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Soybean accounts for over a quarter of the world's oilseed consumption and over 70% of the world's protein meal consumption. The separate development of high oleic, low linolenic acid (HOLL) soybean and high‐protein (HP) soybean means that no soybean cultivar on the market has an optimal fatty acid profile and increased protein. The objective of this study was to develop and evaluate high protein, high oleic acid, and low linolenic acid (HP‐HOLL) soybean. A five‐gene stack was created using a two‐phase forward breeding scheme and marker‐assisted selection method. Forty‐six HP‐HOLL lines from three genetic backgrounds were grown in six environments in the Southeast United States. Although genotype‐by‐environment interaction was significant for seed composition traits, lines met the >75% and <3% cutoffs for oleic acid and linolenic acid, respectively, and met or exceeded the protein concentration of the HP parent. No negative interaction could be detected between the HP and HOLL traits. Additionally, yield testing in four environments indicated yield parity for some lines, suggesting HP and HOLL soybean cultivars with high yield could be selected. [ABSTRACT FROM AUTHOR]

Simple Summary: Research on nutritional requirements in combination with improving bee protection has recently increased because of the continuous global decline in the abundance and diversity of bees. The decline is currently attributed to several stressors, including climate change, diminishing forage resources, and pesticide use, among other factors. Industrial hemp (Cannabis sativa L.) is a new crop that is grown over a wide geographic area in the United States, providing economic and nutritional benefits to humans. However, the contribution of hemp floral resources (pollen) to bee nutrition is not well understood. We investigated the chemical composition of pollen from four industrial hemp varieties (Canda, CFX-2, Henola, and Joey) and documented the abundance and diversity of bees on the crop using two sampling methods. Results showed differences in composition among the four hemp varieties. Overall, the Joey variety was the most preferred by bees, despite expressing lower protein, amino acid, and saturated and monosaturated fatty acid content. Based on our findings, we concluded that industrial hemp pollen provides some nutritional benefits to bees. However, it is important to understand that multiple sources of pollen are needed for sustained bee survival. Apart from its economic value, industrial hemp (Cannabis sativa L.) is a prolific pollen producer, serving as a food source for bees. However, little is known regarding the extent to which varietal differences in hemp pollen chemistry influences bee preference. Here, we report the chemical profile of pollen from four hemp varieties (Canda, CFX-2, Henola, and Joey) and bee abundance and diversity, using direct visual counts and pan traps. The number and type of bees on each variety was recorded and the chemical composition (proximate analysis and mineral, amino, and fatty acid profiles) of pollen from each hemp variety was determined. During the entire sampling period, three bee types (bumble bees, honey bees, and sweat bees) were recorded, with a combined total of 1826. Among these, sweat bees and bumble bees were the most prevalent and were highest on the Joey variety. The four varieties expressed protein content ranging from 6.05% to 6.89% and the highest in Henola. Seventeen amino acids were expressed in all varieties, with leucine recording the highest content ranging from 4.00 mg/g in Canda to 4.54 mg/g in Henola. In general, Henola expressed high protein, amino acid, and saturated and monosaturated fatty acid contents and recorded significantly fewer bees compared with Joey, which had a low content of these components and a high content of polyunsaturated fatty acids. Our findings suggest that, while industrial hemp offers abundant and accessible pollen that would promote bee health and sustainability of their ecosystem services, the nutritional quality may not be adequate for bee growth and development as an exclusive pollen source. [ABSTRACT FROM AUTHOR]

Higher dietary protein, alone or in combination with physical activity (PA), may slow the loss of age-related muscle strength in older adults. We investigated the longitudinal relationship between protein intake and grip strength, and the interaction between protein intake and PA, using four longitudinal ageing cohorts. Individual participant data from 5584 older adults (52 % women; median: 75 years, IQR: 71·6, 79·0) followed for up to 8·5 years (mean: 4·9 years, SD: 2·3) from the Health ABC, NuAge, LASA and Newcastle 85+ cohorts were pooled. Baseline protein intake was assessed with food frequency questionnaires and 24-h recalls and categorized into < 0·8, 0·8–<1·0, 1·0–<1·2 and ≥ 1·2 g/kg adjusted body weight (aBW)/d. The prospective association between protein intake, its interaction with PA, and grip strength (sex- and cohort-specific) was determined using joint models (hierarchical linear mixed effects and a link function for Cox proportional hazards models). Grip strength declined on average by 0·018 SD (95 % CI: –0·026, –0·006) every year. No associations were found between protein intake, measured at baseline, and grip strength, measured prospectively, or rate of decline of grip strength in models adjusted for sociodemographic, anthropometric, lifestyle and health variables (e.g., protein intake ≥ 1·2 v· < 0·8 g/kg aBW/d: β = –0·003, 95 % CI: –0·014, 0·005 SD per year). There also was no evidence of an interaction between protein intake and PA. We failed to find evidence in this study to support the hypothesis that higher protein intake, alone or in combination with higher PA, slowed the rate of grip strength decline in older adults. [ABSTRACT FROM AUTHOR]

Three cycles of NHANES (2013–2018) were used to assess protein intake, protein quality (protein digestibility-corrected amino acid score; PDCAAS), protein cost, and nutrient intakes across quartiles of plant protein intake (Q1: <28.2%; Q2: 28.2% to <32.1%; Q3: 32.1 to <36.2%; Q4: ≥36.2%) over a 24 h period (≥19 years, n = 14 888). Grains represented the primary source of plant protein across quartiles and increased from 5.01% (Q1) to 13.5% (Q4). Across all the USDA's Food and Nutrient Database for Dietary Studies (FNDDS) food groups, grains were the most economical source of protein (24.3–26 g protein/$1). Legumes were the most economical plant-based protein (28–37.7 g protein/$1) within the "Protein Foods" FNDDS group. Absolute protein intake (96.6 g/day) and PDCAAS (0.91) of diets were progressively lower from Q1 to 72.0 g/day and 0.8 in Q4, respectively. Modelling the replacement of 50% of amino acids from grains in the diet with amino acids from legumes increased the PDCAAS by 10% in Q4. Intake levels of fibre, folate, iron, and zinc, were higher in Q2–Q4 compared to Q1 but had lower intakes of vitamins B12 and D. Amino acid complementation is required to effectively integrate higher levels plant protein into US diets. [ABSTRACT FROM AUTHOR]

Ten novel breeding lines of quinoa (Chenopodium quinoa Willd) suitable to be grown in the Pacific Northwest of the United States were developed and utilized for extrusion processing. Understanding how a particular breeding line performs during food processing and which properties determine its performance can promote the use of quinoa as an ingredient in value‐added products, such as extruded snacks. In this study, extrusion characteristics of the whole seed flours of the novel quinoa breeding lines were evaluated using a co‐rotating twin‐screw extruder at two temperatures (110°C and 125°C), three screw speeds (200, 350, and 500 rpm), and a moisture content of 18% (w.b.). The expansion ratio (ER) ranged from 1.15 to 2.33 and was negatively influenced by the fat content in the flours. Breeding line 11WAQ‐104.88 (WAQ10) exhibited the greatest potential for use in direct expanded foods as it had the highest ER for all conditions studied. Strong correlations were found between ER and pasting properties of the flours, proving the usefulness of the pasting test for flour quality evaluation before extrusion processing. The results demonstrate the potential of using quinoa for producing direct expanded food products and highlight the importance of selecting specific breeding lines for desired product characteristics. Practical Application: This study provides the extrusion processing characteristics of 10 new quinoa breeding lines. Based on the information gained, it will be easier for the food industry, including breeders as well as processors, to select the right quinoa variety based on their requirements, and may further help to enhance the use of quinoa. [ABSTRACT FROM AUTHOR]

