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Lead (Pb) is a serious toxic metal without any beneficial role in the biological system. Biochar (BC) has emerged as an excellent soil amendment to mitigate Pb toxicity. The impact of BC co-compost (BCC) in mitigating the toxic impacts of Pb has not been studied yet. Therefore, this study aimed to evaluate the potential of BC and BCC in improving the growth, physiological, and biochemical traits of Brassica napus and soil properties and reducing health risks (HR). The study was comprised of different Pb concentrations (control and 100 mg kg-1) and organic amendments (control, BC, compost, and BCC). The results indicated that Pb stress reduced the growth, photosynthetic pigments, seed yield, and oil contents by increasing hydrogen peroxide (H2O2) production and Pb uptake and accumulation in plant tissues and decreasing photosynthetic pigment and nutrient availability. The application of BCC alleviated the adverse impacts of Pb and improved seed production (40.24%) and oil yield (11.06%) by increasing chlorophyll a (43.18%) and chlorophyll b (25.58%) synthesis, relative water content (23.89%), total soluble protein (TSP: 23.14%), free amino acids (FAA: 26.47%), proline (30.98%), APX (40.90%), CAT (32.79%), POD (24.93%), and SOD (33.30%) activity. Biochar co-compost-mediated increase in seed and oil yield was also linked with a reduced accumulation of Pb in plant parts and soil Pb availability and improved the soil-available phosphorus, potassium, total nitrogen, soil organic carbon (SOC), and microbial biomass carbon (MBC). Furthermore, BCC also reduced the bioaccumulation concentration, daily metal intake, hazard index, and target hazard quotient. In conclusion, application of BCC can increase the growth, yield, and oil contents of Brassica napus by improving the physiological and biochemical traits and soil properties and reducing the Pb uptake. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) contamination has gained much attention due to its high degree of toxicity for living organisms. Therefore, different techniques are underway to eradicate HMs from the environment. Among the biological techniques, phytoremediation is a suitable method, but owing to the slow rate and chance of HMs penetration into the food chain, alternative techniques are needed to reduce their phytotoxicity, and biochar is one of them. Due to the diverse characteristics, biochar immobilizes HMs in the soil by improving soil pH, ion exchange, electrostatic interactions, complexation, precipitation, surface adsorption, and microbial activation. Thereby, amendment of biochar in the HMs-contaminated soils reduces HMs toxicity to plants and limits their penetration into the food chain. In contrast, some biochars have also been studied to induce metal availability in soils and subsequently its uptake by plants. This dual role of biochar depends on the feedstock of biochar, incineration temperature, and the rate of application. Moreover, biochar treatments enhance plant growth under HMs stress by improving nutrient availability, water retention capacity, scavenging of reactive oxygen species, and photosynthetic efficiency. Owing to the beneficial characteristics of biochar in HMs-contaminated sites, the number of publications has tremendously increased. Additionally, the plant species and the types of HMs that have been tested frequently under biochar treatments in these articles have been studied. Overall, the current study would help in understanding the mechanisms of how biochar influences phytoremediation of HMs and improves plant growth in HMs-polluted soils and the current scenario of the available literature. [ABSTRACT FROM AUTHOR]

Abstract\nNOVELTY STATEMENTHeavy metals and petroleum oil are the two most important contaminants in the environment. Currently, phytoremediation is regarded as an effective and affordable solution that allows the attenuation of toxic pollutants through the use of plants. Not many studies are carried out regarding the use of aromatic plants capable of remediating soil that is co-contaminated by heavy metal and petroleum hydrocarbons. A pot experiment was conducted to investigate the influence of cadmium-resistant PGPR Micrococcus luteus on the phytoremediation efficiency of Ocimum gratissimum in Cd and petroleum co-contaminated soil. The plants were harvested after 60 days of treatment and their growth and biomass were determined. The accumulation of Cd in plant shoots and roots was determined. The residual petroleum hydrocarbon concentration during the 60 days of the phytoremediation experiment was determined using GC-FID. O. gratissimum with M. luteus showed the highest Cd accumulation (14.05 mg kg−1) and the highest reduction of TPH (46.64%). M. luteus ameliorated contaminant toxicity and promoted biomass production of O. gratissimum. These results demonstrated that O. gratissimum in combination with M. luteus can be efficiently used to remediate Cd and petroleum-co-contaminated soils.The influence of cadmium-resistant PGPR, Micrococcus luteus, on the petroleum biodegradation efficiency of Ocimum gratissimum in Cd and petroleum-co-contaminated soil was found. M. luteus at the rhizosphere of O. gratissimum showed Cd-resistant capacity while reducing TPH at the highest removal efficiency within 60 days. [ABSTRACT FROM AUTHOR]

Coal mining disperses heavy metals into the environment, necessitating the identification of metal-tolerant plants for ecosystem restoration. This study evaluated the phytoremediation potential of plant species in abandoned coal wastes in northern Iran. Pollution indices indicated moderate contamination of Cu, Ni, V, Zn, Pb, Cr, and As in coal wastes. The plants varied in their ability to accumulate and translocate these metals, with most showing efficient root-to-shoot translocation. Artemisia scoparia (41.06 mg.kg−1) and Capparis spinosa (42.48 mg.kg−1) were effective for Cu phytoextraction. Most species, notably Cynodon dactylon (3.4 mg.kg−1), showed promise for phytoextraction of Cr. Capparis spinosa (7.67 mg.kg−1) exhibited potential for Pb phytoextraction. Most plants, particularly Hordeum vulgare and Melica persica, were effective phytoextractors of Ni. Sylibum marianum accumulated V beyond phytotoxic levels. Chenopodium album and Glaucium fimbriligerum were identified as phytoextractors of Zn while Cynodon dactylon and Hordeum vulgare, accumulating >100 mg.kg−1 Zn in roots, showed potential for phytostabilization. Sylibum marianum and Glaucium fimbriligerum, acted as excluders for As. Kochia prostrata and Artemisia aucheri were excluders for Cu, Cr, Ni, and Pb. This study provided the role of multiple indigenous plants, including perennials and annuals with diverse life forms, in metal extraction and stabilization for sustainable coal waste management. [ABSTRACT FROM AUTHOR]

Plant growth on mine wastes is restricted by the lack of water, nutrients, phytotoxic responses and the absence of a seedbank. In a mesocosm study, we addressed the establishment of vegetation on metalliferous mine wastes from two seed mixtures. Besides the composition of the vegetation and the increase in plant cover and biomass over time, we studied concentrations of heavy metals in the shoot and analyzed the quantity of throughflow, its pH and EC to follow pollutant discharge. We hypothesized that the types of mine wastes and sown grasslands will affect species composition and the formation of a protective plant cover. Our platform was well-suited to study build-up and succession of a vegetation layer and its potential to stabilize mine wastes. However, the establishing community was less diverse than expected. The dilution of wastes increased species number and biomass, and we found a reduction of material discharge with increasing vegetation cover. Over time, drainage was reduced, while pH of the throughflow did not change. However, it was higher under the addition of greywater. Interestingly, the use of greywater led to a higher biomass in one mixture and slight changes in the chemistry of the throughflow and the plant matter. STATEMENT OF NOVELTY: Here, we present an integrative method to test the greening potentials of mine wastes. In the mesocosm approach different mine wastes, additives and seed mixtures can be screened and the potential of the establishing vegetation to reduce drainage and runoff may be addressed at the same time. Furthermore, analyses of pollutants in plants, soil substrates and drainage waters serve to study the phytoextraction and phytostabilization potentials of the established vegetation and their ecological services. [ABSTRACT FROM AUTHOR]

Green areas, in particular lawns, play important roles in cities. Unfortunately, they are often arranged in sites contaminated with heavy metals. This study analyzed soils and lawn swards in three districts of the city of Wrocław. Three different categories of lawns were examined: residential areas, street lawns and parks. Particular focus was placed on soil contamination with the metals Zn, Cu, Cd and Pb and their accumulation in the aboveground biomass of the perennial ryegrass Lolium perenne, the main grass species, and in the biomass of other components of the lawn sward. The research revealed local occurrence of elevated metal concentrations in soils, although in most of the studied sites, they did not exceed the safe values set byPolish law. The accumulation of metals in the aboveground parts of ryegrass and other plants forming the lawn sward depended primarily on the type of metal. The study confirmed the high phytoavailability of cadmium and zinc and the very low phytoavailability of lead. Perennial ryegrass accumulated considerably lower amounts of lead and copper compared with other components of the lawn sward, which indicates the potential suitability of this species for the phytostabilization and sustainable development of areas contaminated with these two metals. [ABSTRACT FROM AUTHOR]

