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Within the framework of the CaixaProinfancia programme, networks have been developed in 18 regions of Spain which promote intersectoral cooperation to combat the social and educational exclusion of children. Their emerging nature and simultaneous development have enabled a comparative study to be made of their organisation, the supporting and inhibiting factors, and the perception of the results. The conclusions stress the importance of leadership which takes into account the principles of transversality and horizontality, as well as properly focusing the socioeducational action and implementing it within a relatively short period of time. Some networks evolve in their approach, going from attending to the participants' needs to more global approaches to socioeducational governance.

Background: Prone position (PP) has been widely used in the COVID-19 pandemic for ARDS management. However, the optimal length of a PP session is still controversial. This study aimed to evaluate the effects of prolonged versus standard PP duration in subjects with ARDS due to COVID-19. Methods: This was a single-center, randomized controlled, parallel, and open pilot trial including adult subjects diagnosed with severe ARDS due to COVID-19 receiving invasive mechanical ventilation that met criteria for PP between March-September 2021. Subjects were randomized to the intervention group of prolonged PP (48 h) versus the standard of care PP (-16 h). The primary outcome variable for the trial was ventilator-free days (VFDs) to day 28. Results: We enrolled 60 subjects. VFDs were not significantly different in the standard PP group (18 [interquartile range [IQR] 0-23] VFDs vs 7.5 [IQR 0-19.0] VFDs; difference, -10.5 (95% CI -3.5 to 19.0, P = .08). Prolonged PP was associated with longer time to successful extubation in survivors (13.00 [IQR 8.75-26.00] d vs 8.00 [IQR 5.00-10.25] d; difference, 5 [95% CI 0-15], P = .001). Prolonged PP was also significantly associated with longer ICU stay (18.5 [IQR 11.8-25.3] d vs 11.50 [IQR 7.75-25.00] d, P = .050) and extended administration of neuromuscular blockers (12.50 [IQR 5.75-20.00] d vs 5.0 [IQR 2.0-14.5] d, P = .005). Prolonged PP was associated with significant muscular impairment according to lower Medical Research Council values (59.6 [IQR 59.1-60.0] vs 56.5 [IQR 54.1-58.9], P = .02). Conclusions: Among subjects with severe ARDS due to COVID-19, there was no difference in 28-d VFDs between prolonged and standard PP strategy. However, prolonged PP was associated with a longer ICU stay, increased use of neuromuscular blockers, and greater muscular impairment. This suggests that prolonged PP is not superior to the current recommended standard of care. [ABSTRACT FROM AUTHOR]

Undergraduate courses in electrical machines often include an introduction to their magnetic circuits and to the various magnetic materials used in their construction and their properties. The students must learn to be able to recognize and compare the permeability, saturation, and losses of these magnetic materials, relate each material to its specific properties, and understand the impact of these properties on the major performance metrics of electrical machines. This paper describes a new test equipment setup and lab guide that helps students achieve these learning goals. The test equipment consists of two transformers of grain-oriented and non-grain-oriented electrical steel, transducers, a data acquisition (DAQ) board and a PC-based virtual instrument. The virtual instrument shows voltage, current, and core flux time waveforms, the rms voltage versus current curves and, most importantly, the lamination material magnetic cycle. Students' laboratory work was organized into a series of experiments that guide their achievement of these magnetic materials-related abilities. Pre- and post-lab exams assessed student learning, which was shown to have increased significantly. Students' opinions of the relevance, usefulness, and motivational effect of the laboratory were also positive.

