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Srivilliputhur Wildlife Sanctuary holds significant value being a part of Western Ghats, which remains unexplored in terms of vegetation. The current study is the first quantitative assessment of tree distribution along an elevation gradient combined with environmental variables. Sampling was conducted from December 2020 to April 2021 in six elevation zones (Z1 to Z6). A total of 135 plots of 20m × 20m size were established, and the stems were measured (>30 cm GBH). Across a 5.4 ha studied area, 2157 individuals representing 188 tree species and 48 families were documented. Tree density (348-473 ind. ha−1), basal area (21.71-36.59 m2 ha−1), and species richness (84-122) were highest at mid-elevations. One-way ANOVA result highlighted the significant influence of elevation on species richness (p

Local and illegal exploitation of wildlife is particularly widespread in highly populated and bio-diverse countries such as India. In such countries, illicit utilization practices and how these are perceived among local communities are commonly not well-documented. Due to a lack of data, and environmental education measures to protect ecosystems and biodiversity, the extent of this exploitation appears immense and often goes unnoticed. Therefore, in 2019, a series of awareness workshops targeting the conservation of wildlife focused on monitor lizards (Varanidae) was conducted in five districts of West Bengal, India. The environmental awareness workshops were conducted through PowerPoint slide presentations and questionnaires collecting baseline data from the participants regarding their perceptions and relationships to the three species of Varanus recorded for West Bengal. Our findings reveal specific human attitudes and varying local utilization patterns of monitor lizards. This study was the first to communicate and spread awareness in the study areas about why the conservation of local wildlife such as Varanus species is important for ecosystems and human well-being based on the ‘Nature Contributions to People Concept’.

Abstract The integration of biomolecules into supramolecular nanostructures forms the basis of the natural world. Naturally occurring liquid-liquid phase separation resulting in biomolecular condensates has inspired the formation of biomolecule-based smart materials with multi-dimensional applications. A non-covalent bio-condensation between biomass DNA and guanosine monophosphate (GMP) has been described, mimicking chromatin folding and creating a unique “all-nucleic” DNA-GMP condensates. These condensates initiate the formation of G-quadruplex-based superstructures, assembling into super-helical fibres driven by synergistic hydrogen bonding and stacking, which have been thoroughly investigated. This simple, one-step method for the bio-condensation of biomass DNA leads to an “all-nucleic” hydrogel with higher-order self-assembly and excellent mechanical properties. While most of the reported DNA based biomaterials, including hydrogels, require precisely sequenced and molecularly architectured DNA building blocks, we have developed a simple, universal, and facile bio-condensation method that utilizes biomass DNA acquired from any bio-resource to fabricate DNA hydrogels. The hydrogel efficiently encapsulates and sustains the release of both hydrophilic and hydrophobic drugs, demonstrating its competency as a drug carrier. We believe this energy-efficient and low-cost method represents a new technique for using biomass DNA as building blocks for the next generation of soft materials.

Abstract Alzheimer’s disease (AD), a progressive neurodegenerative disorder, is characterized by neurological impairments such as visual and sensory difficulties, motor dysfunction, sphincter issues, incoordination, gait abnormalities, and cognitive decline. Despite advances in understanding AD pathophysiology and the expansion of therapeutic options over the past three decades, the disease remains incurable. Current therapies, even those specifically targeting AD, often fail to significantly alter its progression, underscoring the need for innovative treatment approaches beyond symptomatic relief. This calls for a re-examination of AD pathology to identify potential therapeutic targets that go beyond conventional strategies. This review highlights four of the most promising non-canonical therapeutic targets: oligodendrocytes, the blood–brain barrier (BBB), neuroimmunometabolism, and the coagulation system. These components are crucial for maintaining the integrity and proper function of neurons and the brain, playing key roles in the progression of AD. Oligodendrocytes, for example, are essential for myelination and neuronal support, while BBB dysfunction can lead to impaired clearance of toxic proteins. Neuroimmunometabolism offers insights into how metabolic processes influence immune responses in the brain and dysregulation of the coagulation system has been linked to increased neuroinflammation and vascular abnormalities in AD. Recent discoveries in these fields provide new avenues for understanding the disease and identifying potential therapeutic targets. By exploring these non-canonical pathways, future research may offer breakthroughs in treating AD, moving beyond symptomatic management towards disease-modifying strategies.

Abstract In this article, the potential Kadomtsev-Petviashvili (pKP) type coupled system with variable coefficients is studied, which have many applications in wave phenomena and soliton interactions in a two-dimensional space with time. In this framework, Hirota bilinear form is applied to acquire diverse types of interaction lump solutions from the foresaid equation. Abundant lump, lump-periodic, lump-soliton and multi soliton solutions to the pKP system are presented by the Hirota bilinear form and a mixture of exponentials and trigonometric functions. Lump, lump-periodic, lump-soliton and multi soliton solutions are studied with the usage of symbolic computation. In addition, the symbolic computation and the applied methods for governing model are investigated. The movement role of the waves is investigated, and the theoretical analysis of the acquired solutions is discussed using the bilinear technique of all produced solutions with 2D and 3D plots with respective parameters. The computational difficulties and outcomes highlight the clarity, effectiveness, and simplicity of the approaches, suggesting that these schemes can be applied to a variety of dynamic and static nonlinear equations governing evolutionary phenomena in computational physics as well as to other real-world situations and a wide range of academic fields. We used software Maple 2024 “( http://www.maplesoft.com/ )”.

Abstract Numerous neurological disorders are linked to sequences rich in guanine repeats found in introns, exons, and regulatory regions of genes. These sequences have been observed to form stable G-quadruplex (GQ) structures both in vitro and in vitro. Cerebellar Ataxia with Neuropathy and Vestibular Areflexia Syndrome (CANVAS), a slowly progressive neurodegenerative disorder, is associated with the biallelic expansion of (AAGGG)n pathogenic repeats in the second intron of the RFC1 gene. Though these G-rich pathogenic repeats in other neurological diseases are associated with protein loss of function, RNA gain of function, and/or protein gain of function, not much is known about the pathological mechanism associated with CANVAS. Herein, we report the formation of stable GQ conformations in the CANVAS-associated repeats i.e., r(AAGGG)n, where ‘r’ stands for RNA. These GQs are critical regulators in neurological disorders leading to RNA foci formation and RNA binding protein sequestration. They also alter other causative processes like intron retention, which leads us to hypothesize a toxic Proteinopathy mechanism in CANVAS. Various biophysical and biomolecular assays characterized the interactions of three aggregation-prone RNA-binding proteins (RBPs): heterogeneous nuclear ribonucleoprotein H1/F (hnRNP H1/F), and DGCR8 with different pathogenic repeats [(AAGGG)9] in vitro, further affirming the hypothesis. The biophysical observations are further supported by molecular dynamics analysis and cell-based studies, putting us a step closer to elucidating the pathological mechanism(s) in CANVAS neuropathy, paving the way for the development of innovative therapeutic interventions.

The narratives around devadasi are historically constructed to glorify the dedication of young girls as dancers to various temples across India. Traditionally, a devadasi assumed a pivotal role in executing significant rites and festivities within temple precincts, thereby representing an indispensable contributor to the cultural milieu inherent to these sacred edifices. This side of the devadasi legacy is quite popular and known even in the present times, but a discreet silence prevails about the flip side of this picture, a side that is characterised by systemic oppression, exploitation, and enduring bondage, constituting a narrative often hushed up. The present paper investigates the profoundly complex and concealed aspects of the devadasi tradition through a study of narratives by Gogu Shyamala, William Darlymple and Sudha Murthy using a post-truth lens and Baudrillardâs âsimulacraâ to deconstruct the coordinated distortion/asymmetry of/in knowledge/reality of devadasi.