Although the phytoremediation strategy has been studied worldwide, little research data are available regarding the influence of mycorrhizae on the phytoremediation capacity of various plants grown in Cd-contaminated soils in Mediterranean environments. Therefore, a pot experiment was carried out to study the possible effectiveness of hemp plant (Cannabis sativa L.) in the remediation of moderately and heavily Cd-contaminated soils and additionally to quantify the effect of Cd on Arbuscular Mycorrhizal Fungi (AMFs). For this purpose, an alkaline clay soil collected from the Farm of Institute of Plant Breeding and Genetic Resources (North Greece) was contaminated with two levels of Cd (3 and 30 mg Cd kg−1, corresponding to Levels A and B, respectively—first factor) at two incubation times (10 and 30 days—second factor) and six treatments (Control_30d, Control_10d, CdA_30d, CdB_30d, CdA_10d, CdB_10d) were created. Soil Cd concentrations, both pseudo-total and available to plants, were determined after extraction with Aqua Regia mixture and DTPA solution, respectively, before and after the cultivation of hemp plants and after the harvesting. Cd concentrations in the aboveground and underground plant parts were also estimated after digestion with Aqua Regia, while root colonization by AMFs was determined with a microscope. The highest plant's Cd concentration, more than 50%, was observed in its underground part, at all Cd-contaminated treatments, indicating a strong capacity for cadmium to gather up in the roots. Among different Cd levels and incubation days, significant differences were recorded in the rates of root colonization by AMFs. Among different Cd levels and incubation days, 3 mg Cd Kg−1 soil promoted AMF root colonization, particularly at 10-day incubation, while 30 mg Cd Kg−1 soil diminished it. Colonization was lower with longer incubation times at both levels of Cd. Hemp appears to be a viable option for phytostabilization in Cd-contaminated soils, enabling further utilization of AMFs to assist the phytoremediation process. [ABSTRACT FROM AUTHOR]

The increasing demand for mineral resources has generated mine tailings with heavy metals (HM) that negatively impact human and ecosystem health. Therefore, it is necessary to implement strategies that promote the immobilization or elimination of HM, like phytoremediation. However, the toxic effect of metals may affect plant establishment, growth, and fitness, reducing phytoremediation efficiency. Therefore, adding organic amendments to mine tailings, such as biochar, can favor the establishment of plants, reducing the bioavailability of HM and its subsequent incorporation into the food chain. Here, we evaluated HM bioaccumulation, biomass, morphological characters, chlorophyll content, and genotoxic damage in the herbaceous Crotalaria pumila to assess its potential for phytostabilization of HM in mine tailings. The study was carried out for 100 days on plants developed under greenhouse conditions under two treatments (tailing substrate and 75% tailing/25% coconut fiber biochar substrate); every 25 days, 12 plants were selected per treatment. C. pumila registered the following bioaccumulation patterns: Pb > Zn > Cu > Cd in root and in leaf tissues. Furthermore, the results showed that individuals that grew on mine tailing substrate bioaccumulated many times more metals (Zn: 2.1, Cu: 1.8, Cd: 5.0, Pb: 3.0) and showed higher genetic damage levels (1.5 times higher) compared to individuals grown on mine tailing substrate with biochar. In contrast, individuals grown on mine tailing substrate with biochar documented higher chlorophyll a and b content (1.1 times more, for both), as well as higher biomass (1.5 times more). Therefore, adding coconut fiber biochar to mine tailing has a positive effect on the establishment and development of C. pumila individuals with the potential to phytoextract and phytostabilize HM from polluted soils. Our results suggest that the binomial hyperaccumulator plant in combination with this particular biochar is an excellent system to phytostabilize soils contaminated with HM. [ABSTRACT FROM AUTHOR]

Several mining-related pollutions, industrial waste, and soil deterioration define South Africa's environmental landscape. These have led to the consumption of unhealthy food, contaminated agricultural products, and polluted water. The polluted environment has been linked to numerous diseases among the populace, thus making environmental remediation an important issue in South Africa. Phytoremediation has been identified as a biological method for the restoration of polluted environments naturally and holistically. Therefore, it is vital to evaluate the level of phytoremediation-related research in South Africa in pursuit of a way out of environmental pollution. Thus, the purpose of this study was to map phytoremediation-related research in South Africa from inception to 2022. Statistical records from the Web of Science Core Collection were analyzed with the bibliometric package in RStudio, while mapping was performed via VOSviewer. Our study showed a low annual growth rate of publication (4.49%). The analysis uncovered that the 39 documents analyzed were written by 112 authors, and the first document was featured in the Journal of Geochemical Exploration in 1997. Kirkham, MB and Liphadzi, MS are the most relevant authors. USA has the strongest collaboration with South Africa, while the International Journal of Phytoremediation, the South African Journal of Botany, and Water SA are the most relevant journals. The result of this study can guide upcoming researchers and policymakers, together with essential facts for enhancing the restoration of the polluted environment in the country. [ABSTRACT FROM AUTHOR]

Mercury (Hg) pollution in Ghana through mining has become a serious environmental challenge. This study investigates the potential of Cirsium arvense to photostabilize Hg using electrokinetic current with or without an iodide solution in gold mine tailings heavily contaminated through mining activities in southern Ghana. An initial Hg concentration of 9.60 mg/kg using cold vapor atomic absorption spectrometry (CVAAS) was determined. The biological absorption coefficient, bioconcentration factor, and translocation factor of Hg have been presented. Cirsium arvense therefore had a higher bioconcentration factor (BCF) of 2.6–5.15 mg/kg, and a transfer factor (TF) of 0.24–0.36 indicating a higher efficiency for phytostabilization. Both the rate and time of extractions of Hg from the tailings by Cirsium arvense are efficiently improved in the combined electric current and iodide treatment. Plant and electric current combined treatment and plant and iodide combined treatment had only 60 and 50% phytostabilization rates, respectively. The combined plant, iodide, and electric current treatment has proven to be superior with about >90% Hg removal rate. Therefore, the combined plant, iodide, and electric current treatment resulted in a higher Hg removal efficiency by Cirsium arvense in a shorter period due to higher solubilization rate and electromigration effects on Hg species. [ABSTRACT FROM AUTHOR]

Abstract Arid regions can benefit from using native desert plants, which require minimal freshwater and can aid in remediating soil phytotoxic metals (PTMs) from traffic emissions. In this study, we assessed the ability of three native desert plants—Pennisetum divisum, Tetraena qatarensis, and Brassica tournefortii—to accumulate phytotoxic metals (PTMs) in their different plant organs, including leaves, stems, and roots/rhizomes. The PTMs were analyzed in soil and plant samples collected from Dubai, United Arab Emirates (UAE). The results indicated significantly higher levels of PTMs on the soil surface than the subsurface layer. Brassica exhibited the highest concentrations of Fe and Zn, measuring 566.7 and 262.8 mg kg−1, respectively, while Tetraena accumulated the highest concentration of Sr (1676.9 mg kg−1) in their stems. In contrast, Pennisetum recorded the lowest concentration of Sr (21.0 mg kg−1), while Tetraena exhibited the lowest concentrations of Fe and Zn (22.5 and 30.1 mg kg−1) in their leaves. The roots of Pennisetum, Brassica, and Tetraena demonstrated the potential to accumulate Zn from the soil, with concentration factors (CF) of 1.75, 1.09, and 1.09, respectively. Moreover, Brassica exhibited the highest CF for Sr, measuring 2.34. Pennisetum, however, could not translocate PTMs from its rhizomes to other plant organs, as indicated by a translocation factor (TF) of 1. In contrast, Brassica effectively translocated the studied PTMs from its roots to the stem and leaves (except for Sr in the leaves). Furthermore, Pennisetum exclusively absorbed Zn from the soil into its leaves and stems, with an enrichment factor (EF) greater than 1. Brassica showed the ability to uptake the studied PTMs in its stem and leaves (except for Fe), while Tetraena primarily absorbed Sr and Zn into its stems. Based on the CF and TF results, Pennisetum appears to be a suitable species for phytostabilization of both Fe and Zn, while Brassica is well-suited for Sr and Zn polluted soils. Tetraena shows potential for Zn phytoremediation. These findings suggest that these plants are suitable for PTMs phytoextraction. Furthermore, based on the EF results, these plants can efficiently sequester PTMs.

