1,522 results on '"C., Esposito"'
Search Results
452. Air Pollution, Politics, and Legal Elitism
- Author
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John C. Esposito and Geoffrey Wandesforde-Smith
- Subjects
Politics ,Political economy ,Political science ,Air pollution ,medicine ,General Medicine ,medicine.disease_cause ,Law ,Elitism - Published
- 1971
453. Dkwecker Scissors
- Author
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Albert C. Esposito
- Subjects
Ophthalmology ,medicine.medical_specialty ,Device removal ,business.industry ,medicine.medical_treatment ,medicine ,Microsurgery ,business ,Surgery - Published
- 1958
454. RESOLUTION
- Author
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George M. Haik, Albert C. Esposito, and Alston Callahan
- Subjects
General Medicine - Published
- 1961
455. Cooperative Effect of GO and Glucose on PEDOT:PSS for High VOC and Hysteresis-Free Solution-Processed Perovskite Solar Cells.
- Author
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Giuri, A., Masi, S., Colella, S., Kovtun, A., Dell‐Elce, S., Treossi, E., Liscio, A., Corcione, C. Esposito, Rizzo, A., and Listorti, A.
- Subjects
LEAD iodide ,PEROVSKITE ,SOLAR cells - Abstract
A correction to the article "Cooperative Effect of GO and Glucose on PEDOT:PSS for High VOC and Hysteresis-Free Solution-Processed Perovskite Solar Cells" in the 2016 issue is presented.
- Published
- 2017
- Full Text
- View/download PDF
456. Advancing and Validating Models of Cognitive Architecture
- Author
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Carl Vogel, Anna Esposito, Vogel C, Esposito A, Vogel, C., and Esposito, A.
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Cognitive science ,03 medical and health sciences ,0302 clinical medicine ,Computer science ,Formal language ,0202 electrical engineering, electronic engineering, information engineering ,Robot ,020201 artificial intelligence & image processing ,Cognition ,02 engineering and technology ,Cognitive architecture ,Human behavior ,030217 neurology & neurosurgery - Abstract
We present a methodology for proposing and evaluating components of cognitive architectures comprising complementary approaches: hypothesizing and behavioral mechanisms, evaluating the aptness of those mechanisms in providing accounts of human behaviors; embed models of hypothesized mechanisms within simulation systems and observe synthesized model behaviors. We illustrate these theoretical approaches with examples.
- Published
- 2019
457. Linguistic and Behaviour Interaction Analysis within Cognitive Infocommunications
- Author
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Carl Vogel, Anna Esposito, Vogel C, Esposito A, Vogel, C., and Esposito, A.
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Cognitive science ,business.industry ,Cognition ,02 engineering and technology ,Clothing ,030226 pharmacology & pharmacy ,Visualization ,03 medical and health sciences ,InformationSystems_MODELSANDPRINCIPLES ,0302 clinical medicine ,Humanity ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,business ,Psychology ,Gesture - Abstract
Cognitive infocommunications is a discipline that examines the extensions of human cognitive capabilities that are assimilated within the concept of humanity. We argue that necessary (but not sufficient) conditions for the success of any candidate technology include solving problems within private and public spheres, in thought and communication. Exemplars such as emotion, gesture and language, as cognitive infocommunication technologies that have been assimilated, are examined. Implications for research programmes within cognitive infocommunications are addressed.
- Published
- 2019
458. New Anticancer Agents Mimicking Protein Recognition Motifs
- Author
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Michela Varra, Anna Ramunno, Silvia Franceschelli, Alfonso Carotenuto, Nausicaa Orteca, Diego Brancaccio, Luigi Michele Pavone, Valeria De Pasquale, Vita Maglio, Ettore Novellino, Caterina Fattorusso, Chiara Esposito, Marco Persico, Persico, Marco, A., Ramunno, V., Maglio, S., Franceschelli, C., Esposito, Carotenuto, Alfonso, Brancaccio, Diego, DE PASQUALE, Valeria, Pavone, LUIGI MICHELE, Varra, Michela, Orteca, Nausicaa, Novellino, Ettore, and Fattorusso, Caterina
- Subjects
Models, Molecular ,Magnetic Resonance Spectroscopy ,drug design ,Stereochemistry ,Antineoplastic Agents ,Apoptosis ,protein-protein interaction ,Structure-Activity Relationship ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,Protein recognition ,Side chain ,Humans ,Solubility ,P53 expression ,Pyrrole ,Cell Cycle ,Molecular Mimicry ,Proteins ,Biological activity ,peptidomimetic ,Nuclear translocation ,anticancer agent ,chemistry ,Molecular Medicine ,Pharmacophore - Abstract
The novel tetrasubstituted pyrrole derivatives 8g, 8h, and 8i showed selective cytotoxicity against M14 melanoma cells at low micromolar concentration. Structure???activity relationships (SARs) indicated the presence of three aromatic substituents on the pyrrole core as necessary for biological activity. Computational studies strongly suggest that the peculiar 3D orientation of these substituents is able to reproduce the hydrophobic side chains in LxxLL-like protein recognition motifs. Biological results showed altered p53 expression and nuclear translocation in cells sensitive to the compounds, suggesting p53 involvement in their anticancer mechanism of action. Unfortunately, because of poor solubility of the active analogues, it was not possible to perform further investigation by NMR techniques. Pharmacophore models were generated and used to perform 3D searches in molecular databases. Results indicated that two compounds share the same pharmacological profile and the same pharmacophoric features with our new derivatives, and one of them inhibited MDM2???MDM4 heterodimer formation.
- Published
- 2013
459. Un’aggiunta a Saverio Gatto
- Author
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PICONE, MARIANTONIETTA, Valente I., Romano d'Orsi, R, Genovese C., Esposito D., Ruotolo R., et alii, Valente, I., and Picone, Mariantonietta
- Subjects
Saverio Gatto ,Adolfo Wildt ,Scultura del Novecento ,Scultura napoletana del Novecento - Abstract
Volume speciale dedicato alla mostra Il Bello o il Vero. La scultura napoletana del secondo Ottocento e del primo Novecento, Napoli, Complesso monumentale di San Domenico Maggiore, 30 ottobre 2014-6 giugno 2015. Il saggio su Saverio Gatto esamina in particolare due opere che sono state aggiunte alla mostra in un secondo momento e che sono poi state donate alle collezioni del Comune di Napoli. A partire da queste opere si parla in particolare del rapporto che c'è stato fra Saverio Gatto e Adolfo Wildt, rapporto testimoniato, oltre che da alcuni elementi di linguaggio scultorio comuni, anche dall'esistenza di alcune lettere di Wildt a Gatto che qui si pubblicano integralmente per la prima volta.
- Published
- 2015
460. Giovanni Tizzano attraverso alcuni giudizi della critica
- Author
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PICONE, MARIANTONIETTA, Valente I., Romano d'Orsi, R, Genovese C., Esposito D., Ruotolo R., et alii, Valente I., and Picone, Mariantonietta
- Subjects
Vincenzo Costantini ,Scultura del Novecento ,Scultura napoletana del Novecento ,Paolo Ricci ,Giovanni Tizzano ,Claude Mattey - Abstract
In questo volume, che va a completare il catalogo della mostra Il Bello o il vero, curata da Isabella Valente, il saggio prende in esame la figura di Giovanni Tizzano in chiave di storia della critica. Lo spunto è nato dalla donazione di una ventina di opere dello scultore alle collezioni del Comune di Napoli e ora esposte nel Museo Civico di Castel Nuovo a Napoli, dopo essere state esposte nella mostra Il Bello o il Vero. Il saggio cerca di mettere in evidenza una serie di cliché presenti nella critica d'arte coeva dell'artista accanto ad alcuni elementi di giudizio ancora oggi condivisibili. Il saggio è corredato da un ampio repertorio di immagini delle sculture, individuando con precisione quali opere erano presenti nelle numerose mostre del suo percorso artistico.