Abstract The crude leaf extract of Phragmanthera macrosolen L. has been utilized for the first time as an effective reducing, capping and stabilizing agent to synthesize silver oxide nanoparticles (Ag2O NPs) through a green approach. The prepared Ag2O NPs were analyzed by scanning electron-microscopy (SEM), High Resolution Transmission electron microscope (HR-TEM), X-ray diffractions (XRD), Fourier transforms infrared (FTIR) spectroscopy, energy dispersive spectroscopy (EDS), and Ultra-violet visible spectrometry (UV-Vis). The biosynthesized Ag2O NPs applied on gram-positive (S. aureus) and gram-negative (E. coli) bacterial types. FTIR spectral peaks indicate that the phytochemicals in the extract are responsible for the formation of Ag2O nanoparticles. The XRD result shown, poly-crystalline nanoparticles and the average crystalline size calculated was 45.8 nm. UV-Vis analysis shows absorbance existed at 590.5 nm, and the energy band gap calculated through the Tauc relation was 2.1 eV. The SEM images gave a globular and some rod like morphology with diameter of particle obtained between 20.11 and 46.50 nm with some hollow cubic microstructure of Ag2O NPs which makes it suitable for antimicrobial application. The EDS confirms the elemental composition and existence of Ag and O2. The HR-TEM images, specific area electron diffraction (SAED), and XRD patterns confirmed the morphology of Ag2O NPs mean 45.84 nm and polycrystalline nature of the nanoparticles. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were 15 and 30 µl respectively for the samples tested. In this study, it was observed that Ag2O NPs highly sensitive to E. coli than s. aureus. The present study revealed that the possible use of green synthesized Ag2O NPs as potential antibacterial agent.

Abstract Here, Er/Yb Co-doped CaMoO4 materials (ErxYbyCa1−x−yMoO4 NPs where x = 0, 0.01 and y = 0, 0.05, 0.10, 0.15, 0.20) were prepared by microwave-assisted method and its pure-tetragonal structure were confirmed by X-ray diffraction spectra (XRD), Rietveld-refinement and Raman-vibrational spectra. The transmission-electron microscopy (TEM) study indicates the formation of nearly spherical shaped nanomaterial with average size of ~ 15 nm. Additionally, absorption and emission properties were studied by UV–Visible–NIR and photoluminescence (PL) spectrometer. The UV–Visible–NIR spectra attribute absorption of Er3+ ion in visible region (380–700 nm) and absorption of Yb3+ ion in near-infrared region (700–1400 nm). Moreover, PL up-conversion spectra of the sample recorded under excitation wavelength 980 nm. The PL peaks were observed at 530, 544–552, and 656–670 nm. Prominent PL-intensity was observed for Er0.01Yb0.15Ca0.84MoO4 phosphor. Temperature dependent PL study reveals that present phosphor is robust phosphor for temperature sensor. In addition, Er3+/Yb3+ doped CaMoO4 nanomaterials exhibited excellent activity and selectivity in the aerial oxidation of benzoin over benzyl under oxidant-free reaction conditions. The synergistic effect Er3+/Yb3+ co-doing in CaMoO4 matrix was observed, where only CaMoO4 material found to be inactive towards above oxidation reaction. Moreover, the oxidation of primary benzyl-alcohols was furnished in presence of tertiary butyl hydroperoxideas oxidant.

Abstract This study presents nanostructured composite Bi2MoO6/MXene heterostructure by using hydrothermal method for photodegradation of the congo-red dye and also for energy storage devices. X-ray diffractometer (XRD), High Resolution Transmission Electron Microscopy (HRTEM), Field emission scanning electron microscope (FESEM) and X-ray photoelectron spectroscopy (XPS), Brunauer-Emmett-Teller (BET) were performed to examine the structural properties along with surface area and porosity of the material. Due to addition of MXene the larger surface area and improved pore size help to quickly break down additional organic pollutants by adsorbing them. The band gap of Bi2MoO6/MXene nanostructured composite reduced to 2.4 eV suggesting transfer of electrons from VB to CB. Bi2MoO6/MXene exhibits a high (92.3%) photocatalytic degradation rate for a duration of 16 min which was verified using UV-visible spectroscopy, also scavenger test was conducted to ascertain the reactive agent along with the degradation pathway was confirmed by LCMS. Elemental content was also established by using inductively coupled plasma mass spectrometry (ICP-MS). For estimating energy storage capacity cyclic voltammetry (CV) was performed. It was observed Bi2MoO6/MXene nanostructured composite electrodes had specific capacitance of 642.91Fg− 1, power density of 1.24 kWkg− 1, and energy density of 22.32 Whkg− 1 at a current density of 5Ag− 1 also it exhibited 64.42% capacity retention having current density 20 Ag− 1 throughout 10,000 Galvanostatic charge discharge (GCD) cycles. High electrical conductivity of Bi2MoO6/MXene electrode was again examined by Electrochemical impedance spectroscopy (EIS). These findings demonstrate the potential of Bi2MoO6/MXene nanostructured composites in both photodegradation and energy storage applications.

Abstract Fuchs Endothelial Corneal Dystrophy (FECD) is an aging disorder characterized by expedited loss of corneal endothelial cells (CEnCs) and heightened DNA damage compared to normal CEnCs. We previously established that ultraviolet-A (UVA) light causes DNA damage and leads to FECD phenotype in a non-genetic mouse model. Here, we demonstrate that acute treatment with chemical stressor, menadione, or physiological stressors, UVA, and catechol estrogen (4-OHE2), results in an early and increased activation of ATM-mediated DNA damage response in FECD compared to normal CEnCs. Acute stress with UVA and 4OHE2 causes (i) greater cell-cycle arrest and DNA repair in G2/M phase, and (ii) greater cytoprotective senescence in NQO1 −/− compared to NQO1 +/+ cells, which was reversed upon ATM inhibition. Chronic stress with UVA and 4OHE2 results in ATM-driven cell-cycle arrest in G0/G1 phase, reduced DNA repair, and cytotoxic senescence, due to sustained damage. Likewise, UVA-induced cell-cycle reentry, gamma-H2AX foci, and senescence-associated heterochromatin were reduced in Atm-null mice. Remarkably, inhibiting ATM activation with KU-55933 restored DNA repair in G2/M phase and attenuated senescence in chronic cellular model of FECD lacking NQO1. This study provides insights into understanding the pivotal role of ATM in regulating cell-cycle, DNA repair, and senescence, in oxidative-stress disorders like FECD.

Background Ingrown toenail is a nail disorder that commonly affects great toenails. Surgical modalities are commonly used as first-line treatment. A conservative approach is preferable as surgical procedures lead to pain and affect routine activities. Our aim is to study the efficacy of a new conservative technique using steri-stripTM for the treatment of mild to moderate ingrown toenails as an outpatient procedure. Methods Fifty patients presenting with stage 1–2 ingrown toenails were treated with the ‘steri-strip sling technique’. Steri-stripTM is inserted between the disto-lateral edge of the nail plate and nail fold and secured with an elastic bandage. The dressing is kept till the nail edge grows over the nail fold and stops penetrating the skin. Patients were reviewed every week for a month after the treatment and monthly thereafter. The final result was assessed at 6 months. The success of treatment was assessed clinically and by patient satisfaction levels. Results Fifty patients were treated and followed up. Immediately after the procedure, all patients reported significant relief in pain. After 6 months, 46 out of 50 patients were satisfied with the treatment and showed no recurrence. No complications were reported. Conclusion The ‘Steri-stripTM sling technique’ is a simple and effective outpatient procedure for the management of mild to moderate ingrown toenails.