Increasing market demand for sustainable, environmentally friendly edible film materials has called for the development of new customizable production methods utilizing emerging technologies such as 3D printing. We hereby report a new method to generate functional edible soy protein isolate films prepared from three types of soybeans (AR‐R11‐7999, MO‐S17‐17168, and MO‐S17‐19874R) using an innovative 3D printing technology. The protein contents in AR‐R11‐7999, MO‐S17‐17168, and MO‐S17‐19874R soybean meals and their corresponding protein isolates were 40.0, 39.1, and 39.9; and 84.5, 84.7, and 87.3 % (w/w, dry basis), respectively. Response surface methodology was used to maximize the tensile and puncture strength and minimize the thickness of the 3D‐printed edible films using protein concentration, plasticizer concentration (glycerol), and drying time as the independent variables. The optimized film production conditions were determined as soy protein concentration: 8.91%, plasticizer concentration: 3.00%, and drying time: 3.98 h with a desirability value of 0.7428. The optimized conditions were then successfully verified with the original soybean lot with a nonsignificant difference in physical properties. At the optimized conditions, the 3D‐printed edible films using three soybean lots revealed: 0.108–0.114 mm thickness; 14.79–16.07 MPa tensile strength; 6.97–8.20 N puncture strength; 90.81–91.53, −1.89 to −1.31, and 14.85–17.25 were color parameters L*, a*, and b*, respectively; 1.22–1.36 g/cm3 density; and 104.4–105.7% elongation at break ratio (%). Practical Application: Edible soy protein films produced by an extrusion‐based 3D printing approach are highly customizable and precise, and could be produced at an industrial scale. This newly produced environment‐friendly soy protein‐based edible film can serve as an alternate packaging to synthetic plastics and reduce the environmental landfill problem while adding value to soybean produced in the mid‐south United States. [ABSTRACT FROM AUTHOR]

In the United States, over 70% of milk production is priced under Federal Milk Marketing Orders (FMMOs). A primary purpose of FMMOs is to facilitate orderly allocation of milk as a limited, perishable resource among alternative uses. Fundamental to FMMOs are the regulatory prices applicable to milk used in cheese and whey (Class III), and nonfat dry milk and butter (Class IV). This work examines a novel milk pricing method based on the concept of opportunity cost for milk used in cheese and whey. This novel method may improve the functioning of FMMOs and the U.S. dairy industry. [ABSTRACT FROM AUTHOR]

Beef represents an important source of high-quality dietary protein and several micronutrients including iron, zinc, and B vitamins. Consumption of lean meat including lean beef is recommended by the Dietary Guidelines for Americans 2020-2025 as part of overall healthy diet. Given beef intake has been declining, the objective of this study was to provide updated evaluation of the nutritional contribution of beef types. Twenty-four-hour dietary recall data from adults age 19+ years (n = 19,766) participating in the National Health and Nutrition Examination Survey 2011-2018 was used to assess intakes. On the day of recall, 49.3%, 40.2%, 26.3%, and 15.3% adults consumed total beef, lean fresh beef, ground beef, and processed beef, respectively, with mean intakes of 45.6, 36.6, 21.3, and 6.23 g/day, respectively. Intake of total beef contributed to daily intakes of energy (5.4%), protein (14%), vitamin B 12 (20%), zinc (21%), choline (11%), niacin (9.4%), vitamin B 6 (8.3%), iron (7.6%), phosphorus (6.8%), potassium (5.6%), and magnesium (3%). Lean fresh beef contributed most to the daily intakes of energy and nutrients followed by ground and processed beef. Beef intake also contributed to daily intakes of fat (8.7%), saturated fat (11%), and sodium (2.9%) and lean fresh beef contributed less intakes of fat and saturated fat than ground and processed beef. Beef and particularly lean fresh beef were efficient sources of nutrients and provided more nutrients per 100 kcal than the total diet. In conclusion, based on nutrient contribution, these findings provide evidence to support inclusion of beef (especially lean fresh beef) in dietary recommendations. Contribution of total beef, lean fresh beef, ground beef, and processed beef intake to % daily intake of select nutrients, in adults age 19+ years (n = 19,766): NHANES 2011-2018. [Display omitted] [ABSTRACT FROM AUTHOR]

Purpose: Diet quality is increasingly recognized as important for human reproductive capacity. We studied the association between intake of protein-rich foods and risk of spontaneous abortion (SAB). Methods: During 2013–2020, we recruited pregnancy planners from the United States and Canada (Pregnancy Study Online; PRESTO) and Denmark (SnartForaeldre.dk; SF). Participants completed a baseline questionnaire and a validated cohort-specific food frequency questionnaire. We estimated preconception intake of red meat, poultry, processed meat, seafood, eggs, plant-based proteins, and dairy from individual foods and mixed recipes. We included 4,246 PRESTO and 2,953 SF participants who reported a pregnancy during the study. Data on SAB were derived from questionnaires and population registries. We used Cox proportional hazards regression to estimate hazard ratios (HRs) and 95% confidence intervals (CI), representing the effect of substituting one type of protein-rich food for another. Results: SAB risk was 23% in PRESTO and 16% in SF. In PRESTO, substitution of seafood with other protein-rich foods was associated with higher SAB risk [for example, the HR for replacing 100 g of seafood/week with 100 g of red meat was 1.10 (95% CI 1.00, 1.20)]. In contrast, in SF, substituting seafood with other protein-rich foods was associated with lower SAB risk [HR for replacing 100 g of seafood/week with 100 g of red meat was 0.89 (95% CI 0.82, 0.98)]. Other protein-rich food substitutions were not meaningfully associated with SAB risk. Conclusions: Preconception intake of protein-rich foods was largely unrelated to SAB risk, with the exception of seafood, which was associated with higher risk of SAB in Denmark, but a lower risk in North America. [ABSTRACT FROM AUTHOR]

American football players consume large quantities of animal-sourced protein in adherence with traditional recommendations to maximize muscle development and athletic performance. This contrasts with dietary guidelines, which recommend reducing meat intake and increasing consumption of plant-based foods to promote health and reduce the risk of chronic disease. The capacity of completely plant-based diets to meet the nutritional needs of American football players has not been studied. This modeling study scaled dietary data from a large cohort following completely plant-based diets to meet the energy requirements of professional American football players to determine whether protein, leucine, and micronutrient needs for physical performance and health were met. The Cunningham equation was used to estimate calorie requirements. Nutrient intakes from the Adventist Health Study 2 were then scaled to this calorie level. Protein values ranged from 1.6-2.2 g/kg/day and leucine values ranged from 3.8-4.1 g/meal at each of four daily meals, therefore meeting and exceeding levels theorized to maximize muscle mass, muscle strength, and muscle protein synthesis, respectively. Plant-based diets scaled to meet the energy needs of professional American football players satisfied protein, leucine, and micronutrient requirements for muscle development and athletic performance. These findings suggest that completely plant-based diets could bridge the gap between dietary recommendations for chronic disease prevention and athletic performance in American football players.

Beef is an important source of high-quality protein and several micronutrients, including iron, zinc, and B vitamins. We determined beef intake and its relationship with intakes of nutrients and their adequacy using 24 h dietary recall data from 5868 older adults. Usual intakes from foods were determined using the National Cancer Institute method, and the percent of the population below the estimated average requirement or above adequate intake was estimated. A high percentage of older adults did not meet nutrient recommendations for vitamin D (96%), choline (96%), vitamin E (84%), potassium (70%), calcium (63%), magnesium (60%), vitamin C (46%), vitamin A (39%), zinc (21%), vitamin B 6 (19%), and folate (15%). About 68% of older adults were beef consumers with a mean intake of 56 g/day. Beef consumers had higher ( p < 0.05) intakes of energy, protein, calcium, iron, phosphorus, selenium, sodium, zinc, thiamin, riboflavin, niacin, vitamin B 12 , and choline, and a higher ( p < 0.05) proportion met nutrient recommendations for protein, calcium, copper, zinc, thiamin, folate, and vitamin B 12 than non-consumers. Consumers of fresh, ground, and processed beef also had generally higher intakes and lower inadequacies of many nutrients depending on the beef type. In conclusion, older adults generally had poor nutrient adequacy from their diets, while beef consumers had higher nutrient intakes and adequacy for certain key nutrients, which are inherently generally available from beef or from foods consumed with beef.

Supply chain disruptions, human and animal health concerns, and environmental impacts of livestock production have spurred renewed attention to animal protein consumption in the U.S. Prior research has sought to better understand animal protein consumption by assessing demographic and socioeconomic determinants. However, physical exercise represents a behavioral determinant of consumption that is societally important and, thus far, has not been considered in empirical assessments. Our objective was to quantify the association of exercise with the consumption of total protein, beef, pork, poultry, seafood, eggs, and dairy among U.S. adults. We analyzed 24 h dietary recall and physical activity data from adults in the 2007-2018 National Health and Nutrition Examination Survey (NHANES). The association of exercise with protein consumption (total and disaggregated animal protein) was assessed using ordinary least squares and multivariate Tobit regression. Non-linear associations of exercise with total protein consumption were found, with the magnitude of association highest at 121-180 min per day of exercise. Non-linear associations were also found with animal protein consumption, which differed in sign and magnitude across protein sources. The magnitudes of association, paired with a sizable share of the study sample engaged in exercise, suggest a substantial influence of exercise on protein consumption habits in the U.S.

