1. Distribution of human papillomavirus genotypes in suspected women cytological specimens from Tehran, Iran.
- Author
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Tabibzadeh, Alireza, Panahi2., Mahshid, Bouzari, Behnaz, Haghi Ashtiani, Mohammad Taghi, Zamani, Farhad, Arzati, Hadi Teimoori, and Karbalaie Niya, Mohammad Hadi
- Subjects
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PAPILLOMAVIRUS diseases , *GENOTYPES , *PAPILLOMAVIRUSES , *NUCLEIC acids , *MIXED infections , *AGE groups , *RIBAVIRIN - Abstract
Background and Objectives: The human papillomavirus (HPV) is associated with more than 70% of the cervical neoplasm. The current study aims to evaluate the distribution of HPV genotypes in suspected women cytological specimens from Tehran, Iran. Materials and Methods: In the current cross-sectional study, HPV genotype prevalence was investigated in 433 subject women. DNA extraction was performed by High Pure Viral Nucleic Acid kit. A semi-automatically hybriSpot 24™ (HS24) setting was used for HPV typing and data interpreted by hybriSoft™ software according to instructions. Results: Pathologic data showed 181 (41.8%) had non-malignant lesions, 212 (49%) had inflammation and 40 (9.2%) reported LSIL in primary Pap-smear result. HPV was found in 143 (33%) specimens and the most comment high-risk and low-risk HPV types were HPV-16 and -6, respectively. Also, 62 (43%) were co-infected with multiple genotypes includes, 34 (24%) cases had co-infection with two HPV types, 17 (12%) cases had co-infection with three HPV types, 6 (4%) cases had co-infection with four HPV types and 5 (3%) cases had co-infection with five HPV types. There was statistically different domination on high-risk genotype in most of the co-infected samples (p<0.01). Conclusion: Current study indicates that the lesion pathology assessment was significantly associated with the HPV infection (p<0.01). Furthermore, the age group assessment shows that most of the HPV positive cases were 21 to 40 (p<0.01). The HPV infection prevalence in the current study was 33% and the most frequently reported high-risk and low-risk HPV types were 16 and 6, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2022