Many contaminated tailings throughout the world cause environmental and human-health related problems due to air and water drift. Tailing phytostabilization is a promising solution, but only certain plant species may tolerate and grow in these contaminated areas. We analyzed the chemical properties of a vegetated and unvegetated area in a tailing site in Central Chile. In addition, in the vegetated area we analyzed the metals content of roots, stems, and foliage in 41-years old plantations of Pinus radiata, Acacia dealbata, and Eucalyptus globulus (the only three species that survived from a total of 34 species planted), and determined height (H), and diameter at breast height (DBH). The results indicated that, except for pH, Se, Pb, and organic matter, all components (nutrients and metals) were two- to three- fold lower in the vegetated tailing compared to that of the unvegetated tailing. The analysis of plant tissues indicated that Cu was higher in the roots of P. radiata (2,073 mg kg−1) and lower in the stems of the same species (4.1 mg kg−1). However, the ability to take up and transport Cu to the shoots was higher in A. dealbata and lower in P. radiata (bioaccumulation factor of 0.19 and 0.06, respectively). Here we present results for the first long-term phytostabilization project of copper mine tailings in Chile. From the 34 native and exotic species established in 1980 in a mine tailing disposal site with 1,000 mg Cu kg−1, only the exotic Pinus radiata, Acacia dealbata and Eucalyptus globulus were able to survive and adapt to the tailing conditions the last 41 years. This corroborates their potential for the future phytostabilization of copper mine wastes. [ABSTRACT FROM AUTHOR]

Phytoremediation is a useful, low-cost, and environmentally friendly alternative for the rehabilitation of heavy-metal-contaminated (HM) soils. This technology takes advantage of the ability of certain plant species to accumulate HMs in their tissues. Crotalaria pumila is a herbaceous plant with a wide geographical distribution that grows naturally in environments polluted with HMs. In this work, the bioaccumulation capacity of roots and leaves in relation to five HMs (Cr, Cu, Fe, Pb, and Zn) was evaluated, as well as the morphological changes presented in C. pumila growing in control substrate (without HMs) and mine-tailing substrate (with HMs) under greenhouse conditions for 150 days. Four metals with the following concentration pattern were detected in both tissues and substrates: Fe > Pb > Cu > Zn. Fe, Pb, and Zn concentrations were significantly higher in the roots and leaves of individuals growing on mine-tailing substrate compared to the control substrate. In contrast, Cu concentration increased over time in the exposed individuals. The bioconcentration factor showed a similar pattern in root and leaf: Cu > Fe > Pb > Zn. Around 87.5% of the morphological characters evaluated in this species decreased significantly in individuals exposed to HMs. The bioconcentration factor shows that C. pumila is efficient at absorbing Cu, Fe, and Pb from the mine-tailing substrate, in the root and leaf tissue, and the translocation factor shows its efficiency in translocating Cu from the roots to the leaves. Therefore, C. pumila may be considered as a HM accumulator plant with potential for phytoremediation of polluted soils with Cu, Pb, and Fe, along with the ability to establish itself naturally in contaminated environments, without affecting its germination rates. Also, it exhibits wide geographical distribution, it has a short life cycle, exhibits rapid growth, and can retain the mine-tailing substrate, extracting HMs in a short time. [ABSTRACT FROM AUTHOR]

Many anthropogenic activities have lately resulted in soil adulteration by heavy metals (HMs). The assessment of native plant species that grow in the polluted environments is of great importance for using these plants in phytoremediation techniques. This study was conducted in three industrial regions in Jeddah city, Wadi Marik, Bahra, and Khumrah, to assess the HM contamination level in them. This study also evaluated the phytoremediation ability of nine plant species collected from the studied regions. Soil physicochemical properties of the studied sites were investigated. Nine HMs, aluminum (Al), nickel (Ni), zinc (Zn), cobalt (Co), iron (Fe), lead (Pb), manganese (Mn), chromium (Cr), and barium (Ba), have been evaluated in the collected soil, plant shoots, and root samples. Total thiol concentration in the plant shoots and roots was determined. The phytoremediation indexes, such as bioaccumulation factor (BCF) and translocation factor (TF), were estimated. The results show that the soil of all the explored sites was sandy and slightly alkaline. It was found that Ni, Pb, and Cr were above the international permissible limit in all soil samples. The Wadi Marik region recorded the highest HM concentration compared to the other sites. In the Bahra region, Fe, Zn, Co, and Mn in all collected soil samples were below internationally permissible levels. In Khumrah, the highest concentration of Zn was found in the soil sample collected around F. indica plants, while Fe, Co, and Mn in all collected soil samples were below the international permissible limit. Depending on the BCF calculations, most of the investigated species showed phytostabilization ability for most of the studied HMs. Of them, E. indica, T. nubica, and P. divisum recorded the highest BCF values that ranged from 16.1 to 3.4. The BCF values of the studied HMs reduced in the order of Cr > Zn > Mn > Co > Ba > Fe > Al > Pb. Phytoextration of Co and Cr could be achieved by P. oleracea and F. indica, which showed TF values that reached 6.7 and 6.1, respectively. These plants showed high potential for phytoremediation and can be suggested as protective belts close to the contaminated regions of Jeddah. [ABSTRACT FROM AUTHOR]

Lead (Pb) is a common toxic heavy metal element that can be absorbed by plant roots and enter the food chain, damaging human health. Alnus cremastogyne has a wide native range, is fast growing, has a wide range of timber uses, and has rhizomatous roots that can improve planted soils. In this study, we evaluated whether Alnus cremastogyne has the potential to remediate Pb-contaminated soils through a 6-month pot experiment in soils with different Pb concentrations (0, 50, 100, 200, and 400 mg/kg). Our results indicate that Alnus cremastogyne plant height, basal diameter, and organ biomass decreased, H2O2 and MDA content increased, and the activities of antioxidant enzymes and osmotic regulators increased and then decreased with increasing lead concentrations. The Pb bioconcentration factor of Alnus cremastogyne was less than 1 at all Pb concentrations, and Pb accumulated mainly in the root system. This indicates that Alnus cremastogyne is not a Pb-enriched plant and does not have outstanding Pb transport capacity. The growth of Alnus cremastogyne was not significantly affected at low Pb concentrations, and its plant height, basal diameter, and biomass were significantly suppressed under high Pb stress. Therefore, Alnus cremastogyne is not a suitable species for the remediation of lead-contaminated soils but can be used as a silvicultural species in environments with low lead levels. [ABSTRACT FROM AUTHOR]

In the current study, the Cu phytoremediation ability of two ornamental plants, Chrysanthemum indicum L. and Tagetes erecta L., was tracked concerning the growth and physiological responses. Plants were subjected to varying concentrations of Cu (0, 100, 200, and 400 mg/kg) under the pot experiment for 8 weeks. The results showed that the measured growth and physiological characteristics declined in T. erecta shoots and roots at all tested treatments compared with the control. However, in C. indicum at 100 mg/kg, shoot biomass, shoot total soluble protein, and leaves number remained equal to that of the control and then reduced by rising Cu concentrations, compared with the control. Also, results indicated that in C. indicum, after 56 days of exposure to Cu, the chlorophyll pigments content markedly increased and reached a maximum level at 100 mg/kg dose and gradually declined with enhancing Cu concentrations, compared with the control. Other measured growth and physiological parameters decreased in both tissues of C. indicum in response to Cu usage in the growth medium. The carotenoid content of T. erecta decreased in all studied Cu levels in comparison to the control, but in C. indicum remained unaffected up to 200 mg/kg Cu in comparison to the control and then enhanced with increasing Cu level. The augmentation of antioxidant enzyme activity in two species, especially in roots, reflected the incident of Cu stress as demonstrated by elevated MDA and ion leakage levels. Data concerning copper accumulation in tissues, TF, and BAF showed T. erecta is a weak Cu accumulator and seems not to be an appropriate candidate for Cu phytoremediation. However, the Cu content in shoots and roots of C. indicum increased significantly with an increment in applied Cu level. Also, C. indicum accumulated higher Cu concentrations in the roots than in shoots and exhibited TF < 1, 0.1 < BAF root < 1, and can be considered as a Cu excluder by the phytostabilization mechanism. [ABSTRACT FROM AUTHOR]