First-year university students exhibit considerably different levels of proficiency, often lacking the necessary prerequisites to attend certain courses. This is especially true for mathematics courses. While motivation alone plays a role, by far the most significant challenge consists in harmonising the different (and often incompatible) backgrounds of the students. In response, universities have made various attempts to tackle this issue. Many institutions now offer intensive courses prior to academic entry or even complete introductory courses occupying the whole first term, whether in a classroom or (partially) online. Massive Open Online Courses (MOOCs), while relatively unknown until a few months ago, have brought about a real educational revolution. The MOOC is an educational model that brings online learning content to any person willing to participate. At Pompeu Fabra University (UPF) we were intrigued by this new educational paradigm and wondered whether it could be useful in filling the gap between high school and university. Therefore, we recently designed and implemented such a course: while remaining available to anyone, the course specifically targets our prospective students who in turn should tackle first-year linear algebra having acquired an adequate background. In this paper we describe the first MOOC launched by UPF, "Decoding Algebra". We provide an overview of its design, the technological means we used, our students' profile and the lessons from this experience. More importantly, by focusing on our incoming students, we examine whether MOOCs can be a good approach to level out the differences among first-year university students.

The aim of this study was to describe and analyze the involvement showed by 190 students who participated in an introduction to Information and Communication Technologies (ICT) project, in public schools from Spain. By means of an Internet browsing test, access and information selection processes were afforded. The purpose of the study was to explore whether students who usually worked with ICT in their classrooms, had better knowledge and management with computing tools, and if they are better prepared to research and select information. Results show that students of elementary and secondary education, who have a greater contact with ICT, but not specifically introduced to searching for information on the Internet, know how to use the technology. All these students show better knowledge and control of the computer as well as presenting better Web literacy skills. Although they found, organized, used and assessed the information better their literacy in Web information is missing key skills, especially in analyzing, assessing and summarizing information.

Evidence is growing that two modalities of computer-based exposure therapies--virtual reality and computer-aided psychotherapy--are effective in treating anxiety disorders, including fear of flying. However, they have not yet been directly compared. The aim of this study was to analyze the efficacy of three computer-based exposure treatments for fear of flying: virtual reality exposure therapy (VRET), computer-aided exposure with a therapist's (CAE-T) assistance throughout exposure sessions, and self-administered computer-aided exposure (CAE-SA). A total of 60 participants with flying phobia were randomly assigned to VRET, CAE-T, or CAE-SA. Results indicate that the three interventions were effective in reducing fear of flying at posttreatment and at 1-year follow-up; furthermore, there were no significant differences between them in any of the outcome measure. Large within-group effect sizes were found for all three treatment conditions at both posttreatment and at follow-up. The results suggest that therapist involvement might be minimized during computer-based treatments and that CAE can be as effective as VRET in reducing fear of flying. (Contains 3 tables.)

Pneumococcal conjugate vaccine (PCV13) has been recently added to the vaccine recommendations for immunosuppressed adults (ISP). We conducted a multicenter observational prospective study aimed to assess the evolving epidemiology of invasive pneumococcal disease (IPD) in adults, with especial focus on ISP. All IPD cases admitted from 1999 to 2014 were included. ISP was defined as patients on current cancer chemotherapy, immunosuppressive therapy for autoimmune disease, biological therapy, chronic systemic steroid use, hemodialysis, neutropenia or HIV infection. A total of 799 IPD episodes were analyzed, 198 were considered ISP. IPD incidence decreased from 20 to 8/100,000-population year (p < 0.004) over the study period. No changes in mortality were observed. Penicillin resistance experienced a significant decline. In 694 episodes the serotype was known. Global vaccine coverage considering the whole study period, was for PCV7 21.6% vs. 28.8% in general and in immunosuppressed population (p = 0.04) and for PCV13 64.5% and 56.6% respectively (p = 0.05). The proportion of IPD isolates included in PCV7 and PCV13 significantly decreased over time. A reduction in the incidence of IPD in adults was seen late after the vaccine licensure, both in general population and in ISP. Coverage of PCV13 vaccine might be suboptimal for ISP in the coming years. [ABSTRACT FROM AUTHOR]