Background: Pleural effusions are classified as exudate or transudate to guide clinical management. While Light’s criteria have been the standard for this differentiation due to their high sensitivity, their lower specificity can lead to potential misclassification of transudates as exudates. This study evaluates the clinical utility of pleural fluid cholesterol compared to Light’s criteria. Aims and Objectives: The aim of the study was to assess the clinical utility of pleural fluid cholesterol as a laboratory parameter in differentiating exudate from transudate pleural effusions. Materials and Methods: This cross-sectional study included 100 participants with pleural effusion. After baseline data collection and clinical examination, blood tests and pleural fluid analyses were conducted. McNemar’s test compared the sensitivity, specificity, and diagnostic accuracy of pleural fluid cholesterol with Light’s criteria. Results: Light’s criteria showed a sensitivity of 98.39%, specificity of 84.21%, positive predictive value (PPV) of 91.04%, negative predictive value (NPV) of 96.97%, and diagnostic accuracy of 93%. Pleural fluid cholesterol (threshold >45 mg/dL) demonstrated a sensitivity of 91.94%, specificity of 97.37%, PPV of 98.28%, NPV of 88.10%, and diagnostic accuracy of 94%. The difference in specificity was statistically significant (P

In this paper, the generalized (3+1)-dimensional nonlinear wave equation in fluid with gas bubbles is studied in soliton theory and produced by taking the Hirota bilinear operators. The first- to third rogue wave solutions through numerous rogue wave strategy by Maple typical estimations are recovered. The made conditions for the analyticity and positively of the gotten arrangements can be effectively accomplished by utilizing the specific determinations of the included values. The most merits of this plot are to recoup the Hirota bilinear models and their generalized equivalences. Also, the rational tan(Π(ξ)) technique on the generalized nonlinear wave equation is examined. The perturbed solution of the generalized nonlinear wave equation through the way of Modulational stability is studied. Finally, the graphical reenactments of the precise arrangements are portrayed. By selecting suitable values for the parameters involved in the solutions, 3D graphs, 2D contour graphs and line graphs are presented to provide graphical demonstration of the results. The reported results will be helpful to design new and better optical devices. The findings of this work may also help in problems arising in ocean engineering.

Context Tobacco addiction is an important public health problem. Among several theories of addiction proposed, PRIME theory of motivation is found to be a comprehensive one to synthesise prevention strategies. Aims This case study was conducted on the basis of PRIME theory to explain smokeless tobacco (SLT) addiction patterns among slum dwelling female residents of Cuttack district, Odisha. Settings and Design Cuttack district of Odisha state and Qualitative synthesis. Methods and Material Qualitative semistructured face-to-face interviews and framework analysis techniques were employed. The study was conducted over a period of 2 months between February 2020 and March 2020. Face-to-face interviews of 26 participants of Dasha Sahi slum using a semistructured questionnaire assessing responses based on five main themes including P, Plans; R, Responses; I, Impulses And Inhibitory Forces; M, Motives; and E, Evaluations of the said theory. Statistical Analysis Used Verbatim transcripts were analysed using thematic framework analysis. Results A majority of the participants were multiple SLT product users, and they did not have an immediate plan to quit. Most of the participants showed strong emotional attachment with these products and considered them the only source of pleasure (want) in a life full of miseries. The words “Tobacco” and “Nicotine” were medical jargons for them. Though they were aware of the harmful effects, they had a positive evaluative belief about its use because of cognitive dissonance. Most of them were habitual users living in an environment full of physical and social cues. Conclusions This case study is well in line with the PRIME theory in explaining the addictive behaviour; a comprehensive multi-strategic approach was formulated for inculcating quitting behaviour among this disadvantaged section of the population as suggested by the proposer of this theory.

Background Human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) coinfection has emerged as a leading cause of morbidity throughout the world in the last two decades. The coronavirus disease 2019 (COVID-19) pandemic has escalated the disease burden further by increasing the number of intravenous (IV) drug abusers and unemployment. Aim The present study was done to analyse the impact of COVID-19 on seroprevalence as well as trends during pre, post and pandemic years of coinfection and mono-infections in the Malwa region of Punjab. Setting and Design This descriptive cross-sectional study was done in the department of immunohematology and blood transfusion at a tertiary care hospital. Materials and Methods The data on transfusion-transmitted infections (TTIs) was collected for a period of four years from 2019 to 2022, that is, pre, post and during the pandemic period. All the blood samples were screened for viral markers, HIV I and II, HCV, and hepatitis B surface antigen (HBsAg) using the enzyme-linked immunosorbent assay (ELISA) technique. Malarial antigen and syphilis infection testing was done using a rapid diagnostic card test. The total number of sero-reactive cases and their distribution were noted. Results A total of 58,953 donors were screened and included during the study period. Each blood donor was identified by a donor registration number. The overall TTI seroprevalence in blood donors was 2.83% (n = 1670). The seroprevalence of TTIs in blood donors showed an increasing trend for HIV, HCV, and HBsAg in 2019–2021, whereas there was a decrease in reactivity status in the 2022 (back to pre-pandemic year). There was a significant increase in the coinfection rate from 0.1% to 0.25%. Conclusion The COVID-19 pandemic impact on the coinfection rate of TTI was significant. To curb these TTIs and coinfections, education and public awareness are the key factors. India is a developing country, so transfusion medicine specialists need to work day and night to practice safe blood services.

Introduction A shift in policy has occurred with the introduction of molecular diagnostic tools for the upfront diagnosis of all cases of tuberculosis, including drug-resistant tuberculosis. The impact of this shift in policy on severity of disease was studied, and comparisons were drawn between the year 2015 and 2020. Study Type and Design This was an observational study conducted between 2020 and 2021. Seventy patients of MDR-TB with or without additional drug resistance, hospitalized in the year 2015 and 2020, were studied. Results The study reveals a substantial reduction in the median time from the onset of symptoms to diagnosis between 2015 and 2020. Specifically, the median duration decreased from 12 weeks in 2015 to 8 weeks in 2020. Moreover, we found that in 2015, all cases under study had a history of tuberculosis in comparison to 2020. Additionally, there was a higher incidence of anemia in 2015 compared to 2020. In the radiological examination, it was observed that in 2015, a higher frequency of cases exhibited cavitations, bronchiectasis, and fibrosis on chest X-rays compared to the findings in 2020. The mean cavity size in 2015 measured 6.73 cm, while in 2020, it averaged 4.06 cm. Additionally, we noticed a higher occurrence of significantly advanced cases in 2015 in contrast to 2020. Conclusion The implementation of the new policy of upfront DST was noted to decrease the time required for diagnosis and bacterial load as ascertained from degree of sputum smear positivity, radiological lesions, and severity of anemia.

Background One of the most serious public health issues in India is reproductive tract infections (RTIs), which have a profound effect on the lives of men and women, as well as their families and communities. Objectives To describe the sociodemographic traits, assess women’s RTI knowledge, and determine which behavioral risk factors are involved in female RTI. Methods Using a 30-cluster sampling approach, a cross-sectional study design was employed to examine RTIs in 30 villages located in the Haldwani block of the district Nainital. Results It was shown that just 7.6% of women knew enough about RTIs. Among the 450 women surveyed, the percentage of RTI cases was significantly lower for those who used the barrier method during menstruation (7.7%), had more than three children (25.7%), had a trained person deliver their baby in a hospital (24.1%), used condoms as a family planning method (5.6%), used sanitary pads during menstruation (25.6%), and maintained good personal hygiene (22.4%). Conclusion The study revealed that the participants’ understanding of RTI was lacking.

