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Soil aggregates and associated carbon (C) and nitrogen (N) stocks are important for soil organic matter (SOM) consequences and quality. The study is meant to understand the mechanisms of how fertilization management affects Cand Nstocks. Tobacco monocropping systems with four fertilizer treatments (0, 75, and 112kg Nha−1, and 60kg Nha−1 + manure) were established in 1998. After 19 years, crop monocrop and fertilization management redetermined soil aggregates and related indicators. The results showed the large aggregates proportion (LMP) and small macroaggregates proportion (SMP) varied inversely with changing season. The LMP gradually rose after the implementation of tillage while microaggregates gradually declined. By manure fertilizer, LMP and SMP were significantly higher than those in inorganic fertilizer treatments, while microaggregates proportion (MIP) and silt and clay proportion (SCP) were lower than in inorganic fertilizer treatments. Soil water-stable aggregate and their associated soil organic stocks are changed with growing season, and tillage practices would have potentially affected these indicators, while the proportion and soil organic stocks more quickly recovered in large aggregates by manure application. Therefore, the proper application of manure combined with chemical fertilizer would maintain soil quality in tobacco cultivation. [ABSTRACT FROM AUTHOR]

Despite many studies on the influence of cropping practices on soil microbial community structure, little is known about ecological patterns of rare and abundant microbial communities in response to different tobacco cropping systems. Here, using the high-throughput sequencing technique, we investigated the impacts of two different cropping systems on soil biochemical properties and the microbial community composition of abundant and rare taxa and its driving factors in continuous and rotational tobacco cropping systems in the mountain lands of Yunnan, China. Our results showed that distinct co-occurrence patterns and driving forces for abundant and rare taxa across the different cropping systems. The abundant taxa were mainly constrained by stochastic processes in both cropping systems. In contrast, rare taxa in continuous cropping fields were mainly influenced by environmental perturbation (cropping practice), while governed by deterministic processes under rotational cropping. The α-diversity indices of rare taxa tended to be higher than those of the abundant ones in the two cropping systems. Furthermore, the network topologies of rare taxa were more complex than those of the abundant taxa in the two cropping systems. These results highlight that rare taxa rather than abundant ones play important roles in maintaining ecosystem diversity and sustaining the stability of ecosystem functions, especially in continuous cropping systems. [ABSTRACT FROM AUTHOR]

Nitrogen fertilizer is essential for the yield and quality of flue-cured tobacco. Honghuadajinyuan (i.e. Hongda) is a special flue-cured tobacco variety in Yunnan, which differs in nitrogen application rates from other varieties. In 2016 and 2017, different nitrogen application rates were established in Yunnan to compare, with other varieties, Hongda's agronomic, economic, chemical indicators and soil residual nitrogen. As nitrogen fertilizer rate increased, the ratio of fresh weight to dry weight (RFD) and the proportions of inferior tobacco (PIT) initially decreased and then increased in contrast to the proportions of superior tobacco (PST) and average price. Total sugar and reducing sugar contents in flue-cured tobacco leaves decreased as nitrogen fertilizer rate increased, while total nitrogen, nicotine, and protein contents increased. The highest sensory evaluation scores were obtained with 45 and 60 kg N ha−1. Soil nitrate contents increased with increasing nitrogen fertilizer rate and significantly increased when nitrogen application rate exceeded 60 kg N ha−1. A quadratic model was suitable for Hongda's response to nitrogen fertilizer rate and 60 kg N ha−1 can meet the needs of the growth of this flue-cured tobacco variety. Abbreviations: Hongda: Honghuadajinyuan; RFD: ratio of fresh to dry weight; PIT: proportion of inferior tobacco; PMT: proportion of medium tobacco; PST: proportion of superior tobacco; SPAD: soil and plant analyzer development. [ABSTRACT FROM AUTHOR]

Background: The leaf age for harvesting flue-cured tobacco leaves is closely related to the quality of tobacco leaves, so an appropriate leaf age for harvesting is important for improving yield and quality of flue-cured tobacco, however, at present, there are few studies on effects of leaf age on physiological and biochemical changes during flue-curing and there is no clear standard of proper leaf ages for harvesting in production. Results: In the Yunnan tobacco-growing area, an experiment was carried from 2016 to 2017 and different leaf ages were set. The results demonstrate that leaf age has a significant on tissue cell gap, leaf age and flue-curing stages exert significant effects on upper epidermis, palisade and spongy tissue, and leaf thickness of tobacco leaves. The thicknesses of upper and lower epidermis as well as palisade and spongy tissues at different ages show an approximately W-shaped change trend during flue-curing. With the advance of flue-curing stages, contents of starch, chlorophyll, carotenoid, and water in tobacco leaves at different leaf ages decrease, while polyphenol and malondialdehyde (MDA) contents increase. The older the leaf, the faster the chlorophyll, carotenoid, and water contents reduce, while the faster the polyphenol and MDA content rise during flue-curing. The flue-cured tobacco leaves at 116 DAT (days after transplanting) show the highest contents of total nitrogen and nicotine, followed by 123 DAT and those at 130 DAT are the lowest; however, the contents of total sugar and reducing sugar demonstrate a contrary tendency, and the starch content at 116 DAT is much lower than those in the other two treatments. The proportion of superior tobacco, average price, yield, and output value of upper tobacco leaves at different leaf ages are the highest at 123 DAT. The highest sensory evaluation score is found at 123 DAT, while that at 130 DAT is significantly lower in comparison with the other two treatments. Conclusions: Tobacco leaves harvested at 123 DAT are mature and exhibit a low degree of membrane lipid peroxidation, moderate chemical compositions, and high economic value. 123 DAT improves availability of tobacco leaves. [ABSTRACT FROM AUTHOR]