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Additional file 1: Table S1. Primer sequences for RT-qPCR.

Chao Han,* Bin Xu,* Lin Zhou, Long Li, Chao Lu, Guo-Peng Yu, Yu-Shan Liu Department of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yu-Shan LiuDepartment of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, No. 639 Zhizaoju Road, Shanghai 200011, People’s Republic of ChinaTel +86-13701909308Email YushanLiu42@outlook.comBackground: Long non-coding RNAs (lncRNAs) can affect tumorigenesis. Data from The Cancer Genome Atlas (TCAG) suggest that LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target. We conducted this study to verify this.Patients and Methods: We conducted this study to verify the opinion that “LINC02783 is highly expressed in renal cell carcinoma (RCC) and is expected to be a potential biological target”. We employed quantitative real-time polymerase chain reaction (qRT-PCR) to test LINC02783 expression in RCC tissues, CKK-8 assay and transwell assay to assess the viability and invasion of RCC cells, Western blot to quantify Sox-4 expression, dual-luciferase reporter (DLR) assay and RNA immunoprecipitation (RIP) assay to analyze the interaction between LINC02783 and miR-20b, in vivo experiments to test tumor formation.Results: We detected high LINC02783 expression in RCC patients. Patients with higher LINC02783 levels had a markedly poorer prognosis. In vitro and in vivo, the down-regulation of LINC02783 suppressed the viability and invasion of RCC cells. The DLR assay results revealed that LINC02783 enhanced Sox-4 expression by regulating miR-20b. LINC02783 can act as a sponge for miR-20b to inhibit Sox-4 expression.Conclusion: LINC02783 is highly expressed in RCC patients and indicates a poor prognosis. LINC02783 can affect the occurrence and progression of RCC through the miR-20b/Sox-4 axis, making it a promising target for the treatment of RCC.Keywords: LINC02783, miR-20b, Sox-4, renal cell carcinoma, The Cancer Genome Atlas, prognosis

Objective To investigate the association of serum leptin level with erectile dysfunction (ED).Methods A total of 38 out-patients complaining ED were graded by using IIEF-5 questionnaire.The mean IIEF-5 score of the patients was (12.3±2.4)and no one was higher than 21.The patients were divided into 3 groups according to their scores.The fasting serum leptin levels were determined by ELISA in 38 patients (patient group) and 15 healthy controls (control group).Their body weights and heights were measured for body mass index(BMI).Results Serum leptin levels were significantly correlated with BMI in both groups,regardless of patient ages and history.The mean leptin level in patients was (10.16±8.67) μg/L,which was significantly higher than that in the healthy controls ([4.81±3.66] μg/L,P0.05) after adjusting the effect of BMI.The leptin levels in the mild,moderate,and severe ED groups were (6.98±4.99) μg/L,(10.3±9.51) μg/L and (13.1±10.02) μg/L,respectively,with that of the mild group being significantly lower than those of the latter two groups(P0.05).Conclusion It is indicated that human serum leptin level may have a potential value in diagnosing ED.

In this paper, infant formula based on market research, due to different levels of consumer demand and consumption of infant formula relationship between the predictions for the improvement of market forecasting to provide a new method. We can find product attributes determine that the consumer buying behavior, degree of consumer acceptance of products by first-level product attributes directly affect the core products, paper products while the customer acceptance of correlation analysis of the reasons for doing. We find that the third-level consumer product attribute is becoming increasingly important to business should be doing the actual investigation for their products in the market set a good position to correct the first time to guide the consumer to buy or duplicate spending.

The TCP flow fairness problem in 802.11 based multihop ad hoc networks was studied in this paper. Firstly, a probability model was established to describe the TCP flow fairness, and its rationality was verified by simulation; Then, a new MAC layer scheme with low overhead cost was proposed, in which the backoff window at MAC layer was controlled by detecting the basic factors that lead to the TCP flow unfairness, and so the TCP flow fairness was improved. The proposed algorithm was simply and easy to implement, simulation results show that it can eliminate the TCP flow monopoly phenomenon, improve the TCP flow fairness validly and without significantly impairing aggregate throughput in multihop ad hoc networks.