1. Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus).
- Author
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Bolland SJ, Zahedi A, Oskam C, Murphy B, and Ryan U
- Subjects
- Actins chemistry, Actins genetics, Animals, Base Sequence, Biological Evolution, Cryptosporidiosis epidemiology, Cryptosporidium classification, Cryptosporidium genetics, Cryptosporidium ultrastructure, DNA, Protozoan chemistry, DNA, Protozoan isolation & purification, Fish Diseases epidemiology, Fisheries, Genotype, Likelihood Functions, Microscopy, Electron, Transmission veterinary, Phylogeny, Polymerase Chain Reaction veterinary, Prevalence, RNA, Ribosomal, 18S chemistry, Washington epidemiology, Western Australia epidemiology, Cichlids parasitology, Cryptosporidiosis parasitology, Cryptosporidium physiology, Fish Diseases parasitology
- Abstract
The species name Cryptosporidium bollandi n. sp. is proposed for Cryptosporidium piscine genotype 2 based on morphological, biological and molecular characterisation. Phylogenetic analyses of 18S rRNA (18S) sequences revealed that C. bollandi n. sp. was most closely related to piscine genotype 4 (5.1% genetic distance) and exhibited genetic distances of 10.0%, 12.2% and 25.2% from Cryptosporidium molnari, Cryptosporidium huwi and Cryptosporidium scophthtalmi, respectively. At the actin locus, C. bollandi n. sp. was again most closely related to piscine genotype 4 (6.8% genetic distance) and exhibited 15.5% (C. molnari), 18.4% (C. huwi), 22.9% (C. scophthalmi) and up to 27.5% genetic distance from other Cryptosporidium spp. (Cryptosporidium felis). Phylogenetic analysis of concatenated 18S and actin sequences showed that C. bollandi n. sp. exhibited 12.9% (C. molnari) to 21.1% (C. canis) genetic distance from all other Cryptosporidium spp. Genetic data as well as previous histological analysis clearly supports the validity of C. bollandi n. sp. as a separate species., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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