109 results on '"Lozano JC"'
Search Results
2. ESTUDIOS ALEATORIOS SOBRE EL EFECTO DEL SENIOR FITNESS TEST EN LA TERCERA EDAD
- Author
-
Gómez Figueroa, JA, primary, Castineyra Mendoza, S., additional, Rodríguez Cervantes,, VO, additional, Quintana Rivera, L., additional, Chacón Rodríguez, CM, additional, Rivera Girón, AJ, additional, Gasperin Rodríguez, EI, additional, Molina Arriola, JE, additional, and Flores Lozano, JC, additional
- Published
- 2019
- Full Text
- View/download PDF
3. PGD of β-thalassaemia and HLA haplotypes using one-step multiplex PCR
- Author
-
Peciña, A, primary, Lozano, MD, additional, García-Lozano, JC, additional, and Antiñolo, G, additional
- Published
- 2009
- Full Text
- View/download PDF
4. 7.001 One-step multiplex PCR for preimplantation genetic diagnosis of Huntington's disease
- Author
-
Peciña, A, primary, Lozano, MD, additional, Moya, C, additional, Garcí-Lozano, JC, additional, Carrillo-Vadillo, R, additional, Borrego, S, additional, and Antiñolo, G, additional
- Published
- 2008
- Full Text
- View/download PDF
5. 9.002 Vitrification of biopsied blastocysts using a closed method
- Author
-
Lozano, MD, primary, Peciña, A, additional, Moya, C, additional, Garcí-Lozano, JC, additional, Carrillo-Vadillo, R, additional, Borrego, S, additional, and Antiñolo, G, additional
- Published
- 2008
- Full Text
- View/download PDF
6. Screening for Resistance to Cassava Bacterial Blight
- Author
-
Lozano Jc and Laberry R
- Subjects
biology ,Resistance (ecology) ,Botany ,Manihot esculenta ,Euphorbiaceae ,Bacterial blight ,Plant Science ,Cultivar ,biology.organism_classification ,Agronomy and Crop Science ,Xanthomonas campestris ,Bacteria - Abstract
La resistance a Xanthomonas campestris pv. manihotis chez le manioc ou la maladie est endemique. Des essais en serre completent les resultats des observations au champ
- Published
- 1982
- Full Text
- View/download PDF
7. Safety and effectiveness of preoperative stenting compared to non-stenting in ureteroscopy for urinary stone disease: a meta-analysis of comparative studies.
- Author
-
Calvillo-Ramirez A, Angulo-Lozano JC, Del Rio-Martinez CJ, Esparza-Miranda LA, Perez Rodriguez Garcia G, Macías-Cruz HM, Neto-Vivas BP, and Gonzaga-Carlos N
- Subjects
- Humans, Treatment Outcome, Ureteral Calculi surgery, Preoperative Care methods, Urinary Calculi surgery, Kidney Calculi surgery, Ureteroscopy methods, Stents
- Abstract
Purpose: Ureteroscopy (URS) is considered one of the first-line surgical treatments for urinary stones < 2 cm. Preoperative stenting (PS) employment in URS for urolithiasis remains debated, with evidence differing in terms of outcomes and recommendations. We sought to evaluate the influence of PS on surgical outcomes compared to non-PS (NPS) in patients with renal and ureteral stones undergoing ureteroscopic lithotripsy., Methods: Databases were searched until December 2023 for randomized and non-randomized studies reporting perioperative outcomes for PS and NPS. Pooled data were analyzed through a Random-Effects model when Higgins I2% heterogeneity values were > 50%; otherwise, a Fixed-Effects model was employed. Results were reported as risk ratios (RR), or mean differences (MD) with 95% confidence intervals (CI). Statistical significance was set at p < 0.05., Results: The analysis included 23,668 patients from 25 included studies (5 non-randomized prospective and 20 retrospective studies). Higher stone-free rates (SFR) were observed in kidney and ureteral stones (RR 1.05; 95%CI 1.03-1.08; p ≤ 0.0001), especially if managed with flexible URS (RR 1.05; 95%CI 1.01-1.09) in the PS cohort. Additionally, lower rates of intraoperative (RR 0.70; 95%CI 0.49-0.99; p = 0.04) and postoperative complications (RR 0.82; 95%CI 0.70-0.95; p = 0.008) were seen with PS. Both groups had a comparable operative time, length of stay (LOS), ureteral access sheath (UAS) placement success, and SFR in semi-rigid URS., Conclusion: Higher SFR with PS were seen in kidney and ureteral stones, especially if f-URS was employed. Moreover, perioperative complications did not increase with PS. Future randomized studies, evaluating cost-effectiveness and quality of life are needed., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
- Full Text
- View/download PDF
8. Reply to Editorial Comment on "Comparative Outcomes of Day-Case Percutaneous Nephrolithotomy Versus Conventional Inpatient Surgery: A Systematic Review and Meta-Analysis".
- Author
-
Calvillo-Ramirez A and Angulo-Lozano JC
- Abstract
Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare.
- Published
- 2024
- Full Text
- View/download PDF
9. An INDEL genomic approach to explore population diversity of phytoplankton.
- Author
-
Devic M, Dennu L, Lozano JC, Mariac C, Vergé V, Schatt P, Bouget FY, and Sabot F
- Subjects
- Genetic Variation, Genomics methods, Genotype, Phylogeny, Phytoplankton genetics, Phytoplankton classification, INDEL Mutation
- Abstract
Background: Although metabarcoding and metagenomic approaches have generated large datasets on worldwide phytoplankton species diversity, the intraspecific genetic diversity underlying the genetic adaptation of marine phytoplankton to specific environmental niches remains largely unexplored. This is mainly due to the lack of biological resources and tools for monitoring the dynamics of this diversity in space and time., Results: To gain insight into population diversity, a novel method based on INDEL markers was developed on Bathycoccus prasinos (Mamiellophyceae), an abundant and cosmopolitan species with strong seasonal patterns. Long read sequencing was first used to characterize structural variants among the genomes of six B. prasinos strains sampled from geographically distinct regions in the world ocean. Markers derived from identified insertions/deletions were validated by PCR then used to genotype 55 B. prasinos strains isolated during the winter bloom 2018-2019 in the bay of Banyuls-sur-Mer (Mediterranean Sea, France). This led to their classification into eight multi-loci genotypes and the sequencing of strains representative of local diversity, further improving the available genetic diversity of B. prasinos. Finally, selected markers were directly tracked on environmental DNA sampled during 3 successive blooms from 2018 to 2021, showcasing a fast and cost-effective approach to follow local population dynamics., Conclusions: This method, which involves (i) pre-identifying the genetic diversity of B. prasinos in environmental samples by PCR, (ii) isolating cells from selected environmental samples and (iii) identifying genotypes representative of B. prasinos diversity for sequencing, can be used to comprehensively describe the diversity and population dynamics not only in B. prasinos but also potentially in other generalist phytoplankton species., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
10. Oncologic outcomes of pelvic organ-preserving radical cystectomy vs. Standard radical cystectomy: A systematic review and meta-analysis.
- Author
-
Hinojosa-Gonzalez DE, Saffati G, Wahlstedt E, Chaput M, Patel SR, Salgado-Garza G, Kronstedt S, Segall MR, Angulo-Lozano JC, Jones JA, Taylor JM, and Slawin JR
- Abstract
Background and Objective: Radical Cystectomy is indicated in muscle-invasive bladder cancer and select cases of nonmuscle invasive bladder cancer. Women often undergo additional reproductive organ removal, greatly impacting sexual function and quality of life. Pelvic organ-preserving radical cystectomy aims to mitigate these effects, but its oncologic outcomes are not well-defined. This presents a meta-analysis of available literature on oncological outcomes of pelvic organ-preserving radical cystectomy in women with muscle invasive disease., Methods: A systematic search across PubMed, Web of Science, Scopus, and Google Scholar was performed to identify studies comparing oncological outcomes between pelvic organ-preserving radical cystectomy and standard radical cystectomy in women with muscle-invasive bladder cancer or high-risk or recurrent nonmuscle invasive cancer. The search included English or Spanish studies, statistically comparing overall survival, cancer-specific survival, and recurrence-free survival. Statistical analysis used Review Manager, employing fixed or random-effects models based on heterogeneity., Key Findings and Limitations: Six retrospective studies met inclusion criteria, totaling 597 patients of which 303 received pelvic organ-preserving radical cystectomy and 294 received standard radical cystectomy. Overall Survival was not different between the 2 groups (HR 1.05 [0.77, 1.43]; P = 0.77). Cancer-Specific Survival also was found to be not different between the 2 groups (HR 1.27 [0.86, 1.87]; P = 0.22). Additionally, recurrence-free survival was not different between the 2 groups (HR 0.85 [0.41, 1.75]; P = 0.65. Four of the included studies exhibited a moderate risk of bias, with 1 study demonstrating low risk and the remaining study manifesting a serious risk of bias., Conclusion: The comparison showed no significant differences in overall survival, cancer-specific survival, or recurrence-free survival rates., Competing Interests: Declaration of competing interest There are no conflicts of interest to disclose., (Published by Elsevier Inc.)
