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Hybrid rice (Oryza sativaL.) cultivars play an important role in rice production due to its high yield potential. Optimum nitrogen (N) rate is necessary to achieve the maximum yield of hybrid. The main objective of this study was to reveal the responses of yield and yield component of hybrid rice ‘Eryou 107’ to different N rates in Nanjing and Taoyuan, a special eco-site. Leaf area index (LAI), dry matter accumulation was also compared. Rice yield showed a quadratic response to N rates at both sites with maximum yields approximately 10 t ha−1at the 195 kg ha−1N rate in Nanjing and maximum yield above 18 t ha−1at the 375 kg ha−1N rate in Taoyuan. Panicle per m2was positively linear related to N rate at both sites in both years, while spikelets per panicle showed a quadratic relation. Larger sink size was the primary contributor to higher yields in Taoyuan compared with Nanjing, and panicle per m2was the main cause. With the increasing N rate, LAI increased linearly and the dry matter accumulation first increased than declined at both sites. Greater effects of N rates on yield and yield components, LAI, and biomass was observed in Taoyuan than Nanjing. Higher LAI, biomass, and larger sink size resulted in the higher yields and more N rate for maximum yields in Taoyuan, compared with Nanjing. [ABSTRACT FROM PUBLISHER]