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Spodoptera frugiperda (Lepidoptera: Noctuidae) is a widely distributed pest of corn. Since it invaded China in 2018, it has caused serious damage to local corn production. Chlorantraniliprole, an anthranilic diamide insecticide, has been widely used to control lepidopteran pests. Tetrachloropyramid is a new allosteric modulator insecticide developed based on chlorantraniliprole, so it has a similar mechanism and insecticidal effect. In this study, we investigated resistance levels to chlorantraniliprole and tetrachloropyramid in S. frugiperda from 13 populations in China. Among the populations tested, the relative highest resistance to chlorantraniliprole occurred in the Guangzhou population, and the most susceptible to chlorantraniliprole was found in the Wuhan population. The lethal dosage LD50 value of the Guangzhou population against chlorantraniliprole was 27.8‐fold higher than that of the Wuhan population. Minimal differences were observed among S. frugiperda populations in terms of sensitivity to tetrachloropyramid. Heterozygous mutations at the I4734 site of the ryanodine receptor (RyR) were found, while no mutations were found in the G4891 site. The mutations were detected in only two of the 786 individuals analyzed, one from the Qinzhou population and other from the Anshun population (frequency below 2% in both cases). There were no significant differences in the expression levels of RyR between Guangzhou and Wuhan populations. In summary, our results indicate that: (i) S. frugiperda has low resistance levels to diamide insecticides in China; and (ii) the differences in relative resistance among the 13 populations analyzed are not caused by the mutations in RyR or the expression of RyR. [ABSTRACT FROM AUTHOR]

Abstract: In this paper, percentage of vegetation cover in different districts of Wuhan were calculated based on remote sensing images from 1988 to 2002 by employing NDVI method for dimidiate pixel model. Then the vegetation coverage maps in different periods were generated to analyze the temporal change of vegetation coverage of Wuhan. The results showed that, the average vegetation coverage of the whole area was decreased from 58.41% to 50.45%, especially in Jiangxia and urban district. From 1996 to 2002, the vegetation coverage decreased most sharply, while the vegetation coverage increased lightly from 1991 to 1996 in the whole study area. By analyzing spatial changing character in the whole study area we can see that, the whole area was in the decreasing moment, especially in Jiangxia district and the urban district, this was caused by the urban development, and may led to the malign development of the environment, worthy of all aspects of attention. [Copyright &y& Elsevier]