- Published
- 2024
- Full Text
- View/download PDF
11. Parental Knowledge, Beliefs, Practices, and Barriers Related to Children's Bladder Health in the School Environment.
- Author
-
Venkatapuram P, Angulo-Lozano JC, Spinzi S, Brown-Johnson C, Phord-Toy A, and Kan KM
- Subjects
- Humans, Female, Child, Male, Adult, Child, Preschool, Lower Urinary Tract Symptoms, Parents psychology, Health Knowledge, Attitudes, Practice, Schools
- Abstract
Background: Pediatric lower urinary tract symptoms (LUTS) are a set of common childhood problems. Community-level interventions that target behavioral change among children with LUTS can improve symptoms outside of the clinic environment. Parents, navigating the home and school environments, are key in supporting healthy bladder behaviors. Thus, we asked parents about their perceptions and barriers related to pediatric bladder health., Methods: English-speaking parents (n = 30) of children ages 5-10 years with and without LUTS were interviewed. Transcripts were coded iteratively by two independent coders using deductive and inductive approaches that emphasized consensus coding and peer debriefing., Results: Ninety-three percent of participants were women, 50% were 30-39 years old, and 60% held a graduate degree. Parents identified school-, classroom-, and child-based barriers to bladder health. These included the bathroom environment, restrictive policies for bathroom use, and anxiety on how and when to use the bathroom., Conclusions: Addressing school-, classroom-, and child-based barriers is necessary to promote healthy bladder habits among children in the school environment and beyond. Recommended school-based interventions include bathroom use and sanitation policies that support students' voiding needs, teachers' professional development, and school readiness initiatives. Limitations include participation of English-speaking parents only., (© 2024 The Authors. Journal of School Health published by Wiley Periodicals LLC on behalf of American School Health Association.)
- Published
- 2024
- Full Text
- View/download PDF
12. Comparative Outcomes of Day-case Percutaneous Nephrolithotomy Versus Conventional Inpatient Surgery: A Systematic Review and Meta-analysis.
- Author
-
Calvillo-Ramirez A, Angulo-Lozano JC, Acevedo-Rodriguez JE, Vidal-Valderrama CE, and Antunez-Perez R
- Abstract
Objective: To conduct a systematic review and meta-analysis to evaluate the effectiveness and safety of PCNL performed as a day-case surgery in comparison to inpatient PCNL., Methods: Databases were searched up to January 27, 2024, for randomized and non-randomized studies comparing outcomes between day-case PCNL and inpatient PCNL. Pooled data were analyzed using a random-effects (RE) model when Higgins I2% heterogeneity values were >50%; otherwise, a fixed-effects model was employed. The results were reported as odds ratios (OR), mean difference (MD) or standardized MD (SMD) with 95% confidence intervals (CI). Statistical significance was set at P <.05., Results: One randomized controlled trial (RCT) and 14 observational studies totaling 1574 patients were included. Pooled results revealed that patients in the day-case PCNL group experienced reduced blood loss (SMD -0.71 95% CI: [-1.31, -0.12] P .02) and transfusion rates (OR 0.10 95% CI: [0.03, 0.39], P .0008), lower overall and minor complications (OR 0.56, 95% CI: [0.42, 0.76], P .0002, and OR 0.52, 95% CI: [0.37, 0-73], P .0002, respectively), shorter operative time (MD -11.46, 95% CI: [-17.41, -5-50], P <.00001), and reduced total costs (MD -1597.18, 95% CI [-2436.42, -757.93], P .0002). Major complications, stone-free rate (SFR), emergency department (ED) visits, and readmission rates were similar between the groups., Conclusion: Day-case PCNL is a feasible and safe alternative to inpatient PCNL surgery in carefully selected patients, without increasing the risk of complications or readmission rates, and is likely to reduce total costs., Competing Interests: Declaration of Competing Interest The authors have no conflict of interest to declare., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
13. Molecular characterisation of sickle cell disease and classification of major haplotypes associated with the β-globin cluster (HBB gene) by means of SNP marker sequencing in a group of samples from Bolívar, Colombia.
- Author
-
Díaz-Matallana M, Briceño I, Benavides-Benítez E, Bernal JE, and Martínez-Lozano JC
- Subjects
- Humans, Haplotypes, Colombia, beta-Globins genetics, Polymorphism, Single Nucleotide, Anemia, Sickle Cell epidemiology, Anemia, Sickle Cell genetics, Anemia, Sickle Cell diagnosis
- Abstract
Background: Colombia has a mestizo population and the prevalence of haemoglobin variants varies according to each region, but heterozygous carriers can be found in all of them., Aim: To characterise sickle cell disease (SCD) haematologically, biochemically, and molecularly, and detect classic haplotypes by DNA sequencing in a group of samples from Bolívar, Colombia., Subjects and Methods: Blood samples were collected after informed consent from volunteers from eight communities in the Bolívar department, plus samples from the Pacific region, Providencia Island, and Bogotá were included. Data were obtained from: (1) haematological analyses; (2) biochemical tests: dHPLC was used to determine haemoglobin (Hb); and (3) DNA sequencing data through five SNPs., Results: 101 samples were identified by rs334 through Sanger's Sequencing, structural haemoglobinopathies HbAS (34.65%), HbSS (2.97%) and HbAC (1.98%) were found. When contrasting the Hb identification results between SNP rs334 Vs. dHPLC/Isoelectric Focusing (IEF), a coincidence was found in 39/43 samples analysed, therefore, when comparing these techniques, a significant correlation was found (Pearson's correlation coefficient r = 0.998). 26 samples previously analysed by rs334 were classified into classical haplotypes CAR (50.0%), BEN (30.76%), CAM (7.69%), SEN (3.84%), and ATP-I (7.69%)., Conclusions: SCD characterisation and SNPs-based classification through Sanger's DNA sequencing have not been performed before in Colombia. The results of this work will make it possible to expand the data or records of carriers and those affected, which will benefit patients and their families.
- Published
- 2024
- Full Text
- View/download PDF
14. Intraoperative Magnetic Resonance Imaging (MRI)-Guided Resection of Glioblastoma: A Meta-Analysis of 1,847 Patients.
- Author
-
Pichardo-Rojas PS, Angulo-Lozano JC, Alvarez-Castro JA, Vázquez-Alva D, Osuna-Lau RA, Choque-Ayala LC, Tandon N, and Esquenazi Y
- Subjects
- Humans, Neurosurgical Procedures methods, Magnetic Resonance Imaging methods, Glioblastoma diagnostic imaging, Glioblastoma surgery, Glioblastoma pathology, Brain Neoplasms diagnostic imaging, Brain Neoplasms surgery, Brain Neoplasms pathology
- Published
- 2024
- Full Text
- View/download PDF
15. Natural radionuclides behaviour in drinking groundwaters from Castilla y León (Spain); radiological implications.
- Author
-
Borrego-Alonso D, Quintana-Arnés B, and Lozano JC
- Abstract
Since the coming into force of the European Council Directive 51/2013 EURATOM and its transposition into the Spanish legislation, the presence of radioactive substances in drinking waters must be kept under surveillance to ensure that the health protection requirements are met. Driven by this regulatory framework, in an attempt to know the starting point from which to design surveillance plans, the groundwaters intended for human consumption of Castilla y León (Spain) have been radiologically characterised by using both low-level γ-ray and α-particle spectrometry to determine the activity concentration of the natural radionuclides needed to account for the indicative dose estimation. This extensive research has comprised the radiological characterisation of more than 400 drinking water samples from one of the European Union's largest regions. Furthermore, the gross α and gross β activities have been analysed. Results showed a high geographical variability that can be related to the hydrogeological formations where the groundwaters come from. The uranium isotopes,
234 U and238 U, are the main radionuclides present in the analysed drinking waters reaching values up to 2000 mBq/L, in the southwestern and western of Castilla y León, where U-rich minerals are part of the host rock. High210 Pb and226,228 Ra occurrences are found in the low permeability igneous and metasedimentary hydrogeological formations of Salamanca province. From a public health protection point of view, 4.4% of the total drinking water samples from intakes exceeded the Indicative Dose parametric value of 0.1 mSv, which is a not negligible number of samples, being very likely related to granitic and metamorphosed host rock under specific local conditions. This fact highlights the need for research and consideration of special surveillance of the groundwaters from these areas., Competing Interests: Declaration of Competing Interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Characterisation of the Natural Radionuclide Budget in Drinking Waters from Aquifers of Castilla y León (Spain): Does it Pose Risks for Public Health?”, (Copyright © 2023. Published by Elsevier Ltd.)- Published
- 2023
- Full Text
- View/download PDF
16. Revisiting methods for the assessment of naturally-occurring radioactivity in drinking water.
- Author
-
Borrego-Alonso D, Quintana B, and Lozano JC
- Subjects
- Humans, Reproducibility of Results, Drinking Water analysis, Radioactivity, Radiation Monitoring methods, Water Pollutants, Radioactive analysis
- Abstract
A comprehensive radioactivity characterisation of Castilla y León (Spain) groundwater intakes intended for human consumption is addressed between 2017 and 2021. This study responds to the radiological requirements stated in the European Council Directive 51/2013/EURATOM concerning drinking water for public health. Given that the gross α- and gross β-activity determination is used as a screening strategy for water radioactivity monitoring, the reliability of the thin-source deposit method (ISO 10704, 2019) has been improved to achieve representative values of the total activity coming from α- and β-particle emitters in the samples. More than 400 groundwater samples have been considered for this purpose, analysing their
238,235,234 U,228,226 Ra,210 Pb, and210 Po activity concentrations, which are needed for the indicative dose assessment. These results, considered reference activity values, are compared with the gross α- and gross β-activities determined by the different procedure approaches. Ultimately, the results of the liquid scintillation counting method (ISO 11704, 2018) are also used to validate the optimised method., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
17. Disparities in Healthcare Access, Education, and Geographical Factors That Affect Surgical Outcomes in Penile Cancer.
