1. Profile and persistence of the virus-specific neutralizing humoral immune response in human survivors of Sudan ebolavirus (Gulu).
- Author
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Sobarzo A, Groseth A, Dolnik O, Becker S, Lutwama JJ, Perelman E, Yavelsky V, Muhammad M, Kuehne AI, Marks RS, Dye JM, and Lobel L
- Subjects
- Animals, Antibodies, Neutralizing blood, Antibodies, Viral blood, Antigens, Viral blood, Cell Line, Chlorocebus aethiops, HEK293 Cells, Hemorrhagic Fever, Ebola blood, Hemorrhagic Fever, Ebola virology, Humans, Immunity, Humoral immunology, Immunoglobulin A immunology, Neutralization Tests methods, Sudan, Survivors, Vero Cells, Viral Envelope Proteins immunology, Antibodies, Neutralizing immunology, Antibodies, Viral immunology, Antigens, Viral immunology, Ebolavirus immunology, Hemorrhagic Fever, Ebola immunology
- Abstract
To better understand humoral immunity following ebolavirus infection, a serological study of the humoral immune response against the individual viral proteins of Sudan ebolavirus (Gulu) in human survivors was performed. An enzyme-linked immunosorbent assay specific for full-length recombinant viral proteins NP, VP30, VP40, and GP1-649 (GP lacking the transmembrane domain) of Sudan ebolavirus (Gulu) was used as well as a plaque reduction neutralization test. Serum samples from human survivors, which were collected up to 10 years following recovery, were screened and analyzed. Results demonstrate that samples obtained 10 years following infection contain virus-specific antibodies that can neutralize virus. Neutralization correlates well with immunoreactivity against the viral proteins NP, VP30, and GP1-649. Sera from individuals who died or those with no documented infection but immunoreactive to ebolavirus did not neutralize. This work provides insight into the duration, profile of immunoreactivity, and neutralization capacity of the humoral immune response in ebolavirus survivors.
- Published
- 2013
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