1. Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname.
- Author
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van Boheemen S, Tas A, Anvar SY, van Grootveld R, Albulescu IC, Bauer MP, Feltkamp MC, Bredenbeek PJ, and van Hemert MJ
- Subjects
- Adult, Americas epidemiology, Animals, Chlorocebus aethiops, Female, Genome, Viral genetics, Humans, Phylogeny, Suriname, Travel, Vero Cells, Viral Envelope Proteins genetics, Viral Nonstructural Proteins genetics, Zika Virus classification, Zika Virus physiology, Zika Virus Infection epidemiology, Zika Virus Infection virology, Quasispecies, Zika Virus genetics, Zika Virus Infection diagnosis
- Abstract
The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5' and 3' untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10
-4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate.- Published
- 2017
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