- Author
-
Angulo-Lozano JC, Sánchez Musi LF, and Garcia Garcia J
- Abstract
Objectives: To establish the level of access to healthcare, education, social and geographical factors predisposing a negative surgical outcome and higher mortality rate in patients with penile cancer., Methods: This is a retrospective, longitudinal and analytical study. Ninety-three medical records of adult male patients diagnosed with penile cancer were reviewed. Fisher's exact test was performed to determine the association between the level of healthcare, social and geographical factors, and the outcomes for penile cancer., Results: Patients without primary care access had a higher chance of having lymphovascular invasion at the time of diagnosis (OR=37.5, P<0.0001), a higher mortality rate at 24 months after diagnosis (OR=19.2, P=0.005), a lack of high school diploma or equivalent (OR=3.8, P=0.049) and a higher likelihood of referral from a provincial hospital (OR=10.1, P<0.0001). Patients without a favorable surgical outcome (radical penectomy) were more likely to have been referred from a provincial hospital (OR=6.8, P<0.0001) and not have access to a primary care center (OR=149.5, P<0.0001), a tertiary care center (OR=20.7, P=0.003), and a high school diploma (OR=7.9, P=0.004). Conclusions: The lack of access to primary care is strongly associated with vascular invasion at diagnosis, lower educational level, a referral from provincial zones, and a higher mortality rate at 24 months. Patients who did not have access to primary and tertiary care, a high school diploma, and were referred from the province were more likely to have a radical penectomy., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Angulo-Lozano et al.)
- Published
- 2022
- Full Text
- View/download PDF
18. Association Between Testicular Microlithiasis and Histological Subtype in Testicular Cancer.
- Author
-
Gonzaga-Carlos N, Virgen-Gutierrez F, Angulo-Lozano JC, Virgen-Rivera MF, Maldonado-Avila M, and Jaspersen Gastelum J
- Abstract
Background There is a clear association between testicular cancer and microlithiasis when there are predisposing risk factors such as the history of germ cell tumors in first-degree relatives, testicular atrophy, Klinefelter's syndrome, and cryptorchidism. This study aimed to establish an association between microlithiasis and the histological subtype of testicular cancer by analyzing data on Hispanic population. Methods A retrospective cohort, longitudinal, comparative, and analytic study was conducted on patients with a confirmed diagnosis of primary testicular cancer. The testicular ultrasounds were checked before any surgical treatment to find microlithiasis. We performed a binary logistic regression to establish an association between microlithiasis and the type of testicular cancer. Results A total of 130 clinical files were analyzed. Binary logistic regression showed no association between testicular microlithiasis and the subtype of testicular cancer (p = 0.438, 95% CI: 0.80-1.64). The result of the Pearson chi-square test showed no association (p = 0.184). We also analyzed the association between age and microlithiasis using the one-way ANOVA test (p = 0.82) and the association between age and the dichotomic subtype of testicular cancer (seminomatous and non-seminomatous) using the ANOVA one-way test, which showed no significant association in age and testicular cancer subtype (p = 0.178). Conclusions There was no association between testicular microlithiasis and the histological subtype of testicular cancer in our study. As mentioned before, we recommend conducting a more extensive study to provide further scientific evidence to establish a reliable association between microlithiasis and the subtype of testicular cancer since there is a discrepancy in the results of our study with the information previously reported. We encourage the study of characterization of risk factors among ethnic groups as this field has not been explored yet., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Gonzaga-Carlos et al.)
- Published
- 2022
- Full Text
- View/download PDF
19. A New Endoscopic Approach to Remove a Retained Stingray Barb.
- Author
-
Palatchi Oldak J and Angulo-Lozano JC
- Abstract
Stingray injuries usually happen when a person steps into one, resulting in the attack of the fish introducing a sting into the feet or leg in a defensive manner, causing significant pain and inflammation due to the venom. Retained stingray barb occurs in a low percentage of these accidents and may be difficult to diagnose, for this reason, it is always recommended to use imaging studies. In this case report, we present a 42-year-old man who presented to the orthopedic consult one week after a stingray injury with the diagnosis of retained stingray barb. The aim of this case report is to present a new surgical approach for stingray barb removal with a minimally invasive technique using arthroscopic equipment using the stingray barb entry site as the port to introduce the endoscopic tools., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Palatchi Oldak et al.)
- Published
- 2022
- Full Text
- View/download PDF
20. Immunotherapy With Bacillus Calmette-Guerin (BCG) in a 16-Year-Old With Urothelial Bladder Carcinoma.
- Author
-
Martinez-Thomas JM, Galicia-Belaunzaran LF, Merayo-Chalico CE, Palatchi J, and Angulo-Lozano JC
- Abstract
Urothelial bladder cancer (UBC) is an exceptionally rare condition in adolescents between 15 and 19 years of age. Typically, adolescents and pediatric patients with UBC are more likely to have a favorable histological report. The aim of the paper is to report our experience in the management of a 16-year-old patient with UBC with no risk factors that came to the office because of a history of painless gross hematuria., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Martinez-Thomas et al.)
- Published
- 2022
- Full Text
- View/download PDF
21. Wunderlich Syndrome Associated With Angiomyolipomas.
- Author
-
Ramirez-Limon DA, Gonzaga-Carlos N, Angulo-Lozano JC, Miranda-Symes O, and Virgen-Gutierrez F
- Abstract
Wünderlich syndrome (WS) is a spontaneous retroperitoneal hemorrhage confined to the subcapsular or perinephric space without a history of trauma. Since it is a rare condition with a significant mortality rate if not treated timely, it is essential to identify its risk factors and early clinical manifestations for a favorable outcome. Various conditions are associated, but the most common causes are benign and malignant renal neoplasms. We present a 26-year-old female with a history of tonic-clonic seizures who presented to the ED with intense abdominal pain located on the right flank with a palpable mass. Management included IV fluids and blood transfusion. She underwent a right total nephrectomy. She was later diagnosed with tuberous sclerosis. A 44-year-old female with a three-year history of right costovertebral pain and recurrent urinary tract infections that presented to the ED with acute right flank pain was diagnosed with WS secondary to an angiomyolipoma and underwent right total nephrectomy. WS is a very rare pathology that represents a diagnostic challenge for the physician. The treatment will depend on the hemodynamic condition of the patient. Active follow-up should be reserved for those who have small tumors, are asymptomatic, and have hemodynamic stability. Surgical or radiology intervention is reserved for those who are hemodynamically unstable or who have a suspicion of renal cell carcinoma., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Ramirez-Limon et al.)
- Published
- 2022
- Full Text
- View/download PDF
22. Should Psychiatry Be Consulted When Facing a Self-Inflicted Foreign Body in the Urinary Tract?
- Author
-
Angulo-Lozano JC, Gonzaga-Carlos N, Virgen-Rivera MF, Sanchez-Musi LF, Acosta-Falomir MJ, De la Cruz-Galvan R, Castillo-Del Toro IA, Magaña-Gonzalez JE, Virgen-Gutierrez F, and Jaspersen Gastelum J
- Abstract
Background On encountering a self-inflicted foreign body in the urinary tract, it is common that emergency physicians only consult the department of urology, and no further evaluations from other specialties are sought. Psychological conditions can also involve people with psychiatric disorders who perform self-harming or sexual practices. Many case reports of foreign bodies have been reported in the literature. However, there is little information regarding which specialties to consult in this situation within the emergency department (ED). Methodology This case series study gathered information on 10 cases from patients who attended the ED from 2005 to 2020 with the diagnosis of genital or lower urinary tract foreign body. Results In total, 10 patients were analyzed with a mean age of 37.3 (SD: ±14.1) years. Of the 10 patients, seven (70%) were males, and three (30%) were females. Overall, four (40%) patients presented with lower urinary tract symptoms (dysuria, tenesmus, hematuria, urinary frequency), five (50%) patients had a significant psychiatric history, and eight (80%) patients admitted having these practices for sexual gratification. Conclusions Foreign bodies in the lower urinary tract pose a significant challenge to ED physicians and urologists because some patients do not admit or do not recall inserting foreign bodies. Patients should be interrogated for mental illness, medication use, and a history of foreign bodies in the urinary tract or genitals during the initial evaluation. There is no consensus or screening method for such patients presenting to the ED. Hence, the use of complementary imaging studies and cystoscopy is fundamental for diagnosis. Further, it is essential to perform a psychiatric evaluation to diagnose or address any underlying psychiatric conditions that could cause this behavior., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Angulo-Lozano et al.)
- Published
- 2022
- Full Text
- View/download PDF
23. Detection of High-Grade Prostate Cancer With a Super High B-value (4000 s/mm2) in Diffusion-Weighted Imaging Sequences by Magnetic Resonance Imaging.