Background and Aim: Patient movement during magnetic resonance imaging (MRI) is the most frequent cause of artifacts and poor scan quality. Children cannot lie still. Thus, anesthesia is required to keep the child calm and immobile. This randomized double-blinded clinical trial compares the clinical effects of the addition of dexmedetomidine as premedication with ketofol on the quality of sedation. We hypothesized that the addition of dexmedetomidine would improve the quality of sedation. Methods: A total of 132 children aged 6 months to 10 years were randomized into groups DK (dexmedetomidine–ketofol) and K (ketofol). DK received an intravenous bolus of dexmedetomidine (0.5 mcg/kg) as premedication 10 minutes prior. Both the groups were induced with ketofol (0.5 mg/kg), and sedation was maintained with propfol infusion (100 mcg/kg/min). The primary objective was the quality of sedation as assessed by the University of Michigan Sedation Scale. Image quality, requirement of rescue propofol dose, recovery, and adverse events were also studied. Data are given as median [interquartile range (IQR)] or frequency. Results: All 132 children completed MRI scans. The DK group showed significantly better quality of sedation, 71% versus 47% of children, a median difference of 1 (-0.569 to -0.0969), P < .005, a better quality of scan, a reduced number of additional doses of propofol, and a decreased total dose of propofol. Hemodynamic parameters and recovery times for the two groups were similar. There were no significant side effects in both groups. Conclusion: The quality of sedation and the quality of the MRI scan are greatly improved by administering dexmedetomidine (0.5 mcg/kg) 10 minutes before to induction. Additionally, this technique decreases the need of propofol and gives better hemodynamic stability without delaying the recovery time.

Background The presence of dental caries may have a negative impact on well-being of children and their parents, causing pain and discomfort, leading to poor oral health-related quality of life (OHRQoL) in them. The objective of this study was to evaluate the impact of different levels of caries experience on the OHRQoL of pre-school children and their families in Odisha, assessed using Odia version of Early Childhood Oral Health Impact Scale (Od-ECOHIS). Methodology A cross-sectional survey was carried out on a sample of 369 pre-school children using Od-ECOHIS. Dental caries was recorded using International Caries Detection and Assessment System (ICDAS) index. Result The total score of ECOHIS ranged from 0 to 42 with a mean of 2.4 (±5.3) and a median score of 0. The items bearing high mean scores in the child impact section were related to “pain”, “difficulty in drinking hot or cold beverages”, “difficulty in eating some food”, and “irritable or frustrated”. Though half of children (50.4%) had cavitated carious lesions, the prevalence of negative impact was 30.6%. Compared to the child being caries-free, the expected count of total Od-ECOHIS score increased by 1.54 times if the child had white spot lesion and 1.94 times if the child had a cavitated lesion. Conclusion Presence of dental carious lesions has a significant impact on OHRQoL of pre-school children and their families measured by using Od-ECOHIS even after adjusting for SES and caregivers’ perception of their children’s oral health.

Background: India is home to 14 million survivors of human trafficking, with most being trafficked for sexual exploitation. Trafficking constitutes crimes that violate the rights of survivors, and despite its psychological consequences, there is little evidence-based guidance to meet the needs of these individuals. Aim: The current study aimed to examine the outcome of narrative exposure therapy (KIDNET) among adolescent survivors of sex trafficking in improving trauma-related symptoms, along with psychological distress, dissociation, depression, and anxiety. Methods: A pre-post study design was used with 20 adolescent female survivors of trafficking aged 13 to 17 years, with ten participants in the KIDNET group and ten participants in a waitlist control group. Trafficking Victim Identification Tool and Standard Progressive Matrices were administered as screening measures. The Clinician-Administered PTSD Scale for Children and Adolescents, Kessler’s Psychological Distress Scale, Shutdown Dissociation Scale, Patient Health Questionnaire, and Beck Anxiety Inventory were administered as outcome measures. Fourteen sessions of KIDNET were conducted for the clinical group, while sessions were conducted for the control group post delivery of intervention for the clinical group. Results: Findings suggest an overall improvement in the severity of trauma-related symptoms in the KIDNET group, with significant improvement in psychological distress, depression, and anxiety. Conclusion: Results indicate that KIDNET may be a promising and acceptable treatment for adolescent survivors of sex trafficking, and this intervention module may be safely delivered in further randomised controlled trials to ensure that the holistic needs of this vulnerable group are appropriately addressed.

Background: The delivery of dental treatment might be negatively impacted by a child’s unwillingness to cooperate. Judging a behavior-guiding strategy that successfully lowers fear and anxiety during dental treatment, results in a change in the child’s attitude toward the treatment, and is also accepted by the parents is necessary given the variety of behavior management strategies that are available. While conventional techniques of behavior modification have been effective, parents’ and dental professionals’ attitudes regarding them are evolving. New nonaversive approaches that are acceptable to parents are thus required. One such approach that may find favor with parents and dental professionals is music therapy. Methods: Pertinent medical databases such as PubMed, Cochrane, Embase, and Google Scholar were searched from January 1, 2001, to April 20, 2023, for randomized controlled trials and clinical studies that used music and no music (control) in children undergoing dental treatment. Primary outcomes included a decrease in pulse rate as measured through pulse oximeter in children undergoing dental treatment. Results: Through search strategy and screening through titles and abstracts, four randomized clinical trials (Grading of Recommendations, Assessment, Development, and Evaluation [GRADE] high certainty) and eight nonrandomized studies (GRADE high certainty) were included and the pooled results bared that the music pulse rate was significantly reduced in children who were made to listen to the music than those who did not listen to music during dental treatment. Conclusion: It was established that music distraction was a better behavior management technique as compared to no music group and instrumental music was better when compared to the nursery rhyme for anxiety reduction in children.

Abstract Hydroforming is a modern metal-forming process prominently used in the shipbuilding, aerospace, and automotive industries for forming lightweight, complex-shaped geometries due to their inherent process benefits. But this process faces challenges such as limited material selection, high tooling costs, and complex process control for obtaining a defect-free part with uniform thickness. Researchers are constantly innovating and advancing hydroforming technologies to overcome these limitations. This work reviews emerging tube and sheet hydroforming technologies, considering parametric effects and design considerations, particularly for micro-domain applications. Further, a wider acceptance of the hydroforming process in different industries is explored by discussing the studies that tried to improve the efficiency and quality of the hydroforming process. This study observed that better formability could be achieved in hydroforming with appropriate intermediate heat treatment, proper lubrication, the correct design of loading paths, and temperature distribution. In tube hydroforming, wrinkles, necking, and cracking observed to be largely reduced by properly selecting the internal pressure and feeding. The precise protrusion height and uniform thickness at different joint cross-sections in tube forming are found to be significantly influenced by the strain-hardening exponent, loading path, and friction coefficient. Electrohydraulic forming is found being increasingly used due to its higher productivity and lower product cost. However, further research is required to achieve complex sheet geometries with sharp corners. This research emphasizes that advanced research, artificial intelligence integration, and the exploration of alternative materials can improve the performance of the hydroforming process.

