for PD detection under repetitive impulse voltages. The simulation shows that the antenna has low voltage standing wave ratio (<2) and improved directivity in the frequency range from 0.5 to 2 GHz. Moreover, PD tests performed using repetitive square waves with different rise times demonstrate that the designed antenna can receive PD signals efficiently with high S/N. [ABSTRACT FROM AUTHOR]