- Published
- 2015
461. Somma-Vesuvius’s activity over the last 33 ka years: a mineral chemistry prospective
- Author
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Redi, Daniele, Danyushevsky, Leonid, Esposito, Rosario, LIMA, ANNAMARIA, CANNATELLI, CLAUDIA, DE VIVO, BENEDETTO, REDI D., DANYUSHEVSKY L., LIMA A., CANNATELLI C., ESPOSITO R., DE VIVO B., Redi, Daniele, Danyushevsky, Leonid, Lima, Annamaria, Cannatelli, Claudia, Esposito, Rosario, and DE VIVO, Benedetto
- Published
- 2015
462. N-terminus end of rat prostate transglutaminase is responsible for its catalytic activity and GTP binding
- Author
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Loredana Mariniello, Ivana Caputo, Angela Sorrentino, Raffaele Porta, Carla Esposito, Mariniello, Loredana, C., Esposito, I., Caputo, Sorrentino, Angela, and Porta, Raffaele
- Subjects
Male ,rat prostate ,DNA, Complementary ,GTP' ,Tissue transglutaminase ,type 4 transglutaminase ,Immunoblotting ,Biology ,Biochemistry ,law.invention ,Dogs ,GTP-Binding Proteins ,Prostate ,law ,Catalytic Domain ,Complementary DNA ,medicine ,Animals ,Secretion ,Cells, Cultured ,chemistry.chemical_classification ,Transglutaminases ,Cell Biology ,Recombinant Proteins ,Rats ,N-terminus ,medicine.anatomical_structure ,Enzyme ,chemistry ,biology.protein ,Recombinant DNA - Abstract
Rat prostate transglutaminase is characterized by a high degree of complexity. In fact, as previously demonstrated, it is highly glycosilated and possesses a lipid anchor which is retained during enzyme apocrine secretion. In order to assess the importance of such modifications upon enzyme functionality, full length rat prostate transglutaminase cDNA has been synthesized by RT-PCR and stably expressed in MDCK cells. The recombinant form has been partially purified by GTP-affinity chromatography, a technique which has been used to purify the enzyme produced from rat prostate secretion. The recombinant protein is endowed with enzymatic activity even though, as we have demonstrated by immunological studies, it lacks post-translational modifications which occur in the prostate enzyme. Moreover, we have demonstrated that a deletion mutant, which gives rise to a protein lacking 103 amino acid residues at the N-terminus end, loses enzymatic activity and the capability of binding GTP. This study shows that, while post-translational modifications are not essential for enzymatic activity, the N-terminus end is responsible for both transglutaminase functionality and GTP-binding. (C) 2003 Elsevier Science Ltd. All rights reserved.
- Published
- 2003
463. Structural characterization of transglutaminase-catalyzed cross-linking between glyceraldehyde 3-phosphate dehydrogenase and polyglutamine repeats
- Author
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Simona Francese, Ivana Caputo, Margherita Ruoppolo, Carla Esposito, Stefania Orrù, Ruoppolo, Margherita, S., Orrù, S., Francese, I., Caputo, and C., Esposito
- Subjects
Tissue transglutaminase ,Guinea Pigs ,Lysine ,Protein aggregation ,Biochemistry ,Article ,Catalysis ,Substrate Specificity ,GTP-binding protein regulators ,Trinucleotide Repeats ,GTP-Binding Proteins ,Animals ,Protein Glutamine gamma Glutamyltransferase 2 ,Glycolysis ,Molecular Biology ,Glyceraldehyde 3-phosphate dehydrogenase ,chemistry.chemical_classification ,Transglutaminases ,biology ,Chemistry ,Glyceraldehyde-3-Phosphate Dehydrogenases ,Neurodegenerative Diseases ,Cytosol ,Enzyme ,Liver ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Rabbits ,Peptides - Abstract
The accumulation of abnormal polyglutamine-containing protein aggregates within the cytosol and nuclei of affected neurons is a hallmark of the progressive neurodegenerative disorders caused by an elongated (CAG)(n) repeat in the genome. The polyglutamine domains are excellent substrates for the enzyme transglutaminase type 2 (tissue), resulting in the formation of cross-links with polypeptides containing lysyl groups. Enzymatic activity toward the Q(n) domains increases greatly upon lengthening of such Q(n) stretches (n40). Among the possible amine donors, the glycolytic enzyme glyceraldehyde-3-phosphate-dehydrogenase was shown to tightly bind several proteins involved in polyglutamine expansion diseases. Recently, the authors have shown that K191, K268, and K331, out of the 26 lysines present in glyceraldehyde-3-phosphate-dehydrogenase, are the reactive amine-donor sites forming cross-links with substance P, which bears the simplest Q(n) domain (n = 2). The present study reports that synthetic peptides of both pathological and nonpathological length (n = 43 and 17, respectively) form cross-links with the same K residues located in the C-terminal region of glyceraldehyde-3-phosphate-dehydrogenase. In addition, it is shown that extra K residues present in the C termini of glyceraldehyde-3-phosphate-dehydrogenase are susceptible to cross-linking in the presence of transglutaminase. The present results indicate a possible modulating effect of Q(n) stretches on tissue transglutaminase substrate specificity and mechanism of recognition.
- Published
- 2003
464. Neurokinin Receptors Could Be Differentiated by Their Capacity to Respond to the Transglutaminase-Synthesized γ-(Glutamyl5)Spermine Derivative of Substance P
- Author
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P. Di Pierro, Antonio Calignano, F. Mancuso, Carla Esposito, Raffaele Porta, Piero Pucci, C., Esposito, F., Mancuso, Calignano, Antonio, DI PIERRO, Prospero, Pucci, Pietro, and Porta, Raffaele
- Subjects
Male ,Agonist ,Chemical Phenomena ,Platelet Aggregation ,medicine.drug_class ,Tissue transglutaminase ,Guinea Pigs ,Spermine ,Blood Pressure ,Substance P ,Biochemistry ,Nitric oxide ,Guinea pig ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Ileum ,medicine ,Animals ,Receptor ,Aorta ,Receptors, Tachykinin ,Inflammation ,Transglutaminases ,biology ,fungi ,Hindlimb ,Rats ,Vasodilation ,Spermidine ,Chemistry ,chemistry ,biology.protein ,Collagen ,Rabbits ,Platelet Aggregation Inhibitors - Abstract
Four different gamma-(glutamyl(5))amine derivatives of substance P (SP) were synthesized in vitro in the presence of purified guinea pig liver transglutaminase and Ca2+. The 1,3-diaminopropane, spermidine, spermine (Spm), and monodansylcadaverine adducts of the neuropeptide were purified by HPLC on a reversed-phase column and characterized by fast atom bombardment mass spectrometry. The gamma-(glutamyl(5))Spm derivative of SP (Spm-SP) was found to be able, like the parent neuropeptide, to provoke rabbit aorta relaxation, to decrease rat arterial blood pressure, and to inhibit collagen-induced platelet aggregation. Unlike SP, only a weak inflammatory response was observed when Spm-SP was injected in the rat hind limb. All these effects were found to be prevented by N-omega-nitro-L-arginine methyl ester, a well-known nitric oxide synthesis inhibitor. In contrast, Spm-SP was completely ineffective in contracting guinea pig ileal segments, thus confirming our preliminary observations indicating that Spm-SP does not evoke SP-like spasmogenic effects on isolated smooth muscle preparations. The specificity of the effects due to the selective introduction of a Spm moiety al the glutamine(5) level was demonstrated by the SP agonist pharmacological profile of the other gamma-(glutamyl(5))amine derivatives tested. These results suggest that neurokinin receptors could be differentiated by their capacity to respond to Spm-SP.
- Published
- 2002
465. Rat Coagulating Gland Secretion Contains a Kinesin Heavy Chain-like Protein Acting as a Type IV Transglutaminase Substrate
- Author
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Stefania Orrù, Carla Esposito, Angela Amoresano, Raffaele Porta, Loredana Mariniello, Anna Cozzolino, C., Esposito, Mariniello, Loredana, A., Cozzolino, Amoresano, Angela, S., Orru, and Porta, Raffaele
- Subjects
Male ,Spermidine ,Tissue transglutaminase ,Blotting, Western ,Molecular Sequence Data ,Kinesins ,Biochemistry ,Substrate Specificity ,Mice ,HSPA2 ,Animals ,Secretion ,Amino Acid Sequence ,Carbon Radioisotopes ,Rats, Wistar ,Gene ,chemistry.chemical_classification ,Transglutaminases ,Sequence Homology, Amino Acid ,biology ,Molecular Motor Proteins ,Seminal Plasma Proteins ,Proteins ,Seminal Vesicles ,In vitro ,Rats ,Amino acid ,Enzyme ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Kinesin - Abstract
By a proteomic approach, we demonstrated in rat coagulating gland secretion the presence of a 120 kDa protein which shares at least 80% identity at the amino acid level with the most closely related kinesin heavy chain codified by the kinesin superfamily protein Kif5c gene. In addition, we identified 30 and 66 kDa proteolytic fragments of such a kinesin heavy chain-like protein, corresponding to the 73- 299 N-terminal and 300-860 C-terminal regions, respectively. Finally, we demonstrated the occurrence in coagulating gland secretion of a 200 kDa protein probably derived by cross-linking reaction of the kinesin heavy chain-like protein with type IV transglutaminase. In fact, kinesin heavy chain-like protein and its 66 kDa proteolytic fragment were also found to act as effective acyl donor substrates for the enzyme in vitro.