The emergence of plant pathogens is often associated with waves of unique evolutionary and epidemiological events. Xanthomonas hortorum pv. gardneri is one of the major pathogens causing bacterial spot disease of tomatoes. After its first report in the 1950s, there were no formal reports on this pathogen until the 1990s, despite active global research on the pathogens that cause tomato and pepper bacterial spot disease. Given the recently documented global distribution of X. hortorum pv. gardneri, our objective was to examine genomic diversification associated with its emergence. We sequenced the genomes of X. hortorum pv. gardneri strains collected in eight countries to examine global population structure and pathways of emergence using phylodynamic analysis. We found that strains isolated post-1990 group by region of collection and show minimal impact of recombination on genetic variation. A period of rapid geographic expansion in X. hortorum pv. gardneri is associated with acquisition of a large plasmid conferring copper tolerance by horizontal transfer and coincides with the burgeoning hybrid tomato seed industry through the 1980s. The ancestry of X. hortorum pv. gardneri is consistent with introduction to hybrid tomato seed production and dissemination during the rapid increase in trade of hybrid seeds. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Abstract The increasing integration of new technologies is driving a fundamental revolution in the healthcare sector. Developments in artificial intelligence (AI), machine learning, and big data analytics have completely transformed the diagnosis, treatment, and care of patients. AI‐powered solutions are enhancing the efficiency and accuracy of healthcare delivery by demonstrating exceptional skills in personalized medicine, early disease detection, and predictive analytics. Furthermore, telemedicine and remote patient monitoring systems have overcome geographical constraints, offering easy and accessible healthcare services, particularly in underserved areas. Wearable technology, the Internet of Medical Things, and sensor technologies have empowered individuals to take an active role in tracking and managing their health. These devices facilitate real‐time data collection, enabling preventive and personalized care. Additionally, the development of 3D printing technology has revolutionized the medical field by enabling the production of customized prosthetics, implants, and anatomical models, significantly impacting surgical planning and treatment strategies. Accepting these advancements holds the potential to create a more patient‐centered, efficient healthcare system that emphasizes individualized care, preventive care, and better overall health outcomes. This review's novelty lies in exploring how these technologies are radically transforming the healthcare industry, paving the way for a more personalized and effective healthcare for all. It highlights the capacity of modern technology to revolutionize healthcare delivery by addressing long‐standing challenges and improving health outcomes. Although the approval and use of digital technology and advanced data analysis face scientific and regulatory obstacles, they have the potential for transforming translational research. as these technologies continue to evolve, they are poised to significantly alter the healthcare environment, offering a more sustainable, efficient, and accessible healthcare ecosystem for future generations. Innovation across multiple fronts will shape the future of advanced healthcare technology, revolutionizing the provision of healthcare, enhancing patient outcomes, and equipping both patients and healthcare professionals with the tools to make better decisions and receive personalized treatment. As these technologies continue to develop and become integrated into standard healthcare practices, the future of healthcare will probably be more accessible, effective, and efficient than ever before.

Abstract In this research article, the (1+1)- and (2+1)-dimensional Chiral nonlinear Schrödinger equations (CNLSEs) are studied, which play an important role in the development of quantum mechanics, particularly in the field of quantum Hall effect. Our primary goal is to obtain the analytical solutions utilizing novel methodology, particularly the modified extended tanh-function technique. We concentrate on the search to solitary wave solutions inside the (1+1)- and (2+1)-dimensional CNLSEs, which are relevant in domains such as optics, electro-magnetic wave propagation, plasma physics, optics and quantum mechanics. Our objective is to increase knowledge of this equation and give insight into the behavior of solitary waves by employing a novel mathematical technique. This will be accomplished by displaying our findings in 2D and 3D graphics. We believe that our results would pave a way for future research generating optical memories based on the nonlinear solitons.

Abstract Multiomic profiling of single cells by sequencing is a powerful technique for investigating cellular diversity. Existing droplet-based microfluidic methods produce many cell-free droplets, underutilizing bead barcodes and reagents. Combinatorial indexing on microplates is more efficient for barcoding but labor-intensive. Here we present Overloading And unpacKing (OAK), which uses a droplet-based barcoding system for initial compartmentalization followed by a second aliquoting round to achieve combinatorial indexing. We demonstrate OAK’s versatility with single-cell RNA sequencing as well as paired single-nucleus RNA sequencing and accessible chromatin profiling. We further showcase OAK’s performance on complex samples, including differentiated bronchial epithelial cells and primary retinal tissue. Finally, we examine transcriptomic responses of over 400,000 melanoma cells to a RAF inhibitor, belvarafenib, discovering a rare resistant cell population (0.12%). OAK’s ultra-high throughput, broad compatibility, high sensitivity, and simplified procedures make it a powerful tool for large-scale molecular analysis, even for rare cells.

Abstract Perceptual segregation of complex sounds such as speech and music simultaneously emanating from multiple sources is a remarkable ability that is common in humans and other animals alike. Unlike animal physiological experiments with simplified sounds or human investigations with spatially broad imaging techniques, this study combines insights from animal single-unit recordings with segregation of speech-like sound mixtures. Ferrets are trained to attend to a female voice and detect a target word, both in presence and absence of a concurrent equally salient male voice. Recordings are made in primary and secondary auditory cortical fields, and in frontal cortex. During task performance, representation of the female words becomes enhanced relative to the male in all, but especially in higher cortical regions. Analysis of the temporal and spectral response characteristics during task performance reveals how speech segregation gradually emerges in the auditory cortex. A computational model evaluated on the same voice mixtures replicates and extends these results to different attentional targets (attention to female or male voices). These findings underscore the role of the principle of temporal coherence whereby attention to a target voice binds together all neural responses coherently modulated with the target, thus ultimately forming and extracting a common auditory stream.

Abstract Background Social connection is a basic human need and is essential to quality of life. It is associated with better mental and physical health outcomes for long-term care (LTC) home residents and is a key aspect of quality of care and person-centred care. There are considerations for LTC homes that may present obstacles to and opportunities for social connection. It is therefore important to understand what restricts or enables good social connection in LTC homes, to guide better quality care and future interventions in this population. This qualitative study aims to identify barriers and facilitators to social connection for LTC residents. Methods We used thematic analysis to describe themes derived from individual and group qualitative interviews from 67 participants (18 residents, 17 staff members and clinicians, 32 family members and friends) recruited from LTC homes in the United Kingdom and Canada. Results Themes were grouped into four categories: (1) becoming familiar with life in the LTC home to support social connection; (2) physical and virtual access beyond the LTC home as strategies to maintain contact; (3) getting to know residents to deepen relationships; (4) person-centred approaches to build social connection. ‘Becoming familiar with life in the LTC home to support social connection’ described the benefits of counteracting the institutionalized feel of LTC homes, enabling LTC residents to spend time in meaningful ways, and increasing freedom of mobility around the home. ‘Physical and virtual access beyond the LTC home as strategies to maintain contact’ related to the benefits of outings, providing support with technology, and involving family and friends in LTC home life. ‘Getting to know residents to deepen relationships’ related to the benefits of using routine care and interactions as opportunities for social contact, using family and friend knowledge as a resource, and fostering resident relationships. ‘Person-centred approaches to build social connection’ included considering physical, mental, cognitive, and sensory impairments, accounting for adjustment and sociability, using communal spaces well, and prioritizing psychosocial needs. Conclusions This study identifies barriers and facilitators to social connection for LTC residents which can be addressed in care policies, staff selection and training, and can inform policies and interventions to build and maintain social connection in LTC homes. Clinical trial number clinicaltrials.gov ID NCT05315960.

Abstract Aboveground biomass density (AGBD) estimates from Earth Observation (EO) can be presented with the consistency standards mandated by United Nations Framework Convention on Climate Change (UNFCCC). This article delivers AGBD estimates, in the format of Intergovernmental Panel on Climate Change (IPCC) Tier 1 values for natural forests, sourced from National Aeronautics and Space Administration’s (NASA’s) Global Ecosystem Dynamics Investigation (GEDI) and Ice, Cloud and land Elevation Satellite (ICESat-2), and European Space Agency’s (ESA’s) Climate Change Initiative (CCI). It also provides the underlying classification used by the IPCC as geospatial layers, delineating global forests by ecozones, continents and status (primary, young (≤20 years) and old secondary (>20 years)). The approaches leverage complementary strengths of various EO-derived datasets that are compiled in an open-science framework through the Multi-mission Algorithm and Analysis Platform (MAAP). This transparency and flexibility enables the adoption of any new incoming datasets in the framework in the future. The EO-based AGBD estimates are expected to be an independent contribution to the IPCC Emission Factors Database in support of UNFCCC processes, and the forest classification expected to support the generation of other policy-relevant datasets while reflecting ongoing shifts in global forests with climate change.