- Author
-
Acosta-Falomir MJ, Angulo-Lozano JC, Sanchez-Musi LF, Soria Céspedes D, and Fernández de Lara Barrera Y
- Abstract
Introduction: High-grade adenocarcinoma of the prostate tends to have denser glandular structures and a prominent desmoplastic reaction, which could be detected by magnetic resonance imaging (MRI) with a super-high b-value in diffusion-weighted imaging (DWI) sequence, to differentiate it from low-grade carcinomas., Objective: To evaluate the diagnostic validity of the diffusion sequence with values of b4000 s/mm2 for the diagnosis of high-grade prostate cancer (Gleason score ≥ 7)., Materials and Methods: It is a retrospective analytical study of male patients who have undergone a prostate biopsy and count with a prostate MRI with a DWI sequence of a super-high b-value (4000 s/mm2)., Results: The sensitivity of the diffusion sequence with b4000 s/mm2 values to classify as positive for prostate cancer was 57.14% as compared to biopsy. The specificity of the diffusion sequence with b4000 s/mm2 values classifying patients with prostate carcinoma as negative was 84.62%. The probability that the diffusion sequence with b4000 s/mm2 values classifies patients with prostate cancer was 80%. The probability that the diffusion sequence with b4000 s/mm2 values does not classify patients with prostate cancer was 64.71%. The proportion of patients adequately classified with prostate cancer using the diffusion sequence with b4000 s/mm2 values was 70.37%., Conclusions: The study shows that using the diffusion sequence with values of b4000 s/mm2 is an optimal value that serves as a tool to be able to decant those high-risk carcinomas with those of low risk; however, it is not a definitive method of diagnosis that could replace the performance of a biopsy. Since the study sample was limited, these results cannot be interpreted as reliable for diagnosing high-grade prostate cancer and should encourage future studies on a larger scale population to obtain significant evidence for a non-invasive diagnostic tool with a better cost-benefit for the patient., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Acosta-Falomir et al.)
- Published
- 2022
- Full Text
- View/download PDF
24. Contextualist Perspectives in the Treatment of Antisocial Behaviors and Offending: A Comparative Review of FAP, ACT, DBT, and MDT.
- Author
-
Visdómine-Lozano JC
- Subjects
- Adolescent, Antisocial Personality Disorder therapy, Humans, Psychotherapy, Acceptance and Commitment Therapy, Dialectical Behavior Therapy, Sex Offenses
- Abstract
This article presents a comparative review of the application of four contextualist therapies in the treatment of antisocial behavior and offending. The therapies reviewed are functional analytic psychotherapy (FAP), acceptance and commitment therapy (ACT), dialectical behavior therapy (DBT), and mode deactivation therapy (MDT). A descriptive and comparative review was conducted through a search carried out in both general and specific databases related to each therapy. We included a total of 44 studies treating any type of antisocial behavior or offending. Results show that these interventions have been used to treat challenging behavioral patterns, inmates' institutional behaviors, exhibitionism, at-risk adolescents' aggressive conducts, and offending behaviors performed by juveniles who committed robbery and/or serious sexual offenses. The main conclusions are that the four therapies show very positive outcomes: Although FAP and ACT have been used more sparsely, DBT and MDT have been employed in a larger number of interventions and using more controlled comparative designs. The therapeutic components that seem to be relevant to understanding in a transversal way how changes in behavior are achieved are acceptance/validation of clients' histories of neglect and abuse and clients' commitment to behaving toward their valued directions in life.
- Published
- 2022
- Full Text
- View/download PDF
25. Management of "stone pain" between the 18th and 19th centuries: A brief history of a medical prescription in the Viceroyalty of New Granada.
- Author
-
Tuta-Quintero E, Martínez-Lozano JC, Briceño-Balcázar I, Guerron-Gómez G, and Gómez-Gutiérrez A
- Subjects
- Animals, Humans, Pain, Plant Extracts therapeutic use, Prescriptions, Kidney Calculi, Malva
- Abstract
Introduction: The presence of stones in the urinary tract is a condition that has accompanied humans since ancient times. In colonial times, this condition was known as "stone pain" and its non-surgical management was based on the use of medicines derived from plants, animals and minerals., Objective: To contextualize a medical prescription used to modulate stone pain in the 18th century in the New Kingdom of Granada. Additionally, to analyze its components and evaluate the basis of its possible phytotherapeutic effects on the disease and pain., Material and Method: Document search in the Cipriano Rodríguez Santamaría Historical Archive of the Octavio Arizmendi Posada Library at Universidad de La Sabana. The document entitled "Prescription for bladder or kidney stone pain" was analyzed, and a complementary review of current scientific literature and original texts was performed with no time limits, in order to compare this prescription to related findings in the history of medicine., Results: The transcription of the source document revealed several phytotherapeutic agents such as chamomile (Matricaria recutita), lilies (Lilium lancifolium), clover (Trifolium pratense), and mallow roots (Malva sylvestris), accompanied by a large amounts of water., Conclusions: There is scientific evidence that could explain the anti-inflammatory and antioxidant effects of all plant-derived medicines used in this prescription. Abundant water intake to increase urine volume was an essential part of treatment. However, the lack of more precise data related to the prescription and the evolution of the patients makes it difficult to analyze its therapeutic efficacy., (Copyright © 2020 AEU. Published by Elsevier España, S.L.U. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
26. [Disease genotype, haplotypes, diagnosis and associated studies in sickle cell anemia].
- Author
-
Díaz-Matallana M, Márquez-Benítez Y, Martínez-Lozano JC, Briceño-Balcázar I, Benavides-Benítez E, and Bernal JE
- Subjects
- Haplotypes, Humans, Hydroxyurea therapeutic use, Hypoxia drug therapy, Anemia, Sickle Cell diagnosis, Anemia, Sickle Cell genetics
- Abstract
Sickle cell anemia is a type of hemoglobinopathy characterized by a specific mutation in the beta globin gene with the consequent generation of an unstable hemoglobin that crystallizes in a state of hypoxia. This causes a change in the structure of the red blood cell, which ends up producing vaso-occlusion with the corresponding clinical complications for the patient. Worldwide, various diagnostic tests have been developed that allow the appropriate approach to the affected patient. These include techniques for the determination of hemoglobin and the use of molecular markers, among others. There are new therapeutic alternatives to the use of hydroxyurea and L-glutamine, such as the use of gene therapy tools. The most recent experimental trials are exploring gene editing techniques.
- Published
- 2021
- Full Text
- View/download PDF
27. Seasonal marine microorganisms change neighbours under contrasting environmental conditions.
- Author
-
Lambert S, Lozano JC, Bouget FY, and Galand PE
- Subjects
- Bacteria genetics, Plankton, Seasons, Archaea genetics, Ecosystem
- Abstract
Marine picoplankton contribute to global carbon sequestration and nutrient recycling. These processes are directly related to the composition of communities, which in turn depends on microbial interactions and environmental forcing. Under regular seasonal cycles, marine communities show strong predictable patterns of annual re-occurrences, but little is known about the effect of environmental perturbation on their organization. The aim of our study was to investigate the co-occurrence patterns of planktonic picoeukaryote, bacteria and archaea under contrasting environmental conditions. The study was designed to have high sampling frequency that could match both the biological rhythm of marine microbes and the short time scale of extreme weather events. Our results show that microbial networks changed from year to year depending on conditions. In addition, individual taxa became less interconnected and changed neighbours, which revealed an unfaithful relationship between marine microorganisms. This unexpected pattern suggests possible switches between organisms that have similar specific functions, or hints at the presence of organisms that share similar environmental niches without interacting. Despite the observed annual changes, the time series showed re-occurring communities that appear to recover from perturbations. Changing co-occurrence patterns between marine microorganisms may allow the long-term stability of ecosystems exposed to contrasting meteorological events., (© 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Published