Climate change (CC) may have substantial impacts on the distribution of current tourist flows by changing patterns of seasonal volumes of tourist demand. Such impacts are of potential importance for the Spanish coastal tourism destinations. In a context where the implementation of adaptation measures becomes relevant to counteract the expected CC-induced travel market share losses, this paper examines the role of preference analysis in the design of CC adaptation policies. Using data from the 2005 Familitur Survey on summer domestic tourist flows, a destination choice model is implemented to: 1) highlight the role of temperature and its relationship with other destination-specific attributes, 2) estimate CC-induced changes in travel market shares, and 3) compute the economic value tourists assign to a set of destination assets as a way to better permit the recovery the expected market share losses by regional tourism authorities. [ABSTRACT FROM AUTHOR]

BACKGROUND: Electrical impedance tomography measures changes in lung impedance, which are mainly related to changes in lung volume. We used electrical impedance tomography to investigate the effects of high-flow nasal cannula (HFNC) and body position on global and regional end-expiratory lung impedance variation (ΔEELI). METHODS: Prospective study with 20 healthy adults. Two periods were defined: the first in supine position and the second in prone position. Each period was divided into 3 phases. In the first and the third phases the subjects were breathing ambient air, and in the second HFNC was implemented. Four regions of interest were defined: 2 ventral and 2 dorsal. For each respiratory cycle, global and regional ΔEELI were measured by electrical impedance tomography and were expressed as a function of the tidal variation of the first stable respiratory cycle (units). RESULTS: HFNC increased global EELI by 1.26 units (95% CI 1.20 -1.31, P < .001) in supine position, and by 0.87 units (95% CI 0.82- 0.91, P < .001) in prone position. The distribution of ΔEELI was homogeneous in prone position, with no difference between ventral and dorsal lung regions (_0.01 units, 95% CI _0.01 to 0, P= .18), while in supine position a significant difference was found (0.22 units, 95% CI 0.21- 0.23, P < .001) with increased EELI in ventral areas. CONCLUSIONS: HFNC increased global EELI in our population, regardless of body position, suggesting an increase in functional residual capacity. Prone positioning was related to a more homogeneous distribution of ΔEELI, while in supine position _EELI was higher in the ventral lung regions. [ABSTRACT FROM AUTHOR]

Abstract: Epidemiological surveillance of Clostridium difficile infection has gained importance in recent years as a result of the rapid spread of epidemic strains, including hypervirulent strains and strains with reduced susceptibility to antimicrobials. The molecular epidemiology and antimicrobial susceptibility of C. difficile in the reference hospital of the Balearic Islands (Spain) is reported in this study. One hundred isolates of toxigenic C. difficile from different patients were selected using rapid dual EIA screening test. All isolates were characterized through toxin profile, PCR ribotyping and, in addition, multi-locus sequence typing (MLST) was performed on fifty selected strains. MICs to metronidazole, vancomycin, erythromycin and moxifloxacin were also determined. A total of 43 different ribotypes were distinguished, with higher prevalence of ribotype 014 (34%). Twenty one per cent of the isolates expressed binary toxin and it is noteworthy that 62% of these were identified as the hypervirulent ribotype 078, the second most prevalent ribotype found in our hospital (13%). A total of 20 different sequence types (STs) were found, including a new described allele and ST. MLST data showed a clear concordance between some ribotypes and STs, mainly represented by ribotype 014/ST-2, ribotype 078/ST-11 and ribotype 001/ST-3. Phylogenetic analysis also revealed that most of the isolates were genetically related, forming a large clonal complex. Finally, ribotypes 078 (ST-11) and 001 (ST-3) were associated with higher resistance to erythromycin and to erythromycin and moxifloxacin, respectively. All these data suggest that the combination of ribotyping and MLST is a good tool for the surveillance of the changing epidemiology of C. difficile. A wide dissemination of clones has been observed in our setting, ribotype 014 (ST-2) being the most prevalent followed by the hypervirulent ribotype 078 (ST-11) and ribotype 001 (ST-3), their spread in our setting probably influenced by their higher resistance. [Copyright &y& Elsevier]

