1. A highly sensitive 3base™ assay for detecting Streptococcus pyogenes in saliva during controlled human pharyngitis.
- Author
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Indraratna, Anuk D., Mytton, Sacha, Ricafrente, Alison, Millar, Doug, Gorman, Jody, Azzopardi, Kristy I., Frost, Hannah R., Osowicki, Joshua, Steer, Andrew C., Skropeta, Danielle, and Sanderson-Smith, Martina L.
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STREPTOCOCCUS pyogenes , *SALIVA , *PHARYNGITIS , *MORTALITY , *GRAM-positive bacteria - Abstract
Streptococcus pyogenes (Group A Streptococcus; GAS) is a Gram-positive bacterium responsible for substantial human mortality and morbidity. Conventional diagnosis of GAS pharyngitis relies on throat swab culture, a low-throughput, slow, and relatively invasive 'gold standard'. While molecular approaches are becoming increasingly utilized, the potential of saliva as a diagnostic fluid for GAS infection remains largely unexplored. Here, we present a novel, high-throughput, sensitive, and robust speB qPCR assay that reliably detects GAS in saliva using innovative 3base™ technology (Genetic Signatures Limited, Sydney, Australia). The assay has been validated on baseline, acute, and convalescent saliva samples generated from the C ontrolled H uman I nfection for V accination A gainst S treptococcus (CHIVAS-M75) trial, in which healthy adult participants were challenged with emm75 GAS. In these well-defined samples, our high-throughput assay outperforms throat culture and conventional qPCR in saliva respectively, affirming the utility of the 3base™ platform, demonstrating the feasibility of saliva as a diagnostic biofluid, and paving the way for the development of novel non-invasive approaches for the detection of GAS and other oropharyngeal pathogens. [Display omitted] • Group A Streptococcus (GAS) is a human pathogen of global significance. • Diagnosis traditionally relies on throat culture which is slow and low-throughput. • We have developed a novel saliva-based PCR using 3base™ technology. • It has been validated among a cohort of patients from a human challenge model. • The saliva PCR exhibits greater sensitivity than throat culture. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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