- 2021
- Full Text
- View/download PDF
28. Prenatal diagnostic techniques and IVF in patients with coagulopathies.
- Author
-
García-Lozano JC and Lozano-Arana MD
- Subjects
- Female, Genetic Counseling methods, Hemophilia A genetics, Humans, Male, Preimplantation Diagnosis methods, Blood Coagulation Disorders, Inherited genetics, Fertilization in Vitro, Genetic Testing methods, Prenatal Diagnosis methods
- Abstract
Hemophilia and other hereditary coagulopathies tend to be associated with a huge negative impact both for individuals who suffer the disease and for their families. In this respect, hemophilia carriers feel the need to make reproductive decisions which will inevitably affect their children, their families and from themselves. Genetic and reproductive counseling is of the essence to alleviate these women's distress. Prenatal diagnosis and preimplantation genetic diagnosis (PGD) allow couples at high-risk of transmitting genetic diseases like hemophilia and other hereditary coagulopathies to prevent the birth of children with the disease. The main difference between prenatal diagnosis and PGD is related to the time at which diagnosis is made. Prenatal diagnosis is done when the woman is pregnant, and both the performance of the technique and its result can affect the course of pregnancy. PGD is a diagnostic procedure in which embryos created in vitro are analyzed for genetic defects before being transferred to the uterus. Performance of both prenatal diagnosis and PGD is subject to a few prerequisites: the establishment of an exact clinical diagnosis, an understanding of the parental genetic alterations that are responsible for the disease and technical feasibility of genetic diagnosis. These couples should be provided with complete, up-to-date and easy-to-understand information., (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
29. Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
- Author
-
Faktorová D, Nisbet RER, Fernández Robledo JA, Casacuberta E, Sudek L, Allen AE, Ares M Jr, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget FY, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano JC, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Novák Vanclová AMG, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Jason Smith G, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, von Dassow P, von der Haar T, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, and Lukeš J
- Subjects
- Biodiversity, Ecosystem, Environment, Eukaryota classification, Species Specificity, DNA administration & dosage, Eukaryota physiology, Green Fluorescent Proteins metabolism, Marine Biology, Models, Biological, Transformation, Genetic
- Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
- Published
- 2020
- Full Text
- View/download PDF
30. Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
- Author
-
Faktorová D, Nisbet RER, Fernández Robledo JA, Casacuberta E, Sudek L, Allen AE, Ares M Jr, Aresté C, Balestreri C, Barbrook AC, Beardslee P, Bender S, Booth DS, Bouget FY, Bowler C, Breglia SA, Brownlee C, Burger G, Cerutti H, Cesaroni R, Chiurillo MA, Clemente T, Coles DB, Collier JL, Cooney EC, Coyne K, Docampo R, Dupont CL, Edgcomb V, Einarsson E, Elustondo PA, Federici F, Freire-Beneitez V, Freyria NJ, Fukuda K, García PA, Girguis PR, Gomaa F, Gornik SG, Guo J, Hampl V, Hanawa Y, Haro-Contreras ER, Hehenberger E, Highfield A, Hirakawa Y, Hopes A, Howe CJ, Hu I, Ibañez J, Irwin NAT, Ishii Y, Janowicz NE, Jones AC, Kachale A, Fujimura-Kamada K, Kaur B, Kaye JZ, Kazana E, Keeling PJ, King N, Klobutcher LA, Lander N, Lassadi I, Li Z, Lin S, Lozano JC, Luan F, Maruyama S, Matute T, Miceli C, Minagawa J, Moosburner M, Najle SR, Nanjappa D, Nimmo IC, Noble L, Novák Vanclová AMG, Nowacki M, Nuñez I, Pain A, Piersanti A, Pucciarelli S, Pyrih J, Rest JS, Rius M, Robertson D, Ruaud A, Ruiz-Trillo I, Sigg MA, Silver PA, Slamovits CH, Jason Smith G, Sprecher BN, Stern R, Swart EC, Tsaousis AD, Tsypin L, Turkewitz A, Turnšek J, Valach M, Vergé V, von Dassow P, von der Haar T, Waller RF, Wang L, Wen X, Wheeler G, Woods A, Zhang H, Mock T, Worden AZ, and Lukeš J
- Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2020
- Full Text
- View/download PDF
31. How the distribution coefficient of 238 U in natural soils is affected by the method used to obtain the soil solution and its dependency on structural characteristics.
- Author
-
Blanco Rodríguez P, Lozano JC, and Vera Tomé F
- Subjects
- Hydrogen-Ion Concentration, Radiochemistry, Water analysis, Environmental Monitoring methods, Soil chemistry, Soil Pollutants, Radioactive analysis, Uranium analysis
- Abstract
A systematic study on desorption of uranium in a natural soil has been carried out to reduce the level of uncertainty associated with the method employed to determine the values of the distribution coefficient (K
d ). Generally, the operating method used to extract and analyze the soil solution determines the Kd values. Here, the centrifugation method has been used to obtain soil solution extracts. Several procedural parameters have been considered such as incubation time, the level of soil moisture relative to saturation (saturation degree) and centrifugation speed (equivalent to effective suction). In order to analyze the influence of soil structural characteristics, this study considers three grain-size fractions of soil: loamy coarse sand, loamy fine sand, and loam, all of which are obtained from a natural soil collected in a uranium mineralized area. Our results indicate that neither incubation time nor centrifugation speed influence the determination of Kd for uranium. The results also indicate that the level of soil moisture is the most important factor for determining238 U-Kd . It has been shown that the influence of moisture on Kd also depends on the structural characteristic of the soil. For the loamy coarse sand subsample, the moisture level during the incubation period showed a significant influence on the Kd . In addition, through the use of regression analysis, the pH was identified as the cofactor with the greatest influence on Kd of uranium., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
32. Prescription to reduce heart palpitations, a mid-18 th century prescription in the New Kingdom of Granada.
- Author
-
Uribe-Vergara J, Briceño-Balcázar I, Martínez-Lozano JC, Pacheco-Cuentas C, Tuta-Quintero E, Rueda-Rodríguez A, and Gómez-Gutiérrez A
- Subjects
- Arrhythmias, Cardiac drug therapy, History, 18th Century, History, 19th Century, Humans, Plant Preparations pharmacology, Arrhythmias, Cardiac history, Plant Preparations history, Plants, Medicinal chemistry
- Abstract
The human being, throughout history, has used plants to prevent and cure diseases. It is important to know that for a long time, the mechanism through which those plants worked was unknown, making herbal medicine a purely empirical science. Medical prescriptions in the 19
th century in the Kingdom of Nueva Granada were considered a significant medical advance as a result of knowledge and medical practices in the old continent. Medical literature of the time achieved, despite the lack of studies, the development of new schemes with exact dosages and new therapeutic possibilities. The medical prescription presented in this article was used in the management of heart palpitations, a frequent symptom nowadays, described as thoracic and/or neck beating, underlying various cardiac and non-cardiac diseases. The recipe for the palpitations of the historical archive "Cipriano Rodríguez Santa María" is a mixture of herbal agents that, as reviewed in the medical literature, showed to have anti-inflammatory, anxiolytic, and antioxidant effects, among others, allowing a beneficial effect on cardiac palpitations. Due to the lack of information on the posology, safety in its use, contraindications, and possible adverse effects, its potential use should have been underestimated at that time for the control of palpitations or as phytochemical agents directed to treat diseases causing this symptom., (Copyright: © 2020 Permanyer.)- Published
- 2020
- Full Text
- View/download PDF
33. Prescription to reduce heart palpitations, a mid-18th century prescription in the New Kingdom of Granada.
- Author
-
Uribe-Vergara J, Briceño-Balcázar I, Martínez-Lozano JC, Pacheco-Cuentas C, Tuta-Quintero E, Rueda-Rodríguez A, and Gómez-Gutiérrez A
- Subjects
- Arrhythmias, Cardiac history, History, 18th Century, History, 19th Century, Humans, Plant Preparations administration & dosage, Plant Preparations pharmacology, Arrhythmias, Cardiac drug therapy, Plant Preparations history, Plants, Medicinal chemistry
- Abstract
The human being, throughout history, has used plants to prevent and cure diseases. It is important to know that for a long time, the mechanism through which those plants worked was unknown, making herbal medicine a purely empirical science. Medical prescriptions in the 19th century in the Kingdom of Nueva Granada were considered a significant medical advance, as a result of knowledge and medical practices in the old continent. Medical literature of the time achieved, despite the lack of studies, the development of new schemes with exact dosages and new therapeutic possibilities. The medical prescription presented in this article was used in the management of heart palpitations, a frequent symptom nowadays, described as thoracic and/or neck beating, underlying various cardiac and non-cardiac diseases. The recipe for the palpitations of the historical archive "Cipriano Rodríguez Santa María" is a mixture of herbal agents that, as reviewed in the medical literature, showed to have anti-inflammatory, anxiolytic, and antioxidant effects, among others, allowing a beneficial effect on cardiac palpitations. Due to the lack of information on the posology, safety in its use, contraindications and possible adverse effects, its potential use should have been underestimated at that time for the control of palpitations or as phytochemical agents directed to treat diseases causing this symptom., (Copyright: © 2020 Permanyer.)
- Published
- 2020
- Full Text
- View/download PDF
34. Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34 + Cell Viability after Thawing.
- Author
-
Vanegas D, Galindo CC, Páez-Gutiérrez IA, González-Acero LX, Medina-Valderrama PT, Lozano JC, Camacho-Rodríguez B, and Perdomo-Arciniegas AM
- Subjects
- Alleles, Antigens, CD34 metabolism, Cell Survival genetics, Cord Blood Stem Cell Transplantation, Cryopreservation, Hematopoietic Stem Cells cytology, Humans, Cell Communication genetics, Erythrocytes metabolism, Fetal Blood cytology, HLA Antigens genetics, Hematopoietic Stem Cells metabolism
- Abstract
Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34
+ viability after thawing of UCB units matched for two different patients ( p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model ( n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C ( R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it., Competing Interests: The authors declare no conflict of interest.- Published
- 2019
- Full Text
- View/download PDF
35. Rhythmicity of coastal marine picoeukaryotes, bacteria and archaea despite irregular environmental perturbations.
- Author
-
Lambert S, Tragin M, Lozano JC, Ghiglione JF, Vaulot D, Bouget FY, and Galand PE
- Subjects
- Archaea classification, Archaea physiology, Bacteria genetics, Ecosystem, Eukaryota classification, Mediterranean Sea, Phytoplankton growth & development, Seasons, Archaea genetics, Bacteria classification, Eukaryota genetics, Seawater microbiology
- Abstract
Seasonality in marine microorganisms has been classically observed in phytoplankton blooms, and more recently studied at the community level in prokaryotes, but rarely investigated at the scale of individual microbial taxa. Here we test if specific marine eukaryotic phytoplankton, bacterial and archaeal taxa display yearly rhythms at a coastal site impacted by irregular environmental perturbations. Our seven-year study in the Bay of Banyuls (North Western Mediterranean Sea) shows that despite some fluctuating environmental conditions, many microbial taxa displayed significant yearly rhythms. The robust rhythmicity was found in both autotrophs (picoeukaryotes and cyanobacteria) and heterotrophic prokaryotes. Sporadic meteorological events and irregular nutrient supplies did, however, trigger the appearance of less common non-rhythmic taxa. Among the environmental parameters that were measured, the main drivers of rhythmicity were temperature and day length. Seasonal autotrophs may thus be setting the pace for rhythmic heterotrophs. Similar environmental niches may be driving seasonality as well. The observed strong association between Micromonas and SAR11, which both need thiamine precursors for growth, could be a first indication that shared nutritional niches may explain some rhythmic patterns of co-occurrence.