There is an increasing interest in plant-based diets and higher levels of plant proteins due to rising concerns around health and environmental sustainability issues. We determined the effects of increasing quartiles of plant protein in the diet on nutrient adequacy using a large nationally representative observational dataset. Twenty-four-hour dietary-recall data from NHANES 2013-2018 from 19,493 participants aged 9+ years were used to assess nutrient intakes. Nutritional adequacy was assessed by estimating the percentage of the population with intakes below the EAR or above the AI. A quartile trend was assessed using regression and the significance was set at P quartile trend < 0.05. With increasing quartiles of plant protein, the adequacy decreased for calcium, potassium, and vitamin D and increased for copper and magnesium for adolescents. Among the adults aged 19-50 years, the adequacy decreased for protein, choline, selenium, vitamin B 12 , and zinc and increased for copper, folate, iron, magnesium, thiamin, and vitamin C with increasing quartiles of plant protein. The adequacy for calcium, vitamin A, and zinc decreased and it increased for copper, folate, magnesium, thiamin, and vitamin C with increasing quartiles of plant protein among adults aged 51+ years. The results indicate that diets of mixed protein sources (from both animals and plants) are the most nutritionally adequate.

In wealthy countries, the protein intake of adults is usually considered to be adequate, and considerations of protein quality are often deemed irrelevant. The objective was to examine dietary protein intakes of adults in developed countries in the context of dietary protein quality. An analysis of NHANES population data on actual protein intakes in the United States (a developed country) demonstrated that for a dietary Digestible Indispensable Amino Acid Score (DIAAS) of 100%, 11% of the adult (19-50 y) population had habitual protein intakes below the Estimated Average Requirement (EAR) and 22% below the Recommended Dietary Allowance. The percentage of the population with utilizable protein intakes potentially falling below the EAR increased as the assumed DIAAS declined. Analysis of the NHANES data and several other datasets also indicated that total protein intakes can be limiting or close to limiting for the elderly and some vegetarians and vegans. Here, lower dietary protein quality can potentially lead to inadequate utilizable protein intakes. For many people in specific physiological states (e.g., weight loss, endurance sports, resistance exercise) attempting to meet higher dietary protein targets often with accompanying lowered energy intakes, low dietary protein quality can lead to protein calories expressed as a proportion of total calories, falling outside what may be acceptable limits (maximum of 30% protein calories from total calories). In general, individuals within the adult population may be susceptible to macronutrient imbalance (whenever total protein intakes are high, daily energy intakes low) and for diets with lower protein quality (DIAAS <100%). Our analysis shows that dietary protein quality is relevant in mid- to high-income countries., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Background: Differences in nutrient intakes by urbanization level in the Unites States is not well understood., Objective: Describe, by urbanization level: 1) intake of protein, fiber, percent of energy from added sugars (AS) and saturated fat (SF), calcium, iron, potassium, sodium, and vitamin D; 2) the percent of the population meeting nutrient recommendations., Methods: Twenty-four-hour dietary recalls from 23,107 participants aged 2 y and over from the 2013-2018 National Health and Nutrition Examination Surveys were analyzed. Usual intakes were estimated, and linear regression models adjusted for age, sex, race and Hispanic origin, and whether family income met the 130% threshold examined intake differences by urbanization levels-large urban areas (LUA), small to medium metro areas (SMMA), and rural areas (RA)., Results: A small percentage of the population met the nutrient recommendations, except for protein (92.8%) and iron (70.5%). A higher percentage of the population met recommendations than SMMA and RA for fiber (11.8% compared with 8.1% and 5.3%, P < 0.001), AS (40.2% compared with 33.4% and 31.3%, P < 0.001), SF (26.8% compared with 18.2% and 20.1%, P < 0.001), and potassium (31.5% compared with 25.5% and 22.0%, P < 0.001). Mean protein intake were also higher in LUA than RA (80.0 g compared with 77.7 g, P = 0.003) and fiber intake higher in LUA than SMMA (16.5 g compared with 15.9 g, P = 0.01) and RA (16.5 g compared with 15.2 g, P = 0.001). In addition, contributions to energy intake were lower in LUA than SMMA for AS (11.3% compared with 12.0%, P < 0.001) and SF (11.5% compared with 11.7%, P < 0.001), and for LUA than RA for AS (11.3% compared with 12.9%, P < 0.001) and SF (11.5% compared with 11.8%, P < 0.001)., Conclusions: RA had some markers of poorer diet quality-lower protein and fiber intake and higher AS intake-compared with LUA, and these differences persisted in adjusted regression models. These results may inform public health efforts to address health disparities by urbanization levels in the Unites States., (Published by Elsevier Inc.)

More than 60% of domestic cats in the United States are either overweight or obese (OW). High-protein low-carbohydrate (HPLC) diets have been recommended for weight management for humans and pets. Gut microbes can influence the host's health and metabolism. Less is known about feline gut microbiomes compared to other species. Thirty-nine lean (LN) and OW domestic short-haired cats (median age, 7.2 years) with median body fat of 15.8 and 32.5%, respectively, were enrolled in a two-phase study. All cats were fed the control diet (CON) with 32.4% protein and 32.3% carbohydrate for 8 weeks followed by another 8 weeks of intervention where half of the cats continued the CON diet while the other half were switched to a HPLC diet with 51.4% protein and 11.6% carbohydrate. The goal was to understand how the HPLC diet influenced gut microbiota in obese vs. lean cats. The 16S rRNA gene profiling study revealed a significant impact on gut microbiome by dietary protein and carbohydrate ratio. The effect was more pronounced in OW cats than LN cats. While no microbial taxon was different between groups in LN cats, compositional changes occurred at different taxonomical ranks in OW cats. At the phylum level, Fusobacteria became more abundant in HPLC-fed cats than in CON-fed cats. At the genus level, five short-chain fatty acid (SCFA) producers had altered compositions in response to the diets: Faecalibacterium and Fusobacterium are more abundant in HPLC-fed cats while the abundances of Megasphaera , Bifidobacterium , and Veillonella increased in CON-fed cats. Predicted microbial gene networks showed changes in energy metabolism and one-carbon metabolism pathways. Our study demonstrated differential responses to HPLC diet between obese vs. lean cats and opportunities to explore these SCFA-producers for weight management in cats. [ABSTRACT FROM AUTHOR]

The percentage of broiler complex processing plants slaughtering big birds (e.g., those greater than 3.5 kg) has steadily increased in the United States the past 15 yr. Precision amino acids in dietary formulation is key as the growing cycle for these birds is extended, as well as total feed consumed. Furthermore, broilers reared for big-bird slaughter plants are destined for specialty breast meat yield portioning, making digestible Lys one of the key nutrients in feed formulation. This work assesses dietary Lys levels in graduations across feed phases to 53 D in Cobb MV 3 Cobb 700 female and male broilers. Optimizing digestible Lys in the starter phase (to 14 D) occurred between 1.26 and 1.31%. On average, dietary Lys intake per bird needed to optimize live performance, and white meat yield was higher in male birds (64 g) than in female birds (58 g). Optimization of Lys amino acid density in the Cobb MV 3 Cobb 700 broilers resulted in increased white meat yields in larger broilers, resulting in increased fillet weights per bird with white striping and woody breast being unaffected. [ABSTRACT FROM AUTHOR]

Purpose: This study determined associations between diet quality measured by the Healthy Eating Index (HEI)-2015, physical performance, and successful selection following a U.S. Army Special Forces Assessment and Selection course characterized by arduous cognitive and physical demands. Methods: The HEI-2015 scores were calculated from usual diet assessed with a Block food frequency questionnaire among 782 soldiers attending Special Forces Assessment and Selection. Differences in HEI-2015 scores according to demographics and physical performance were determined with analysis of variance. Differences in likelihood of selection according to HEI-2015 scores were determined with logistic regression. Models were adjusted for potential confounders: age, education, body mass index (BMI), duration and type of resistance training, and smoking. Results: The HEI-2015 total score was higher among older soldiers (≥25 yr), those with more education (≥some college), higher body mass index (≥25), longer duration of resistance training (≥400 min·wk−1), those that reported use of free weights, suspension training, Olympic lifting, and nonsmokers (P < 0.05). The HEI-2015 total score was higher among those with higher Army Physical Fitness Test (APFT) total scores, APFT sit-up score, APFT run score, and faster loaded road march times (P < 0.05). Those with higher HEI-2015 total scores were 75% (quartile 3 vs quartile 1: odds ratio, 1.75; 95% confidence interval, 1.09–2.81) and 65% (quartile 4 vs quartile 1: odds ratio, 1.65, 95% confidence interval, 1.03–2.65) more likely to be selected. Higher scores for total vegetables, greens and beans, seafood and plant protein, and refined grains, but lower sodium scores (indicating more sodium consumed), were associated with better physical performance (P < 0.05). Conclusions: Dietary patterns that conform to federal dietary guidelines (except sodium) are associated with physical performance and Special Forces selection. [ABSTRACT FROM AUTHOR]