- Published
- 2001
466. Enteropathic Spondyloarthritis: From Diagnosis to Treatment
- Author
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Carmela Esposito, Raffaele Scarpa, Pasquale Ambrosino, Rosario Peluso, Fabiana Castiglione, Antonella Scalera, Matteo Nicola Dario Di Minno, Salvatore Iervolino, Francesco Manguso, Giuseppina Tramontano, Peluso, Rosario, M. N., Dario, S., Iervolino, F., Manguso, G., Tramontano, P., Ambrosino, C., Esposito, A., Scalera, Castiglione, Fabiana, R., Scarpa, DI MINNO, Matteo, Iervolino, S, Manguso, F, Tramontano, G, Ambrosino, P, Esposito, C, Scalera, A, and Scarpa, Raffaele
- Subjects
musculoskeletal diseases ,lcsh:Immunologic diseases. Allergy ,medicine.medical_specialty ,Spondyloarthropathy ,Immunology ,Anti-Inflammatory Agents ,Arthritis ,Gene Expression ,Physical examination ,Inflammation ,Review Article ,Gastroenterology ,Pathogenesis ,Internal medicine ,Fibromyalgia ,Spondylarthritis ,medicine ,Immunology and Allergy ,Humans ,Medical history ,Genetic Predisposition to Disease ,Intestinal Mucosa ,HLA-B27 Antigen ,medicine.diagnostic_test ,business.industry ,General Medicine ,medicine.disease ,Inflammatory Bowel Diseases ,Dermatology ,Gastrointestinal Tract ,Rheumatoid arthritis ,Joints ,medicine.symptom ,business ,lcsh:RC581-607 - Abstract
Enteropathic arthritis (EA) is a spondyloarthritis (SpA) which occurs in patients with inflammatory bowel diseases (IBDs) and other gastrointestinal diseases. Diagnosis is generally established on the medical history and physical examination. It was, generally, made according to the European Spondyloarthropathy Study Group (ESSG) criteria. Rheumatic manifestations are the most frequent extraintestinal findings of IBD with a prevalence between 17% and 39%, and IBD is associated, less frequently, with other rheumatic disease such as rheumatoid arthritis, Sjogren syndrome, Takayasu arteritis, and fibromyalgia. Although the pathogenesis of EA has not been plainly clarified, the most popular theory supposes that joint inflammation occurs in genetically predisposed subjects with bacterial gut infections, provided an important evidence for a possible relationship between inflammation of the gut mucosa and arthritis. The management of patients with EA requires an active cooperation between the gastroenterologist and rheumatologist.
- Published
- 2013
467. Are Acantholysis and Transglutaminase Inhibition Related Phenomena?
- Author
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A. Lo Schiavo, Anna Cozzolino, Vincenzo Ruocco, Raffaele Porta, Maria Luisa Lombardi, Cinzia Esposito, C., Esposito, V., Ruocco, A., Cozzolino, A., Lo Schiavo, M. L., Lombardi, Porta, Raffaele, Esposito, C, Ruocco, Vincenzo, Cozzolino, A, LO SCHIAVO, Ada, Lombardi, Ml, and Porta, R.
- Subjects
Keratinocytes ,Captopril ,Biochemical Phenomena ,Tissue transglutaminase ,Cystamine ,Angiotensin-Converting Enzyme Inhibitors ,Human skin ,Dermatology ,Binding, Competitive ,Biochemistry ,chemistry.chemical_compound ,Enalapril ,Cell Adhesion ,medicine ,Humans ,Breast ,Disulfides ,Sulfhydryl Compounds ,Enzyme Inhibitors ,Cells, Cultured ,Skin ,Transglutaminases ,integumentary system ,biology ,Acantholysis ,Tiopronin ,medicine.disease ,In vitro ,Pemphigus ,medicine.anatomical_structure ,chemistry ,Enzyme inhibitor ,biology.protein ,Keratinocyte - Abstract
Background: The loss of intercellular cohesion among keratinocytes (acantholysis) may be considered the histologic marker of pemphigus. Many drugs, especially thiol drugs, proved to be able to provoke in vitro acantholysis by biochemical mechanisms interfering with the disulfide and thiol group balance. As to nonthiol drugs, the pathomechanism of acantholysis is still unexplained. Objective: To explain the molecular mechanism of enalapril-induced acantholysis a potential link between transglutaminase (TGase) activity and the effects of this drug was investigated. Methods: TGase activity in extracts from human breast skin cultured in the presence of thiopronine, captopril and enalapril were evaluated in vitro. The acantholytic potential of cystamine, a known TGase inhibitor, was also investigated. Results: Enalapril, the most powerful acantholytic drug in vitro, was found to inhibit both the purified enzyme and the TGase activity in the extracts from cultured human breast skin explants. Kinetic studies showed that enalapril inhibition was competitive with respect to the amino acceptor substrate and uncompetitive with respect to the amino donor substrate. Moreover, an acantholytic effect of cystamine on explants of normal human skin was shown. Conclusions: These results suggest that acantholysis and the inhibition of TGase activity could be two related phenomena.
- Published
- 1996
468. The side chain of glutamine 13 is the acyl-donor amino acid modified by type 2 transglutaminase in subunit T of the native rabbit skeletal muscle troponin complex
- Author
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Stefano Annunziata, Carlo M. Bergamini, Carlo Cervellati, Rita Casadio, Piero Pucci, Blendi Ura, Carlo Mischiati, Carla Iannone, Monica Squerzanti, Carla Esposito, Squerzanti M, Cervellati C, Ura B, Mischiati C, Pucci P, Annunziata S, Iannone C, Casadio R, Bergamini CM, Esposito C, M., Squerzanti, C., Cervellati, B., Ura, C., Mischiati, Pucci, Pietro, S., Annunziata, C., Iannone, R., Casadio, C. M., Bergamini, and C., Esposito
- Subjects
PROTEIN POST-TRANSLATIONAL MODIFICATION ,Erythrocytes ,Protein post-translational modification ,Skeletal troponin T ,Transglutaminase ,Amino Acid Sequence ,Animals ,Calcium ,Conserved Sequence ,GTP-Binding Proteins ,Glutamine ,Humans ,Molecular Sequence Data ,Muscle, Skeletal ,Protein Processing, Post-Translational ,Protein Subunits ,Rabbits ,Sequence Analysis, Protein ,Spermine ,Transglutaminases ,Troponin T ,Tissue transglutaminase ,Protein subunit ,Clinical Biochemistry ,macromolecular substances ,Biochemistry ,Troponin complex ,medicine ,Protein Glutamine gamma Glutamyltransferase 2 ,Protein Processing ,biology ,Chemistry ,Protein ,Organic Chemistry ,Cardiac muscle ,Post-Translational ,Skeletal muscle ,Skeletal ,Troponin ,Molecular biology ,medicine.anatomical_structure ,SKELETAL TROPONIN T ,TRANSGLUTAMINASE ,biology.protein ,Muscle ,Sequence Analysis - Abstract
Subunit T of the native muscle troponin complex is a recognised substrate of transglutaminase both in vitro and in situ with formation of isopeptide bonds. Using a proteomic approach, we have now determined the precise site of in vitro labelling of the protein. A preparation of troponin purified from ether powder from mixed rabbit skeletal muscles was employed as transglutaminase substrate. The only isoform TnT2F present in our preparation was recognised as acyl-substrate by human type 2 transglutaminase which specifically modified glutamine 13 in the N-terminal region. During the reaction, the troponin protein complex was polymerized. Results are discussed in relation to the structure of the troponin T subunit, in the light of the role of troponins in skeletal and cardiac muscle diseases, and to the rules governing glutamine side chain selection by tissue transglutaminase.
- Published
- 2011
469. L'artista e il costruttore scenico
- Author
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FIORILLO, CLARA, A. Dentale e C. Esposito, and Fiorillo, Clara
- Subjects
Arte Scenografia - Published
- 2011
470. IL PARADOSSO DELL'AVANGUARDIA
- Author
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CARAMIELLO, LUIGI, A. DENTALE, C. ESPOSITO, and Caramiello, Luigi
- Subjects
AVANGUARDIE ARTISTICHE ,PARADOSSO ,SOCIOLOGIA - Published
- 2011
471. Dynamical properties of cold shock protein A from Mycobacterium tuberculosis
- Author
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Gabriella D'Auria, Carla Esposito, Rita Berisio, Emilia Pedone, Luisa Calvanese, Lucia Falcigno, Alessia Ruggiero, Emanuela Iaccarino, Carlo Pedone, D'Auria, Gabriella, C., Esposito, Falcigno, Lucia, L., Calvanese, E., Iaccarino, A., Ruggiero, C., Pedone, E., Pedone, and R., Berisio
- Subjects
Circular dichroism ,Protein Folding ,Protein Conformation ,Bs CspB ,unfolding multiple pathways ,Molecular Sequence Data ,Biophysics ,high temperature MD simulation ,Bacillus subtilis ,Antiparallel (biochemistry) ,EMSA ,Biochemistry ,Protein Structure, Secondary ,Protein structure ,ss DNA binding ,Bacterial Proteins ,MTB CspA ,Amino Acid Sequence ,Molecular Biology ,Nuclear Magnetic Resonance, Biomolecular ,biology ,Oligonucleotide ,Circular Dichroism ,Cell Biology ,Mycobacterium tuberculosis ,Cold-shock domain ,biology.organism_classification ,Nucleic acid ,Protein folding - Abstract
Bacterial cold shock proteins (Csps) are over-expressed as response to cold stress. They have a role in transcriptional and translational events due to their ability to bind single stranded (ss) nucleic acids. Csps so far characterized show similar structures with a closed five stranded antiparallel beta-barrel. Here we report a structural and functional study of cold shock protein A from Mycobacterium tuberculosis, MTBCspA. Structural investigations by CD and NMR reveal that MTB-CspA is less ordered than expected and is the least thermal stable cold shock protein so far characterized. However, electrophoretic mobility shift assays show that MTB-CspA is functionally active, as it is able to bind oligonucleotides. The dynamic behavior of MTB-CspA, compared to its homolog from Bacillus subtilis, was investigated by molecular dynamics simulations at room and high temperatures. Analysis of trajectories point to specific regions on beta1 and beta4 strands, likely responsible for the higher structural fragility of MTB-CspA. Also, they show that the nucleic-acid binding region of MTB-CspA is the least prone to unfolding, a finding which explains the ability of MTB-CspA to exert its function. 2010 Elsevier Inc. All rights reserved.
