1. SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix.
- Author
-
Rippa, Massimo, Castagna, Riccardo, Sagnelli, Domenico, Vestri, Ambra, Borriello, Giorgia, Fusco, Giovanna, Zhou, Jun, Petti, Lucia, and Mukhopadhyay, Sharmila M.
- Subjects
BRUCELLA ,SERS spectroscopy ,COMPLEX matrices ,BRUCELLA abortus ,BIOSENSORS - Abstract
Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (10
4 cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF