1. Human papillomavirus is not associated with colorectal cancer in a large international study.
- Author
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Gornick, Michele, Castellsague, Xavier, Sanchez, Gloria, Giordano, Thomas, Vinco, Michelle, Greenson, Joel, Capella, Gabriel, Raskin, Leon, Rennert, Gad, Gruber, Stephen, Moreno, Victor, Gornick, Michele C, Giordano, Thomas J, Greenson, Joel K, and Gruber, Stephen B
- Subjects
ADENOCARCINOMA ,COLON tumors ,DNA ,PAPILLOMAVIRUS diseases ,POLYMERASE chain reaction ,RECTUM tumors ,RESEARCH funding ,VERTEBRATES ,VIRUS diseases ,CASE-control method ,NEOPLASTIC cell transformation ,DISEASE complications - Abstract
Objective Of the Study: Recent publications have reported an association between colon cancer and human papillomaviruses (HPV), suggesting that HPV infection of the colonic mucosa may contribute to the development of colorectal cancer.Methods: The GP5+/GP6+ PCR reverse line blot method was used for detection of 37 types of human papillomavirus (HPV) in DNA from paraffin-embedded or frozen tissues from patients with colorectal cancer (n = 279) and normal adjacent tissue (n = 30) in three different study populations, including samples from the United States (n = 73), Israel (n = 106) and Spain (n = 100). Additionally, SPF10 PCR was run on all samples (n = 279) and the Innogenetics INNO-LiPA assay was performed on a subset of samples (n = 15).Results: All samples were negative for all types of HPV using both the GP5+/GP6+ PCR reverse line blot method and the SPF10 INNO-LiPA method.Conclusions: We conclude that HPV types associated with malignant transformation do not meaningfully contribute to adenocarcinoma of the colon. [ABSTRACT FROM AUTHOR]- Published
- 2010
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