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Due to increasing antibiotic resistance, there is an urgent need to find new antibiotic alternatives or supporters for the treatment of disease-causing pathogens. For this reason the aim of the study was examine the antimicrobial and antifungal activity of Anatolian (Anadolu) honey bee venom (HBV) against Gram-positive and Gram-negative bacteria and yeast-like fungi. At first step chemical analyses of HBV was performed by HPLC method. According to the results of HPLC analysis, we obtained a good separation of apamine, phospholipase A2 and melittin with the ratio of 1.83%, 20.60% and 57.62% respectively. The antimicrobial and antifungal activity of the Anatolian HBV was tested against 9 Gram (+), 7 Gram (-), 1 acid-alcohol-resistant and 3 yeast fungi. First, the activity of the Anatolian HBV sample against these microorganisms was determined by the agar well diffusion method, then their zones were measured. The microdilution method was used to determine the minimum inhibitory concentration (MIC) for the antimicrobial activity tests. The results of MIC values were varied from 3.06 μg/mL to 50 μg/mL for the tested microorganisms. It was found that Mycobacterium smegmatis and Streptococcus pyogenes were the most susceptible bacteria (3.06 μg/mL), followed by Vibrio sp., Aeromonas sobria, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus (MRSA) and B. subtilis with a MIC concentration of 6.125 μg/mL. These findings strongly suggest that Anatolian HBV will be developed as a new antibacterial-antifungal drug against Gram-positive, Gram negative and antibiotic-resistant bacteria and yeast-like fungi. However, further research is required to evaluate their in vivo efficacy and safe and effective delivery methods for their therapeutic use.

Nosemosis, one of the most important honeybee diseases, causes colony losses and low productivity worldwide. A national study were carried out to presence of nosemosis throughout Türkiye, determine the prevalence of Nosema cereane and Nosema apis, and perform the network analyses of the parasites. Specimens were collected from hives with no clinical signs. A total of 1194 European honey bee colonies in 400 apiaries from 40 provinces of Türkiye were examined by microscopic and molecular techniques. Nosemosis were found in all of 40 provinces. The prevalence ratio were 64.3 ± 3,0, 95%CI in apiaries and 40.5 ± 2,9, 95%CI in hives. N. ceranae DNA were detected all of positive hives, while N. ceranae and N. apis co-infection in only four colonies. In the last two decades, it has been observed that nozemosis has spread to all provinces and its prevalence has increased in all regions of Türkiye. All of the N. ceranae or N. apis samples examined were 100% identical within themselves. Network analysis showed that they were within largest haplotype reported worldwide.

The use of phage and phage-based products for the prevention and treatment of bee disease is one of the promising natural alternatives to chemical or antibiotic treatments in beekeeping. A novel lysogenic bacteriophage, phage Pd22F (vB_PlaM_Pd22F), was isolated from Paenibacillus dendritiformis by the prophage induction method. This phage, which is capable of infecting Paenibacillus larvae and P. dendritiformis strains, was characterized by microbiological and comparative genomic analysis. Transmission electron microscopy images showed that phage Pd22F had the morphology of a myovirus. Whole-genome sequencing results showed that vB_Pla M_Pd22F has an 86,388-bp linear dsDNA genome with a GC content of 50.68%. This genome has 124 coding sequences (CDSs), 53% of which encode functionally unknown proteins and 57 of which encode proteins that show similarity to known proteins. In addition, one tRNA gene was found. The phage Pd22F genome does not contain any antimicrobial resistance genes. The similarity between the genome sequence of phage Pd22F and the whole genome sequences of other Paenibacillus phages available in the NCBI Virus Database was found to be below 50% (42%), indicating that phage Pd22F differs greatly from previously characterized phages at the DNA level. The results of comparative genomics and phylogenetic analysis revealed that Pd22F is a new phage belonging to the family Myoviridae, order Caudovirales. This is the first report of genomic and morphological characterization of a Paenibacillus dendritiformis prophage.