- Published
- 2010
472. Stati intersessuali
- Author
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LIMA, MARIO, RUGGERI, GIOVANNI, C. Antonellini, S. Pavia, M. Mogiatti, C. ESPOSITO ET AL., M.Lima, G.Ruggeri, C.Antonellini, S.Pavia, and M.Mogiatti
- Subjects
stati intersessuali - Abstract
I disordini della differenziazione sessuale costituiscono un argomento molto delicato e complesso. Il trattamento non può prescindere da un’accurata valutazione preoperatoria che permette il corretto inquadramento del paziente. La laparoscopia, utilizzata anche in questa fase iniziale, riveste un ruolo cardine con finalità sia diagnostiche sia terapeutiche. Nel capitolo vengono descritte le note tecniche della procedura.
- Published
- 2010
473. Prelievo di tessuto ovarico
- Author
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Giovanni Ruggeri, Mario Lima, Marcello Domini, V. Carlini, S. Pavia, C. ESPOSITO C. HOLLANDS M. LIMA A. SETTIMI J-S. VALLA, Lima, M., Ruggeri, G., Domini, M., Pavia, S., and Carlini, V.
- Subjects
criopreservazione, ovaio - Abstract
È stato dimostrato che l’impiego di ciclofosfamide e busulfano induce perdita di funzione ovarica nel 70% delle pazienti, mentre l’utilizzo di chemioterapia ad alte dosi e radioterapia total-body porta a infertilità il 92% delle giovani donne. Queste problematiche hanno diretto la ricerca verso tecniche che potessero tentare di preservare la fertilità futura di queste giovani pazienti. Tra queste, la crioconservazione degli embrioni è certamente la più diffusa, tuttavia necessita della presenza di una coppia stabile, di pazienti pubere e maggiorenni e dunque non è in concreto mai praticabile in età pediatrica. Un’alternativa relativamente recente è la crioconservazione del tessuto ovarico, che viene prelevato prima dell’inizio della terapia e congelato in attesa della completa remissione della malattia
- Published
- 2010
474. Ostruzioni intestinali
- Author
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M. Lima, G. Ruggeri, M. Libri, B. Randi, D. Beghelli, C. ESPOSITO ET AL., M.Lima, G.Ruggeri, M.Libri, B.Randi, and D.Beghelli
- Subjects
ostruzione intestinale - Abstract
Le atresie del piccolo intestino sono la causa più frequente di occlusione intestinale in età neonatale. Nel capitolo vengono presi in considerazione i vantaggi del trattamento laparoassitito di tali patologie che può essere considerato come un’evoluzione del trattamento laparoscopico puro. Vengono fornite note tecniche e vengono illustrate le complicanze e le controindicazioni a tale trattamento.
- Published
- 2010
475. Anti-transglutaminase antibodies in non-coeliac children suffering from infectious diseases
- Author
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N Di Toro, Stefano Martelossi, Alessandro Ventura, Serena Pastore, S Quaglia, Tarcisio Not, Fortunato Ferrara, Rita Giorgi, G. Dal Molin, Ivana Caputo, Marilena Lepretti, Carla Esposito, Fortunato, Ferrara, Sara, Quaglia, I., Caputo, C., Esposito, M., Lepretti, Pastore, Serena, R., Giorgi, Stefano, Martelossi, G., Dal Molin, Nicola Di, Toro, Ventura, Alessandro, and Not, Tarcisio
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Male ,Adolescent ,Translational Studies ,Immunology ,Enzyme-Linked Immunosorbent Assay ,infectious diseases ,Communicable Diseases ,Antibodies ,Coeliac disease ,Serology ,medicine ,Humans ,Immunology and Allergy ,Avidity ,Prospective Studies ,celiac disease ,Child ,Cytoskeleton ,Autoantibodies ,Transglutaminases ,biology ,Cell Cycle ,Panel reactive antibody ,Autoantibody ,nutritional and metabolic diseases ,medicine.disease ,Actins ,Microscopy, Fluorescence ,Infectious disease (medical specialty) ,Child, Preschool ,Anti-transglutaminase antibodies ,biology.protein ,Female ,Caco-2 Cells ,Antibody - Abstract
Summary Anti-transglutaminase antibodies are the diagnostic markers of coeliac disease. A role is suggested for infectious agents in the production of anti-transglutaminase antibodies. The aim was to measure positive anti-transglutaminase antibody levels in children with infectious diseases and to compare immunological and biological characteristics of the anti-transglutaminase antibodies derived from these children with that from coeliac patients. Two hundred and twenty-two children suffering from infectious diseases were enrolled prospectively along with seven biopsy-proven coeliacs. Serum samples were tested for anti-transglutaminase antibodies and anti-endomysium antibodies; positive samples were tested for coeliac-related human leucocyte antigen (HLA)-DQ2/8 and anti-viral antibodies. Purified anti-transglutaminase antibodies from the two study groups were tested for urea-dependent avidity, and their ability to induce cytoskeletal rearrangement and to modulate cell-cycle in Caco-2 cells, using phalloidin staining and bromodeoxyuridine incorporation assays, respectively. Nine of 222 children (4%) tested positive to anti-transglutaminase, one of whom also tested positive for anti-endomysium antibodies. This patient was positive for HLA-DQ2 and was diagnosed as coeliac following intestinal biopsy. Of the eight remaining children, two were positive for HLA-DQ8. Levels of anti-transglutaminase returned to normal in all subjects, despite a gluten-containing diet. Purified anti-transglutaminase of the two study groups induced actin rearrangements and cell-cycle progression. During an infectious disease, anti-transglutaminase antibodies can be produced temporarily and independently of gluten. The infection-triggered anti-transglutaminase antibodies have the same biological properties as that of the coeliacs, with the same in-vivo potential for damage.
- Published
- 2010
476. Membrane charge dependent states of the beta-amyloid fragment Abeta (16-35) with differently charged micelle aggregates
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Anna Ramunno, Anna Maria D'Ursi, Vittorio Limongelli, Ettore Novellino, Manuela Grimaldi, Cinzia Esposito, Mario Scrima, Gerardino D'Errico, Giuseppe Vitiello, M., Grimaldi, M., Scrima, C., Esposito, Vitiello, Giuseppe, A., Ramunno, Limongelli, Vittorio, D'Errico, Gerardino, Novellino, Ettore, and A. M., D'Ursi
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Circular dichroism ,Amyloid peptide ,spectroscopy ,Magnetic Resonance Spectroscopy ,Protein Conformation ,Molecular Sequence Data ,Static Electricity ,Biophysics ,Peptide ,Micelle ,Biochemistry ,Protein structure ,NMR spectroscopy ,Static electricity ,micelle ,Organic chemistry ,Computer Simulation ,Amino Acid Sequence ,Spin-labeled peptide ,Spin label ,Protein Structure, Quaternary ,Micelles ,chemistry.chemical_classification ,Amyloid beta-Peptides ,Circular Dichroism ,Cell Membrane ,Electron Spin Resonance Spectroscopy ,amyloid ,Cell Biology ,Random coil ,Peptide Fragments ,Membrane ,chemistry ,Micelle solution ,Indicators and Reagents ,Spin Labels ,EPR spectroscopy - Abstract
Abeta (16-35) is the hydrophobic central core of beta-amyloid peptide, the main component of plaques found in the brain tissue of Alzheimer's disease patients. Depending on the conditions present, beta-amyloid peptides undergo a conformational transition from random coil or alpha-helical monomers, to highly toxic beta-sheet oligomers and aggregate fibrils. The behavior of beta-amyloid peptide at plasma membrane level has been extensively investigated, and membrane charge has been proved to be a key factor modulating its conformational properties. In the present work we probed the conformational behavior of Abeta (16-35) in response to negative charge modifications of the micelle surface. CD and NMR conformational analyses were performed in negatively charged pure SDS micelles and in zwitterionic DPC micelles "doped" with small amounts of SDS. To analyze the tendency of Abeta (16-35) to interact with these micellar systems, we performed EPR experiments on three spin-labeled analogues of Abeta (16-35), bearing the methyl 3-(2,2,5,5-tetramethyl-1-oxypyrrolinyl) methanethiolsulfonate spin label at the N-terminus, in the middle of the sequence and at the C-terminus, respectively. Our conformational data show that, by varying the negative charge of the membrane, Abeta (16-35) undergoes a conformational transition from a soluble helical-kink-helical structure, to a U-turn shaped conformation that resembles protofibril models.
