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• Iron bound P release dominated during algal regime. • P-solubilizing bacteria contributed to P release during regime shift. • Coupling of C, N, P, O and Fe fueled P release. • Algal species composition differed during different regimes. In order to better understand the pathways and mechanisms of phosphorus (P) supply under different regimes, 12 sampling sites from 4 basins of 2 lakes were studied seasonally from October 2017 to July 2018 in Wuhan City, China. Concentrations of different forms of P and nitrogen (N) in surface and interstitial water, contents of carbon (C), N, P and iron (Fe) compounds as well as related extracellular enzymatic activities, phosphorus sorption, abundance of phosphorus-solubilizing bacteria (PSB), total and specific (containing phosphatase gene) microbial community composition in sediments were analyzed. In lakes with macrophyte dominance, P supply pathway from sediment to water column was blocked. In lakes being early period of regime shifting from macrophyte to algae, exogenous P input was the main P supply mode. In lakes being later period of regime shifting from macrophyte to algae, organic P hydrolysis and calcium-bound P dissociation driven by PSB contributed greatly to P regeneration, which was continuous and trickling. In this process, rapid C and N cycles fueled P regeneration. In lakes with algal dominance, given the significantly higher iron-bound P (Fe(OOH)~P), equilibriums phosphorus concentration and dehydrogenase activity, the main P regeneration pathway might be the desorption of Fe(OOH)~P driven by anoxia, showing the seasonal and pulsed characteristics. In addition, during the process of regime shift from macrophyte to algae, the dominant algal species switched from cyanobacteria to Chlorophyta. P-solubilizing microorganisms correlated with environmental factors, suggesting the coupling of multiple nutrient cycles, especially C, N, P, oxygen (O) and Fe, could effectively increase the pathways diversification and the strength of P regeneration. Image, graphical abstract [ABSTRACT FROM AUTHOR]

Dissimilatory nitrate reduction to ammonium (DNRA) plays an important role in controlling nitrogen (N) loading in lake ecosystems. However, studies on the linkage between DNRA bacterial community structure and lake eutrophication remain unclear. We examined the community and abundance of DNRA bacteria at six basins of four shallow lakes with different degrees of eutrophication in China. Measurements of the different forms of N and phosphorus (P) in the water column and interstitial water as well as total organic carbon (TOC) and sulfide in the sediments in summer (July 2016) were performed. The nutritional status of Lake Chaohu was more serious than that of the lakes in Wuhan, including Lake Qingling, Lake Houguan, and Lake Zhiyin by comparing geochemical and physical parameters. We found a higher abundance of the nrfA gene, which is a function gene of DNRA bacteria in sediments with higher contents of TOC and sulfide. Moreover, nitrate was a significant factor influencing the DNRA bacterial community structure. A significant difference of the DNRA bacterial community structure between Lake Chaohu and the lakes in Wuhan was discovered. Furthermore, DNRA bacterial abundance and community positively correlated with NH4+ and Chl a concentrations in Lake Chaohu, in which a percent abundance of dominant populations varied along eutrophication gradients. Overall, the abundance and community structure of the DNRA bacteria might be important regulators of eutrophication and cyanobacteria bloom in Lake Chaohu. [ABSTRACT FROM AUTHOR]