Acarapis woodi (Rennie, 1921) (Acari: Tarsonemidae) is one of the mites that settles in the respiratory system of honeybees (Apis mellifera L. (Hymenoptera, Apidae)) and distributed throughout the world. It causes significant economic losses on honey production. In Türkiye, studies on the existence of A. woodi are very limited and so far, no studies on the molecular diagnosis and phylogenetic of it have been reported in Türkiye. This study was conducted to investigate the prevalence of A. woodi in Türkiye, especially in areas where beekeeping is intense. Diagnosis of A. woodi was performed using both microscopic and molecular methods using specific PCR primers. Adult honeybee samples were collected from 1.193 hives in 40 provinces of Türkiye between 2018 and 2019. Based on identification studies, the presence of A. woodi was detected in a total of 3 hives (0.5%) in 2018 and 4 hives (0.7%) in 2019. This is the first report for determination of A. woodi in Türkiye.

This study aims to identify lactic acid bacteria (LAB) isolated from honeybees (Apis mellifera workers and larvae) in detail and to determine their functional probiotic properties. A total of 11 strains were classified based on morphological and biochemical characteristics. Preliminary probiotic properties of strains, that were molecularly identified using 16 S rRNA, such as antimicrobial activity, tolerance to digestive conditions, aggregation ability, were investigated. The antimicrobial properties of strains were tested against a wide range of human pathogens. All strains that showed γ-hemolysis and did not contain bacteriophages were considered safe. The strains' survivability checked for 0.3% bile and 3.0-7.8 pH contents was promising. The highest autoaggregation ranged from 14.7 to 30.76% after 4 h. Tested LAB strains markedly exhibited coaggregation with Listeria monocytogenes and Escherichia coli. According to the results, tested bacteria showed significant antagonistic effects against pathogens, and positive probiotic characteristics compatible with in vitro gastrointestinal tract conditions. The results suggest that Apis mellifera LAB symbionts may have a probiotic potential, and be effective and safe candidates for human use. This study provides an addition to the development of the current knowledge by defining in detail honeybee-associated bacteria and determining their probiotic potential.

The aim of this study is to determine the effect of growing media on antioxidant properties and antimicrobial activities of Pleurotus ostreatus(Jacq. ex Fr.) P. Kumm. In the study, growing substrates were prepared with five tree sawdust (80%) and waste (20% Tea Waste or Wheat Bran) at different ratios (80% Sawdust + 20% TW, 80% Sawdust + 20% GW). The antimicrobial activities of methanol extracts from 19 P. ostreatus against microorganisms were tested by agar well diffusion technique. The total phenolic content was measured using the Folin-Ciocalteau procedure. The antioxidant capacity was determined using ferric reducing antioxidant power (FRAP) and free radical scavenging activity of DPPH. It was observed that the addition of 20% tea waste in the substrate had a positive effect on antimicrobial activity. It was determined that the methanol extract of mushrooms obtained from the 80QS+20TW substrate was the most effective extract against to all of microorganisms investigated in this study. Significant differences (P, Öz: Bu çalışmanın amacı, yetiştirme ortamının Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm.'un antioksidan özellikleri ve antimikrobiyal aktiviteleri üzerindeki etkisini belirlemektir. Çalışmada beş adet talaş (%80) ve atık (%20 Çay Atığı veya Buğday Kepeği) ile farklı oranlarda (%80 Talaş + %20 TW, %80 Talaş + 20% GW) yetiştirme substratları hazırlanmıştır. Toplam 19 farklı ortamda yetiştirilen P. ostreatus'tan elde edilen metanol ekstraktların, mikroorganizmalara karşı antimikrobiyal aktiviteleri agar kuyu difüzyon tekniği ile test edildi. Toplam fenolik içerik, Folin-Ciocalteau prosedürü kullanılarak ölçülmüştür. Antioksidan kapasitesi, DPPH'nin ferrik indirgeyici antioksidan gücü (FRAP) ve serbest radikal süpürücü aktivitesi kullanılarak belirlendi.. 80QS+20TW substratından elde edilen mantarların metanol ekstraktının bu çalışmada incelenen tüm mikrooganizmalara karşı en etkili ekstrakt olduğu belirlendi. Farklı substratlar üzerinde yetiştirilen mantar ekstraktlarının antioksidan içerikleri arasında önemli farklılıklar (P

In the gastrointestinal tract of honey bees (Apis mellifera), there is a unique collection of microorganisms, called microflora or microbiota, which have various mgeyietabolic activities in the bee crops, which can protect against various microbial threats, and is linked to powerful mechanisms of action in the bee immune system. The adult honey bees especially have a rich microflora. Their microbiome is considered to be one of the most important and remarkable factors in maintaining bee health. Like humans, honey bees (Apis mellifera) are social creatures and work cooperatively to ensure the continuity of life in the hive. In productive colonies, the increasing diversity of microbiota depends on various factors, from the developmental age of the bee to its feeding, from the geographical location of the hive to the local climate. The lack of diversity of the bacterial community may negatively affect the productivity of the bee hive. This review describes the diversity of the honey bee intestinal core microflora, which are of great importance for bee life, the ways in which they acquire the bacteria, how they colonize in the gastrointestinal tract of bee and their associated beneficial mechanisms for honey bee survival.