High-quality protein feeds are needed in ecological and organic poultry production. Feeds that are rich in methionine (MET) are particularly important because synthetic amino acids are generally prohibited in organic livestock production. Bigheaded carp present an opportunity as feed because they are an invasive fish species that are high in protein and MET. A carp-based meal (CBM) was prepared using innovative dry extrusion, and proximate analysis and nutritional analyses were conducted. The digestible amino acids and true metabolizable energy (TMEn) of the CBM were determined using a colony of cecetomized adult roosters. Starter and grower diets were formulated with CBM and tested in a broiler feeding trial. Diets were high overall in protein, but formulated without synthetic MET. Day-old chicks were randomly assigned to pens of 30 chicks belonging to 1 of 2 treatments (CBM or control containing synthetic MET), with 8 replications. Body weights, feed intake, and feed conversion ratio (FCR) for starter (0–21 d), grower (22–42 d), and overall periods (0–42 d) were analyzed using 1-way ANOVA. The weight gains, feed intake, and FCR did not differ between treatments (P > 0.05) for the overall period (0–42 d). The results indicate that invasive bigheaded carp can be harvested and dry extruded into a novel meal, providing not only a natural and sustainable source of MET, but also an environmental service for damaged aquatic ecosystems in the U.S. [ABSTRACT FROM AUTHOR]

• Near-infrared spectroscopy calibration was developed for mixed seed lots of Camelina sativa. • Morphological differences between biotypes of C. sativa were established. • Morphological changes during plant development have been determined in C. sativa. Camelina [ Camelina sativa (L.) Crantz] has two distinctive biotypes, summer and winter, with winter biotypes requiring a vernalization treatment to enter the reproductive phase. Increased interest in broadening the diversity of winter-hardy cover crops in the northern Great Plains of the U.S. to reduce soil erosion through the winter months has led seed companies to offer winter camelina seed outsourced from other states. Regrettably, in 2017, outsourced camelina seed from other states turned out to be summer biotypes that did not survive the North Dakota winter. The objectives of this study were to determine the morphological characteristics of seed and seedlings from summer- and winter-biotypes. Morphological characteristics of seedlings were determined by growing nineteen summer- and eleven winter-biotypes in an environmental chamber. The experimental design was a randomized complete block design with three replicates. To determine differences in seed wavelength absorbance between winter- and summer-biotypes both visible and near infrared spectra were examined, which encompass 400–2498 nm wavelengths. Mixtures of cultivars Joelle (winter) and one summer type were analyzed using near infrared spectroscopy (NIRS). Seed mixtures were prepared in increments of 5% of 'Joelle'. Mixtures of different lots of Joelle were prepared in increments of 25%. Significant interactions were present and observed for pairs of vegetative leaves, growth stage, height, leaf length, leaf width and the number of lobes. The equation for the ratio of winter seed (r 2 = 0.96) performed very well, being able to distinguish known field grown cultivars used in the equation. Differences in seed and seedling morphological characteristics can be used to differentiate winter- or summer-biotypes. Having a rapid method to determine the percentage of winter camelina in an unknown seed sample will be very favorable for all producers, as well as seed companies, interested in growing winter camelina as either a winter-hardy cover crop or as a winter annual cash crop. [ABSTRACT FROM AUTHOR]

Objective: To examine the relationship between protein intake and the risk of incident premenstrual syndrome (PMS).Design: Nested case-control study. FFQ were completed every 4 years during follow-up. Our main analysis assessed protein intake 2-4 years before PMS diagnosis (for cases) or reference year (for controls). Baseline (1991) protein intake was also assessed.Setting: Nurses' Health Study II (NHS2), a large prospective cohort study of registered female nurses in the USA.ParticipantsParticipants were premenopausal women between the ages of 27 and 44 years (mean: 34 years), without diagnosis of PMS at baseline, without a history of cancer, endometriosis, infertility, irregular menstrual cycles or hysterectomy. Incident cases of PMS (n 1234) were identified by self-reported diagnosis during 14 years of follow-up and validated by questionnaire. Controls (n 2426) were women who did not report a diagnosis of PMS during follow-up and confirmed experiencing minimal premenstrual symptoms.Results: In logistic regression models adjusting for smoking, BMI, B-vitamins and other factors, total protein intake was not associated with PMS development. For example, the OR for women with the highest intake of total protein 2-4 years before their reference year (median: 103·6 g/d) v. those with the lowest (median: 66·6 g/d) was 0·94 (95 % CI 0·70, 1·27). Additionally, intakes of specific protein sources and amino acids were not associated with PMS. Furthermore, results substituting carbohydrates and fats for protein were also null.Conclusions: Overall, protein consumption was not associated with risk of developing PMS. [ABSTRACT FROM AUTHOR]

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This review utilizes current national dietary guidelines and published databases to evaluate the impacts of reasonable shifts in the amount and type of protein intake in the United States on the intersection of human and environmental health. The established scientific basis and recommendations for protein intake as described in the US Dietary Reference Intakes are reviewed. Data on food availability from both the US Department of Agriculture and the Food and Agriculture Organization of the United Nations and data on consumption from the National Health and Nutrition Examination Survey are used to examine estimates of current US protein consumption. Greenhouse gas (carbon dioxide equivalents, CO2eq) and blue and green water impacts of US protein consumption resulting from US agricultural practices were obtained from previously published meta-analyses. A 25% decrease in protein intake paired with a 25% shift from animal food to plant food protein intake—from an 85:15 ratio to a 60:40 ratio—would best align protein intake with national dietary recommendations while simultaneously resulting in 40% fewer CO2eq emissions and 10% less consumptive water use. The modeling of this strategy suggests a savings of 129 billion kilograms of CO2eq and 3.1 trillion gallons of water relative to current consumption. [ABSTRACT FROM AUTHOR]

Traditional foods of indigenous people are a potential untapped source for providing nutritious dietary options in the modern world. The rising popularity of indigenous foods such as amaranth in recent years suggests that these foods can become a popular part of a healthy diet in the United States. Traditional knowledge of indigenous people can form the basis for using wild food resources. This research provides data on 50 plant species common to the central United States that are abundant sources of dietary protein and flber, and used traditionally by Native American tribes. Many species show promise with high amounts of protein and flber being found in lamb's quarters, nettles, grass seeds, and buffalo gourd seeds. These results honor traditional knowledge. In addition, many of these species could be cultivated using techniques similar to those existing for commercial grains and vegetables. Seeds and/or greens of these wild plants could easily be incorporated into the diets of many more people, improving modern diets, and the nutritional quality of food products. [ABSTRACT FROM AUTHOR]

Regulatory frameworks for protein content claims in Canada and the United States are underpinned by the protein efficiency ratio and protein digestibility-corrected amino acid score (PDCAAS), respectively, which are used to assess the protein quality of a given food. The digestible indispensable amino acid score (DIAAS) is a novel approach to measuring the protein quality of foods and is supported by the Food and Agriculture Organization of the United Nations. Methodological concerns about the PDCAAS are addressed by the DIAAS through introduction of the use of ileal amino acid digestibility coefficients and untruncated protein scores. However, before the DIAAS is widely adopted within regulatory frameworks, a comprehensive assessment is required. Accordingly, this review addresses the potential impact of the DIAAS on regulation, communication, and public health, as well as knowledge gaps, analytical challenges, and cost of implementation. A pragmatic approach to addressing protein quality is advocated by suggesting the use of conservative coefficients of digestibility that are derived from in vitro methods. Before adopting the DIAAS as a framework for supporting protein content claims, updated food-related regulations and policies should also be evaluated through a lens that anticipates the impact on consumer-facing nutrition communication, the adoption of dietary patterns that are nutritionally adequate, and a food value chain that fosters a spirit of food and nutritional innovation. [ABSTRACT FROM AUTHOR]

