1. Genetic Changes at the Glycoprotein Editing Site Associated With Serial Passage of Sudan Virus.
- Author
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Alfson KJ, Avena LE, Beadles MW, Menzie H, Patterson JL, Carrion R Jr, and Griffiths A
- Subjects
- Animals, Chlorocebus aethiops, Genome, Viral genetics, Haplorhini, Serial Passage methods, Sudan, Vero Cells virology, Viral Load methods, Virulence genetics, Ebolavirus genetics, Ebolavirus pathogenicity, Glycoproteins genetics, Hemorrhagic Fever, Ebola virology, RNA Editing genetics
- Abstract
Sudan virus (SUDV), like the closely related Ebola virus (EBOV), is a filovirus that causes severe hemorrhagic disease. They both contain an RNA editing site in the glycoprotein gene that controls expression of soluble and full-length protein. We tested the consequences of cell culture passage on the genome sequence at the SUDV editing site locus and determined whether this affected virulence. Passage resulted in expansion of the SUDV editing site, similar to that observed with EBOV. We compared viruses possessing either the wild-type or expanded editing site, using a nonhuman primate model of disease. Despite differences in virus serum titer at one time point, there were no significant differences in time to death or any other measured parameter. These data imply that changes at this locus were not important for SUDV lethality., (© The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2015
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