Çalışmada çay toprağından izole edilen Trichoderma harzianum ID11C suşunun bazı enzim aktivitesi, ağır metallere toleransı, tohum (domates, mısır ve fasulye) çimlenmesi üzerine etkinliği ve fasulyede Rhizoctonia solani B227 (AG-4)'nin biyotik stresine karşı biyokontrol aktivitesinin araştırılması amaçlandı. T. harzianum ID11C suşunun bazı enzim aktiviteleri kromojenik yöntemle incelendi. Sonuçlar farklı konsantrasyonlarda ağır metal (Cu, Pb, Zn ve Cd) ve tuz toleransı olduğunu gösterdi.T. harzianum ID11C Rhizoctonia solani B227(AG4) izolatının fasulye gelişimine karşın patojenite testi ve T. harzianum ID11C suşunun tohum çimlenme başarısına olan etkinliği, su agar metodu ile incelendi. T. harzianum ID11C ve R. solani B227 varlığında fasulye gelişim parametreleri saksı deneyi ile araştırıldı. Fizyolojik (ana kök ve gövde uzunluğu, saçaklanma sayısı, yaprak sayısı, yaş ve kuru ağırlıkları) parametreler ölçüldü ve istatistiksel analizleri yapıldı.Trichoderma harzianum ID11C suşu, yüksek konsantrasyonlarda ağır metallere (100-400 mM) ve tuza (250 mM) karşı toleranslıdır. Bitki gelişimini teşvik eden enzim aktivitelerinin var olduğu gözlendi. T. harzianum suşunun tohumların çimlenme başarısı üzerinde kontrole kıyasla olumsuz bir etkisinin olmadığı tespit edildi. Saksı deneyinde, kontrol ile R. solani B227 grupları birbirine kıyaslandığında, gövde ve ana kök uzunluğu, saçaklanma sayısı ve kökte lezyon skala değerleri ile yapılan istatistik analizi sonucu aralarında anlamlı farkın (p< 0.05, Tukey) olduğu belirlendi. R. solani B227 ve T. harzianum ID11D’nin ayrı ayrı fasulye gelişimi üzerine olan etkileri, kontrol grubuna göre iyi olmadığı belirlendi. Ancak ID11C ve B227+ID11C grupları B227 grubu ile kıyaslandığında, patojenik etkinliği azalttığı belirlendi. R. solani B227 (AG4) izolatı, fasulyede (Zulbiye) patojeniteye neden olduğu, T. harzianum ID11C suşunun ise istatistiksel olarak olumlu yönde anlamlı bir fark oluşturmamasına rağmen, tüm parametrelerde patojeniteyi azalttığı belirlendi. T. harzianum ID11C suşunun fasulye tarımında Rhizoctonia solani’ye karşı biyokontrol ve bitki destekleyici ajan olarak kullanılabileceği sonucuna varıldı.

