1. Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak.
- Author
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Tsuyoshi Sekizuka, Kentaro Itokawa, Tsutomu Kageyama, Shinji Saito, Ikuyo Takayama, Hideki Asanuma, Naganori Nao, Rina Tanaka, Masanori Hashino, Takuri Takahashi, Hajime Kamiya, Takuya Yamagishi, Kensaku Kakimoto, Motoi Suzuki, Hideki Hasegawa, Takaji Wakita, and Makoto Kuroda
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SARS-CoV-2 , *COVID-19 , *CRUISE ships , *DIAMONDS , *PRINCESSES - Abstract
The Diamond Princess cruise ship was put under quarantine offshore Yokohama, Japan, after a passenger who disembarked in Hong Kong was confirmed as a coronavirus disease 2019 case. We performed whole-genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from PCR+ clinical specimens and conducted a phylogenetic analysis of the outbreak. All tested isolates exhibited a transversion at G11083T, suggesting that SARS-CoV-2 dissemination on the Diamond Princess originated from a single introduction event before the quarantine started. Although further spreading might have been prevented by quarantine, some progeny clusters could be linked to transmission through mass-gathering events in the recreational areas and direct transmission among passengers who shared cabins during the quarantine. This study demonstrates the usefulness of haplotype network/phylogeny analysis in identifying potential infection routes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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