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Abstract: The chemical constituents from Aconitum richardsonianum var. pseudosessiliflorum were investigated. The roots of this plant were extracted three times with 90% EtOH at the room temperature. The ethanol extracts were combined and concentrated under reduced pressure to yield residue, which was suspended in water and successively partitioned with chloroform. The chloroform extraction was isolated and purified by silica gel and Sephadex LH-20 column chromatography. Six compounds were isolated and elucidated as delelatine (1), isodelpheline (2), 3-acetylaconitine (3), isoatisine (4), nordhagenine A (5) and yunaconitine (6). Compounds 1–5 were obtained from Aconitum Brunneum for the first time. Compound (1) showed significant cytotoxic activities (IC50=4.36μM) against the human tumor cell line P388. [Copyright &y& Elsevier]