1. A new B18 sequence (B*1802) from Asian individuals.
- Author
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Lin L, Tokunaga K, Ishikawa Y, Kashiwase K, Nakajima F, Nishimura M, Kuwata S, Akaza T, Tadokoro K, and Shibata Y
- Subjects
- Amino Acid Sequence, Base Sequence, Codon genetics, DNA Mutational Analysis, Exons genetics, Gene Frequency, HLA-B18 Antigen, Haplotypes genetics, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Thailand, White People genetics, Alleles, Asian People genetics, HLA-B Antigens genetics
- Abstract
A new HLA-B18 allele (B*1802) derived from a Thai individual was sequenced. Comparison of this B18 nucleotide sequence with the published B*1801 sequence indicated that this Asian B18 allele has a nucleotide sequence different from that of B*1801. Three nucleotide changes were observed in exon 3, in which two substitutions at codon 97, AGG in B*1801 to AAT in the B*1802, result in an amino acid change from arginine to asparagine. The residue 97Asn has also been described in some B27 subtypes. A silent mutation was also observed at codon 99, TAC in B*1801 to TAT in the B*1802. This sequence has been reported in many class I alleles published so far. Moreover, 18 HLA-B18-positive samples were examined by the PCR-SSO method using specific probes for B*1801 and B*1802. The results demonstrated that three Asian samples possess B*1802 and share HLA-Cw7, DR12, and DQ7.
- Published
- 1995
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