Arid regions can benefit from using native desert plants, which require minimal freshwater and can aid in remediating soil phytotoxic metals (PTMs) from traffic emissions. In this study, we assessed the ability of three native desert plants—Pennisetum divisum, Tetraena qatarensis, and Brassica tournefortii—to accumulate phytotoxic metals (PTMs) in their different plant organs, including leaves, stems, and roots/rhizomes. The PTMs were analyzed in soil and plant samples collected from Dubai, United Arab Emirates (UAE). The results indicated significantly higher levels of PTMs on the soil surface than the subsurface layer. Brassica exhibited the highest concentrations of Fe and Zn, measuring 566.7 and 262.8 mg kg−1, respectively, while Tetraena accumulated the highest concentration of Sr (1676.9 mg kg−1) in their stems. In contrast, Pennisetum recorded the lowest concentration of Sr (21.0 mg kg−1), while Tetraena exhibited the lowest concentrations of Fe and Zn (22.5 and 30.1 mg kg−1) in their leaves. The roots of Pennisetum, Brassica, and Tetraena demonstrated the potential to accumulate Zn from the soil, with concentration factors (CF) of 1.75, 1.09, and 1.09, respectively. Moreover, Brassica exhibited the highest CF for Sr, measuring 2.34. Pennisetum, however, could not translocate PTMs from its rhizomes to other plant organs, as indicated by a translocation factor (TF) of 1. In contrast, Brassica effectively translocated the studied PTMs from its roots to the stem and leaves (except for Sr in the leaves). Furthermore, Pennisetum exclusively absorbed Zn from the soil into its leaves and stems, with an enrichment factor (EF) greater than 1. Brassica showed the ability to uptake the studied PTMs in its stem and leaves (except for Fe), while Tetraena primarily absorbed Sr and Zn into its stems. Based on the CF and TF results, Pennisetum appears to be a suitable species for phytostabilization of both Fe and Zn, while Brassica is well-suited for Sr and Zn polluted soils. Tetraena shows potential for Zn phytoremediation. These findings suggest that these plants are suitable for PTMs phytoextraction. Furthermore, based on the EF results, these plants can efficiently sequester PTMs. [ABSTRACT FROM AUTHOR]

The present study of phytomitigation potential and adaptive physiological and biochemical responses of helophyte Typha latifolia L. growing in water bodies at different distances from the century-old copper smelter (JSC "Karabashmed" Chelyabinsk Region, Russia) was conducted for the first time. This enterprise is one of the most dominant sources of multi-metal contamination for water and land ecosystems. The aim of the research was to assess the heavy metal (Cu, Ni, Zn, Pb, Cd, Mn, and Fe) accumulation, the photosynthetic pigment complex, and some redox reactions in T. latifolia from six differently technogenic impacted sites. In addition, the quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM) in rhizosphere sediments, as well as some plant growth-promoting (PGP) attributes of 50 isolates from each site, were determined. The water and sediment metal concentrations in highly contaminated sites exceeded the permissible/critical limits and were found much higher than that previously reported by other researchers while studying this helophyte. Both the degree of contamination and geoaccumulation indexes further elucidated extremely high contamination due to prolonged activity of copper smelter. T. latifolia accumulated significantly higher concentrations of the most of studied metals in its roost and rhizome with meager transfer to leaves (the translocation factors were less than one). Spearman's rank correlation coefficient showed a strong positive correlation between the metal concentration in sediments and its content in T. latifolia leaves (rs = 0.786 at p < 0.001 on average) and roots/rhizome (rs = 0.847 at p < 0.001 on average). In highly contaminated sites, the folia content of chlorophyll a and carotenoids decreased (by 30 and 38%, respectively), while lipid peroxidation enhanced (by 42%) on average compared to S1–S3 sites. These responses were accompanied by increasing non-enzymatic antioxidant content (soluble phenolic compounds, free proline, and soluble thiols) that allow plants to resist under significant anthropogenic loads. QMAFAnM in the five studied rhizosphere substrates varied insignificantly (2.5 × 106 − 3.8 × 107 cfu g−1 DW) and was decreased only in the most contaminated site (4.5 × 105). The proportion of rhizobacteria capable of fixing atmospheric nitrogen decreased by 1.7 times, solubilizing phosphates by 1.5 times, and synthesizing indol-3-acetic acid by 1.4 times in highly contaminated sites, while the amount of siderophore, 1-aminocyclopropane-1-carboxylate deaminase, and HCN producing bacteria did not considerably change. The results indicate high resistance of T. latifolia to prolonged technogenic impact, probably due to compensatory adaptive changes in the nonenzymatic antioxidant level and presence of beneficial microorganisms. Thus, T. latifolia was found to be a promising metal-tolerant helophyte that could help in mitigation of metal toxicity due to their phytostabilization even in heavily contaminated environment. [ABSTRACT FROM AUTHOR]

Lead (Pb) is a serious toxic metal without any beneficial role in the biological system. Biochar (BC) has emerged as an excellent soil amendment to mitigate Pb toxicity. The impact of BC co-compost (BCC) in mitigating the toxic impacts of Pb has not been studied yet. Therefore, this study aimed to evaluate the potential of BC and BCC in improving the growth, physiological, and biochemical traits of Brassica napus and soil properties and reducing health risks (HR). The study was comprised of different Pb concentrations (control and 100 mg kg-1) and organic amendments (control, BC, compost, and BCC). The results indicated that Pb stress reduced the growth, photosynthetic pigments, seed yield, and oil contents by increasing hydrogen peroxide (H2O2) production and Pb uptake and accumulation in plant tissues and decreasing photosynthetic pigment and nutrient availability. The application of BCC alleviated the adverse impacts of Pb and improved seed production (40.24%) and oil yield (11.06%) by increasing chlorophyll a (43.18%) and chlorophyll b (25.58%) synthesis, relative water content (23.89%), total soluble protein (TSP: 23.14%), free amino acids (FAA: 26.47%), proline (30.98%), APX (40.90%), CAT (32.79%), POD (24.93%), and SOD (33.30%) activity. Biochar co-compost-mediated increase in seed and oil yield was also linked with a reduced accumulation of Pb in plant parts and soil Pb availability and improved the soil-available phosphorus, potassium, total nitrogen, soil organic carbon (SOC), and microbial biomass carbon (MBC). Furthermore, BCC also reduced the bioaccumulation concentration, daily metal intake, hazard index, and target hazard quotient. In conclusion, application of BCC can increase the growth, yield, and oil contents of Brassica napus by improving the physiological and biochemical traits and soil properties and reducing the Pb uptake.

Abstract The concentration of copper in the soil increased with the intensification of agricultural activities, mainly in grape production areas and orchards as a result of the application of pesticides. Arbuscular mycorrhizal fungi make up the microbial biomass of the soil and appear as an alternative to be researched for the development of plants in an environment contaminated with copper. The purpose of this pot study was to analyze the influence of arbuscular mycorrhizal fungi on the development and content of copper in corn and sorghum plants. Soil treatments were: without inoculum (control) and two arbuscular mycorrhizal (Acaulospora scrobiculata and Rhizoglomus clarum) and five doses of copper (0, 100, 200, 300, and 400 mg Cu kg-1 soil); with seven repetitions. Plant height, stem diameter, number of tillers, root volume, shoot and root dry weight yields, shoot, root and grain Cu concentrations, pseudo-total soil Cu, percentage of mycorrhizal colonization and relative mycorrhizal efficiency index in reducing Cu concentration in root and shoot of corn and sorgum were evaluated. Morphological parameters of sorghum and corn were reduced with at high Cu doses in the soil, and the inoculation with Acaulospora scrobiculata and Rhizoglomus clarum resulted in greater development and lower Cu concentration in the dry mass of the shoot and root parts sorghum and corn plants.

Abstract Heavy metals (HMs) contamination, owing to their potential links to various chronic diseases, poses a global threat to agriculture, environment, and human health. Nickel (Ni) is an essential element however, at higher concentration, it is highly phytotoxic, and affects major plant functions. Beneficial roles of plant growth regulators (PGRs) and organic amendments in mitigating the adverse impacts of HM on plant growth has gained the attention of scientific community worldwide. Here, we performed a greenhouse study to investigate the effect of indole-3-acetic acid (IAA @ 10− 5 M) and compost (1% w/w) individually and in combination in sustaining cauliflower growth and yield under Ni stress. In our results, combined application proved significantly better than individual applications in alleviating the adverse effects of Ni on cauliflower as it increased various plant attributes such as plant height (49%), root length (76%), curd height and diameter (68 and 134%), leaf area (75%), transpiration rate (36%), stomatal conductance (104%), water use efficiency (143%), flavonoid and phenolic contents (212 and 133%), soluble sugars and protein contents (202 and 199%), SPAD value (78%), chlorophyll ‘a and b’ (219 and 208%), carotenoid (335%), and NPK uptake (191, 79 and 92%) as compared to the control. Co-application of IAA and compost reduced Ni-induced electrolyte leakage (64%) and improved the antioxidant activities, including APX (55%), CAT (30%), SOD (43%), POD (55%), while reducing MDA and H2O2 contents (77 and 52%) compared to the control. The combined application also reduced Ni uptake in roots, shoots, and curd by 51, 78 and 72% respectively along with an increased relative production index (78%) as compared to the control. Hence, synergistic application of IAA and compost can mitigate Ni induced adverse impacts on cauliflower growth by immobilizing it in the soil.

