1. Interleukin-6 stimulates HHV-8 replication in bone marrow cultures and infected cell lines
- Author
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Félix Agbalika, Marolleau Jp, and Brouet Jc
- Subjects
DNA Replication ,Stromal cell ,viruses ,Bone Marrow Cells ,Virus Replication ,Polymerase Chain Reaction ,Cell Line ,Open Reading Frames ,Immune system ,Reference Values ,medicine ,Humans ,Interleukin 6 ,Multiple myeloma ,biology ,Lymphokine ,virus diseases ,Hematology ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Molecular biology ,medicine.anatomical_structure ,Real-time polymerase chain reaction ,Cell culture ,DNA, Viral ,Herpesvirus 8, Human ,biology.protein ,Bone marrow ,Stromal Cells - Abstract
INTRODUCTION The significance of HHV-8 DNA detection in bone marrow stromal cells from patients with multiple myeloma is still controversial. Since IL-6 plays a key role in the pathogenesis of myeloma, we studied the effect of this lymphokine on HHV-8 DNA detection. MATERIALS AND METHODS Amplification of HHV-8 DNA from long-term bone marrow cultures established from normal individuals in the presence or absence of 1 ng/ml IL-6 and from an HHV-8 infected ISI cell line. RESULTS AND CONCLUSIONS IL-6 increased HHV-8 replication in seven of ten bone marrow cultures as well as in the ISI cell line. Quantitative PCR showed a 3-100-fold increase in HHV-8 DNA copy number/microg DNA. These data suggest that when IL-6 is present in the micro-environment, HHV-8 replicates and may be amplified in the absence of systemic infection in patients without cellular immune deficiency.
- Published
- 2000