Abstract Bacterial biofilms represent a prominent biological barrier against physical and chemical attacks. Disturbing the anaerobic microenvironment within biofilms by co-delivery of oxygen appears as a promising strategy to enhance the activity of an antibiotic. Here, we report the effect of oxygen-producing calcium peroxide nanoparticles (CaO2 NP) in combination with tobramycin sulfate (Tob). On Pseudomonas aeruginosa PAO1 biofilms in vitro, the additive effect of CaO2 NP towards Tob activity enhanced biofilm eradication by 2 log compared to Tob alone. For natural biofilms grown in the oral cavity of human volunteers in situ, treatment by CaO2 NP alone slightly increased the fraction of dead bacteria from 44% in various controls, including Tob alone, to 57%. However, the combination of CaO2 NP with Tob further increased the fraction of dead bacteria to 69%. These data confirm the intrinsic antimicrobial and antibiotic-potentiating effect of CaO2 NP also in a clinically relevant setting.

Abstract Background Type 2 diabetes (T2D) is one of the most prevalent chronic diseases worldwide and a leading cause of cardiorenal disease and mortality. Only one-third of individuals with T2D receive care as recommended by the American Diabetes Association’s clinical practice guidelines. Effective strategies are needed to accelerate the implementation of guideline concordant T2D care. Methods The Michigan Collaborative for Type 2 Diabetes (MCT2D) is a statewide population health collaborative quality initiative (CQI) developed to improve the care of all people with T2D in Michigan. MCT2D has developed a learning health system with physician organizations and their constituent practices to support quality improvement initiatives focused on (1) improving use of guideline-directed pharmacotherapy to improve cardiorenal outcomes, (2) increasing evidence-based use of continuous glucose monitoring, and (3) supporting use of lower carbohydrate eating patterns. Results Between 2021 and 2022, MCT2D recruited 28 of the 40 Michigan-based physician organizations participating in Blue Cross’ Physician Group Incentive Program with 336 constituent practices and 1357 physicians in primary care (304), endocrinology (21) and nephrology (11). In January 2022, baseline data included a sample of 96,140 unique individuals with T2D. The baseline HbA1c was ≤ 7.0% for 66.3% of patients (n = 32,787), while 14.9% of patients had a most recent HbA1c ≥ 8.0% (n = 7,393). The most recent body mass index (BMI) was ≥ 30.0 for 64.8% of patients (n = 38,516). Discussion MCT2D has organized a statewide collaborative to recruit and engage a diverse and large set of physician organizations and their constituent practices. This is a promising opportunity to accelerate adoption of guideline-concordant care for people with T2D and may be a model for other state or regional collaboratives. Future directions include specific evidence-based interventions targeted at reducing diabetes-linked comorbidities and associated healthcare costs as well as strategies focused on T2D prevention among at-risk populations.

Abstract In recent years, language practitioners have paid increasing attention to artificial intelligence (AI)’s role in language programs. This study investigated the impact of AI-assisted language assessment on L2 learners’ foreign language anxiety (FLA), attitudes, motivation, and writing skills. The study adopted a sequential exploratory mixed-methods design. Divided between an experimental group (receiving AI-assisted assessment) and a control group (receiving paper-format assessment), the participants were 70 intermediate English learners from two intact university classes in Bangladesh. The TOEFL iBT writing section measured writing skills, while the study also investigated perceptions and experiences of FLA, attitudes, and motivation using narrative frames. Thematic analysis of the narrative data showed that AI-assisted assessment greatly raised learners’ motivation, improved attitudes about language acquisition, and lowered FLA. According to quantitative analysis, the pretest writing abilities across groups showed no appreciable variation. Even though the difference was not statistically significant on the posttest, the experimental group exceeded the control group. The results of this study imply that AI-assisted assessments can generate a helpful learning environment, lower anxiety, improve attitudes, and increase motivation, thereby delivering useful information. Future studies should investigate long-term consequences, and further improvements to AI tools should optimize educational advantages—attitudes, motivation, and writing skills.

Abstract Lignocellulosic biomass (LB) represents a sustainable and abundant feedstock with diverse applications, particularly in microbial fermentation processes for the production of biofuels and biochemical. This biomass serves as a promising alternative to conventional fossil fuels. Extensive research on the decomposition of wood has elucidated various microbial enzymatic and non-enzymatic mechanisms that facilitate the utilization of this resource. This review systematically examines the chemical and structural factors contributing to biomass recalcitrance, emphasizing recent advancements in analytical tools for their assessment. Furthermore, we explore innovative predictive models for hydrolysis, which are crucial for optimizing the conversion of LB into biofuels and bio-based chemicals. A comprehensive cost analysis of hydrolysis processes is also presented, offering insights into the economic feasibility of these conversion methods. The findings underscore the potential of lignocellulosic biomass as a key player in sustainable energy and material production.

Abstract Background Medical education is tasked with shaping how medical students and physicians think, feel and act as professionals, or their Professional Identity Formation (PIF). This process has traditionally rested upon imparting knowledge; integrating sociocultural, professional and organizational expectations and codes of conduct; inculcating program and practice beliefs, values and principles (belief systems); and imbuing shared identities – quintessential elements that, together, comprise the socialization process. Key to supporting this socialization process is reflective practice. However, regnant approaches to mobilizing reflective cycles are faced with resource, personnel and time constraints, hindering efforts to nurture PIF. Group non-written reflections (GNWR) – broadly defined as facilitator-led discussions of shared reflective experiences within groups of learners – may prove to be an effective compromise. To address diverse approaches and a lack of effective understanding, we propose a systematic scoping review (SSR) to map the current use of GNWR in medical training and its role in shaping PIF. Methods Guided by the Systematic Evidence-Based Approach (SEBA)’s constructivist ontological and relativist epistemological position, this SSR in SEBA searched for articles on GNWR published in PubMed, Embase, Psychinfo, CINAHL, ERIC, ASSIA, SCOPUS, Google Scholar, Open Grey, GreyLit and ProQuest databases. The data found was concurrently analyzed using thematic and direct content analysis. Complementary themes and categories identified were combined, creating the domains that framed the discussion. Results Of the 8560 abstracts and 336 full-text articles reviewed, 98 articles were included. The four domains identified were: (1) Indications of use and their value; (2) Structure and how they can be used; (3) Models of reflective practice in GNWR; and (4) Features of communities of practice and the socialisation process. Conclusion This SSR in SEBA concludes that GNWR does impact PIF when effectively structured and supported. The Krishna-Pisupati Model for PIF platforms a model that explains GNWR’s effects of PIF and advances fourteen recommendations to maximize GNWR use.

Abstract Background Nutrient content and degree of processing are complementary but distinct concepts, and a growing body of evidence shows that ultra-processed foods (UPFs) can have detrimental health effects independently from nutrient content. 10 + countries currently mandate front-of-package labels (FOPL) to inform consumers when products are high in added sugars, saturated fat, and/or sodium. Public health advocates have been calling for the addition of ultra-processed warning labels to these FOPLs, but the extent to which consumers would understand and be influenced by such labels remains unknown. We examined whether the addition of ultra-processed warning labels to existing nutrient warning labels could influence consumers’ product perceptions and purchase intentions. Methods In 2023, a sample of adults in Brazil (n = 1,004) answered an open-ended question about the meaning of the term “ultra-processed,” followed by an online experiment where they saw four ultra-processed products carrying warning labels. Participants were randomly assigned to view either only nutrient warning labels or nutrient plus ultra-processed warning labels. Participants then answered questions about their intentions to purchase the products, product perceptions, and perceived label effectiveness. Results Most participants (69%) exhibited a moderate understanding of the term “ultra-processed” prior to the experiment. The addition of an ultra-processed warning label led to a higher share of participants who correctly identified the products as UPFs compared to nutrient warning labels alone (Cohen’s d = 0.16, p = 0.02). However, the addition of the ultra-processed warning label did not significantly influence purchase intentions, product healthfulness perceptions, or perceived label effectiveness compared to nutrient warning labels alone (all p > 0.05). In exploratory analyses, demographic characteristics and prior understanding of the concept of UPF did not moderate the effect of ultra-processed warning labels. Conclusions Ultra-processed warning labels may help consumers better identify UPFs, although they do not seem to influence behavioral intentions and product perceptions beyond the influence already exerted by nutrient warning labels. Future research should examine how ultra-processed warning labels would work for products that do and do not require nutrient warnings, as well as examine the benefits of labeling approaches that signal the health effects of UPFs. Trial registration ClinicalTrials.gov, NCT05842460. Prospectively registered March 15th, 2023.