Abstract Arsenic (As) is a heavy metal that is toxic to both plants and animals. Silicon nanoparticles (SiNPs) can alleviate the detrimental effects of heavy metals on plants, but the underlying mechanisms remain unclear. The study aims to synthesize SiNPs and reveal how they promote plant health in Arsenic-polluted soil. 0 and 100% v/v SiNPs were applied to soil, and Arsenic 0 and 3.2 g/ml were applied twice. Maize growth was monitored until maturity. Small, irregular, spherical, smooth, and non-agglomerated SiNPs with a peak absorbance of 400 nm were synthesized from Pycreus polystachyos. The SiNPs (100%) assisted in the development of a deep, prolific root structure that aided hydraulic conductance and gave mechanical support to the maize plant under As stress. Thus, there was a 40–50% increase in growth, tripled yield weights, and accelerated flowering, fruiting, and senescence. SiNPs caused immobilization (As(III)=SiNPs) of As in the soil and induced root exudates Phytochelatins (PCs) (desGly-PC2 and Oxidized Glutathione) which may lead to formation of SiNPs=As(III)–PCs complexes and sequestration of As in the plant biomass. Moreover, SiNPs may alleviate Arsenic stress by serving as co-enzymes that activate the antioxidant-defensive mechanisms of the shoot and root. Thus, above 70%, most reactive ROS (OH) were scavenged, which was evident in the reduced MDA content that strengthened the plasma membrane to support selective ion absorption of SiNPs in place of Arsenic. We conclude that SiNPs can alleviate As stress through sequestration with PCs, improve root hydraulic conductance, antioxidant activity, and membrane stability in maize plants, and could be a potential tool to promote heavy metal stress resilience in the field.

Soil toxicity is a major environmental issue that leads to numerous harmful effects on plants and human beings. Every year a huge amount of Pb is dumped into the environment either from natural sources or anthropogenically. Being a heavy metal it is highly toxic and non-biodegradable but remains in the environment for a long time. It is considered a neurotoxic and exerts harmful effects on living beings. In the present review article, investigators have emphasized the side effects of Pb on the plants. Further, the authors have focused on the various sources of Pb in the environment. Investigators have emphasized the various responses including molecular, biochemical, and morphological of plants to the toxic levels of Pb. Further emphasis was given to the effect of elevated levels of Pb on the microbial population in the rhizospheres. Further, emphasized the various remediation strategies for the Pb removal from the soil and water sources.

The addition of soil amendments and plants in heavy metal-contaminated soil can result in a significant impact on physicochemical properties, microbial communities and heavy metal distribution, but the specific mechanisms remain to be explored. In this study, Koelreuteria paniculata was used as a test plant, spent mushroom compost (SMC) and attapulgite (ATP) were used as amendments, and manganese slag was used as a substrate. CK (100% slag), M0 (90% slag + 5% SMC + 5% ATP) and M1 (90% slag + 5% SMC + 5% ATP, planting K. paniculata) groups were assessed in a pilot-scale experiment to explore their different impacts on phytoremediation. The results indicated that adding the amendments significantly improved the pH of the manganese slag, enhancing and maintaining its fertility and water retention. Adding the amendments and planting K. paniculata (M1) significantly reduced the bioavailability and migration of heavy metals (HMs). The loss of Mn, Pb and Zn via runoff decreased by 15.7%, 8.4% and 10.2%, respectively, compared to CK. K. paniculata recruited and enriched beneficial fungi, inhibited pathogenic fungi, and a more stable fungal community was built. This significantly improved the soil quality, promoted plant growth and mitigated heavy metal toxicity. In conclusion, this study demonstrated that the addition of SMC-ATP and planting K. paniculata showed a good phytostabilization effect in the manganese slag and further revealed the response process of the fungal community in phytoremediation. [ABSTRACT FROM AUTHOR]

Heavy metals (HMs) contamination, owing to their potential links to various chronic diseases, poses a global threat to agriculture, environment, and human health. Nickel (Ni) is an essential element however, at higher concentration, it is highly phytotoxic, and affects major plant functions. Beneficial roles of plant growth regulators (PGRs) and organic amendments in mitigating the adverse impacts of HM on plant growth has gained the attention of scientific community worldwide. Here, we performed a greenhouse study to investigate the effect of indole-3-acetic acid (IAA @ 10− 5 M) and compost (1% w/w) individually and in combination in sustaining cauliflower growth and yield under Ni stress. In our results, combined application proved significantly better than individual applications in alleviating the adverse effects of Ni on cauliflower as it increased various plant attributes such as plant height (49%), root length (76%), curd height and diameter (68 and 134%), leaf area (75%), transpiration rate (36%), stomatal conductance (104%), water use efficiency (143%), flavonoid and phenolic contents (212 and 133%), soluble sugars and protein contents (202 and 199%), SPAD value (78%), chlorophyll 'a and b' (219 and 208%), carotenoid (335%), and NPK uptake (191, 79 and 92%) as compared to the control. Co-application of IAA and compost reduced Ni-induced electrolyte leakage (64%) and improved the antioxidant activities, including APX (55%), CAT (30%), SOD (43%), POD (55%), while reducing MDA and H2O2 contents (77 and 52%) compared to the control. The combined application also reduced Ni uptake in roots, shoots, and curd by 51, 78 and 72% respectively along with an increased relative production index (78%) as compared to the control. Hence, synergistic application of IAA and compost can mitigate Ni induced adverse impacts on cauliflower growth by immobilizing it in the soil. [ABSTRACT FROM AUTHOR]

Tailings and mine dumps are often pollutant sources that pose serious environmental threats to surrounding areas. The use of pioneer vascular plants to extract or stabilize metals is considered among the more effective mine tailing reclamation techniques. The study aimed at evaluating the phytoremediation potential of Pinus halepensis in abandoned mine-tailing (SW-Sardinia, Italy). Plant ability to tolerate high Zn, Pb, and Cd concentration and their accumulation in roots and aerial parts were assessed at greenhouse conditions. Experiments were performed on 45 seedlings planted in different substrates (mine-tailings, mine-tailings compost-amended, and reference) and on 15 seedlings grown spontaneously in the contaminated mine site investigated with their own substrates. The phytostabilization potential of plant was evaluated through biological accumulation and translocation indexes together with plant survival and biometric parameters. The outcomes showed the adaptability of P. halepensis to grow and survive in contaminated substrates. Compost addition did not improve plant survival and growth, however, it enhanced total carbon and nitrogen contents of soil, restricted metal bioavailability, and accumulation in plant aerial parts. These findings highlight that P. halepensis may be considered for phytostabilization given the great potential to limit Zn, Pb, and Cd toxicity in plant tissues by applying compost amendment in metal contaminated mine sites. The novelty of this study is the selection of Pinus halepensis Mill. as a proper tree species for long-term phytoremediation of multi-heavy metal mine tailing sites. This plant species not only had adaptation to the Mediterranean climate and could tolerate high temperatures and high metal concentrations, but also showed high survival and growth percentage of its roots and epigean organs in highly contaminated mine tailing. Pinus halepensis could limit metal accumulation and toxicity in the aerial part of the plant with the addition of compost amendment. This study demonstrates that compost can enhance soil properties and modify metal bioavailability. The outcomes can be beneficial for the phytostabilization project and restoration of similar Mediterranean mine sites. [ABSTRACT FROM AUTHOR]

