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A novel Gram-stain-negative, aerobic, rod-shaped bacterium named T808 T was isolated from an alpine soil in Qamdo, Tibet, PR China. Strain T808 T grew at 5-30℃, pH 5.0-9.0 (optimum, 25℃ and pH 7.0-8.0) with 0-2% (w/v) NaCl (optimum, 0%). The 16S rRNA gene sequences of strain T808 T showed the highest similarity with Pararhizobium herbae CCBAU83011 T (98.8%), followed by Pararhizobium polonicum F5.1 T (98.7%), Pararhizobium giardinii H152 T (98.5%), Rhizobium gei ZFJT-2  T (98.4%), and Pararhizobium antarcticum NAQVI59 T (97.5%). The highest digital DNA-DNA hybridization (dDDH), core-proteome average amino acid identity (cpAAI) and average nucleotide identity (ANI) values between strain T808 T and related strains were estimated as 28.0%, 92.1% and 84.4%, respectively. Phylogenetic analysis based on 16S rRNA, core-proteome and whole-genome indicated that strain T808 T belonged to the genus Pararhizobium. The genome size was 6.24 Mbp with genomic DNA G + C content of 60.1%. The major cellular fatty acids were Summed feature 8 (C 18:1 ω7c or C 18:1 ω6c), C 16:0 and C 19:0 cyclo ω8c. The polar lipids were diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline and unidentified aminophospholipid. The isoprenoid quinone were ubiquinone-10 and ubiquinone-9. Based on phenotypic, phylogenetic, and genotypic data, strain T808 T is considered to represent a novel species of the genus Pararhizobium, for which the name Pararhizobium qamdonense sp. nov. is proposed. The type strain is T808 T (= JCM 36247  T  = CICC 25216  T ). According to phylogenetic coherence based on 16S rRNA, core-proteome and whole-genome, it is also proposed that the type strain Rhizobium gei Shi et al. 2016 should be reclassified as Pararhizobium gei comb. nov., the type strain is ZFJT-2  T (= CCTCC AB 2013015  T  = KCTC 32301  T  = LMG 27603  T )., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

A Gram-stain-negative, light yellow, aerobic, non-motile, short rod-shaped bacterium named strain Y-23 T with iprodione-degrading capability was isolated from a soil under a greenhouse in Tibet, PR China. Strain Y-23 T grew at 4-37 ℃ and pH 5.0-9.0 (optimum, 25 ℃ and pH 7.0) with 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and chromosome genome indicated that strain Y-23 T formed a stable evolutionary branch with Acinetobacter tandoii DSM 14970 T . The 16S rRNA gene similarity, digital DNA-DNA hybridization and average nucleotide identity values between strain Y-23 T and Acinetobacter tandoii DSM 14970 T were 98.31%, 43.2% and 91.2%, respectively. The genome size was 3.39 Mbp with a genomic DNA G+C content of 40.59 mol%. The predominant fatty acids were C 18:1 ω9c, Summed feature 3 (C 16:1 ω7c/C 16:1 ω6c), C 12:0 , C 12:0 3-OH and C 16:0 . The polar lipids were diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidyl choline, unidentified phospholipid, four unidentified aminophospholipids and two unidentified lipids. The isoprenoid quinone was Q-8 (19.43%) and Q-9 (80.57%). Based on phenotypic, phylogenetic, and genotypic data, strain Y-23 T is considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter tibetensis sp. nov. is proposed. The type strain is Y-23 T (= CICC 25150 T  = JCM 35630 T )., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)