The study describes the periodic use of cervical and breast cancer screening by women residing in Spain, according to their country of origin, and analyzes whether the observed associations are modified by social class. A cross-sectional design was used, with the study population consisting of women residing in Spain in 2006, ages 25-65 years (N = 10,093) and 40-69 years (N = 6,674) in the cervical and breast cancer screening groups, respectively. The information source was Spain's National Health Survey of 2006. The dependent variables were: undergoing periodic cervical cancer screening (every 5 years or less) and breast cancer screening (every 2 years or less). The independent variables were: country of origin, social class, health care coverage, cohabitation, and age. A descriptive analysis was carried out, and robust Poisson regression models were fitted. Women from low-income countries underwent fewer periodic screening exams for cervical cancer and breast cancer. Independent of country of origin, women from the manual classes underwent fewer screening exams than those from the non-manual classes. In the 50-69 years age group, it was mainly women from the manual classes from low-income countries who underwent fewer periodic mammograms. Having only public health care coverage and not cohabiting with a partner were also associated with lower prevalences of use. [ABSTRACT FROM AUTHOR]

Purpose – The purpose of this paper is to determine the level of knowledge, among university professors, about copyright problems related to e-learning activities. Design/methodology/approach – An online questionnaire was used to survey professors teaching subjects online. Findings – There is a considerable lack of knowledge on the part of professors regarding copyright issues, both in general terms and in specific matters related to digital learning. Strong discrepancies are found between the text of the law and the notion actually held by professors as to how legislators have formulated the law. Originality/value – This information may be useful for professors themselves as well as for persons managing the e-learning platforms of universities. It demonstrates that the law was reformed without any discussion with those actually engaged in online teaching. [ABSTRACT FROM AUTHOR]

A serosurvey on porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Aujeszky’s disease virus gE protein (ADV gE), porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2) was carried out in Spanish pig herds. The serosurvey consisted of two studies. First, a retrospective study assessed the proportion of seropositive boar, sow and fattening pig herds and their seroprevalences to PRRSV, SIV, ADV gE and PPV from 2003 to 2005 and to PCV2 from 2000 to 2005. Such information was obtained from routine serologic analyses from two veterinary diagnostic laboratory services. Second, a cross-sectional study in sow and fattening pig herds from 44 farms (without vaccination interferences on serologic analyses) was performed to provide information on seroprevalences and co-seropositivity to PRRSV, SIV, ADV gE and PCV2 (PPV was excluded because of widespread vaccination) and to elucidate their relationships with farm characteristics, management and productive parameters. Similar seroprevalences were observed in both studies, although some variations were obtained, probably because of vaccination schedules, number of tested sera, sampling age and regional variations. Percentage of PRRSV and SIV seropositive herds was over 85% for sows, around 80% for fatteners and around 50% for boar studs. The proportion of ADV gE seropositive sow herds decreased from 41% to 30% between 2003 and 2005, whereas such decrease was from 41% to 33% in fattening pig herds and from 13% to 4% in boar studs PCV2 antibodies were widespread as well as those against PPV; in the latter case, if antibodies were elicited by infection and/or vaccination was not assessed. Concurrent presence of PCV2, PRRSV and SIV antibodies was found in 89% and 66% sow and fattening herds, respectively. No statistical associations were obtained between seroprevalences or co-seropositivity and farm characteristics, management or productive parameters. [ABSTRACT FROM AUTHOR]

The discourse of the stereotype of the usurers of the Jews in the 13th century has been constructed without prior heuristic work on the relevant legislation. This article enumerates the constitutions and statutes dictated by James I regarding the usurers, and the usurers of the Jews, between 1228 and 1251, from shortly before to shortly after the conquest of the kingdoms of Majorca and Valencia. Surprisingly, there are fourteen of these, not included in any directory. The statutes are described in chronological order, the date and the territorial scope are established, along with the best copies and the prints that have made them accessible. Numerous confusions and defective interpretations are also corrected, and citations from non-existent statutes are reported. [ABSTRACT FROM AUTHOR]

The article discusses the results of a study on isolation rate, serovars and toxin genotypes of nicotinamide adenine dinucleotide-independent Actinobacillus pleuropneumoniae among pigs with pleuropneumonia in Spain. Using microbiology and species-specific polymerase chain reaction (PCR), 127 biovar 2 isolates were identified from 502 A. pleuropneumoniae. About 4.7% of the identified isolates belong to serovar 2 and 68.5% for serovar 11. It also revealed that all bovar 2 isolates had genes of the apxII operon which encodes ApxII exotoxin.

