1. Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis).
- Author
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Raulf-Heimsoth M, Rihs HP, Rozynek P, Cremer R, Gaspar A, Pires G, Yeang HY, Arif SA, Hamilton RG, Sander I, Lundberg M, and Brüning T
- Subjects
- Adolescent, Adult, Antigens, Plant biosynthesis, Antigens, Plant genetics, Antigens, Plant immunology, Carbohydrates immunology, Child, Child, Preschool, Cross Reactions immunology, Epitopes immunology, Female, Germany, Health Personnel, Hevea chemistry, Hevea genetics, Humans, Immunoglobulin E blood, Latex Hypersensitivity blood, Latex Hypersensitivity immunology, Male, Middle Aged, Portugal, Recombinant Proteins biosynthesis, Recombinant Proteins immunology, Spinal Dysraphism complications, United States, Antigen-Antibody Reactions immunology, Immunoglobulin E immunology, Latex Hypersensitivity diagnosis, Rubber
- Abstract
Background: Characterized native and recombinant Hevea brasiliensis (rHev b) natural rubber latex (NRL) allergens are available to assess patient allergen sensitization profiles., Objective: Quantification of individual IgE responses to the spectrum of documented NRL allergens and evaluation of cross-reactive carbohydrate determinants (CCDs) for more definitive diagnosis., Methods: Sera of 104 healthcare workers (HCW; 51 German, 21 Portuguese, 32 American), 31 spina bifida patients (SB; 11 German, 20 Portuguese) and 10 Portuguese with multiple surgeries (MS) were analysed for allergen-specific IgE antibody (sIgE) to NRL, single Hev b allergens and CCDs with ImmunoCAP technology., Results: In all patient groups rHev b 5-sIgE concentrations were the most pronounced. Hev b 2, 5, 6.01 and 13 were identified as the major allergens in HCW and combined with Hev b 1 and Hev b 3 in SB. In MS Hev b 1 displayed an intermediate relevance. Different sIgE antibody levels to native Hevea brasiliensis (nHev b) 2 and rHev b 6.01 allowed discrimination of SB with clinical relevant latex allergy vs. those with latex sensitization. Sensitization profiles of German, Portuguese and American patients were equivalent. rHev b 5, 6.01 and nHev b 13 combined detected 100% of the latex-allergic HCW and 80.1% of the SB. Only 8.3% of the sera showed sIgE response to CCDs., Conclusions: Hev b 1, 2, 5, 6.01 and 13 were identified as the major Hev b allergens and they should be present in standardized latex extracts and in vitro allergosorbents. CCDs are only of minor relevance in patients with clinical relevant latex allergy. Component-resolved diagnostic analyses for latex allergy set the stage for an allergen-directed immunotherapy strategy.
- Published
- 2007
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