Abstract Lemierre’s syndrome, a forgotten clinical entity, is characterised by septic thrombophlebitis of the internal jugular vein due to oropharyngeal infection. In the past, it was mainly associated with Fusobacterium necrophorum infections. We present a unique case report of Lemierre’s syndrome due to Klebsiella pneumoniae. Patient reported with progressive swelling involving right side of the neck that eventually increased in size. After confirming the diagnosis through CECT, patient was managed through combined medical and surgical therapy in the form of incision and drainage, targeted antibiotic therapy, glycaemic control and anticoagulation therapy. This case report highlights the association of poor glycaemic control in the pathogenesis and the isolation of Klebsiella pneumoniae as the rare organism of Lemierre’s syndrome.

Introduction: Epilepsy is a disorder characterised by two or more recurrent seizures that are unprovoked by any immediately identifiable cause. Additionally, it can lead to psychological issues, including anxiety and depression, as well as societal problems such as increased social stigma and withdrawal. Physical exercise regimens incorporated into the treatment plan can benefit epilepsy patients; however, these are often not included by medical experts or epilepsy patients themselves due to the stigma associated with the condition, apprehension that exercise might trigger seizures, or a lack of knowledge about the benefits of physical activity. Aim: To evaluate the effect of physical exercise on cardiovascular endurance and fitness levels in adults with and without epilepsy. Materials and Methods: A quasi-experimental study consisting of a pre-and post-design with a control group was conducted over a duration of six months. For both groups, participants were given exercise interventions three times a week for six months (from March 2022 to August 2022), with each session lasting 60 minutes. The outcome measures evaluated included the Shuttle Walk Test (SWT) and maximal oxygen consumption (VO2 max). For normally distributed data, an unpaired t-test was used to compare data between groups, while repeated measures Analysis of Variance (ANOVA) was used to compare data within the same group. A significance level of p-value ≤0.05 was considered. Results: The mean SWT scores in the control group at baseline, at the end of the 1st month, the 3rd month, and the 6th month were 6.62±1.32 m, 6.94±3.62 m, 7.46±2.69 m, and 8.36±3.16 m, respectively. In the experimental group, the mean SWT scores were 5.96±0.02 m, 6.68±2.24 m, 7.02±1.41 m, and 7.90±2.08 m, respectively. The mean VO2 max values in the experimental group at baseline, at the end of the 1st month, the 3rd month, and the 6th month were 29.76±4.29 m, 30.24±4.01 m, 33.40±2.17 m, and 35.45±3.68 m, respectively. In the control group, the mean VO2 max values were 29.59±5.68 m, 30.09±5.60 m, 31.22±3.48 m, and 33.88±4.26 m, respectively. Conclusion: The study concluded that the physical exercise intervention conducted for adults with and without epilepsy improved cardiovascular fitness and aerobic endurance. Therefore, patients with epilepsy should be encouraged to participate in some form of physical activity.

Abstract Background Respiratory Syncytial Virus (RSV) is an important pathogen causing acute respiratory illnesses in adults. RSV infection can lead to severe outcomes, including hospitalizations and even death. Despite the increased recognition of the burden in older adults, immediate post-discharge care needs among adults hospitalized with RSV are not well characterized and have not been compared to other serious medical conditions (such as influenza, acute myocardial infarction (MI), and stroke) for which there have been long-standing disease prevention efforts. Objectives This study aims to describe the immediate post-discharge care needs among adults hospitalized with RSV in the United States and descriptively compare it to those hospitalized with influenza, acute MI, or stroke. Design Retrospective observational cohort study. Patients Adults aged ≥ 18 years, hospitalized with a primary diagnosis of RSV, influenza, acute MI, or stroke from January 01, 2016, to December 31, 2019, were identified from the Premier Healthcare Database using the International Classification of Diseases (ICD-10) codes. Main measures Immediate post-discharge care was categorized into three different levels of care based on the discharge dispositions. Descriptive analyses were performed. Key results In total, 3,629 RSV, 303,577 influenza, 388,682 acute MI, and 416,750 stroke hospitalizations were identified, the majority occurred among patients aged ≥ 65 years. Professional home care needs were the highest for RSV hospitalizations (19.1%), followed by influenza (17.7%), stroke (15.4%), and acute MI (9.8%). Additionally, institutional care needs immediately following discharge were similar for RSV, influenza, and acute MI hospitalizations (14.2%, 15.8%, and 14.1%, respectively). Conclusions Immediate post-discharge care needs among adults hospitalized with RSV, especially in older adults, can be considerable and comparable to influenza and acute MI discharges. With recently approved RSV vaccines, efforts to increase vaccination in older adults are needed to prevent RSV and associated healthcare consequences.

Abstract Increasing evidence suggests an essential function for autophagy in unconventional protein secretion (UPS). However, despite its relevance for the secretion of aggregate-prone proteins, the mechanisms of secretory autophagy in neurons have remained elusive. Here we show that the lower motoneuron disease-associated guanine exchange factor Plekhg5 drives the UPS of Sod1. Mechanistically, Sod1 is sequestered into autophagosomal carriers, which subsequently fuse with secretory lysosomal-related organelles (LROs). Exocytosis of LROs to release Sod1 into the extracellular milieu requires the activation of the small GTPase Rab26 by Plekhg5. Deletion of Plekhg5 in mice leads to the accumulation of Sod1 in LROs at swollen presynaptic sites. A reduced secretion of toxic ALS-linked SOD1G93A following deletion of Plekhg5 in SOD1G93A mice accelerated disease onset while prolonging survival due to an attenuated microglia activation. Using human iPSC-derived motoneurons we show that reduced levels of PLEKHG5 cause an impaired secretion of ALS-linked SOD1. Our findings highlight an unexpected pathophysiological mechanism that converges two motoneuron disease-associated proteins into a common pathway.

Understanding species richness and diversity patterns and their governing factors in less-to-unexplored regions across Himalaya provide invaluable insights into exploring drivers which shape as well as influence plant community structures. The present investigation explores plant species richness and diversity patterns across different growth forms and its association with environmental parameters along altitudinal gradient (3200 m-4800 m) in alpine regions of west Himalaya, India. A total of 265 plant taxa were documented from study area with higher proportion of herbs (212), followed by shrubs (44) and trees (9). Species richness, diversity, and density patterns were estimated for each growth form along altitude gradients using polynomial regression and an apparent monotonically decreasing trend (p

Background: In July 2023, an outbreak of viral conjunctivitis swept across India. A knowledge of clinical features and outcome of treatment helps in the management of future outbreaks. AIM: The aim of this study was to report the clinical presentation, treatment, and outcome in patients affected during the outbreak of viral conjunctivitis. Setting and Design: This was a multicentric retrospective case series. MATERIALS AND METHODS: This study reviewed the medical records of all consecutive patients diagnosed with viral conjunctivitis between July 1, 2023, and August 10, 2023, in five centers across India. Results: Of the 793 patients, 525 (66.2%) were male and 268 (33.8%) were female, with a mean age of 34.1 ± 19.0 years. The mean symptom duration was 2.6 ± 2.1 days with bilateral disease in 591 (74.5%) patients. The common ocular signs were conjunctival congestion (793, 100%), lid edema (479, 60.4%), follicles (607, 76.5%), and conjunctival hemorrhages (339, 42.7%). Artificial tears were used in 730 (92.1%) patients, antibiotic eye drops in 661 (83.4%) patients, and steroid eye drops in 428 (54.0%). The time to resolution was the least in patients who had been treated with only artificial tears (4.61 ± 2.24 days) and was maximum in patients who had been treated with topical steroids (6.5 ± 2.51 days), and the difference was significant (P < 0.001). Conclusion: The presence of conjunctival hemorrhages indicated acute hemorrhagic conjunctivitis. Although many patients were treated with topical antibiotics, it was unnecessary, as the shortest resolution time was found in patients who had been received supportive therapy with artificial tear eye drops and not topical antibiotics or steroids.