- Published
- 2019
- Full Text
- View/download PDF
36. Influence of soil conditions on the distribution coefficients of 226 Ra in natural soils.
- Author
-
Blanco Rodríguez P, Lozano JC, Vera Tomé F, Prieto C, and Medeiros A
- Subjects
- Particle Size, Radium analysis, Silicon Dioxide chemistry, Soil chemistry, Soil Pollutants, Radioactive analysis
- Abstract
In order to clarify some of the assumptions and approximations about the use of the distribution coefficient K
d for226 Ra in soils, a systematic study has been performed using centrifugation to extract the soil solution. The separated fractions of the soil solution have different kinetics with respect to the sorption process in the soil, which may in turn condition the final chemical composition and even the speciation of the radionuclides in solution. In the experimental design of this study three factors were considered: the moisture level in the incubation process, incubation time and the speed of centrifugation. Also, three levels were chosen for each factor. In order to analyze the influence of the structural characteristics of the soil, this study was performed with three textural fractions: coarse sand, fine sand, and silt and clay, obtained from an only soil. Also, the soil was naturally enriched with radionuclides of the238 U series. An analysis of variance (ANOVA) was performed in order to assess the influence of the factors studied on the distribution coefficient of226 Ra. The results indicate that different behaviors can be observed depending on the structural characteristic of the soil. In the case of particle size, the soil with the largest grain size showed that the incubation process parameters influence the equilibrium level achieved, while in the case of the smallest edaphic particles, radium is not homogeneously distributed in the soil solution and the Kd value is dependent on the speed of centrifugation., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
37. Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus.
- Author
-
Guyon JB, Vergé V, Schatt P, Lozano JC, Liennard M, and Bouget FY
- Subjects
- Biotechnology instrumentation, Chlorophyta genetics, Lipids, Microalgae genetics, Photobioreactors, Phylogeny, Salinity, Temperature, Biotechnology methods, Carotenoids biosynthesis, Chlorophyta metabolism, Microalgae metabolism
- Abstract
Microalgae are promising sources for the sustainable production of compounds of interest for biotechnologies. Compared to higher plants, microalgae have a faster growth rate and can be grown in industrial photobioreactors. The microalgae biomass contains specific metabolites of high added value for biotechnology such as lipids, polysaccharides or carotenoid pigments. Studying carotenogenesis is important for deciphering the mechanisms of adaptation to stress tolerance as well as for biotechnological production. In recent years, the picoeukaryote Ostreococcus tauri has emerged as a model organism thanks to the development of powerful genetic tools. Several strains of Ostreococcus isolated from different environments have been characterized with respect to light response or iron requirement. We have compared the carotenoid contents and growth rates of strains of Ostreococcus (OTTH595, RCC802 and RCC809) under a wide range of light, salinity and temperature conditions. Carotenoid profiles and productivities varied in a strain-specific and stress-dependent manner. Our results also illustrate that phylogenetically related microalgal strains originating from different ecological niches present specific interests for the production of specific molecules under controlled culture conditions., Competing Interests: The authors declare no conflict of interest.
- Published
- 2018
- Full Text
- View/download PDF
38. Mazinger, a γ-ray spectrometry system of high efficiency and very low background for paloeoclimate applications.
- Author
-
Quintana B, Pedrosa C, Bombín R, Martín S, and Lozano JC
- Abstract
The study of changes in climate on the scale of the entire history of Earth requires absolute dating methods that cover the total time period.
230 Th and234 U disequilibria, as well as231 Pa and235 U, have shown as good candidates to provide ages older than 50ky. Therefore, they are the basis for a method that allows extending the14 C dating up to 500ky or even further. The wide variety of samples, their high number and the fact that several radionuclides are needed to obtain samples' chronology make γ spectrometry to be a suitable technique in this task. However, its low efficiency and high background, therefore, high detection limits in comparison with α spectrometry, need to be improved to become a real alternative in samples with very low activity levels. With this purpose, Mazinger, a new γ spectrometry system based on two HPGe detectors, which are surrounded by active and passive shieldings and placed 12m underground, has been set up in the Ionizing Radiation Laboratory of the University of Salamanca. In this work, the Mazinger's performance is studied at the full energy range useful for natural radionuclides. In composite detectors, energy resolution is a consequence of the electronics adjustments, which have to enable the raw spectra to be summed without resolution worsening. Therefore, the method used to set up electronics and add spectra is described. Detector background is also analysed and compared with the one of a single low-background HPGe detector as a function of the different shielding elements, obtaining gross values of order 10-4 cps. Efficiency and figure of merit are obtained for the main natural radionuclides in marine sediments belonging to the Mediterranean MD99-2348 core. Special focus is made on the230 Th determination whose results are compared with the ones provided by α spectrometry. Results show that Mazinger´s performance, in terms of figure of merit, is four times better than the one of a low-level background HPGe detector, specially suited to detect natural radionuclides, being comparable to the performance of α spectrometry in the230 Th determination, which is the radionuclide of lower γ intensity, and doing Mazinger a first class in radiometry of natural radionuclides., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
39. Acclimation of a low iron adapted Ostreococcus strain to iron limitation through cell biomass lowering.
- Author
-
Botebol H, Lelandais G, Six C, Lesuisse E, Meng A, Bittner L, Lecrom S, Sutak R, Lozano JC, Schatt P, Vergé V, Blain S, and Bouget FY
- Subjects
- Biomass, Chlorophyta growth & development, Gene Expression Profiling, Acclimatization, Chlorophyta drug effects, Chlorophyta physiology, Iron metabolism, Trace Elements metabolism
- Abstract
Iron is an essential micronutrient involved in many biological processes and is often limiting for primary production in large regions of the World Ocean. Metagenomic and physiological studies have identified clades or ecotypes of marine phytoplankton that are specialized in iron depleted ecological niches. Although less studied, eukaryotic picophytoplankton does contribute significantly to primary production and carbon transfer to higher trophic levels. In particular, metagenomic studies of the green picoalga Ostreococcus have revealed the occurrence of two main clades distributed along coast-offshore gradients, suggesting niche partitioning in different nutrient regimes. Here, we present a study of the response to iron limitation of four Ostreococcus strains isolated from contrasted environments. Whereas the strains isolated in nutrient-rich waters showed high iron requirements, the oceanic strains could cope with lower iron concentrations. The RCC802 strain, in particular, was able to maintain high growth rate at low iron levels. Together physiological and transcriptomic data indicate that the competitiveness of RCC802 under iron limitation is related to a lowering of iron needs though a reduction of the photosynthetic machinery and of protein content, rather than to cell size reduction. Our results overall suggest that iron is one of the factors driving the differentiation of physiologically specialized Ostreococcus strains in the ocean.
- Published
- 2017
- Full Text
- View/download PDF
40. Use of plankton-derived vitamin B1 precursors, especially thiazole-related precursor, by key marine picoeukaryotic phytoplankton.
- Author
-
Paerl RW, Bouget FY, Lozano JC, Vergé V, Schatt P, Allen EE, Palenik B, and Azam F
- Subjects
- Chlorophyta genetics, Plankton metabolism, Pyrimidines, Eukaryota metabolism, Plankton classification, Thiamine chemistry, Thiamine metabolism, Thiazoles metabolism
- Abstract
Several cosmopolitan marine picoeukaryotic phytoplankton are B1 auxotrophs requiring exogenous vitamin B1 or precursor to survive. From genomic evidence, representatives of picoeukaryotic phytoplankton (Ostreococcus and Micromonas spp.) were predicted to use known thiazole and pyrimidine B1 precursors to meet their B1 demands, however, recent culture-based experiments could not confirm this assumption. We hypothesized these phytoplankton strains could grow on precursors alone, but required a thiazole-related precursor other the well-known and extensively tested 4-methyl-5-thiazoleethanol. This hypothesis was tested using bioassays and co-cultures of picoeukaryotic phytoplankton and bacteria. We found that specific B1-synthesizing proteobacteria and phytoplankton are sources of a yet-to-be chemically identified thiazole-related precursor(s) that, along with pyrimidine B1 precursor 4-amino-5-hydroxymethyl-2-methylpyrimidine, can support growth of Ostreococcus spp. (also Micromonas spp.) without B1. We additionally found that the B1-synthesizing plankton do not require contact with picoeukaryotic phytoplankton cells to produce thiazole-related precursor(s). Experiments with wild-type and genetically engineered Ostreococcus lines revealed that the thiazole kinase, ThiM, is required for growth on precursors, and that thiazole-related precursor(s) accumulate to appreciable levels in the euphotic ocean. Overall, our results point to thiazole-related B1 precursors as important micronutrients promoting the survival of abundant phytoplankton influencing surface ocean production and biogeochemical cycling.