Background and aims: Re-vegetation of mining-impacted landscapes reduces transport of toxic elements while improving soil fertility. This study evaluated whether the planting of a native perennial grass with a consortium of diazotrophic microbial endophytes and municipal waste compost—alone and in combination—enhanced plant growth while stabilizing metal(loids) in dolomite-amended tailings from a historically mined polymetallic mineral deposit. Methods: We grew Bouteloua curtipendula seedlings in tailings with hazardous concentrations of As, Cd, Pb, Mn, and Zn. We evaluated how plant growth, organic matter accumulation, and major, minor, and trace element mobilization and phytostabilization responded to microbial endophyte and/or compost amendments after the 45-day growth experiment. Results: Although most of the added endophytes were not uniquely identified, the best plant growth and fertility outcomes were achieved with a combination of amendments: dolomite to reduce acidity, compost to increase nitrogen, and a mixed consortium endophyte seed coating to synergistically increase organic carbon and grass biomass yields. Compost reduced shoot and root concentrations—but not yields—of contaminant metals. Endophytes increased foliar Cd, Co, Mn, and Pb yields but mobilized Pb and Zn from the tailings. Root stabilization of Cd, Co, Mn did not require amendments. Conclusion: The most effective means of revegetating these acidic, polymetallic tailings with the native B. curtipendula is with a simultaneous dolomite, compost, and endophyte seed treatment. Due to potential phosphate solubilization and siderophore production by this consortium of endophytes, strategies to capture solubilized metal(loids) may be needed for sulfidic tailings with metal(loids) associated with mobile mineral phases. [ABSTRACT FROM AUTHOR]

Arsenic (As) is a heavy metal that is toxic to both plants and animals. Silicon nanoparticles (SiNPs) can alleviate the detrimental effects of heavy metals on plants, but the underlying mechanisms remain unclear. The study aims to synthesize SiNPs and reveal how they promote plant health in Arsenic-polluted soil. 0 and 100% v/v SiNPs were applied to soil, and Arsenic 0 and 3.2 g/ml were applied twice. Maize growth was monitored until maturity. Small, irregular, spherical, smooth, and non-agglomerated SiNPs with a peak absorbance of 400 nm were synthesized from Pycreus polystachyos. The SiNPs (100%) assisted in the development of a deep, prolific root structure that aided hydraulic conductance and gave mechanical support to the maize plant under As stress. Thus, there was a 40–50% increase in growth, tripled yield weights, and accelerated flowering, fruiting, and senescence. SiNPs caused immobilization (As(III)=SiNPs) of As in the soil and induced root exudates Phytochelatins (PCs) (desGly-PC2 and Oxidized Glutathione) which may lead to formation of SiNPs=As(III)–PCs complexes and sequestration of As in the plant biomass. Moreover, SiNPs may alleviate Arsenic stress by serving as co-enzymes that activate the antioxidant-defensive mechanisms of the shoot and root. Thus, above 70%, most reactive ROS (OH) were scavenged, which was evident in the reduced MDA content that strengthened the plasma membrane to support selective ion absorption of SiNPs in place of Arsenic. We conclude that SiNPs can alleviate As stress through sequestration with PCs, improve root hydraulic conductance, antioxidant activity, and membrane stability in maize plants, and could be a potential tool to promote heavy metal stress resilience in the field. [ABSTRACT FROM AUTHOR]

Mercury (Hg) pollution has seriously threatened the crop productivity and food security. In the present research, experiments were conducted to assess the influence of nanoscale sulfur/sulfur nanoparticles and the corresponding bulk and ionic sulfur forms on the growth and Hg accumulation of oilseed rape seedlings grown on Hg-contaminated soil, as well as the transformation of soil Hg fractions. The results showed a significant reduction in fresh biomass for seedlings grown on 80–200 mg/kg Hg-polluted soil after 30 days. At 120 mg/kg Hg treatment, 100–300 mg/kg sulfur nanoparticles (SNPs) application counteracted Hg toxicity more effectively compared to the corresponding bulk sulfur particles (BSPs) and ionic sulfur (sulfate) treatments. The seedlings treated with 120 mg/kg Hg + 300 mg/kg SNPs gained 54.2 and 56.9% more shoot and root biomass, respectively, compared to those treated with Hg alone. Meanwhile, 300 mg/kg SNPs application decreased Hg accumulation by 18.9 and 76.5% in shoots and roots, respectively, relative to Hg alone treatment. SNPs treatment caused more Hg to be blocked in the soil and accumulating significantly less Hg in plants as compared to other S forms. The chemical fractions of Hg in the soil were subsequently investigated, and the solubility of Hg was significantly decreased by applying SNPs to the soil. Especially 200–300 mg/kg SNPs treatments caused the ratio of the soluble/exchangeable and the specifically absorbed fraction to be the lowest, accounting for 1.95–4.13% of the total Hg of soil. These findings suggest that adding SNPs to Hg-contaminated soils could be an effective measure for immobilizing soluble Hg and decreasing the Hg concentration in the edible parts of crops. The results of the current study hold promise for the practical application of SNPs to Hg-contaminated farmland for better yields and simultaneously increasing the food safety. The novelty of this study is the selection of oilseed rape and nanoscale sulfur (NS) or sulfur nanoparticles (SNPs) as nontoxic nanomaterial to counteract the Hg toxicity and accumulation. Oilseed rape was selected due to its wide adaptability to various environmental conditions and the high-value oil for human consumption and biofuels production. These advantages make oilseed rape a highly valuable crop for various applications. NS was selected due to its reported ability to limit the uptake of heavy metals in oilseed rape, rice, and wheat along with other crops and subsequently restrict the toxicity of heavy metals in these plants and improve food safety. In this study, we evaluated the growth, Hg accumulation, and the resulting toxicity in oilseed rape grown on Hg-contaminated soil, with or without amendments with NS. The outcomes from this study provided evidence of the significant potential of NS in preventing Hg bioaccumulation and improving crop yields in oilseed rape. This provides opportunity to use NS as an ideal non-GMO approach to limit toxic metals in crops. [ABSTRACT FROM AUTHOR]

Wetland plants play a crucial role in regulating soil geochemistry, influencing heavy metal (HM) speciation, bioavailability, and uptake, thus impacting phytoremediation potential. We hypothesized that variations in HM biogeochemistry within estuarine soils are controlled by distinct estuarine plant species. We evaluated the soils (pH, redox potential, rhizosphere pH, HM total concentration, and geochemical fractionation), plant parts (shoot and root), and iron plaques of three plants growing in an estuary affected by Fe-rich mine tailings. Though the integration of multiple plant and soil analysis, this work emphasizes the importance of considering geochemical pools of HM for predicting their fate. Apart from the predominance of HM associated with Fe oxides, Typha domingensis accumulated the highest Cr and Ni contents in their shoots (> 100 mg kg−1). In contrast, Hibiscus tiliaceus accumulated more Cu and Pb in their roots (> 50 mg kg−1). The differences in rhizosphere soil conditions and root bioturbation explained the different potentials between the plants by altering the soil dynamics and HM’s bioavailability, ultimately affecting their uptake. This study suggests that Eleocharis acutangula is not suitable for phytoextraction or phytostabilization, whereas Typha domingensis shows potential for Cr and Ni phytoextraction. In addition, we first showed Hibiscus tiliaceus as a promising wood species for Cu and Pb phytostabilization.

Although the phytoremediation strategy has been studied worldwide, little research data are available regarding the influence of mycorrhizae on the phytoremediation capacity of various plants grown in Cd-contaminated soils in Mediterranean environments. Therefore, a pot experiment was carried out to study the possible effectiveness of hemp plant (Cannabis sativa L.) in the remediation of moderately and heavily Cd-contaminated soils and additionally to quantify the effect of Cd on Arbuscular Mycorrhizal Fungi (AMFs). For this purpose, an alkaline clay soil collected from the Farm of Institute of Plant Breeding and Genetic Resources (North Greece) was contaminated with two levels of Cd (3 and 30 mg Cd kg−1, corresponding to Levels A and B, respectively—first factor) at two incubation times (10 and 30 days—second factor) and six treatments (Control_30d, Control_10d, CdA_30d, CdB_30d, CdA_10d, CdB_10d) were created. Soil Cd concentrations, both pseudo-total and available to plants, were determined after extraction with Aqua Regia mixture and DTPA solution, respectively, before and after the cultivation of hemp plants and after the harvesting. Cd concentrations in the aboveground and underground plant parts were also estimated after digestion with Aqua Regia, while root colonization by AMFs was determined with a microscope. The highest plant’s Cd concentration, more than 50%, was observed in its underground part, at all Cd-contaminated treatments, indicating a strong capacity for cadmium to gather up in the roots. Among different Cd levels and incubation days, significant differences were recorded in the rates of root colonization by AMFs. Among different Cd levels and incubation days, 3 mg Cd Kg−1 soil promoted AMF root colonization, particularly at 10-day incubation, while 30 mg Cd Kg−1 soil diminished it. Colonization was lower with longer incubation times at both levels of Cd. Hemp appears to be a viable option for phytostabilization in Cd-contaminated soils, enabling further utilization of AMFs to assist the phytoremediation process.