Background: A healthy lower back is essential for optimal spinal function and overall wellness. Magnetic resonance imaging (MRI) has become the gold standard in assessing lumbar spine disease. This article aims to evaluate the precision and efficacy of the lumbar offset distance (LOD) as a novel MRI parameter designed to determine the lumbar spine alignment. normally measured as we compared it to a new parameter based on length. MATERIALS AND METHODS: Supine sagittal magnetic resonance images of 101 patients who underwent lumbar spine MRI scans were analyzed. We focused on L1–L5 lumbar lordosis angle (LLA) and LOD to assess lumbar spine alignment. Diagnostic cutoff values for LOD measurements were determined, and their diagnostic accuracies were evaluated. Results: The normal LLA in our dataset was 23°–45°, and the normal LOD was 5–15 mm. Using linear regression, the range of 6–14 mm correlates to the LLA range of 20°–45°, which would define the standard lumbar offset as normal between 6 and 14 mm. Hence, lumbar hypolordosis was defined as

Background: Cognitive and autonomic dysfunction is increasingly being recognized as an important clinical dimension in major depressive disorder. AIM: The aim of this study is to evaluate the effect of a combined approach of yoga and diet intervention on cognitive and autonomic functions in individuals with major depressive disorders. Methods: This experimental observational study was conducted at RUHS College of Medical Sciences and Associated Hospitals, Jaipur, on the major depressive disorder population of either sex cognitive function (mini-mental score, Montreal cognitive protocol A and B, P300 latency and amplitude) and autonomic function parameters (frequency and time domain) were recorded at baseline and after three months of a combined approach of yoga and diet intervention. Results: This study compared cognitive and autonomic function parameters at baseline and after three months of yoga and diet intervention in a major depressive disorder population. Analysis revealed a significant decrease in body mass index (

Background and Purpose Cerebral venous thrombosis (CVT) is associated with a high degree of morbidity and mortality. Our objective is to elucidate characteristics, treatments, and outcomes of patients with cancer and CVT (CA-CVT). Methods The 2016–2019 National Inpatient Sample (NIS) database was queried for patients with a primary diagnosis of CVT. Patients with a currently active diagnosis of malignancy (CA-CVT) were then identified. Demographics and comorbidities were compared between CA-CVT and CVT patients. Subgroup analyses explored patients with hematopoietic cancer and non-hematopoietic cancers. Stroke severity and treatment were explored. Inpatient outcomes studied were discharge disposition, length of stay, and mortality. Results Between 2016 and 2019, 6,140 patients had a primary diagnosis code of CVT, and 370 (6.0%) patients had a coexisting malignancy. The most common malignancy was hematopoietic (n=195, 52.7%), followed by central nervous system (n=40, 10.8%), respiratory (n=40, 10.8%), and breast (n=40, 10.8%). These patients tended to be older than non-CA-CVT and were more likely to have coexisting comorbidities. CA-CVT patients had higher severity scores on the International Study of Cerebral Vein and Dural Sinus Thrombosis Risk Score (ISCVT-RS) and increased complications. In a propensity-score matched cohort, there were no differences in inpatient outcomes. Conclusion Malignancy occurs in 6% of patients presenting with CVT and should be considered a potential comorbidity in instances where clear causes of hypercoagulabilty have not been identified. Malignancy was linked to higher mortality rates. Nonetheless, after adjusting for the severity of CVT, the outcomes for inpatients with cancer-associated CVT were comparable to those without cancer, indicating that the increased mortality associated with malignancy is probably due to more severe CVT conditions.

Abstract Background Salt stress is a prominent abiotic stressor that imposes constraints on grain yield and quality across various crops, including wheat (Triticum aestivum). This study focused on assessing the genetic diversity of 20 wheat genotypes categorized as tolerant, moderately tolerant, and sensitive with three genotypes of unknown tolerance. To address salinity stress-related problems, different morpho-physiological, osmoprotectant, biochemical, yield, and grain quality-related parameters were analyzed under control (pH 8.0, EC 3.9) and saline-sodic (pH 9.4, EC 4.02) conditions in field. Results Findings revealed noteworthy variations among the genotypes in response to salinity stress. Greater accumulation of Na+ and lower K+ content were observed in response to salt stress in the sensitive varieties HD1941 and K9162. Proline, a stress indicator, exhibited significantly (p ≤ 0.05) greater accumulation in response to salinity stress, particularly in the tolerant cultivars KRL210 and KH65. Salt stress induced the most significant decrease (p ≤ 0.05) in spike length, thousand-grain weight, and hectolitre weight coupled with increased protein content in sensitive varieties, resulting in diminished yield. Conclusion Correlation analysis of parameters under salinity stress showed that SOD, proline, and K+ contents can be used as the most efficient screening criteria for salinity stress during early developmental stages. Principal component analysis revealed that DBW187, DBW303, and DBW222 varieties were tolerant to salinity stress and exhibited an effective antioxidant system against salinity. This study will facilitate salt-tolerant wheat breeding in terms of the identification of tolerant lines by screening for limited traits in a wide range of germplasms.

Abstract The ever-increasing demand for sustainable energy has drawn attention towards photovoltaic efficiency and reliability. In this context, the shading and associated hotpot degradation within PV modules has become an important area of research and development. The experimental approach of this paper aims to investigate single cell shading in high efficiency monocrystalline silicon PV PERC modules. Prior to the outdoor experiment, the PV module underwent experimental testing under STC to determine variation in electrical and thermal behaviour due to partial shading. The indoor experiments are performed using Sun-simulator and the I–V and P–V curves are analysed. Further, the outdoor experiments were performed under realistic conditions. In both cases, results showed that during 40–60% shading in single cell leads to critical shading scenario causing significant drop in power output in comparison with their unshaded conditions. The maximum power loss of 36.34% and 42% is recorded for indoor and outdoor experiments. The outdoor experiments recorded hotspot temperature of 85–90.1 °C under respective 40% and 60% critical shading scenarios. The efficiency recorded in the time interval of 11:00:00 and 11:30:00 was highest for the solar radiations between 940 and 990 W/m2. The maximum drop in efficiency is recorded from noon till 13:30:00 time of the day. Development of hotspot is directly related to the failure or malfunction of protecting system. Hence the importance of type of PV technology, amount of shading, and critical shading scenario is presented in the study. This study is important for researcher and manufacture to consider single cell shading in PV technology.

Abstract Background The coronavirus disease 2019 (COVID-19) pandemic induced psychological distress, which is linked to emotional eating and symptoms of addiction to ultra-processed foods (UPFs). Objective This study aimed to investigate whether symptoms of addiction to UPFs mediate the relationship between psychological stress due to the COVID-19 pandemic and emotional eating behaviour. Methods A cross-sectional online study was conducted from May to November 2021 among 368 undergraduate Brazilian students. The participants answered demographic questions and completed validated scales, including the Coronavirus Stress Measure, Modified Yale Food Addiction Scale 2.0 and Emotional Eating Questionnaire. Mediation analysis was employed to examine the hypothesised relationships. Results The results revealed a significant indirect effect, indicating that symptoms of food addiction mediated the association between perceived stress during the COVID-19 pandemic and emotional eating behaviour. Specifically, 61% of the influence of perceived stress on emotional eating during the pandemic was explained by symptoms of UPF addiction. Conclusion These findings suggest that addressing symptoms of UPF addiction could be pivotal in public health strategies aimed at promoting healthy eating habits among distressed undergraduate students in the post-COVID-19 era.