1. Development of a serotyping ELISA system for Thailand virus infection.
- Author
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Nakamura I, Yoshimatsu K, Lee BH, Okumura M, Taruishi M, Araki K, Kariwa H, Takashima I, and Arikawa J
- Subjects
- Antibodies, Viral immunology, Orthohantavirus isolation & purification, Hantavirus Infections immunology, Humans, Immune Sera immunology, Neutralization Tests, Nucleocapsid Proteins immunology, Thailand, Enzyme-Linked Immunosorbent Assay methods, Orthohantavirus classification, Hantavirus Infections diagnosis, Serotyping
- Abstract
To distinguish Thailand virus infection from infections with other hantaviruses, we established an ELISA serotyping system using a truncated nucleocapsid protein of Thailand virus lacking 49 amino acids at the N-terminus. In evaluations using patient and rodent sera, Thailand virus infection was readily distinguished from Hantaan and Seoul virus infections. Therefore, this ELISA system is an effective alternative to neutralization tests.
- Published
- 2008
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