The increasing demand for mineral resources has generated mine tailings with heavy metals (HM) that negatively impact human and ecosystem health. Therefore, it is necessary to implement strategies that promote the immobilization or elimination of HM, like phytoremediation. However, the toxic effect of metals may affect plant establishment, growth, and fitness, reducing phytoremediation efficiency. Therefore, adding organic amendments to mine tailings, such as biochar, can favor the establishment of plants, reducing the bioavailability of HM and its subsequent incorporation into the food chain. Here, we evaluated HM bioaccumulation, biomass, morphological characters, chlorophyll content, and genotoxic damage in the herbaceous Crotalaria pumila to assess its potential for phytostabilization of HM in mine tailings. The study was carried out for 100 days on plants developed under greenhouse conditions under two treatments (tailing substrate and 75% tailing/25% coconut fiber biochar substrate); every 25 days, 12 plants were selected per treatment. C. pumila registered the following bioaccumulation patterns: Pb > Zn > Cu > Cd in root and in leaf tissues. Furthermore, the results showed that individuals that grew on mine tailing substrate bioaccumulated many times more metals (Zn: 2.1, Cu: 1.8, Cd: 5.0, Pb: 3.0) and showed higher genetic damage levels (1.5 times higher) compared to individuals grown on mine tailing substrate with biochar. In contrast, individuals grown on mine tailing substrate with biochar documented higher chlorophyll a and b content (1.1 times more, for both), as well as higher biomass (1.5 times more). Therefore, adding coconut fiber biochar to mine tailing has a positive effect on the establishment and development of C. pumila individuals with the potential to phytoextract and phytostabilize HM from polluted soils. Our results suggest that the binomial hyperaccumulator plant in combination with this particular biochar is an excellent system to phytostabilize soils contaminated with HM.

Soil contaminated with heavy metals cause serious threat to the soil quality, biota, and human. The removal or stabilization of heavy metals through plants is an environment friendly approach. The aim of study was to assess the potential of Celosia argentea L. for the phytoremediation of heavy metals contaminated soil. Soil was spiked with different levels (0, 100, 200, 300, and 400 mg/kg) of chromium (Cr), copper (Cu), lead (Pb), and Zn (Zn). Experiment was carried out in greenhouse and impact of heavy metals was evaluated on plant by assessing the germination rate and plant growth. To evaluate either plant has potential to extract/stabilize the heavy metals, concentration in roots and shoot, translocation factor (TF), bioconcentration factor (BCF), and bioaccumulation factor (BAF) were determined. Application of heavy metals significantly affected the germination rate and minimum (26.6%) was observed in Cr spiked soil (400 mg/kg). Moreover, the biomass of C. argentea was also affected by the application of heavy metals. However, the concentration of heavy metals in roots and shoots were low. The BCF and BAF of C. argentea was lower than 1 except at lower levels of Pb and Zn, but the TF was greater than 1. The TF showed that plants have capability to transfer heavy metals to shoots once they are taken up by roots. However, based on the BCF and concentrations of heavy metals in shoots, it is evident that plant could play important role in the phytostabilization of heavy metals polluted soil. [ABSTRACT FROM AUTHOR]

The influence of sowing cereal-legume mixtures and application of mineral fertilizers on technozem created after the liquidation of the tailing dump of the Dzhida Tungsten-Molybdenum Works (Republic of Buryatia) on the change of trace element concentrations in plants and formation of sod limiting the spread of pollutants and reducing environmental risks was assessed. The content of total and mobile forms of some trace elements in the upper loamy sand layer of technozem was higher than in the background soil, exceeded the median background for the soils of Transbaikalia and in some cases the maximum permissible concentrations, and was characterized as moderately hazardous by the coefficient of total contamination (Zc = 18.8); the lower loamy layer was nonhazardous (Zc = 4). It was revealed that the application of fertilizers reduced the concentration of trace elements and their accumulation coefficients in plants. According to the intensity of biological uptake, most of the elements in the aboveground phytomass belonged to the group of medium capture; in the underground phytomass, to the group of medium and intense uptake, which indicates its phytostabilization role. Bioproductivity of cereal–legume mixtures in the control was low. Fertilizer application increased the bioproductivity of the mixtures year to the moderate level on the second year and to the high level on the third year; the dense sod layer fixing the surface and contributing to the increase in the soil organic matter content in comparison with its initial amount was formed on the fourth year. The results of this study can be applied in remediation works on overburden dumps with the creation of technozems for phytostabilization and initiation of organic matter accumulation in these soils by sowing high-yielding perennial herbs and applying mineral fertilizers. [ABSTRACT FROM AUTHOR]

Human activities can cause enormous damage to agricultural soils through the accumulation of toxic metals in the soil. The identification of plants capable of accumulating relatively large amounts of these compounds in plant tissues with the aim of reducing or limiting soil toxicity is of great interest. Two independent pot experiments were conducted to evaluate the phytoremediation potential of different varieties of oat (Avena sativa L.) grown in soil contaminated with cadmium (Cd, 50 mg kg-1s soil). Eight varieties were cultivated for 21 days in Cd- contaminated soil. Five varieties of oat were exposed to Cd at the third leaf stage, followed one week later by exposure to oat powdery mildew (Blumeria graminis f. sp. avenae) for 35 days. In general, the tested varieties accumulated more Cd in the roots than in the shoots and showed a high tolerance to Cd. Metal accumulation in shoots was lower after 21 days of cultivation (8.30-17.27 mg Cd kg-1) than after 42 days (13.72-35.20 mg Cd kg-1) and was the highest in tissues of plants infected with B. graminis (48.17-96.20 mg kg-1). In conclusion, our results indicate a good phytostabilization and remediation potential of oat (varieties Racoon and Vaclav) for soil contaminated with cadmium. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) is one of the most common toxic heavy metal elements in soil pollution, which can be continuously enriched in the food chain and eventually threaten human health. Phytoremediation, which is using plants to transfer heavy metal elements from soils, is a promising solution for the remediation of heavy metal-contaminated soils. In this study, we evaluated whether Cunninghamia lanceolata (Lambert) Hooker (Chinese fir), a widely planted timber tree worldwide, had the potential to remediate Cd-contaminated soils through 90 days pot of experiments with different Cd concentration soils (0, 5, 10, 20, 50, 100 mg kg−1). C. lanceolata did not show obvious toxic symptoms in Cd-contaminated soils, although Cd inhibited plant growth and decreased net photosynthetic rate slightly. The activities of antioxidant enzymes increased significantly under Cd stress, indicating that C. lanceolata had a strong self-regulation ability and can tolerate Cd stress. The Cd bioconcentration factor (Cd concentration in plant divided by Cd concentration in soil) of C. lanceolata were greater than 1 at all Cd concentrations, indicating that C. lanceolata had a strong ability to absorb Cd, although Cd was mainly accumulated in roots. Our results indicated that C. lanceolata had a strong tolerance and phytostabilization ability of Cd. Considering the wide distribution worldwide, large biomass, and rapid growth of C. lanceolata, it could be a promising candidate for phytoremediation of Cd-contaminated soils. [ABSTRACT FROM AUTHOR]

Exogenous addition of IAA has the potential to improve the metal tolerance and phytostabilization of plants, but these effects have not been systematically investigated in naturally tolerant plants. Ryegrass (Lolium perenne L.) is a typical indigenous plant in the Lanping Pb/Zn mining area with high adaptability. This study investigated the phytostabilization ability and Pb tolerance mechanism of ryegrass in response to Pb, with or without foliar spraying of 0.1 mmol L-1 IAA. The results indicated that appropriate IAA treatment could be used to enhance the phytostabilization efficiency of naturally tolerant plants. Foliar spraying of IAA increased the aboveground and belowground biomass of ryegrass and improved root Pb phytostabilization. Compared to Pb-treated plants without exogenous IAA addition, Pb concentration in the shoots of ryegrass significantly decreased, then increased in the roots after the foliar spraying of IAA. In the 1,000 mg kg-1 Pb-treated plants, Pb concentration in the shoots decreased by 69.9% and increased by 79.1% in the roots after IAA treatment. IAA improved plant growth, especially in soils with higher Pb concentration. Foliar spraying of IAA increased shoot biomass by 35.9% and root biomass by 109.4% in 1,000 mg kg-1 Pb-treated plants, and increased shoot biomass by 196.5% and root biomass by 71.5% in 2,000 mg kg-1 Pb-treated plants. In addition, Pb stress significantly decreased the content of photosynthetic pigments and anti-oxidase activities in ryegrass, while foliar spraying of IAA remedied these negative impacts. In summary, foliar spraying of IAA could increase the biomass and improve the Pb tolerance of ryegrass. [ABSTRACT FROM AUTHOR]