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- 2009
477. Five-year follow-up of children with perinatal HIV-1 infection receiving early highly active antiretroviral therapy
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Author:, Chiappini, Galli, E, Tovo, L, Gabiano, Pa, Lisi, C, Bernardi, C, Vigano, S, Guarino, A, Giaquinto, A, Esposito, C, Badolato, S, R, Bari, Di, Rosso, C, Genovese, R, Masi, O, Mazza, M, A, Martino, De, Italian Register for HIV Infection Other Partecipants: Osimani, M., Cordiali, P, Mattia, De, Manzinonna, D, M, Ruggeri, C, Masi, M, Miniaci, M, Specchia, A, Ciccia, F, Lanari, M, Baldi, M, Battisti, F, Bertulli, L, Dessì, C, Pintor, C, Dedoni, C, Fenu, M, Cavallini, Ml, Anastasio, R, Magnolia, E, Sticca, Mg, Pomero, M, Bezzi, G, Fiumana, T, Bonsignori, E, F, Gaudio, De, Gervaso, M, Cecchi, P, Viscoli, Mt, Cosso, C, Timitilli, D, Stronati, A, Plebani, M, Semino, A, Tei, M, Giacomet, F, Pivetti, V, Salvini, V, Zuccotti, F, Giovannini, Gv, Ferraris, M, Liprieri, G, Moretti, R, Cellini, C, Cano, M, Paolucci, Mc, Bruzzese, P, Giannattasio, E, Tarallo, A, Tancredi, L, Pennazzato, F, Rampon, M, O, Dalle, Nogare, Sanfilippo, Er, Romano, A, Saitta, M, Dodi, I, Bandello, M, Maccabruni, A, Felici, L, Consolini, Rita, Chiappini E., Galli L., Tovo PA., Gabiano C., Lisi C., Bernardi S., Viganò A., Guarino A., Giaquinto C., Esposito S., Badolato R., Di Bari C., Rosso R., Genovese O., Masi M., Mazza A., de Martino M., Specchia F., Molesini M., Chiappini, E., Galli, L., Tovo, P. -A., Gabiano, C., Lisi, C., Bernardi, S., Vigano, A., Guarino, A., Giaquinto, C., Esposito, S., Badolato, R., Di Bari, C., Rosso, R., Genovese, O., Masi, M., Mazza, A., and De Martino, M.
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medicine.medical_specialty ,Pediatrics ,Anti-HIV Agents ,HIV Infections ,Follow-Up Studie ,lcsh:Infectious and parasitic diseases ,Pharmacotherapy ,Medical microbiology ,Interquartile range ,Antiretroviral Therapy, Highly Active ,medicine ,Humans ,HIV Infection ,lcsh:RC109-216 ,Child ,Pregnancy ,business.industry ,Anti-HIV Agent ,Infant ,Viral Load ,medicine.disease ,Antiretroviral therapy ,hiv children ,CD4 Lymphocyte Count ,Regimen ,Infectious Diseases ,Italy ,Child, Preschool ,Tropical medicine ,Immunology ,HIV-1 ,RNA, Viral ,business ,Viral load ,Research Article ,Follow-Up Studies ,Human - Abstract
Background Early highly active antiretroviral therapy (HAART), started within the first months of age, has been proven to be the optimal strategy to prevent immunological and clinical deterioration in perinatally HIV-infected children. Nevertheless, data about long-term follow-up of early treated children are lacking. Methods We report data from 40 perinatally HIV-infected-children receiving early HAART, with a median follow-up period of 5.96 years (interquartile range [IQR]:4.21–7.62). Children were enrolled at birth in the Italian Register for HIV Infection in Children. Comparison with 91 infected children born in the same period, followed-up from birth, and receiving deferred treatment was also provided. Results Nineteen children (47.5%) were still receiving their first HAART regimen at last follow-up. In the remaining children the first regimen was discontinued, after a median period of 3.77 years (IQR: 1.71–5.71) because of viral failure (8 cases), liver toxicity (1 case), structured therapy interruption (3 cases), or simplification/switch to a PI-sparing regimen (9 cases). Thirty-nine (97.5%) children showed CD4+ T-lymphocyte values>25%, and undetectable viral load was reached in 31 (77.5%) children at last visit. Early treated children displayed significantly lower viral load than not-early treated children, until 6 years of age, and higher median CD4+ T-lymphocyte percentages until 4 years of age. Twenty-seven (29.7%) not-early treated vs. 0/40 early treated children were in clinical category C at last follow-up (P < 0.0001). Conclusion Our findings suggest that clinical, virologic and immunological advantages from early-HAART are long-lasting. Recommendations indicating the long-term management of early treated children are needed.
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- 2009
478. ONCOLOGY : TESTICULAR LEYDIGIOMA
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PAPPARELLA, Alfonso, R.O.M.A.N.O. M, PARMEGGIANI, Pio, ESPOSITO C., ESPOSITO G., ALBANESE C.T.,FUJIOKA M , MACKINLAY G. , ROLLINS M. , SHIER F, Papparella, Alfonso, R. O. M. A. N. O., M., and Parmeggiani, Pio
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- 2009
479. Case '63'
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LIMA, MARIO, RUGGERI, GIOVANNI, C. ESPOSITO G. ESPOSITO, M. Lima, and G. Ruggeri
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PEDIATRICS ,dyspnea - Abstract
The chapter is part of a collection of rare and interesting pediatric surgical cases. Case “63” is about a 1-year-old child suffered from dyspnea, cough mucous secretions and regurgitations for 2 weeks. Images from x-ray are shown. Fully case report is presented along with differential diagnosis.
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- 2009
480. Musculoskeletal disorder
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SADILE, FRANCESCO, F. CIGALA, C. Esposito, G. Esposito, Sadile, Francesco, and F., Cigala
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- 2009
481. GENITOURINARY DISORDERS : VARICOCELE
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PAPPARELLA, Alfonso, ROMANO M, PARMEGGIANI, Pio, ESPOSITO C., ESPOSITO G., ALBANESE C.T.,FUJIOKA M , MACKINLAY G. , ROLLINS M. , SHIER F, Papparella, Alfonso, Romano, M, and Parmeggiani, Pio
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- 2009
482. GENITOURINARY DISORDERS : SELF-AMPUTATED OVARIAN CYST
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PAPPARELLA, Alfonso, ROMANO M, PARMEGGIANI, Pio, ESPOSITO C., ESPOSITO G., ALBANESE C.T.,FUJIOKA M , MACKINLAY G. , ROLLINS M. , SHIER F, Papparella, Alfonso, Romano, M, and Parmeggiani, Pio
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- 2009
483. Endocrinologia e metabolismo
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PERRONE, Laura, F. PRISCO, IAFUSCO, Dario, A. LA MANNA, MIRAGLIA DEL GIUDICE, Emanuele, L PERRONE, C ESPOSITO, S GRANO, D IAFUSCO, Perrone, Laura, F., Prisco, Iafusco, Dario, A., LA MANNA, and MIRAGLIA DEL GIUDICE, Emanuele
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- 2008
484. An anti-inflammatory protein secreted from the rat seminal vesicle epithelium inhibits the synthesis of platelet-activating factor and the release of arachidonic acid and prostacyclin
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Carla Esposito, Raffaele Porta, Ciro Tetta, Gianfranco Peluso, Giovanni Camussi, Salvatore Metafora, Federico Bussolino, G., Camussi, C., Tetta, F., Bussolino, S., Metafora, G., Peluso, C., Esposito, and Porta, Raffaele
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Male ,Neutrophils ,Inflammation ,Prostacyclin ,Arachidonic Acids ,Biochemistry ,chemistry.chemical_compound ,Phospholipase A2 ,Mediator ,Acetyltransferases ,medicine ,Animals ,Humans ,Secretion ,Platelet Activating Factor ,biology ,Platelet-activating factor ,Tumor Necrosis Factor-alpha ,Macrophages ,Anti-Inflammatory Agents, Non-Steroidal ,Thrombin ,Proteins ,Seminal Vesicles ,respiratory system ,Epoprostenol ,Recombinant Proteins ,Rats ,chemistry ,Rats, Inbred Lew ,Acetyltransferase ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Arachidonic acid ,medicine.symptom ,medicine.drug - Abstract
Platelet-activating factor (PAF), a 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, is a mediator of inflam¬mation and endotoxic shock produced by a variety of stimulated cells. Since the main biosynthetic pathway of PAF involves acetylation of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) generated from 1-O-alkyl-2-acyl-¬sn-glycero-3-phosphocholine by phospholipase A2, we suggest a general physiological role played by steroid-¬induced anti-(phospholipase A2) proteins in the modulation of PAF synthesis. The results of the present study support this hypothesis since an androgen-induced anti-inflammatory protein, SV-IV, secreted from rat seminal vesicles, inhibits PAF synthesis in stimulated polymorphonuclear neutrophils, macrophages and endothelial cells. SV-IV impairs PAF synthesis by inhibiting the activation of phospholipase A2, that also results in the inhibition of arachidonic acid and prostacyclin release, and of acetyl-CoA: lyso-PAF acetyltransfe