- Published
- 2017
- Full Text
- View/download PDF
41. Influence of soil structure on the "F v approach" applied to 238 U and 226 Ra.
- Author
-
Blanco Rodríguez P, Vera Tomé F, and Lozano JC
- Subjects
- Asteraceae metabolism, Fabaceae metabolism, Plants radiation effects, Poaceae metabolism, Radium metabolism, Soil Pollutants, Radioactive adverse effects, Soil Pollutants, Radioactive metabolism, Uranium metabolism, Plants metabolism, Radium analysis, Silicon Dioxide chemistry, Soil chemistry, Soil Pollutants, Radioactive analysis, Uranium analysis
- Abstract
The soil-to-plant transfer factors were determined in a granitic area for the two long-lived uranium series radionuclides
238 U and226 Ra. With the aim to identify a physical fraction of soil whose concentration correlates linearly with the plant concentration, the soil compartment was analyzed in various stages. An initial study identified the soil compartments as being either bulk soil or its labile fraction. The bulk soil was subsequently divided into three granulometric fractions consisting of: coarse sand, fine sand, and silt and clay. The soil-to-plant transfer of radionuclides for each of these three texture fractions was analyzed. Lastly, the labile fraction was extracted from each textural part, and the activity concentration of the radionuclides238 U and226 Ra was measured. In order to assess the influence of soil texture on the soil-to-plant transfer process, we sought to identify possible correlations between the activity concentration in the plant compartment and those found in the different fractions within each soil compartment. The results showed that the soil-to-plant transfer process for uranium and radium depends on soil grain size, where the results for uranium showed a linear relationship between the activity concentration of uranium in the plant and the fine soil fraction. In contrast, a linear relation between the activity concentration of radium in the plant and the soil coarse-sand fraction was observed. Additionally, the presence of phosphate and calcium in the soil of all of the compartments studied affected the soil-to-plant transfer of uranium and radium, respectively., (Copyright © 2016 Elsevier Ltd. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF
42. Preimplantation Genetic Diagnosis for Myotonic Dystrophy Type 1 and Analysis of the Effect of the Disease on the Reproductive Outcome of the Affected Female Patients.
- Author
-
Fernández RM, Lozano-Arana MD, Sánchez B, Peciña A, García-Lozano JC, Borrego S, and Antiñolo G
- Subjects
- Adult, Cohort Studies, Female, Haplotypes genetics, Humans, Male, Microsatellite Repeats genetics, Pregnancy, Myotonic Dystrophy diagnosis, Myotonic Dystrophy epidemiology, Myotonic Dystrophy genetics, Pregnancy Outcome epidemiology, Pregnancy Outcome genetics, Preimplantation Diagnosis
- Abstract
Myotonic dystrophy type 1 (DM1) is the most common adult muscular dystrophy and presents an autosomal dominant inheritance. A reproductive option for the families affected is preimplantation genetic diagnosis (PGD). One limitation of this option is the nonoptimal response to ovarian stimulation of the women with DM1, although controversial results exist regarding this subject. In this study, we have analyzed the results of the PGD program applied to DM1 at our institution. A total of 35 couples have been included in our program since 2010, and 59 cycles have been performed. The percentage of transfers per cycle was 64.4% and the live birth rate per cycle was 18.6%. Interestingly, statistically significant differences were observed for the clinical results in the group of couples with an affected female versus the group with an affected male or versus a group of couples with different referral reasons. Specifically, both the percentage of mature oocytes out of the total oocytes retrieved and the percentage of fertilization were considerably lower in the group of DM1 females. Our findings would suggest the possibility of achieving less favourable PGD outcomes in women with DM1 in comparison with other pathologies, although the underlying mechanism remains unknown.
- Published
- 2017
- Full Text
- View/download PDF
43. Ostreococcus tauri is a new model green alga for studying iron metabolism in eukaryotic phytoplankton.
- Author
-
Lelandais G, Scheiber I, Paz-Yepes J, Lozano JC, Botebol H, Pilátová J, Žárský V, Léger T, Blaiseau PL, Bowler C, Bouget FY, Camadro JM, Sutak R, and Lesuisse E
- Subjects
- Adaptation, Biological, Chlorophyta classification, Chlorophyta genetics, Cluster Analysis, Copper metabolism, Eukaryota genetics, Gene Expression Profiling, Gene Expression Regulation radiation effects, High-Throughput Nucleotide Sequencing, Homeostasis, Iron Compounds metabolism, Oxidation-Reduction, Photoperiod, Phylogeny, Phytoplankton genetics, Plant Proteins genetics, Plant Proteins metabolism, Signal Transduction, Stress, Physiological, Transcriptome, Chlorophyta metabolism, Eukaryota metabolism, Iron metabolism, Phytoplankton metabolism
- Abstract
Background: Low iron bioavailability is a common feature of ocean surface water and therefore micro-algae developed original strategies to optimize iron uptake and metabolism. The marine picoeukaryotic green alga Ostreococcus tauri is a very good model for studying physiological and genetic aspects of the adaptation of the green algal lineage to the marine environment: it has a very compact genome, is easy to culture in laboratory conditions, and can be genetically manipulated by efficient homologous recombination. In this study, we aimed at characterizing the mechanisms of iron assimilation in O. tauri by combining genetics and physiological tools. Specifically, we wanted to identify and functionally characterize groups of genes displaying tightly orchestrated temporal expression patterns following the exposure of cells to iron deprivation and day/night cycles, and to highlight unique features of iron metabolism in O. tauri, as compared to the freshwater model alga Chalamydomonas reinhardtii., Results: We used RNA sequencing to investigated the transcriptional responses to iron limitation in O. tauri and found that most of the genes involved in iron uptake and metabolism in O. tauri are regulated by day/night cycles, regardless of iron status. O. tauri lacks the classical components of a reductive iron uptake system, and has no obvious iron regulon. Iron uptake appears to be copper-independent, but is regulated by zinc. Conversely, iron deprivation resulted in the transcriptional activation of numerous genes encoding zinc-containing regulation factors. Iron uptake is likely mediated by a ZIP-family protein (Ot-Irt1) and by a new Fea1-related protein (Ot-Fea1) containing duplicated Fea1 domains. The adaptation of cells to iron limitation involved an iron-sparing response tightly coordinated with diurnal cycles to optimize cell functions and synchronize these functions with the day/night redistribution of iron orchestrated by ferritin, and a stress response based on the induction of thioredoxin-like proteins, of peroxiredoxin and of tesmin-like methallothionein rather than ascorbate. We briefly surveyed the metabolic remodeling resulting from iron deprivation., Conclusions: The mechanisms of iron uptake and utilization by O. tauri differ fundamentally from those described in C. reinhardtii. We propose this species as a new model for investigation of iron metabolism in marine microalgae.
- Published
- 2016
- Full Text
- View/download PDF
44. Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton.
- Author
-
Botebol H, Lesuisse E, Šuták R, Six C, Lozano JC, Schatt P, Vergé V, Kirilovsky A, Morrissey J, Léger T, Camadro JM, Gueneugues A, Bowler C, Blain S, and Bouget FY
- Subjects
- Blotting, Western, Chemical Precipitation, Ferritins genetics, Gene Expression Regulation drug effects, Gene Expression Regulation radiation effects, Iron pharmacology, Iron-Binding Proteins metabolism, Kinetics, Light, Mass Spectrometry, Microbial Viability drug effects, Microbial Viability radiation effects, Phytoplankton drug effects, Phytoplankton genetics, Phytoplankton growth & development, Phytoplankton metabolism, Transcriptome genetics, Circadian Rhythm drug effects, Circadian Rhythm genetics, Circadian Rhythm radiation effects, Ferritins metabolism, Homeostasis drug effects, Homeostasis genetics, Homeostasis radiation effects, Iron metabolism, Seawater microbiology
- Abstract
In large regions of the open ocean, iron is a limiting resource for phytoplankton. The reduction of iron quota and the recycling of internal iron pools are among the diverse strategies that phytoplankton have evolved to allow them to grow under chronically low ambient iron levels. Phytoplankton species also have evolved strategies to cope with sporadic iron supply such as long-term storage of iron in ferritin. In the picophytoplanktonic species Ostreococcus we report evidence from observations both in the field and in laboratory cultures that ferritin and the main iron-binding proteins involved in photosynthesis and nitrate assimilation pathways show opposite diurnal expression patterns, with ferritin being maximally expressed during the night. Biochemical and physiological experiments using a ferritin knock-out line subsequently revealed that this protein plays a central role in the diel regulation of iron uptake and recycling and that this regulation of iron homeostasis is essential for cell survival under iron limitation.
- Published
- 2015
- Full Text
- View/download PDF
45. Clinical and Technical Overview of Preimplantation Genetic Diagnosis for Fragile X Syndrome: Experience at the University Hospital Virgen del Rocio in Spain.