Objectives: To determine the effects of increasing plant-based foods or dairy products on protein intake in older Americans by performing diet modeling. Design: Data from What We Eat in America (WWEIA), the dietary component of the National Health and Nutrition Examination Survey (NHANES), 2007-2010 for Americans aged 51 years and older (n=5,389), divided as 51-70 years (n=3,513) and 71 years and older (n=1,876) were used. Measurements: Usual protein intake was compared among three dietary models that increased intakes by 100%: (1) plant-based foods; (2) higher protein plant-based foods (i.e., legumes, nuts, seeds, soy); and (3) dairy products (milk, cheese, and yogurt). Models (1) and (2) had commensurate reductions in animal-based protein intake. Results: Doubling intake of plant-based foods (as currently consumed) resulted in a drop of protein intake by approximately 22% for males and females aged 51+ years. For older males and females, aged 71+ years, doubling intake of plant-based foods (as currently consumed) resulted in an estimated usual intake of 0.83±0.02 g/kg ideal body weight (iBW))/day and 0.78±0.01 g/kg iBW/day, respectively. In this model, 33% of females aged 71+ years did not meet the estimated average requirement for protein. Doubling dairy product consumption achieved current protein intake recommendations. Conclusion: These data illustrate that increasing plant-based foods and reducing animal-based products could have unintended consequences on protein intake of older Americans. Doubling dairy product intake can help older adults get to an intake level of approximately 1.2 g/kg iBW/day, consistent with the growing consensus that older adults need to consume higher levels of protein for health. [ABSTRACT FROM AUTHOR]

The child welfare court system wrestles consistently with how to measure success. Preventing child deaths, ensuring child safety, enhancing child well-being, and faster permanency are some of the metrics used to assess the effectiveness of our system. This article proposes measuring child welfare courts on their principle-driven expression of values that promote the existence of resilience in families, professionals, and the system itself. It is critical to understand that trauma is not a necessary result of adversity, and the existence of protective factors can make us resilient against the triggering and onset of trauma. Child welfare court systems exist in complexity, and need to be managed for innovation and adaptability. The experience of poverty shared between families and the system is often the curse that weighs against performance, but could be the link that opens expanded capacity. The Court Improvement Training Academy at the University of Washington School of Law provides a framework of values, principles, and examples of work aimed at building resilience-oriented child welfare court systems. [ABSTRACT FROM AUTHOR]

Dynamic crop models that incorporate the effect of environmental variables can potentially explain yield differences associated with location, year, planting date, and cultivars with different growing cycles. Soybean ( Glycine max (L.) Mer.) cultivar coefficients for the DSSAT-CROPGRO model were calibrated from two growing seasons (2012 − 2013) comprising 58 irrigated environments (site × year × planting date combinations) for cultivars within maturity groups (MGs) 3 to 6 using end of season data (yield, seed weight, and seed oil and protein concentration) and previously calibrated phenology coefficients. Model accuracy after calibration of cultivar coefficients by MG (cultivars averaged within a MG) was similar compared to cultivar-specific coefficients. During the subsequent growing season in 2014 (33 environments), the model efficiency (ME) for predicting yield was 0.40, with a root mean square error (RMSE) of 571 kg ha − 1 . The model was less efficient predicting seed number and seed weight (ME = 0.06 and − 0.06, respectively) than yield. The model was able to simulate differences in seed oil concentration across environments and MGs (ME = 0.52), but not protein concentration (ME = − 0.25). The analysis of yield stability had similar slopes for the observed and predicted yield regressions against an observed environmental index (EI) that were only dependent on the MG. Simulated yields were significantly different from the observed when EI > 0, but yield differences in the highest yielding environments were still relatively small (245 to 608 kg ha − 1 ). The results indicate an overall robust model performance in capturing G × E responses with coefficients calibrated by MG. [ABSTRACT FROM AUTHOR]

Meat and poultry are nutrient-dense sources of protein and typically are recommended as part of an overall healthy diet. The objective was to assess the nutritional impact of removing a serving of meat/poultry in Healthy Dietary Patterns (HDPs) using a similar approach to that used by the USDA for Dietary Guidelines for Americans. Composites of minimally processed and further processed meat and poultry were developed and their nutrient profiles were used to accomplish modeling by removing nutrients of each meat and poultry composite from the HDPs. The removal of a 3 oz (85 g) serving of meat or poultry resulted in decreases (10% or more from baseline) in protein and several key micronutrients including iron, phosphorus, potassium, zinc, selenium, thiamine, riboflavin, niacin, vitamin B 6 , vitamin B 12 , and choline as well as cholesterol and sodium in the HDPs, and the decreases were consistent for most nutrients with the removal of either minimally processed (fresh) or further processed meat or poultry and even after adjusting for changes in calories. In conclusion, the results of this dietary modeling study show that the removal of a meat and poultry serving from HDPs resulted in decreases in protein and several key nutrients.

Objective: This study aimed to measure associations between gallbladder disease and protein intake patterns, separated by quantity and type (vegetable vs. animal), among postmenopausal women.Methods: Analyses were based on 130,859 postmenopausal women enrolled from 1993 to 1998 at 40 U.S. clinical centers in the Women's Health Initiative clinical trials and observational study. Women were excluded if they reported a history of gallbladder disease prior to baseline. Cox proportional hazards regression models, adjusted for gallbladder disease risk factors, were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) for associations between energy-adjusted protein intake and gallbladder disease.Results: In this study sample, 8.1% of postmenopausal women self-reported incident gallbladder disease. In multivariate analysis, women in the highest quintile of energy-adjusted vegetable protein intake (>24.0g/d) had a lower risk of gallbladder disease (HR, 0.87; 95% CI, 0.81-0.93) as compared to women in the lowest quintile (<16.3g/d) (Ptrend<0.001). Total protein intake was modestly protective against gallbladder disease (Ptrend<0.021). Animal protein intake was not associated with gallbladder disease risk. The protective effect of vegetable protein held stable only for women without history of diabetes (HR, 0.86; 95% CI, 0.80-0.92) and without recent weight loss (HR, 0.88; 95% CI, 0.80-0.97).Conclusions: Vegetable protein intake is inversely associated with gallbladder disease risk in our sample of postmenopausal women. In addition to weight management, healthcare providers could emphasize vegetable protein as an additional dietary modality to promote lower risk for gallbladder disease. [ABSTRACT FROM AUTHOR]

Objective: To describe the methods, strengths and limitations of available data sources for estimating US meat and protein consumption in order to facilitate accurate interpretations and applications.Design: We examined agricultural supply and dietary intake databases from the US Department of Agriculture (USDA), the US Department of Health and Human Services and the FAO to describe their methodology and to report the most recent estimates for meat and protein consumption.Results: Together, loss-adjusted agricultural supply data and dietary recall data provide the best available estimates of US consumption; the most recent sources indicated that US citizens (ages 2 years and over) consume 4·4-5·9 oz (125·9-166·5 g) of total meat and 6·2-7·4 oz-eq (175·2-209·4 g-eq) from the USDA Protein Foods Group per day. Meat constitutes the majority of intake within the Protein Foods Group, and red meat and processed meat constitute the majority of total meat intake. Nutrient supply data indicate that total meat represents an estimated 43·1 % of the total protein available in the US food supply, but without any loss-adjusted nutrient data, per capita protein intake is best estimated by dietary recall data to be 79·9 g/d.Conclusions: In order to address public health concerns related to excess meat and/or protein consumption, practitioners, educators and researchers must appropriately use available data sources in order to accurately report consumption at the population level. Implications for comparing these estimates with various recommended intakes are discussed. [ABSTRACT FROM AUTHOR]

