1. High qnrS retention of ESBL-producing and mcr-harbouring colistin-resistant Escherichia coli in Vietnamese food products.
- Author
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Nakayama T, Jinnai M, Miyaji K, Saito M, Ohata N, Yamaguchi T, Tran Nguyen Minh D, Nguyen Hoang O, LE Thi H, Ngo Thanh P, Hoang Hoai P, Nguyen DO P, Dang VAN C, Kumeda Y, and Hase A
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Chickens microbiology, Ciprofloxacin pharmacology, Food Microbiology, Microbial Sensitivity Tests, Plasmids genetics, Vietnam epidemiology, beta-Lactamases genetics, beta-Lactamases metabolism, Colistin pharmacology, Drug Resistance, Bacterial genetics, Escherichia coli genetics, Escherichia coli drug effects, Escherichia coli isolation & purification, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Meat microbiology
- Abstract
Plasmid-mediated antibiotic-resistant bacteria's transmission is fatal and a major threat to public health. This study aimed to clarify the presence of plasmid-mediated quinolone resistance（PMQR）genes in extended-spectrum β-lactamase（ESBL）-producing or/and mcr-harbouring colistin（COL）-resistant Escherichia coli（ESBL-COL-EC）isolates from Vietnamese and Japanese chicken meat. Resistance towards ciprofloxacin（CIP）was examined in 308 ESBL-COL-EC isolates; CIP-resistant ESBL-COL-EC isolates were examined for the PMQR gene. Approximately, 71.1% and 38.1% of ESBL-COL-EC and ESBLproducing E. coli isolates from Vietnamese and Japanese chicken meat were CIP-resistant, respectively. Multiplex PCR led PMQR detection showed that 35.2% of CIP-resistant ESBL-COL-EC isolates from Vietnamese food contained PMQR gene, whereas CIP-resistant ESBL-COL-EC isolates from Japanese chicken meat did not. Conjugation assays showed that the transmission of qnrS gene carried by E. coli to Salmonella. In conclusion, ESBL-COL-EC isolates from Vietnamese food are associated with a high frequency of fluoroquinolone resistance and a high distribution of the qnrS gene.
- Published
- 2024
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