- Author
-
Fernández RM, Peciña A, Lozano-Arana MD, Sánchez B, García-Lozano JC, Borrego S, and Antiñolo G
- Subjects
- Adult, Birth Rate, Embryo Transfer, Female, Fragile X Syndrome genetics, Fragile X Syndrome pathology, Heterozygote, Hospitals, University, Humans, Pregnancy, Primary Ovarian Insufficiency genetics, Primary Ovarian Insufficiency pathology, Spain, Fragile X Syndrome diagnosis, Genetic Testing, Preimplantation Diagnosis, Primary Ovarian Insufficiency diagnosis
- Abstract
Fragile X syndrome (FXS) accounts for about one-half of cases of X-linked intellectual disability and is the most common monogenic cause of mental impairment. Reproductive options for the FXS carriers include preimplantation genetic diagnosis (PGD). However, this strategy is considered by some centers as wasteful owing to the high prevalence of premature ovarian failure in FXS carriers and the difficulties in genetic diagnosis of the embryos. Here we present the results of our PGD Program applied to FXS, at the Department of Genetics, Reproduction and Fetal Medicine of the University Hospital Virgen del Rocío in Seville. A total of 11 couples have participated in our PGD Program for FXS since 2010. Overall, 15 cycles were performed, providing a total of 43 embryos. The overall percentage of transfers per cycle was 46.67% and the live birth rate per cycle was 13.33%. As expected, these percentages are considerably lower than the ones obtained in PGD for other pathologies. Our program resulted in the birth of 3 unaffected babies of FXS for 2 of the 11 couples (18.2%) supporting that, despite the important drawbacks of PGD for FXS, efforts should be devoted in offering this reproductive option to the affected families.
- Published
- 2015
- Full Text
- View/download PDF
46. Experience of Preimplantation Genetic Diagnosis for Hemophilia at the University Hospital Virgen Del Rocío in Spain: Technical and Clinical Overview.
- Author
-
Fernández RM, Peciña A, Sánchez B, Lozano-Arana MD, García-Lozano JC, Pérez-Garrido R, Núñez R, Borrego S, and Antiñolo G
- Subjects
- Adult, Embryo, Mammalian physiology, Female, Humans, In Situ Hybridization, Fluorescence, Male, Microsatellite Repeats genetics, Polymerase Chain Reaction, Pregnancy, Spain, Genetic Testing methods, Hemophilia A diagnosis, Hemophilia A genetics, Hemophilia B diagnosis, Hemophilia B genetics, Hospitals, University, Preimplantation Diagnosis methods
- Abstract
Hemophilia A and B are the most common hereditary hemorrhagic disorders, with an X-linked mode of inheritance. Reproductive options for the families affected with hemophilia, aiming at the prevention of the birth of children with severe coagulation disorders, include preimplantation genetic diagnosis (PGD). Here we present the results of our PGD Program applied to hemophilia, at the Department of Genetics, Reproduction and Fetal Medicine of the University Hospital Virgen del Rocío in Seville. A total of 34 couples have been included in our program since 2005 (30 for hemophilia A and 4 for hemophilia B). Overall, 60 cycles were performed, providing a total of 508 embryos. The overall percentage of transfers per cycle was 81.7% and the live birth rate per cycle ranged from 10.3 to 24.1% depending on the methodological approach applied. Although PGD for hemophilia can be focused on gender selection of female embryos, our results demonstrate that methodological approaches that allow the diagnosis of the hemophilia status of every embryo have notorious advantages. Our PGD Program resulted in the birth of 12 healthy babies for 10 out of the 34 couples (29.4%), constituting a relevant achievement for the Spanish Public Health System within the field of haematological disorders.
- Published
- 2015
- Full Text
- View/download PDF
47. Determination and inference of eukaryotic transcription factor sequence specificity.
- Author
-
Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, Najafabadi HS, Lambert SA, Mann I, Cook K, Zheng H, Goity A, van Bakel H, Lozano JC, Galli M, Lewsey MG, Huang E, Mukherjee T, Chen X, Reece-Hoyes JS, Govindarajan S, Shaulsky G, Walhout AJM, Bouget FY, Ratsch G, Larrondo LF, Ecker JR, and Hughes TR
- Subjects
- Arabidopsis metabolism, Chromatin Immunoprecipitation, Humans, Polymorphism, Single Nucleotide, Promoter Regions, Genetic, Protein Binding, Quantitative Trait Loci, Arabidopsis genetics, Nucleotide Motifs, Sequence Analysis, DNA, Transcription Factors metabolism
- Abstract
Transcription factor (TF) DNA sequence preferences direct their regulatory activity, but are currently known for only ∼1% of eukaryotic TFs. Broadly sampling DNA-binding domain (DBD) types from multiple eukaryotic clades, we determined DNA sequence preferences for >1,000 TFs encompassing 54 different DBD classes from 131 diverse eukaryotes. We find that closely related DBDs almost always have very similar DNA sequence preferences, enabling inference of motifs for ∼34% of the ∼170,000 known or predicted eukaryotic TFs. Sequences matching both measured and inferred motifs are enriched in chromatin immunoprecipitation sequencing (ChIP-seq) peaks and upstream of transcription start sites in diverse eukaryotic lineages. SNPs defining expression quantitative trait loci in Arabidopsis promoters are also enriched for predicted TF binding sites. Importantly, our motif "library" can be used to identify specific TFs whose binding may be altered by human disease risk alleles. These data present a powerful resource for mapping transcriptional networks across eukaryotes., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
48. Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryote Ostreococcus.
- Author
-
Lozano JC, Schatt P, Botebol H, Vergé V, Lesuisse E, Blain S, Carré IA, and Bouget FY
- Subjects
- Algal Proteins genetics, Ferritins genetics, Gene Knock-In Techniques, Gene Knockout Techniques, Genes, Reporter, Genome, Plant, Luciferases genetics, Nitrate Reductase genetics, Transformation, Genetic, Chlorophyta genetics, Gene Targeting methods, Homologous Recombination
- Abstract
With fewer than 8000 genes and a minimalist cellular organization, the green picoalga Ostreococcus tauri is one of the simplest photosynthetic eukaryotes. Ostreococcus tauri contains many plant-specific genes but exhibits a very low gene redundancy. The haploid genome is extremely dense with few repeated sequences and rare transposons. Thanks to the implementation of genetic transformation and vectors for inducible overexpression/knockdown this picoeukaryotic alga has emerged in recent years as a model organism for functional genomics analyses and systems biology. Here we report the development of an efficient gene targeting technique which we use to knock out the nitrate reductase and ferritin genes and to knock in a luciferase reporter in frame to the ferritin native protein. Furthermore, we show that the frequency of insertion by homologous recombination is greatly enhanced when the transgene is designed to replace an existing genomic insertion. We propose that a natural mechanism based on homologous recombination may operate to remove inserted DNA sequences from the genome., (© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.)
- Published
- 2014
- Full Text
- View/download PDF
49. Evolution of vegetation and soil nutrients after uranium mining in Los Ratones mine (Cáceres, Spain).
- Author
-
Pérez-Fernández MA, Vera-Tomé F, Blanco-Rodríguez MP, and Lozano JC
- Subjects
- Environmental Restoration and Remediation, Hydrogen-Ion Concentration, Mining, Nitrogen analysis, Phosphorus analysis, Radioisotopes analysis, Seasons, Soil Pollutants, Radioactive analysis, Spain, Uranium, Biodiversity, Plants classification, Soil chemistry
- Abstract
The evolution of vegetation structure following mine rehabilitation is rather scarce in the literature. The concentration of long-lived radionuclides of the (238)U series might have harmful effects on living organisms. We studied soil properties and the natural vegetation occurring along a gradient in Los Ratones, an area rehabilitated after uranium mining located in Cáceres, Spain. Soil and vegetation were sampled seasonally and physical and chemical properties of soil were analysed, including natural isotopes of (238)U, (230)Th, (226)Ra and (210)Pb. Species richness, diversity, evenness and plant cover were estimated and correlated in relation to soil physical and chemical variables. The location of the sampling sites along a gradient had a strong explanatory effect on the herbaceous species, as well as the presence of shrubs and trees. Seasonal effects of the four natural isotopes were observed in species richness, species diversity and plant cover; these effects were directly related to the pH values in the soil, this being the soil property that most influences the plant distribution. Vegetation in the studied area resembles that of the surroundings, thus proving that the rehabilitation carried out in Los Ratones mine was successful in terms of understorey cover recovery.
- Published
- 2014
- Full Text
- View/download PDF
50. Transcriptional versus non-transcriptional clocks: a case study in Ostreococcus.
- Author
-
Bouget FY, Lefranc M, Thommen Q, Pfeuty B, Lozano JC, Schatt P, Botebol H, and Vergé V
- Subjects
- Circadian Rhythm genetics, Genomics methods, Histidine Kinase, Marine Biology, Protein Kinases metabolism, Biological Clocks genetics, Chlorophyta genetics, Circadian Rhythm physiology, Models, Biological, Photoreceptors, Plant genetics, Transcription Factors genetics
- Abstract
Circadian rhythms are ubiquitous on earth from cyanobacteria to land plants and animals. Circadian clocks are synchronized to the day/night cycle by environmental factors such as light and temperature. In eukaryotes, clocks rely on complex gene regulatory networks involving transcriptional regulation but also post-transcriptional and post-translational regulations. In multicellular organisms clocks are found at multiple levels from cells to organs and whole organisms, making the study of clock mechanisms more complex. In recent years the picoalga Ostreococcus has emerged as a new circadian model organism thanks to its reduced gene redundancy and its minimalist cellular organization. A simplified version of the "green" plant clock, involving the master clock genes TOC1 and CCA1, has been revealed when the functional genomics and mathematical model approaches were combined. Specific photoreceptors such as a blue light sensing LOV histidine kinase mediate light input to the Ostreococcus clock. Non-transcriptional redox rhythms have also been identified recently in Ostreococcus and human red blood cells. This review highlights the progress made recently in the understanding of circadian clock architecture and function in Ostreococcus in the context of the marine environment., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.