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- 1990
485. Obestatin conformational features: a strategy to unveil obestatin's biological role?
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Pietro Campiglia, Cinzia Esposito, Anna Maria D'Ursi, Isabel Gomez-Monterrey, Ettore Novellino, Mario Scrima, M., Scrima, P., Campiglia, C., Esposito, GOMEZ MONTERREY, ISABEL MARIA, Novellino, Ettore, and A. M. D. ’. U. r. s., I.
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Models, Molecular ,Food intake ,Protein Conformation ,Molecular Sequence Data ,Biophysics ,Neuropeptide ,Biochemistry ,Protein Structure, Secondary ,In vivo ,Animals ,Amino Acid Sequence ,Receptor ,Molecular Biology ,Protein secondary structure ,Nuclear Magnetic Resonance, Biomolecular ,G protein-coupled receptor ,Chemistry ,Circular Dichroism ,Cell Biology ,Obestatin ,Ghrelin ,Peptide Fragments ,Protein Structure, Tertiary ,Rats ,Rat Stomach - Abstract
Obestatin and its derivative Ob(11-23) are recently discovered peptides produced in the rat stomach. They have proven to be involved in the regulation of energy balance, inhibiting feeding, causing reductions in food intake, body weight and jejunal contraction in rodents. The G-protein coupled receptor, GPR39, was originally proposed as being an obestatin target receptor, but this remains controversial. As such, the molecular mechanism for obestatin's effects in vivo is still uncertain. Here we report the CD and NMR conformational analysis of obestatin and Ob(11-23). Both peptides assume a regular secondary structure in the C-terminal region of the molecule. In this region, structural elements similar to other GPCR binding neuropeptides support the identity of obestatin as a new and functionally autonomous GPCR ligand. Conversely sequence and conformational specificity point to a new farmacoforic structure, on which innovative derivatives with a potential role in the treatment of obesity can be designed and synthetized.
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- 2007
486. Driving forces in the delivery of penetratin conjugated G protein fragment
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Anna Maria D'Ursi, Maria R. Mazzoni, Francesca Porchia, Paolo Rovero, Laura Giusti, G. Caliendo, Cinzia Esposito, Stefania Albrizio, Ettore Novellino, Gerardino D'Errico, Albrizio, Stefania, L., Giusti, D'Errico, Gerardino, C., Esposito, F., Porchia, G., Caliendo, Novellino, Ettore, M. R., Mazzoni, P., Rovero, and A. M., D’Ursi
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Models, Molecular ,Magnetic Resonance Spectroscopy ,Stereochemistry ,G protein ,Phosphorylcholine ,Static Electricity ,chimera peptide ,Peptide ,Cell-Penetrating Peptides ,Fluorescence ,Permeability ,chemistry.chemical_compound ,Surface-Active Agents ,Drug Discovery ,Fluorescence microscope ,GTP-Binding Protein alpha Subunits, Gs ,Sodium dodecyl sulfate ,Micelles ,chemistry.chemical_classification ,Vesicle ,Circular Dichroism ,Proteins ,Sodium Dodecyl Sulfate ,Membranes, Artificial ,Membrane transport ,Peptide Fragments ,Membrane ,chemistry ,Microscopy, Fluorescence ,Dipalmitoylphosphatidylcholine ,drug delivery ,liposome ,Molecular Medicine ,Carrier Proteins ,Peptides ,Hydrophobic and Hydrophilic Interactions - Abstract
A42 is a chimera peptide consisting of Galphas(374-394)C379A--the 21-mer C terminus of the Galphas protein, able of adenosine inhibitory activity--and penetratin--the 16 residue fragment, derived from the homeodomain of the Drosophila transcription factor Antennapedia. A42 is able to cross cell membranes and to inhibit A2A and A2B adenosine and beta-adrenergic receptor stimulated camps (D'Ursi et al. Mol. Pharmacol. 2006, 69, 727-36). Here we present an extensive biophysical study of A42 in different membrane mimetics, with the objective to evaluate the molecular mechanisms which promote the membrane permeation. Fluorescence, CD, and NMR data were acquired in the presence of negatively charged and zwitterionic sodium dodecyl sulfate and dodecylphosphocholine surfactants. To validate the spectroscopic results in a larger scale, fluorescence microscopy experiments were performed on negatively charged and zwitterionic dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine vesicles. Our results show that the internalization of A42 is mainly driven by electrostatic interactions, hydrophobic interactions playing only a secondary, sinergistic role. The distribution of the charges along the molecule has an important role, highlighting that internalization is a process which requires a specific matching of peptide and membrane properties.
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- 2007
487. Humoral immune response to tissue transglutaminase is related to epithelial cell proliferation in celiac disease
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Ivana Caputo, Riccardo Troncone, Roberto Marzari, Daniele Sblattero, Maria T. Ribecco, Maria Vittoria Barone, Maria Maglio, Salvatore Auricchio, Carla Esposito, Barone, MARIA VITTORIA, Caputo, I, Ribecco, Mt, Maglio, Mariantonia, Marzari, R, Sblattero, D, Troncone, Riccardo, Auricchio, Salvatore, Esposito, C., BARONE M., V, I., Caputo, M. T., Ribecco, M., Maglio, Marzari, Roberto, Sblattero, Daniele, R., Troncone, S., Auricchio, and AND C., Esposito
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Tissue transglutaminase ,Biopsy ,Cell ,Apoptosis ,celiac disease ,transglutaminase ,Pathogenesis ,Mice ,Immune system ,GTP-Binding Proteins ,medicine ,In Situ Nick-End Labeling ,Celiac disease ,ricombinant antibodies ,Animals ,Humans ,Protein Glutamine gamma Glutamyltransferase 2 ,Intestinal Mucosa ,autoantibodies ,Autoantibodies ,Cell Proliferation ,Transglutaminases ,Hepatology ,biology ,medicine.diagnostic_test ,Cell Cycle ,Gastroenterology ,Autoantibody ,Epithelial Cells ,autoantibodie ,Epithelium ,Actins ,Recombinant Proteins ,medicine.anatomical_structure ,Bromodeoxyuridine ,Immunology ,Antibody Formation ,biology.protein ,NIH 3T3 Cells ,Antibody ,Caco-2 Cells - Abstract
Background & Aims: Tissue transglutaminase (tTG) autoantibodies are markers of celiac disease, and the enzyme is required for several crucial biological processes. The aim of this study was to determine whether these autoantibodies are involved in the pathogenesis of the mucosal lesion typical of celiac disease. Methods: Using rhodamine-conjugated phalloidin staining, we evaluated whether tTG antibodies, both commercially available and cloned from patients with celiac disease, cause cytoskeletal changes in Caco-2, MCF7, and NIH 3T3 cells. We monitored cell levels of bromodeoxyuridine incorporation to determine whether tTG autoantibodies are able to induce NIH 3T3 fibroblasts and epithelial mucosal cells into S phase. Results: Treatment with tTG antibodies caused a dose-dependent increase of membrane ruffling in Caco-2, MCF7, and NIH 3T3 cells. It also dose-dependently induced G0-synchronized NIH 3T3 fibroblasts into S phase but did not affect the rate of apoptosis. Similarly, tTG antibodies induced S-phase entry of epithelial cells in cultured intestinal biopsy specimens from patients with celiac disease. They did not affect biopsy specimens from patients without celiac disease. Conclusions: Our results suggest that tTG autoantibodies per se, by interacting with the extracellular membrane–bound transglutaminase, may play an important role in epithelial cell proliferation in celiac disease.
