1. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates.
- Author
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Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Berenji J, and Krstić B
- Subjects
- Alfalfa mosaic virus genetics, Alfalfa mosaic virus immunology, Alfalfa mosaic virus isolation & purification, Cucumovirus genetics, Cucumovirus immunology, Cucumovirus isolation & purification, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Plant Viruses genetics, Plant Viruses immunology, Potyvirus genetics, Potyvirus immunology, Potyvirus isolation & purification, Reverse Transcription, Sequence Analysis, DNA, Serbia, Tobacco Mosaic Virus genetics, Tobacco Mosaic Virus immunology, Tobacco Mosaic Virus isolation & purification, Tospovirus isolation & purification, Yugoslavia, Phylogeny, Plant Diseases virology, Plant Viruses isolation & purification, Nicotiana virology, Tospovirus classification, Tospovirus genetics
- Abstract
In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY. A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.
- Published
- 2011
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