Background: The impact of protein intake on outcomes in pediatric critical illness is unclear. Objective: We examined the association between protein intake and 60-d mortality in mechanically ventilated children. Design: In a prospective, multicenter, cohort study that included 59 pediatric intensive care units (PICUs) from 15 countries, we enrolled consecutive children (age: 1 mo to 18 y) who were mechanically ventilated for ≥48 h. We recorded the daily and cumulative mean adequacies of energy and protein delivery as a percentage of the prescribed daily goal during the PICU stay ≤10 d. We examined the association of the adequacy of protein delivery with 60-d mortality and determined variables that predicted protein intake adequacy. Results: We enrolled 1245 subjects (44% female) with a median age of 1.7 y (IQR: 0.4, 7.0 y). A total of 985 subjects received enteral nutrition, 354 (36%) of whom received enteral nutrition via the post-pyloric route. Mean ± SD prescribed energy and protein goals were 69 ± 28 kcal/kg per day and 1.9 ± 0.7 g/kg per day, respectively. The mean delivery of enteral energy and protein was 36 ± 35% and 37 ± 38%, respectively, of the prescribed goal. The adequacy of enteral protein intake was significantly associated with 60-d mortality (P < 0.001) after adjustment for disease severity, site, PICU days, and energy intake. In relation to mean enteral protein intake <20%, intake ≥60% of the prescribed goal was associated with an OR of 0.14 (95% CI: 0.04, 0.52; P = 0.003) for 60-d mortality. Early initiation, postpyloric route, shorter interruptions, larger PICU size, and a dedicated dietitian in the PICU were associated with higher enteral protein delivery. Conclusions: Delivery of >60% of the prescribed protein intake is associated with lower odds of mortality in mechanically ventilated children. Optimal prescription and modifiable practices at the bedside might enhance enteral protein delivery in the PICU with a potential for improved outcomes. This trial was registered at clinicaltrials.gov as NCT02354521. [ABSTRACT FROM AUTHOR]

ObjectiveTo assess the change in daily energy intake associated with pairwise compositional change in carbohydrate, fat and protein intake among US adults stratified by sex, race/ethnicity and weight status.DesignLinear mixture model was performed to estimate the relationship between daily energy intake and macronutrient composition, adjusted for age and alcohol consumption, and accounting for survey design.SettingStudy sample from the National Health and Nutrition Examination Survey, 1999–2010 waves.SubjectsA total of 27 589 US adults aged 20 years and older were included in the study. Dietary macronutrient intake was calculated from 24 h dietary recall and BMI from objectively measured weight/height.ResultsAcross all population subgroups, substituting protein or carbohydrate for fat and substituting protein for carbohydrate were associated with decreased daily energy intake, with the largest effect resulting from substituting protein for fat. A 1 % increase in the percentage of energy from protein substituted for a 1 % decrease in the percentage of energy from fat was associated with a decrease in daily energy intake of 268·2 (95 % CI 169·0, 367·4) kJ, 289·5 (95 % CI 215·9, 363·2) kJ and 293·7 (95 % CI 210·0, 377·4) kJ among normal-weight (18·5≤BMI, kg/m2<25·0), overweight (25·0≤BMI, kg/m2<30·0) and obese (BMI≥30·0 kg/m2) men, and 177·4 (95 % CI 130·5, 224·3) kJ, 188·7 (95 % CI 139·3, 238·1) kJ and 204·2 (95 % CI 158·2, 250·2) kJ among normal-weight, overweight and obese women, respectively. The relationship between macronutrient composition and daily energy intake varied substantially across sex, race/ethnicity and weight status.ConclusionsPolicies promoting higher daily protein intake at the expense of lower fat intake could be effective in reducing total energy intake among US adults. [ABSTRACT FROM AUTHOR]

Objective: Evaluate reported use of the practice of "eating more protein" to prevent weight gain among midlife women. Design: Cross-sectional national survey. Participants: One thousand eight hundred twenty-four midlife women (40-60 y) from the 9 United States geographic regions, primarily married (71 %), white (76%), and well educated; half were premenopausal (49%). Outcomes: Frequency of dietary practices to prevent weight gain, Weight Efficacy Lifestyle score, self-reported weight change and body mass index over the past 2 years, and current protein intake. Analysis: Linear regression models determined associations between weight change, protein intake, and reported use of the practice of "eating more protein" to prevent weight gain. Results: Most women correctly identified good protein sources, and the majority could indicate the daily percent dietary energy recommended from protein. "Eating more protein" to prevent weight gain was reported by 43% of women as a practice to prevent weight gain and was associated with weight loss over a 2-year period and with increased percent energy from protein. Conclusions and Implications: Reported use of the practice of "eating more protein" was associated with weight loss over 2 years. Education regarding dietary protein requirements may enhance the use of this practice. [ABSTRACT FROM AUTHOR]

Abstract: The crayfish industry in Louisiana is the largest in the United States, with crayfish frequently harvested from waters that experience episodic or chronic hypoxia (dissolved oxygen [DO]≤2mg/l). We examined physiological biomarkers (hemolymph lactate, glucose, and protein concentrations) of hypoxic stress in the red swamp crayfish Procambarus clarkii from chronically hypoxic natural habitats and laboratory hypoxia experiments. P. clarkii from normoxic and hypoxic areas in the Atchafalaya River Basin were sampled monthly from April to July 2010. Laboratory experiments subjected P. clarkii to severe hypoxia (1mg/l DO), moderate hypoxia (2mg/l DO), or normoxic conditions (control: DO>7.5mg/l) for 12, 24, and 48h. P. clarkii from normoxic and hypoxic natural habitats did not display significantly different hemolymph lactate or glucose concentrations; however, mean hemolymph protein concentration was significantly lower in crayfish from hypoxic areas. P. clarkii exposed to severe hypoxia in laboratory experiments had significantly higher hemolymph lactate and glucose concentrations for all three exposure times, whereas large differences in protein concentrations were not observed. These results suggest that elevated hemolymph lactate and glucose concentrations are responses to acute hypoxia in P. clarkii, while differences in protein concentrations are the result of chronic hypoxic exposure. [Copyright &y& Elsevier]

The NUTRIMENTHE project is in receipt of €5.9 million from the European Commission's Seventh Framework Programme to study the effect of diet on the mental performance of children. The project is especially interested in the effect of B-vitamins, protein (in breast and formula milk), minerals and long-chain polyunsaturated fatty acids on the six main domains of mental performance. NUTRIMENTHE is also researching consumer perceptions of how diet affects mental performance and the economic consequence of improving mental performance. This article provides an insight into the results and progress made to date by the project. [ABSTRACT FROM AUTHOR]

Owing to the complex interactions among climate, plants, cattle grazing, and land management practices, the impacts of climate change on cattle have been hard to predict. Predicting future grassland ecosystem functioning relies on understanding how changes in climate alter the quantity of forage produced, but also forage quality. Plant protein, which is a function of plant nitrogen concentrations, and digestible energy limit the performance of herbivores when in short supply; moreover, deficiencies can be expensive to mitigate. To better understand how changes in temperature and precipitation would affect forage protein and energy availability, we analyzed over 21 000 measurements of cattle fecal chemistry acquired over 14 years in the continental US. Our analysis of patterns in forage quality among ecologically defined regions revealed that increasing temperature and declining precipitation decreased dietary crude protein and digestible organic matter for regions with continental climates. Within regions, quality also declined with increased temperature; however, the effects of precipitation were mixed. Any future increases in precipitation would be unlikely to compensate for the declines in forage quality that accompany projected temperature increases. As a result, cattle are likely to experience greater nutritional stress in the future. If these geographic patterns hold as a proxy for future climates, agriculture will require increased supplemental feeds or the consequence will be a decrease in livestock growth. [ABSTRACT FROM AUTHOR]

There is still much uncertainty as to how wildfire affects the accumulation of burn residues (such as black carbon (BC)) in the soil, and the corresponding changes in soil organic carbon (SOC) composition in boreal forests. We investigated SOC and BC composition in black spruce forests on different landscape positions in Alaska, USA. Mean BC stocks in surface mineral soils (0.34 ± 0.09 kg C m) were higher than in organic soils (0.17 ± 0.07 kg C m), as determined at four sites by three different C Nuclear Magnetic Resonance Spectroscopy-based techniques. Aromatic carbon, protein, BC, and the alkyl:O-alkyl carbon ratio were higher in mineral soil than in organic soil horizons. There was no trend between mineral soil BC stocks and fire frequencies estimated from lake sediment records at four sites, and soil BC was relatively modern (<54–400 years, based on mean ΔC ranging from 95.1 to −54.7‰). A more extensive analysis (90 soil profiles) of mineral soil BC revealed that interactions among landscape position, organic layer depth, and bulk density explained most of the variance in soil BC across sites, with less soil BC occurring in relatively cold forests with deeper organic layers. We suggest that shallower organic layer depths and higher bulk densities found in warmer boreal forests are more favorable for BC production in wildfire, and more BC is integrated with mineral soil than organic horizons. Soil BC content likely reflected more recent burning conditions influenced by topography, and implications of this for SOC composition (e.g., aromaticity and protein content) are discussed. [ABSTRACT FROM AUTHOR]