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- 2007
488. Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of CYP2C9*2/*3 polymorphisms
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Rosanna Di Fiore, Pasquale Meccariello, Cristina Mazzaccara, Carmela Esposito, Lucia Sacchetti, Mario Toriello, M., Toriello, P., Meccariello, Mazzaccara, Cristina, R., DI FIORE, C., Esposito, and Sacchetti, Lucia
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Genetics ,Polymorphism, Genetic ,pharmacogenetic testing ,Medical screening ,Biochemistry (medical) ,Clinical Biochemistry ,Temperature ,General Medicine ,Computational biology ,Biology ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Clinical Practice ,Pharmacogenetics ,TaqMan ,Humans ,Taq Polymerase ,Aryl Hydrocarbon Hydroxylases ,Cytochrome P450 (CYP450) ,polymorphisms ,CYP2C9 ,Alleles ,Cytochrome P-450 CYP2C9 - Abstract
Background Pharmacogenetic testing for drug-metabolizing enzymes is not yet widely used in clinical practice. Methods In an attempt to facilitate the application of this procedure, we have compared two real-time PCR-based methods, the TaqMan and the LightCycler for the pharmacogenetic evaluation of CYP2C9*2/*3 polymorphisms. Results and conclusion Both procedures are suitable for pharmacogenetic studies. The TaqMan procedure was less expensive in terms of cost per sample, but the TaqMan apparatus is more expensive than the LightCycler apparatus.
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- 2006
489. Physicochemical characterization of a peptide deriving from the glycoprotein gp36 of the feline immunodeficiency virus and its lipoylated analogue in micellar systems
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Anna Maria D'Ursi, Simone Giannecchini, Paolo Rovero, Mauro Bendinelli, Gerardino D'Errico, Cinzia Esposito, Maria Rosaria Armenante, C., Esposito, D'Errico, Gerardino, M. R., Armenante, S., Giannecchini, M., Bendinelli, P., Rovero, and A. M., D'Ursi
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Feline immunodeficiency virus ,Protein Conformation ,Lipoproteins ,Phosphorylcholine ,Biophysics ,Peptide ,Immunodeficiency Virus, Feline ,Conformational analysi ,Antiviral Agents ,Biochemistry ,Lipopeptide ,Diffusion ,chemistry.chemical_compound ,Viral Envelope Proteins ,Nuclear Magnetic Resonance, Biomolecular ,Micelles ,Glycoproteins ,chemistry.chemical_classification ,biology ,Circular Dichroism ,Electron Spin Resonance Spectroscopy ,Sodium Dodecyl Sulfate ,Cell Biology ,biology.organism_classification ,In vitro ,FIV ,Conformational analysis ,Spectrometry, Fluorescence ,Membrane ,chemistry ,Viral replication ,Cell culture ,Micelle interface ,Glycoprotein - Abstract
P59 is the Trp-rich 20-mer peptide ( 767 L–G 786 ), partial sequence of the membrane-proximal external region (MPER) of the FIV gp36. It has potent antiviral activity, possibly due to a mechanism that inhibits the fusion of the virus with the cell membranes. In the hypothesis that a lipophilic tail could enhance the adhesion of P59 to the membrane so improving its antiviral activity, we synthesized its lipoylated analogue lipo-P59. Fluorescence, CD and NMR investigations in membrane mimicking environments (such as SDS and DPC micelles) were aimed to assess the potential of the lipo-P59 lipophilic tail to affect the biophysical and conformational behaviour of the peptide. In vitro inhibitory assays using lymphoid cell cultures to check the antiviral activity of peptides were also performed. The data show that the biophysical properties and the conformational preferences of the peptides are not dramatically affected by the hydrophobic tail, suggesting that the lipopeptide is capable of preserving all the biophysical peculiarities. Similarly, antiviral experimental data show that the membrane-anchored lipo-P59 peptide is also effective in inhibiting virus replication. Moreover, the lipophilic tail allows P59 to preserve its antiviral activity even in conditions in which the non lipoylated peptide is devoid of activity. In accordance with the unusual high Trp presence, the peptides confirm the preference to be positioned on the membrane interface. Furthermore, the data point out a peculiarity of interaction of the peptides with SDS as compared with DPC.
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- 2006
490. Clean Intermittent Catheterization
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Mario Lima, Paolo Messina, Laura Greco, Francesco Paolo Di Lorenzo, C. ESPOSITO J.M. GUYS D. GOUGH A. SAVANELLI, M. Lima, F.P. Di Lorenzo, P. Messina, and L. Greco
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Neurogenic bladder dysfunction ,business.industry ,Anesthesia ,Medicine ,Clean Intermittent Catheterization ,business - Abstract
In patients affected by Neurocgenic Bladder Dysfunctions (NBD), infrequent or incomplete voiding, thickness of the bladder wall and increased urethral resistance are risk factors for urinary tract infections as well as continence and urinary tract anomalies. A large majority of patients achieve an alleviation of the NBD symptoms with Clean Intermittent Catheterization (CIC). In order to achieve an effective treatment a correct diagnostic classification of the vescico-sphinteric dysfunction should be obtained. The chapter describes historical evolution of the management of patients with NBD, indications, training and materials required in CIC as well as complementary treatments and complications.
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- 2006
491. Exploring interaction of β-amyloid segment (25-35) with membrane models through paramagnetic probes
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M. Francesca Ottaviani, Mario Scrima, Gerardino D'Errico, Annamaria Tedeschi, Paolo Rovero, Cinzia Esposito, Anna Maria D'Ursi, C., Esposito, A., Tedeschi, M., Scrima, D'Errico, Gerardino, M. F., Ottaviani, P., Rovero, and A. M., D’Ursi
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Circular dichroism ,micelles ,Synthetic membrane ,Peptide ,Biochemistry ,Structural Biology ,Drug Discovery ,Drug Interactions ,Senile plaques ,Molecular Biology ,Peptide sequence ,Pharmacology ,chemistry.chemical_classification ,Amyloid beta-Peptides ,Circular Dichroism ,β-amyloid(25–35) ,Organic Chemistry ,Electron Spin Resonance Spectroscopy ,P3 peptide ,General Medicine ,Peptide Fragments ,Peptide Conformation ,Amino acid ,electron paramagnetic resonance ,chemistry ,Liposomes ,liposome ,Biophysics ,Molecular Medicine - Abstract
The accumulation of β-amyloid peptides into senile plaques is one of the hallmarks of Alzheimer's disease (AD). There is mounting evidence that the lipid matrix of neuronal cell membranes plays an important role in the β-sheet oligomerization process of β-amyloid. Aβ(25–35), the sequence of which is GSNKGAIIGLM, is a highly toxic segment of amyloid β (Aβ)-peptides, which forms fibrillary aggregates. In the present work, two spin-labelled Aβ(25–35) analogues containing the nitroxide group of the amino acid TOAC (2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) as a paramagnetic probe at the N- or the C-terminus of the peptide sequence, respectively, were synthesized in order to investigate the peptide-membrane interaction. The orientation and associated changes of the peptide conformation in the presence of different artificial membrane models (micelles, liposomes) were evaluated by electron paramagnetic resonance and circular dichroism techniques. The results of this study allowed us to propose a model in which the C-terminal portion of the peptide is highly associated to the membrane, while the N-terminal part extends into the aqueous phase with occasional contacts with the lipid head-group region. Interestingly, the interaction of the C-terminal portion of the peptide is particularly enhanced in the presence of sodium dodecyl sulfate (SDS) molecules. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.
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- 2006
492. Bladder neck reconstruction
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SAVANELLI, ANTONIO, ESPOSITO, CIRO, C. Esposito, J.M. Guys, D. Gough, A. Savanelli, Savanelli, Antonio, and Esposito, Ciro
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- 2006
493. Mitrofanoff principle in urinary reconstruction in children
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SAVANELLI, ANTONIO, ESPOSITO, CIRO, Valla J.S., C. Esposito, J.M. Guys, D. Gough, A. Savanelli, Savanelli, Antonio, Esposito, Ciro, and Valla, J. S.
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- 2006
494. Neurologic bladder
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ESPOSITO, CIRO, Mattioli G., Jasonni V., C. Esposito, J.M. Guys, D. Gough, A. Savanelli, Esposito, Ciro, Mattioli, G., and Jasonni, V.