Phytoremediation, an environmentally friendly and sustainable approach for addressing Cu-contaminated environments, remains underutilized in mine tailings. Arbuscular mycorrhizal fungi (AMF) play a vital role in reducing Cu levels in plants through various mechanisms, including glomalin stabilization, immobilization within fungal structures, and enhancing plant tolerance to oxidative stress. Yeasts also contribute to plant growth and metal tolerance by producing phytohormones, solubilizing phosphates, generating exopolysaccharides, and facilitating AMF colonization. This study aimed to assess the impact of AMF and yeast inoculation on the growth and antioxidant response of Oenothera picensis plants growing in Cu mine tailings amended with compost. Plants were either non-inoculated (NY) or inoculated with Meyerozyma guilliermondii (MG), Rhodotorula mucilaginosa (RM), or a combination of both (MIX). Plants were also inoculated with Claroideoglomus claroideum (CC), while others remained non-AMF inoculated (NM). The results indicated significantly higher shoot biomass in the MG-NM treatment, showing a 3.4-fold increase compared to the NY-NM treatment. The MG-CC treatment exhibited the most substantial increase in root biomass, reaching 5-fold that in the NY-NM treatment. Co-inoculation of AMF and yeast influenced antioxidant activity, particularly catalase and ascorbate peroxidase. Furthermore, AMF and yeast inoculation individually led to a 2-fold decrease in total phenols in the roots. Yeast inoculation notably reduced non-enzymatic antioxidant activity in the ABTS and CUPRAC assays. Both AMF and yeast inoculation promoted the production of photosynthetic pigments, further emphasizing their importance in phytoremediation programs for mine tailings. [ABSTRACT FROM AUTHOR]

The study of the rhizospheric microbiome in native plants should be a prerequisite before carrying out the phytomanagement of mine tailings. The goal of this work was to evaluate the rhizospheric microbiome of Piptatherum miliaceum in semiarid mine tailings. A comprehensive edaphic characterization was performed including the description of soil microbial composition in the rhizosphere of P. miliaceum growing at a mine tailings pile and at a control site. Plant nutritional and isotopic compositions were also determined. Neutral pH of the tailings (7.3) determined low metal extractability in 0.01 M CaCl2 (e.g. < 1 mg/kg for Zn). In spite of the contrasting edaphic fertility conditions of both sites, N (~ 15 g kg−1) and P (~ 400 mg kg−1) leaf concentrations were similar. The lower δ15N at the tailings plants (− 4.50‰) compared to the control (6.42‰) indicated greater efficiency of P. miliaceum for uptaking N under the low fertility conditions of the tailings (0.1% total soil nitrogen). The presence at the tailings of bacterial orders related to the cycling of N, such as Rhizobiales, could have contributed to enhance N acquisition. The lower leaf δ13C values at the tailings (− 30.22‰) compared to the control (− 28.47‰) indicated lower water use efficiency of the tailing plants. Some organotrophic bacterial and fungal groups in the tailings' rhizospheres were also found in the control site (e.g. Cytophagales, Sphingobacteriales for bacteria; Hypocreales, Pleosporales for fungi). This may indicate that P. miliaceum is able to shape its own specific microbiome at the tailings independently from the initial microbial composition of the tailings. [ABSTRACT FROM AUTHOR]

Lead (Pb) is one of the most dreadful non-essential elements whose toxicity has been well reported worldwide due to its interference with the major plant functions and its overall yield. Bioremediation techniques comprising the application of beneficial microorganisms have gained attention in recent times owing to their ecofriendly nature. Addition of organic matter to soil has been reported to stimulate microbial activities. Compost application improves soil structure and binds toxic contaminants due to its larger surface area and presence of functional groups. Furthermore, it stimulates soil microbial activities by acting as C-source. So, in current study, we investigated the individual and synergistic potential of two lead (Pb)-tolerant Pseudomonas strains alongwith compost (1% w/w) in sustaining sunflower growth under Pb contaminated soil conditions. Lead chloride (PbCl2) salt was used for raising desired Pb concentration (500 mg kg−1). Results revealed that Pb stress drastically affected all the measured attributes of sunflower plant, however joint application of rhizobacteria and compost counteracted these adverse effects. Among them, co-application of str-1 and compost proved to be significantly better than str-2, as its inoculation significantly improved shoot and root lengths (64 and 76%), leaf area and leaves plant−1 (95 and 166%), 100-achene weight (200%), no. of flowers plant−1 (138%), chl ‘a’, ‘b’ and carotenoid (86, 159 and 33%) contents in sunflower as compared to control treatments. Furthermore, inoculation of Pseudomonas fluorescens along with compost increased the NPK in achene (139, 200 and 165%), flavonoid and phenolic contents (258 and 185%) along with transpiration and photosynthetic rates (54 and 72%) in leaves as compared to control treatment under Pb contamination. In addition, Pb entry to roots, shoots and achene were significantly suppressed under by 87, 90 and 91% respectively due to integrated application of compost and str-1 as evident by maximum Pb-immobilization efficiency (97%) obtained in this treatment. Similarly, bioconcentration factors for roots, shoots and achene were found to be 0.58, 0.18 and 0.0055 with associated translocation factor (0.30), which also revealed phytostabilization of Pb under combined application of PGPR and compost. Since, phytoremediation of heavy metals under current scenario of increasing global population is inevitable, results of the current study concluded that tolerant PGPR species along with organic amendments such as compost can inhibit Pb uptake by sunflower and confer Pb tolerance via improved nutrient uptake, physiology, antioxidative defense and gas exchange.

In this study, novel Schiff base complexes with Zn(II) and Co(II) ions were successfully synthesized. The malonic acid dihydrazide was converted into the Schiff base ligand by combining it with 1-hydroxy-2-naphthaldehyde, and the last step required reacting it with the appropriate metal(II) chloride to produce pure target complexes. The generated complexes were thoroughly characterized using FTIR, 1H-NMR, 13C-NMR, GC-mass, and UV-Vis spectroscopies. In order to photo-stabilize polystyrene (PS) and reduce the photodegradation of its polymeric chains, these chemicals have been used in this work. The efficiency of the generated complexes as photo-stabilizers was evaluated using a variety of techniques, including FTIR, weight loss, viscosity average molecular weight, light and atomic force microscopy, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) mapping. These tests corroborated each other and demonstrated how effectively new compounds stabilize PS photographs. As a result, compared to blank PS, they reduce the photodegradation of PS films containing these complexes after 300 h of exposure to UV radiation with a wavelength of 313 nm. Also, it has demonstrated how effective the cobalt complex is as a photo-stabilizer. The highly conjugated systems in these chemicals are to blame for this.

Several mining-related pollutions, industrial waste, and soil deterioration define South Africa’s environmental landscape. These have led to the consumption of unhealthy food, contaminated agricultural products, and polluted water. The polluted environment has been linked to numerous diseases among the populace, thus making environmental remediation an important issue in South Africa. Phytoremediation has been identified as a biological method for the restoration of polluted environments naturally and holistically. Therefore, it is vital to evaluate the level of phytoremediation-related research in South Africa in pursuit of a way out of environmental pollution. Thus, the purpose of this study was to map phytoremediation-related research in South Africa from inception to 2022. Statistical records from the Web of Science Core Collection were analyzed with the bibliometric package in RStudio, while mapping was performed via VOSviewer. Our study showed a low annual growth rate of publication (4.49%). The analysis uncovered that the 39 documents analyzed were written by 112 authors, and the first document was featured in the Journal of Geochemical Exploration in 1997. Kirkham, MB and Liphadzi, MS are the most relevant authors. USA has the strongest collaboration with South Africa, while the International Journal of Phytoremediation, the South African Journal of Botany, and Water SA are the most relevant journals. The result of this study can guide upcoming researchers and policymakers, together with essential facts for enhancing the restoration of the polluted environment in the country.