There has been considerable interest in recent years in the development of miniaturized and parallelized array technology for protein–protein interaction analysis and protein profiling, namely ‘protein-detecting microarrays’. Protein-detecting microarrays utilize a wide variety of capture agents (antibodies, fusion proteins, DNA/RNA aptamers, synthetic peptides, carbohydrates, and small molecules) immobilized at high spatial density on a solid surface. Each capture agent binds selectively to its target protein in a complex mixture, such as serum or cell lysate samples. Captured proteins are subsequently detected and quantified in a high-throughput fashion, with minimal sample consumption. Protein-detecting microarrays were first described by MacBeath and Schreiber in 2000, and the number of publications involving this technology is rapidly increasing. Furthermore, the first multiplex immunoassay systems have been cleared by the US Food and Drug Administration, signaling recognition of the usefulness of miniaturized and parallelized array technology for protein detection in predictive/early diagnosis. Although genetic tests still predominate, with further development protein-based diagnosis will become common in clinical use within a few years. [ABSTRACT FROM AUTHOR]

Protein and oil are the most important components of soybean [Glycine max (L.) Merr.], and they have been shown to have an inverse relationship. As there is a growing demand for soybean oil-based diesel as an alternative fuel source, a better understanding of the protein- oil inverse relationship in conjunction with seed yield could be useful in determining the potential of soybean as a source of biodiesel. We retrospectively examined advanced soybean breeding lines from multiple breeding programs across the southern USA to determine associations between protein and oil and seed yield. The data analyzed were a subset of the Uniform Soybean Tests and consisted of 31 different soybean breeding lines and four checks grown at 10 locations in 2000, six locations in 2001, and eight locations in 2002. A progressive multiple regression analysis was used to develop a single equation describing seed yield over all entries, locations, and years as a function of five traits: oil content, protein content, maturity, lodging, and plant height. From this equation, the optimum plant type for maximum seed yield in the southern USA had either high oil (225 g kg-1) and low protein (385 g kg-1), or high protein (437 g kg-1) and low oil (190 g kg-1). Seed yield continues to be the dominant trait of selection in soybean breeding programs. This study demonstrates that high yield can be achieved with either high protein or high oil. Hence, there is potential for maintaining yield while improving soybean cultivars for oil production. Given the energy balance between fossil-derived and soy-derived diesel, if soy-based biodiesel is to be a significant option, an increased focus on developing high-oil/high-yield soybean needs to occur. [ABSTRACT FROM AUTHOR]

The effects of forage source, concentration of metabolizable protein (MP), type of carbohydrate, and their interactions on nutrient digestibility and production were evaluated using a central composite treatment design. All diets (dry basis) contained 50% forage that ranged from 25:75 to 75:25 alfalfa silage:corn silage. Rumen-degradable protein comprised 10.7% of the dry matter (DM) in all diets, but undegradable protein ranged from 4.1 to 7.1%, resulting in dietary MP concentrations of 8.8 to 12.0% of the DM. Dietary starch ranged from 22 to 30% of the DM with a concomitant decrease in neutral detergent fiber concentrations. A total of 15 diets were fed to 36 Holstein cows grouped in 6 blocks. Each block consisted of three 21-d periods, and each cow was assigned a unique sequence of 3 diets, resulting in 108 observations. Milk production and composition, feed intake, and digestibility of major nutrients (via total collection of feces and urine) were measured. Few significant interactions between main effects were observed. Starch concentration had only minor effects on digestibility and production. Replacing corn silage with alfalfa decreased digestibility of N but increased digestibility of neutral detergent fiber. Increasing the concentration of MP increased N digestibility. The concentration (Mcal/kg) of dietary digestible energy (DE) increased linearly as starch concentration increased (very small effect) and was affected by a forage by MP interaction. At low MP, high alfalfa reduced DE concentration, but at high MP, increasing alfalfa increased DE concentration. Increasing alfalfa increased DM and DE intakes, which increased yields of energy-corrected milk, protein, and fat. Increasing MP increased yields of energy-corrected milk and protein. The response in milk protein to changes in MP was much less than predicted using the National Research Council (2001) model. [ABSTRACT FROM AUTHOR]

Abstract: Objective: We examined the associations of total calcium intake (TCI) and dietary protein intake (DPI) with risk of fracture. Methods: A total of 2006 postmenopausal women ≥50 y of age who were measured in the 1999–2002 National Health and Nutrition Examination Survey were included in the study. Weighted mean TCI and DPI and percentage of distributions of selected characteristics were estimated by TCI category and fracture status. Multivariate logistic regression models were used to assess the effect of TCI and DPI on risk of fracture. Results: Thirteen percent of participants reported a fracture history, of whom 17.8% consumed a total of ≥1200 mg of calcium per day and 23.8% consumed <400 mg/d. TCI was not associated with fracture risk when controlling for all selected covariates. In women who consumed <46 g/d of dietary protein, those with a TCI ≥1200 mg/d had a significantly higher risk of fracture than those with the lowest TCI (adjusted odds ratio 5.98, 95% confidence interval 1.15–31.13), whereas in women who consumed >70 g/d of dietary protein, those with a TCI ≥1200 mg/d had an insignificant lower risk of fracture (adjusted odds ratio 0.69, 95% confidence interval 0.20–2.39). Conclusion: TCI is not associated with risk of fracture among postmenopausal women. Adequate TCI in the presence of inadequate DPI may not be protective against fractures. Optimal proportion of TCI and DPI warrants further investigation among older women. [Copyright &y& Elsevier]

Quality of soybeans and soybean meals (SBM) from non-US and US origins were compared. The US SBM was more consistent with higher digestibility (by KOH solubility), lower fiber and better quality of protein (by essential amino acid levels) than SBM of other major export origins (Argentina, Brazil and India). Protein quality carried through from whole soybeans to SBM, for a given origin. While the protein content was higher for the SBM from Brazil, the percentage of total digestible amino acids was highest for the SBM from the US and China. The US SBM had the highest content (total) of five essential amino acids for both poultry and swine feed uses, which when coupled with higher digestibility, would give US meal an advantage in rations balanced on amino acids. The mean particle size for all SBM from all origins was within the desirable range specified by nutritionists. The US soybeans were lower in protein than Brazilian soybeans, but higher than Argentine soybeans. The crude protein disadvantage of US soybeans was offset by higher concentrations of the essential amino acids in the lower protein soybeans. The US soybeans were lower in oil content than soybeans from either of the South American origins. Average protein and oil contents of US soybeans were consistent with the 2003 and 2004 annual soybean quality surveys. The US SBM held an advantage in digestibility and concentration of key amino acids in all three studies. [ABSTRACT FROM AUTHOR]

The relationships among BW, backfat depth, and body physical and chemical composition were evaluated in response to dietary protein and DE balance in breeding gilts from 30 kg of BW to weaning of the first litter. Large White (sire) x Landrace (dam) F1 hybrid (White; n = 75) and Landrace (sire) x (Meishan x Large White; dam) (Meishan; n = 19) hybrid gilts were received at 30 kg of BW. Five gilts were taken as the initial slaughter group at 30 kg of BW, and the remaining gilts were fed diets differing in total lysine to DE ratio, high (H) vs. low (L), from 30 kg of BW to mating (rearing), and during gestation and lactation, allowing factorial investigation of dietary treatment effects and interactions during rearing, gestation, and lactation. Gilts were slaughtered at approximately 50 and 90 kg of BW, and at mating, farrowing, and weaning. Gilts fed L diets during rearing were lighter at mating (117.9 vs. 133.6 kg of BW, P = 0.035) due to a reduction in gain (592 vs. 720 g/d, P = 0.002) and a restriction in protein accretion (83 vs. 117 g/d, P = 0.001). During rearing, lipid accretion did not differ between L- and H-fed gilts (208 vs. 198 g/d, P = 0.60), but the ratio of lipid to protein accretion was about 1.5-fold greater in L-fed gilts, where lipid mass expressed as a percentage of BW was increased at mating (26.0 vs. 21.9%, P = 0.005). Effects of L diets on lipid accretion during rearing were transient; no residual effects on body lipid mass (P > 0.17) were found at farrowing or weaning. Overall, Meishan hybrids carried greater lipid mass (P < 0.001) than White hybrid gilts. Whereas the rate of body lipid and protein accretion and body lipid and protein mass can be nutritionally influenced and can vary according to growth stage, reproductive status, and genotype, this study established that body protein mass expressed as a proportion of the lipid free empty BW remains inflexible. A value for this measure of 0.188 ± 0.0052 was found in White and Meishan hybrid gilts ranging from 28 to 203 kg of BW and 3 to 36 mm backfat depth, covering growth, pregnancy, and lactation, and offered diets differing in protein and energy balance. Body protein mass can be predicted as approximately 0.2 of the lipid free empty BW once body lipid mass is estimated accurately from physical measurements, such as backfat depth (P2, mm) and BW (kg), by regression using lipid (kg) = -8.14 (SE, 1.302) + 0.167 (SE, 0.010) BW + 0.883 (SE, 0.065) P2 (residual SD = 3.51; R² = 0.912). [ABSTRACT FROM AUTHOR]