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- 2006
495. Neurologic bladder dysfunction: a condition of increate risk of bladder malignancy
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ESPOSITO, CIRO, SAVANELLI, ANTONIO, De Fazio C., C. Esposito, J.M. Guys, D. Gough, A. Savanelli, Esposito, Ciro, De Fazio, C., and Savanelli, Antonio
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- 2006
496. Analysis of transglutaminase protein substrates by functional proteomics
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Margherita Ruoppolo, Alfonsina D'Amato, Gennaro Marino, Simona Francese, Stefania Orrù, Carla Esposito, Paolo Rovero, Ruoppolo, M, Orru, S, D'Amato, A, Francese, S, Rovero, P, Marino, Gennaro, Esposito, C., Ruoppolo, Margherita, S., Orru’, A., D’Amato, S., Francese, P., Rovero, G., Marino, and C., Esposito
- Subjects
Proteomics ,Time Factors ,Tissue transglutaminase ,Affinity label ,Biotin ,Peptide ,Biochemistry ,Chromatography, Affinity ,Substrate Specificity ,transglutaminase ,Amino Acid Sequence ,Amines ,Enzyme Inhibitors ,Molecular Biology ,Peptide sequence ,functional proteomic ,mass spectrometry ,chemistry.chemical_classification ,Transglutaminases ,biology ,Molecular Structure ,Affinity Labels ,Amino acid ,Enzyme ,chemistry ,Biotinylation ,For the Record ,biology.protein ,Peptides - Abstract
Transglutaminases are calcium-dependent enzymes that catalyze a post-translational modification of proteins through the formation of epsilon -(gamma-glutamyl)lysine bonds. Although specific roles for transglutaminases have been described, recent findings have provided evidence that dysregulation of transglutaminases may contribute to many pathological processes including celiac disease and neurodegenerative diseases. A crucial step in the elucidation of biological and pathological roles of transglutaminases requires the identification of protein substrates. A strategy based on a functional proteomic analysis was set up using two well-characterized biotinylated transglutaminase substrates as affinity probes: 5-(biotinamido)pentylamine and the synthetic biotinylated peptide TVQQEL, the amino- and acyl-donor probes, respectively. A pool of known tissue type transglutaminase protein substrates was selected in order to test the procedure. Results obtained in this paper indicate that the whole strategy can be successfully applied in order to identify transglutaminases protein substrates as well as the amino acid site sensitive toward enzyme activity.
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- 2003
497. Preparation and mechanical properties of edible pectin-soy flour films obtained in the absence or presence of transglutaminase
- Author
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Angela Sorrentino, Prospero Di Pierro, Raffaele Porta, Carla Esposito, Paolo Masi, Loredana Mariniello, Mariniello, Loredana, DI PIERRO, Prospero, C., Esposito, Sorrentino, Angela, Masi, Paolo, and Porta, Raffaele
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food.ingredient ,Pectin ,Tissue transglutaminase ,Surface Properties ,Flour ,Bioengineering ,Biocompatible Materials ,Raw material ,Applied Microbiology and Biotechnology ,food ,Tensile Strength ,Materials Testing ,Food science ,Transglutaminases ,biology ,Chemistry ,Food Packaging ,Soy Foods ,Membranes, Artificial ,General Medicine ,Apple pectin ,Elasticity ,Biodegradation, Environmental ,Polymerization ,Food ,Seeds ,biology.protein ,Pectins ,Soybeans ,Soy flour ,Biotechnology - Abstract
Whole soy flour and apple pectin were used as raw materials for producing hydrocolloid edible films. The best ratio between the two components (2:1 mg cm −2 , pectin–soy flour) was determined in order to obtain films which could be perfectly handled for their consistence. Films were also prepared in the presence of transglutaminase, an enzyme able to produce isopeptide bonds among the soy polypeptide chains. The latter films showed a smoother surface and higher homogeneity, as demonstrated by microstructural analyses, whereas studies on the mechanical properties indicated that transglutaminase increased their strength and reduced their flexibility. Our results suggest a possible use of the transglutaminase polymerized pectin–soy protein films as edible food or drug coatings.
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- 2003
498. Design of a home care for cardiologic chronic patients
- Author
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BRACALE, MARCELLO, BIFULCO P., CESARELLI M., SANSONE M., ESPOSITO C., Bracale, Marcello, Bifulco, Paolo, Cesarelli, Mario, Sansone, Mario, C., Esposito, Bifulco, P., Cesarelli, M., Sansone, M., and Esposito, C.
- Abstract
Sydney (Australia)
- Published
- 2003
499. Ubiquitination of tissue transglutaminase is modulated by interferon alpha in human lung cancer cells
- Author
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Alberto Abbruzzese, Michele Caraglia, Raffaele Porta, Anna Maria D'Alessandro, Gaia Giuberti, Anna Cozzolino, Carla Esposito, Monica Marra, C., Esposito, M., Marra, G., Giuberti, A. M., D'Alessandro, Porta, Raffaele, A., Cozzolino, M., Caraglia, A., Abbruzzese, Esposito, C, Marra, M, Giuberti, G, D'Alessandro, Am, Porta, R, Cozzolino, A, Caraglia, Michele, and Abbruzzese, A.
- Subjects
Proteasome Endopeptidase Complex ,Lung Neoplasms ,Tissue transglutaminase ,medicine.medical_treatment ,Lactacystin ,Retinoic acid ,Alpha interferon ,Apoptosis ,Biochemistry ,chemistry.chemical_compound ,Multienzyme Complexes ,medicine ,Tumor Cells, Cultured ,Humans ,Molecular Biology ,DNA Primers ,Transglutaminases ,biology ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Ubiquitin ,Interferon-alpha ,Cell Biology ,Flow Cytometry ,Molecular biology ,Cysteine Endopeptidases ,Cytokine ,chemistry ,Proteasome inhibitor ,biology.protein ,Growth inhibition ,medicine.drug ,Research Article - Abstract
The addition of 2500 i.u./ml interferon alpha (IFNalpha) for 48 h induced apoptosis, and caused an approx. 4-fold increase in the activity and expression of tissue transglutaminase (tTG), in human lung cancer H1355 cells. However, the increase in mRNA levels for tTG was just 1.6-fold. On the basis of these data, we investigated whether tTG levels may be regulated through regulation of its degradation via ubiquitination. It was found that 2500 i.u./ml IFNalpha induced a time-dependent decrease in tTG ubiquitination. On the other hand, addition of the proteasome inhibitor lactacystin led to accumulation of the ubiquitinated form of the enzyme and to a consequent increase in its expression. Treatment of the cells with the two agents combined antagonized the accumulation of the ubiquitinated isoforms of tTG induced by lactacystin and caused a potentiation of tTG expression. Moreover, the tTG inducer retinoic acid was also able to cause increased expression and ubiquitination of tTG in H1355 cells. The addition of monodansylcadaverine (a tTG inhibitor) to IFNalpha-treated H1355 cells completely antagonized growth inhibition and apoptosis induced by the cytokine. In conclusion, we demonstrate for the first time that tTG is ubiquitinated and degraded by a proteasome-dependent pathway. Moreover, IFNalpha can, at least in part, induce apoptosis through the modulation of this pathway.
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- 2003
500. Identification of tissue transglutaminase-reactive lysine residues in glyceraldehyde 3-phosphate dehydrogenase
- Author
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Simona Francese, Gennaro Marino, Margherita Ruoppolo, Stefania Orrù, Carla Esposito, Luigi Vitagliano, S., Orru’, Ruoppolo, Margherita, S., Francese, L., Vitagliano, G., Marino, C., Esposito, Orru, S, Francese, S, Vitagliano, L, Marino, Gennaro, and Esposito, C.
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Models, Molecular ,Time Factors ,Tissue transglutaminase ,Protein Conformation ,Swine ,Glutamine ,Lysine ,Blotting, Western ,Peptide ,Biochemistry ,Article ,Mass Spectrometry ,Protein Structure, Secondary ,Substrate Specificity ,Protein structure ,Western blot ,medicine ,Animals ,Molecular Biology ,Polyacrylamide gel electrophoresis ,137-146 (2002) ,Glyceraldehyde 3-phosphate dehydrogenase ,chemistry.chemical_classification ,Transglutaminases ,medicine.diagnostic_test ,biology ,Glyceraldehyde-3-Phosphate Dehydrogenases ,NAD ,Molecular biology ,Protein Structure, Tertiary ,Enzyme ,chemistry ,Microscopy, Fluorescence ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Rabbits ,Protein Science 11 ,Peptides ,Protein Binding - Abstract
Polyglutamine domains are excellent substrates for tissue transglutaminase resulting in the formation of cross-links with polypeptides containing lysyl residues. This finding suggests that tissue transglutaminase may play a role in the pathology of neurodegenerative diseases associated with polyglutamine expansion. The glycolytic enzyme GAPDH previously was shown to tightly bind several proteins involved in such diseases. The present study confirms that GAPDH is an in vitro lysyl donor substrate of tissue transglutaminase. A dansylated glutamine-containing peptide was used as probe for labeling the amino-donor sites. SDS gel electrophoresis of a time-course reaction mixture revealed the presence of both fluorescent GAPDH monomers and high molecular weight polymers. Western blot analysis performed using antitransglutaminase antibodies reveals that tissue transglutaminase takes part in the formation of heteropolymers. The reactive amino-donor sites were identified using mass spectrometry. Here, we report that of the 26 lysines present in GAPDH, K191, K268, and K331 were the only amino-donor residues modified by tissue transglutaminase.
- Published
- 2002
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