1. Bacillus lycopersici sp. nov., isolated from a tomato plant (Solanum lycopersicum L.).
- Author
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Lin SY, Hameed A, Liu YC, Wen CZ, Lai WA, Hsu YH, and Young CC
- Subjects
- Bacillus genetics, Bacillus isolation & purification, Base Composition, Cell Wall chemistry, DNA, Bacterial genetics, Diaminopimelic Acid chemistry, Fatty Acids chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan chemistry, Phospholipids chemistry, Plant Stems microbiology, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Taiwan, Vitamin K 2 analogs & derivatives, Vitamin K 2 chemistry, Bacillus classification, Solanum lycopersicum microbiology, Phylogeny
- Abstract
A Gram-stain-positive, rod-shaped, endospore-forming bacterium (designated strain CC-Bw-5(T)) was isolated from chopped tomato stems. The isolate grew at 20-40 °C, pH 6.0-8.0 and tolerated 6% (w/v) NaCl. The most closely related strains in terms of 16S rRNA gene sequence similarity were Bacillus isabeliae (95.3%) and Bacillus oleronius (95.3%). The G+C content of the genomic DNA was 37.2 ± 3.6 mol%. Strain CC-Bw-5(T) was determined to possess C16 : 0, iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 as predominant fatty acids. The polar lipid profile consisted of predominant amounts of diphosphatidylglycerol, and moderate-to-trace amounts of phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified phosphoglycolipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid; menaquinone (MK-7) was the predominant respiratory quinone. According to distinct phylogenetic, phenotypic and chemotaxonomic features, strain CC-Bw-5(T) is proposed to represent a novel species within the genus Bacillus for which the name Bacillus lycopersici sp. nov. is proposed. The type strain is CC-Bw-5(T) ( = BCRC 80623(T) = JCM 19140(T)).
- Published
- 2015
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