The objective of this study was to determine if a reduction in dietary CP, with partial replacement of the intact protein with crystalline AA (CAA), would alter growth, morphology, and free or peptide-bound AA concentrations of intestinal mucosa in growing pigs. Twenty-four barrows (37.0 ± 1.5 kg of BW) were fed 1 of 4 diets for 24 d: 16.1% CP with no CAA, or 12.8, 10.1, or 7.8% CP (analyzed values, as-fed) containing CAA. As CP decreased, CAA were gradually increased to meet requirements on a true ileal digestible basis. Pigs were euthanized 2 h postmeal on d 24, and mucosal samples from duodenum, jejunum, and ileum were collected. Reducing dietary CP decreased ADG, G:F, and final weight (linear, P < 0.05). With reduced dietary CP, mucosal protein concentration decreased in the jejunum (quadratic, P < 0.05) and tended to decrease in the ileum (linear, P = 0.062). Reduction of the dietary CP concentration from 16.1 to 7.8% tended to decrease the crypt depth (linear, P < 0.10) and decreased villus width (linear, P < 0.05) in duodenum and jejunum mucosa but did not reduce villus height or villus surface area in any regions of the small intestine. In the duodenum, a reduction in dietary CP increased free Lys, Met, and Thr (linear, P < 0.05) and peptide-bound Lys and Thr (quadratic, P < 0.10). In the jejunum, reducing CP decreased free Cys (linear P < 0.05) and tended to decrease free Asn and His (linear, P < 0.10) and peptide-bound His (quadratic, P = 0.061) and Ile, Leu, and Val (linear, P < 0.10). In the ileum, reducing CP decreased free Asn, Ser, Tyr, Arg, His, Phe (linear, P < 0.05), and Leu (linear, P = 0.054) and peptide-bound Gly and Ser (linear, P < 0.05) and tended to decrease peptide-bound Ile, Leu, Phe, Val (linear, P < 0.10), and Lys (linear P < 0.05). In conclusion, reduced-CP diets supplemented with CAA lead to a reduction in growth performance, associated with biochemical and morphological modifications of the intestinal mucosa. [ABSTRACT FROM AUTHOR]

Summary • The likely consequences of future high levels of atmospheric CO2 concentration on wheat (Triticum aestivum L.) grain nutritional and baking quality were determined. • Two free-air CO2 enrichment (FACE; 550 mmol mol-1 ) experiments were conducted at ample (Wet) and limiting (Dry) levels of irrigation, and a further two experiments at ample (High-N) and limiting (Low-N) nitrogen concentrations. Harvested grain samples were subjected to a battery of nutritional and bread-making quality tests. • The Dry treatment improved grain quality slightly (protein +2%; bread loaf volume +3%). By contrast, Low-N decreased quality drastically (protein -36%; loaf volume -26%). At ample water and N, FACE decreased quality slightly (protein -5%; loaf volume -2%) in the irrigation experiments and there was no change in the nitrogen experiments. At Low-N, FACE tended to make the deleterious effects of Low-N worse (protein -33% and -39%, at ambient CO2 and FACE, respectively; loaf volume -22% and -29% at ambient CO2 and FACE, respectively). • The data suggest that future elevated CO2 concentrations will exacerbate the deleterious effects of low soil nitrogen on grain quality, but with ample nitrogen fertilizer, the effects will be minor. [ABSTRACT FROM AUTHOR]

With the growing interest in non-dairy products, there has been a surge of interest in consumers seeking plant-based cheese alternatives spurred by a desire to improve individual health and achieve a more sustainable food supply. The aim of this study was to conduct a cross-sectional survey of non-dairy cheese alternatives available in the United States and to evaluate their nutritional content. A total of 245 non-dairy plant-based cheese alternatives were analyzed using their nutritional facts labels. The various cheese alternatives were based upon coconut oil ( n = 106), cashews and coconut ( n = 61), cashews ( n = 35), oats ( n = 16), almonds ( n = 7), soy ( n = 6), palm fruit oil ( n = 5), and other blends ( n = 9). Only 3% of these cheese alternatives had 5 g or more of protein, while 19%, 14%, and 1% were fortified with calcium, vitamin B12, and vitamin D, respectively. Almost 60% had high levels of saturated fat, while 15% had low sodium levels. The products based on cashews alone more commonly had the highest protein levels and the lowest sodium and saturated fat levels. Those containing coconut oil more commonly had higher saturated fat and sodium levels and were most frequently fortified with vitamin B12. Few of these products could be considered good dietary sources of either protein or calcium.

Dietary supplements (DSs) are used by 50% of Americans and 70% of United States military service members (SMs); some have adverse effects (AEs). This cross-sectional investigation examined AEs associated with specific DSs. A stratified random sample of SMs from the Air Force, Army, Marine Corps, and Navy was obtained. Volunteers completed a questionnaire reporting AEs for 96 generic and 62 specific DSs. The highest prevalence (≥1 AE) in specific DS categories was 35% prohormones, 33% weight loss supplements, 26% pre/post workout supplements, 14% herbal products, 12% multivitamin/multiminerals, 11% protein/amino acids, 9% muscle building supplements, 7% other DSs, 6% joint health products, and 5% individual vitamins/minerals. Specific DSs of concern (with proportion reporting AEs) included: Libido Max® (35%), Hydroxycut Hardcore® (33%), OxyElite® (33%), Roxylean® (31%), Growth Factor 9® (30%), Super HD® (29%), Hydroxycut Advanced® (29%), Lipo 6® (28%), The Ripper® (27%), Test Booster® (27%), Xenadrine Xtreme Thermogenic® (27%), C4 Extreme® (26%), and C4 Original® (25%). Products marketed for weight loss, use before/after workout, and prohormones had the highest AE prevalence. DSs can contain substances with independent/additive AEs and/or interact with other ingredients or prescribed medications. Methods described here could provide a continuous surveillance system detecting dangerous DSs entering the market., (Published by Elsevier Ltd.)

Food insecurity affects an estimated 12% of households in the United States. Adults and children who experience food insecurity are increased risk for development of metabolic diseases such as type 2 diabetes, obesity, and cardiovascular disease. The negative health outcomes associated with food insecurity are multifactorial; however, many of them may be caused by limited nutritional intake and poor diet quality. Dietary intake of eggs may be an applicable solution for food-insecure families who are challenged by limited nutritional intake. Eggs contain a variety of nutrients that support metabolic health. For instance, eggs are a complete source of high-quality protein and contain 16 vitamins and minerals. Furthermore, eggs are cost efficient. When comparing the relationship between foods on the basis of calories and unit cost, the energy cost of eggs is significantly less when compared with that of other animal-protein foods such as meat, poultry, and fish. However, dietary intake of eggs is controversial in regard to cardiovascular health. Thus, the aim of this review is to summarize the role of eggs in the diet and the impact eggs have on health for adults and children living in a food-insecure environment., (© The Author(s) 2021. Published by Oxford University Press on behalf of the International Life Sciences Institute. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Yogurt is considered a healthy, nutritious food in many cultures. With a significant number of people experiencing dairy intolerance, and support for a more sustainable diet, consumer demand for dairy alternatives has surged. The aim of this study was to conduct a cross-sectional survey of plant-based yogurt alternatives to assess their nutritional content and health profile. A total of 249 non-dairy yogurt alternatives were analyzed from the nutrition label listed on the commercial package. The various yogurt alternatives contained extracts of coconut ( n = 79), almonds ( n = 62), other nuts or seeds ( n = 20), oats ( n = 20), legumes ( n = 16), and mixed blends ( n = 52). At least one-third of the yogurt alternatives had 5 g or more of protein/serving. Only 45% of the yogurt alternatives had calcium levels fortified to at least 10% of daily value (DV), while only about one in five had adequate vitamin D and B12 fortification at the 10% DV level. One-half of the yogurt alternatives had high sugar levels, while 93% were low in sodium. Except for the coconut-based products, the yogurts were not high in fat or saturated fat. The yogurt alternatives were not fortified as frequently or to the same levels as the corresponding non-dairy, plant-based beverages.