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Plateau lakes characterized by salinization and eutrophication are essential aquatic ecosystems. A myriad of microorganisms serve as crucial biological resources in plateau lakes and drive the elemental cycles of these ecosystems. Currently, there is a paucity of knowledge regarding the impacts of salinization and eutrophication dynamics on the microbiota in plateau lakes. Here, high-throughput sequencing of the 16S ribosomal RNA genes (V4 region) was used to characterize microbial community structure and assembly in plateau lakes with different salinities and trophic levels. Water samples were collected at 191 sites across 24 lakes on the Qinghai-Tibet and Inner Mongolia Plateaus in northern China. The results showed that high salinity considerably reduced microbial alpha-diversity and niche breadth while increasing within-group similarity among various lake types. High salinity additionally decreased the complexity of microbial networks and enhanced network robustness. The assembly of microbial communities was primarily governed by deterministic processes in high-salinity and eutrophic low-salinity lakes. At decreased salinity, trophic level played a leading role in shaping microbial community structure, and the ecological processes shifted from deterministic processes driven by high salinity to eutrophication-driven deterministic processes. The biomarkers also varied from taxa adapted to high-salinity environments (e.g., Nanoarchaeaeota, Rhodothermia) to those suited for living in freshwater and low-salinity habitats (e.g., Alphaproteobacteria, Actinobacteria). In the case of eutrophication, Actinobacteria, Chloroflexi, and Cyanobacteria became the dominant taxa. Our findings indicate that decreased salinity enables trophic level to play an enhanced role in shaping microbial community structure and assembly in plateau lakes. This study enriches our knowledge about the ecological impacts of salinization and eutrophication in plateau lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The source regions of the Yangtze and Yellow Rivers on the Qinghai-Tibet Plateau are extremely important water resources and ecological functional areas in China, and the ecological environment is fragile and sensitive to climate change. Chromophoric dissolved organic matter (CDOM) is an important component that plays a crucial role in the biogeochemical cycle in aquatic ecosystems. However, knowledge of the distribution characteristics of CDOM in this area is limited. In this study, the optical properties, possible sources of CDOM, and their relationships with environmental variables were investigated in the two regions. The results indicated that the CDOM absorption spectra of these two source regions had a high degree of consistency, and the absorption coefficient a CDOM (355) was small, with a mean of 2.07 ± 1.10 m -1 . Two fluorescence components (C1 and C2) were identified and grouped into the humic-like component with parallel factor analysis (PARAFAC) of fluorescence excitation-emission matrices (EEMs), which exhibited highly similar (excitations/emission)max positions between each pair of components in the two regions. Comprehensive CDOM spectral absorption and fluorescence parameters suggested that CDOM was mainly derived from externally input humus, and the source region of the Yellow River showed stronger allochthonous sources. The dissolved organic carbon (DOC) gradients in the water affected the fluorescence intensity and indicated that the humic-like component was an important component of DOC. Water temperature (WT) and turbidity (Turb) positively affected the concentration of CDOM and the ability to absorb light in the aquatic ecosystems. Due to global warming, the rising temperature may lead to an increase in meltwater inflow in the source area and will also bring more external inputs through the runoff.

For decades, the river health of the Yellow River source region (YRSR) on the Qinghai-Tibetan Plateau has been a focal issue owing to its unique geographic location and ecological functions. This study investigated the ecological status of the headwater streams, the main stem, and the tributaries of the Yellow River in the YRSR using the tolerance values of macroinvertebrates and those related to biotic indices. The macroinvertebrate assemblages of the headwater streams were characterized by lower biodiversity than the tributaries downstream, based on comparisons of taxonomical composition, functional feeding group composition, and the pollution-tolerant capacity of taxa. The headwater streams had a lower ratio (16%) of pollution-sensitive macroinvertebrate taxa than that of the tributaries downstream (30%). The biotic indices (family- and genus-level biotic indices) indicated that the ecological health of the headwater streams was comparably poorer than that of the downstream tributaries. The combined effect of vulnerable natural conditions and increasing human disturbance is likely the main cause of eco-environmental degradation in the Yellow River headwater streams.