1. Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry
- Author
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Jefferies, Ryan, Morgan, Eric R., Shaw, Susan, and Heesom, Kate
- Subjects
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NEMATODES , *PARASITIC vaccines , *MASS spectrometry , *PROTEASE inhibitors , *METASTRONGYLIDAE , *ENZYME-linked immunosorbent assay , *NEMATODE infections , *IMMUNOGLOBULINS , *ELECTROPHORESIS , *WESTERN immunoblotting , *IMMUNOLOGY , *DIAGNOSIS - Abstract
Abstract: Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by cross-reactive recognition of antibodies to other endemic nematodes. This study compared the immuno-dominant soluble somatic proteins for first stage, third stage and adult A. vasorum using Western blotting. 2D electrophoresis and mass spectrometry were further used to identify specific immuno-dominant proteins (n =14) within the soluble fraction from whole adult worms. Proteins included an aspartyl protease inhibitor, representing a potential candidate for a recombinant-based ELISA and novel vaccine. [Copyright &y& Elsevier]
- Published
- 2011
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