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424 results on '"Galera M"'

15. Autologous bone marrow mononuclear cells in the treatment of chronic limb-threatening ischaemia: a proof-of-concept trial

Author

Chacon Quevedo, A, primary, Garcia-Revillo Garcia, J, additional, Sagrario Lombardo Galera, M, additional, Canis Lopez, M, additional, Munoz Carvajal, I, additional, Martin-Palanco, V, additional, De Alcala Martinez Gomez, D, additional, Garcia Rospide, V, additional, Patricio Linares Palomino, J, additional, Capel Aleman, A, additional, Castilla Llorente, C, additional, Perez Vallecillos, P, additional, Angeles Moreno Carrillo, M, additional, Tadeo Gomez Ruiz, F, additional, and Herrera Arroyo, C, additional

Published
2022
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24. Urinary exosomal miR-146a as a marker of albuminuria, activity changes and disease flares in lupus nephritis

Author

Perez-Hernandez J, Martinez-Arroyo O, Ortega A, Galera M, Solis-Salguero M, Chaves F, Redon J, Forner M, and Cortes R

Subjects
skin and connective tissue diseases
Abstract

BACKGROUND: Urinary exosomes, especially microRNAs (miRNAs) packaged within, are ideal sources of renal damage markers. We investigated the association between exosomal miR-146a, (anti-inflammatory regulator) and disease activity, proteinuria and systemic lupus erythematosus (SLE) flares over a 36-month follow-up period.; METHODS: We isolated urinary exosomes from 41 SLE patients, 27 with lupus nephritis (LN) and 20 healthy controls, and exosomal miR-146a, quantified by the real-time quantitative polymerase chain reaction (RT-qPCR), was correlated with histological features in 13 renal biopsies. We also analysed the association between the exosomal miR-146a and TRAF6 axis.; RESULTS: Exosomal miR-146a showed an inverse association with circulating C3 and C4 complement components, proteinuria, and with histological features such as chronicity index. This marker was able to identify LN with an AUC of 0.82 (p=0.001). Basal exosomal miR-146a was associated with disease activity and proteinuria changes and was an independent marker of 36-month follow-up flares (OR 7.08, p=0.02). Pathway analysis identified IRAK1 and TRAF6 as miR-146a target genes. Finally, in vitro experiments suggested that miR-146a exerts a protective effect through negative regulation of inflammation by suppressing IRAK1 and TRAF6.; CONCLUSIONS: Urinary exosomal miR-146a levels are correlated with lupus activity, proteinuria and histological features, discriminating patients with LN and being a good baseline marker of SLE flares. We have identified a relevant biological miR-146a-TRAF6 axis association in LN renal fibrosis progression.

Published
2021

25. Solving matrix effects exploiting the second-order advantage in the resolution and determination of eight tetracycline antibiotics in effluent wastewater by modelling liquid chromatography data with multivariate curve resolution-alternating least squares and unfolded-partial least squares followed by residual bilinearization algorithms: II. Prediction and figures of merit

Author

García, M.D. Gil, Culzoni, M.J., De Zan, M.M., Valverde, R. Santiago, Galera, M. Martínez, and Goicoechea, H.C.

Published
2008
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26. Solving matrix-effects exploiting the second order advantage in the resolution and determination of eight tetracycline antibiotics in effluent wastewater by modelling liquid chromatography data with multivariate curve resolution-alternating least squares and unfolded-partial least squares followed by residual bilinearization algorithms: I. Effect of signal pre-treatment

Author

De Zan, M.M., Gil García, M.D., Culzoni, M.J., Siano, R.G., Goicoechea, H.C., and Martínez Galera, M.

Published
2008
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33. Decreased Urinary Levels of SIRT1 as Non-Invasive Biomarker of Early Renal Damage in Hypertension

Author

Martinez-Arroyo O, Ortega A, Galera M, Solaz E, Martinez-Hervas S, Redon J, and Cortes R

Abstract

Sirtuins have become important players in renal damage in hypertension and diabetes, but their value as biomarkers is poorly assessed. The aims of the study were to evaluate the levels of sirtuin1 (SIRT1), and two miRNAs that regulate SIRT1 expression in hypertensive patients with incipient renal damage with and without diabetes. We quantified urinary SIRT1 and claudin 1 (CLDN1) mRNA and miR34-a and miR-200a levels by quantitative real-time polymerase chain reaction (RT-qPCR) from patients and in cultured podocytes treated with high glucose and angiotensin II. Western blot and fluorescence analyses were also performed. We found decreased SIRT1 levels in patients with increased urinary albumin excretion (UAE), the lowest with diabetes presence, and a strong association with UAE, discriminating incipient renal damage. In vitro experiments also showed SIRT1 overall decreases in podocyte cultures under treatment conditions. In urine samples, miR-34a was reduced and miR-200a increased, both related to UAE levels. However, both miRNAs were generally increased in podocyte cultures under high glucose and angiotensin-II treatment. These results show a significant urinary SIRT1 decrease in albuminuric hypertensive patients, strongly associated with albuminuria, suggesting that SIRT1 could be a potential and non-invasive method to assess incipient renal damage in hypertensive patients.

Published
2020

48. Use of the Cross-Section Technique Linked with Multivariate Calibration Methods To Resolve Complex Pesticide Mixtures

Author

Frenich, A. Garrido, Vidal, J.L. Martinez, and Galera, M. Martinez

Subjects
Pesticides -- Analysis, Multivariate analysis, Chemistry, Analytic -- Methods, Spectrum analysis -- Research, Chemistry
Abstract

The potential of the cross-section (CS) approach in combination with the partial least squares (PLS) and principal component regression (PCR) was assessed in the resolution of a complex pesticide mixture showing twelve overlapped components in High Performance Liquid Chromatography with Diode Army Detection (HPLCDAD). Careful selection of the CS through the three-dimensional (3D) (A, (lambda), t) data matrix gave two-dimensional (2D) signals with the best sensitivity for the determination of each pesticide. In all cases, the application of the PLS method demonstrated a better quantitative prediction ability than that of the PCR method. The CSPLS approach is a powerful analytical tool. Ten pesticides were well-resolved, while for the other two pesticides of the mix, re prediction ability was poor, and they could not be determined, probably due to their low net analytical signal. The CS-PLS model was evaluated by predicting the concentrations of independent test set samples. Finally, the proposed model was successfully applied for the determination of these pesticides in groundwater.

Published
1999

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