Paenibacillus larvae (P. larvae) is a pathogenic bacterium causing American Foulbrood Disease (AFB) in honeybee larvae. It is necessary to develop alternative strategies for the control of this disease due to the serious honeybee colonies loses and the use of antibiotics. Recent studies are aimed at the investigating natural fighting agents against P. larvae. In our study, it was aimed to demonstrate potential antibacterial efficacy of ethanol extract of Anatolian Propolis (EAP) against P. larvae strains PB35 and SV35 which were isolated in Turkey. The total phenolic content (TPC) and flavonoid content (TFC) of EEAP were determined as 181.73±5.20 mg Gallic Acid Equivalents (GAE)/g, and 42.33±1.40 mg Quercetin Equivalents (QE)/g, respectively. It was found that EAP contains different amounts of ferulic, caffeic, coumaric acids, rutin, and caffeic acid phenethyl ester (CAPE). The antibacterial activity of the EAP was determined by using agar-well diffusion, microdilution, and Bioscreen C techniques. The Minimal Inhibition Concentration (MIC) values of the EAP were determined as 74.87 μg/ml against strain PB35 and SV35 using both microdilution and Bioscreen C teqnique. In both techniques, Minimal Bactericidal Concentration (MBC) values of the EAP were evaluated as 149 and 598.4 μg/ml against strain PB35 and SV35, respectively. The fact that EAP shows low concentrations of bacteriostatic (MIC) and bactericide (MBC) activity values against P. larvae strains, spore-forming bacilli, which are agents of AFB disease, suggests that it may be a potential source in AFB disease control. Paenibacillus larvae (P. larvae), bal arısı larvalarında Amerikan Yavru Çürüklüğü hastalığına (AYÇ) neden olan patojenik bir bakteridir. P. Larvae tedavisinde kullanılan antibiyotikler bal arısı kolonilerinin ciddi kaybına neden olmakta ve bu nedenle antibiyotik kullanımına bağlı olarak bu hastalığın kontrolü için alternatif stratejiler geliştirmek gerekmektedir. Son çalışmalar, P. larvae’ya karşı doğal mücadele ajanlarını araştırmaya yöneliktir. Çalışmamızda Anadolu propolisi etanolik ekstresinin (EAP) Türkiye'de izole edilen P. larvae suşları PB35 ve SV35'e karşı potansiyel antimikrobiyal etkinliğinin ortaya konması amaçlanmıştır. EAP, toplam fenolik madde (TPC), flavonoid madde miktarları (TFC) ve bazı fenolik bileşikler açısından karakterize edilmiştir. Analizlere göre, EAP'nin total fenolik madde miktarı ve flavonoid madde miktarları sırasıyla 181,73±5,20 mg Gallik Asit Eşdeğeri (GAE)/g TFC olarak 42,33±1,40 mg Kuersetin Eşdeğeri (QE)/g ve farklı miktarlarda ferulik, kafeik, kumarik asitler, rutin ve kafeik asit fenetil ester (CAPE) içerdiği bulundu. EAP'nin antimikrobiyal aktivitesi, agar kuyu difüzyon, mikrodilüsyon ve Bioscreen C teknikleri kullanılarak belirlendi. EAP'nin Minimal İnhibisyon Konsantrasyon (MIC) değerleri, hem mikrodilüsyon hem de Bioscreen C tekniği kullanılarak PB35 ve SV35 suşuna karşı 74,87 μg/ml olarak belirlendi. Her iki teknikte de PB35 ve SV35 suşlarına karşı EAP'nin minimal bakterisidal konsantrasyon (MBC) değerleri sırasıyla 149 ve 598,4 μg/ml olarak belirlendi.

The goal of the work is to determine the bioactive pharmaceutical metabolites produced by the Fusarium oxysporum YP9B isolate. Ten new natural compounds were isolated from the ethyl acetate extract of the F. oxysporum YP9B strain. Their structures were elucidated by spectroscopic methods using 1D and 2D NMR, UV, FT-IR, and mass spectra (LC-QTOF MS and GC-FID/MS). Identified compounds were named as; (1-benzyl-2-methoxy-2-oxoethyl)-2-hydroxy-3-methylbutanoate (1), 2-oxo-8-azatricyclo[9.3.1.13,7]-hexadeca-1(15),3(16),4,6,11,13-hexaen-10-one (2), 2,3-dihydroxypropanoic, hexadecanoic anhydride (3a), 2,3-dihydroxypropanoic (9Z)-octadecenoic anhydride (3b), 2,3-dihydroxy-propanoic (9Z,12Z)-octadecadienoic anhydride (3c), 2,3-dihydroxypropanoic (11Z)-octadecenoic anhydride (4a), 2,3-dihydroxypropanoic, (9E,12E)-octadecadienoic anhydride (4b), 3-hydroxy-1,2,6,10-tetramethylundecyl hexzadecanoate (5a), 3-hydroxy-1,2,6,10-tetramethylundecyl (9E)-octadecaenoate (5b), and 3-hydroxy-1,2,6,10-tetramethylundecyl octadecanoate (5c). Antimicrobial activities of the isolates obtained from the YP9B strain were determined. Cytotoxic and antiviral activities were tested for the isolates against VERO, MCF-7, PC-3, and A549. Compounds 5a-c, 1, and 3a-c showed bacteriostatic activity at low concentrations, and 4a-b and 2 were found to be bactericides. MIC and MBC values against Mycobacterium smegmatis for the compounds 5a-c and 1 were determined to be ​​