Abstract: Tourism statistics point towards increased tourist mobility in holiday destinations, with a subsequent rise in associated externalities, above all those related with the use of hire cars. This paper therefore suggests that a fixed-rate tax should be levied on vehicle hire in order to reduce congestion externalities and thus achieve more efficient traffic levels. With this purpose in mind, count data models were used to assess alternative ways of modelling the demand function for hire cars in Mallorca (Spain) so that the corrective potential of the proposed tax could then be analysed. Although the inelasticity that was observed might indicate a low short-run capacity to internalise costs, it also represents a high potential for raising revenue that could be used to fund alternative means of group transport. In the mid-run, this would lead to increased elasticity and, in consequence, to better internalising effectiveness. [Copyright &y& Elsevier]

The article presents a study that evaluates the socioeconomic effects of possible climate change on shrublands in the Mediterranean region. It aims to estimate the trade-offs for changes in plant cover, fire risk and soil erosion, and the willingness of citizens in Catalonia, Spain to pay for management programs or new policies that will help mitigate the intensity of such changes. The findings suggest that the welfare of individual Catalans is expected to decline with the changes in shrublands, and that Catalans are most concerned about soil erosion, followed by fire risk and plant cover.

During the last few decades, tourism has undergone rapid growth. However, it seems clear that this growth is not exempt from certain costs. Economists’ attention is increasingly directed at the economic, social, cultural and environmental repercussions of tourism, regarded as externalities. Focusing on the aforementioned repercussions, the aim of this study is to confirm both the existence of external environmental costs and the need to internalise them, with the aid of appropriate economic instruments. In this respect, an outline is given of the recent proposal by the Government of the Autonomous Community of the Balearic Islands to introduce a tourist tax on visitors, presented to public opinion in the form of an environmental tax, and a critical review is made of the proposal, in the light of a number of criteria regarded as relevant in the description, design and implementation of these taxes. [Copyright &y& Elsevier]

Valuing environmental goods through benefit transfer is based on information from the set of past empirical studies of similar or identical sites. This paper proposes a Bayesian approach to model prior information on mean consumer surplus. The prior distribution is defined as a convex combination of the results from past empirical studies. An advantage of the Bayesian approach is that the prior distribution could be combined with on-site sample information in order to improve predictions on the benefits of the policy site. The model is applied to data from national parks in Spain. The results show that the benefits of policy sites are more sensitive to the prior information for smaller sample sizes. This implies that the prior distribution can be seen as a substitute for on-site sample information. [ABSTRACT FROM AUTHOR]

This paper investigates the effects of varying attribute ranges to find evidence of anomalous preference formation or, by contrast, of apparent differences in preferences linked to unexplained error variance. A choice experiment study aimed at estimating tourists' preferences for alternative climate change adaptation policies in Mallorca, Spain, has provided the empirical framework varying the ranges of values for all non-cost attributes between two split-sample treatments. Our results suggest that the greater difficulty associated to wider ranges increases the variance with which respondents make their choices which are, in this case, more random. However, once scale differences between treatments are controlled for, the varying attribute range has no effect on utility parameters (preferences) and marginal willingness to pay. • Attribute range effects disappear once scale differences are controlled for. • Trade-offs between alternatives become harder to make with wider attribute ranges. • Wider attribute ranges increases the variance with which respondents make choices. [ABSTRACT FROM AUTHOR]

of the present cross sectional study were to analyze the offer of subjects with communication skills in the nursing degree in Spain and to describe the distribution of these subjects. Documentary, systematic and independent search of web pages from Spanish universities was carried out to collect the following variables: subjects with communication skills, course in which the subject is taught, type of teaching (exclusive or combined), type of subject (compulsory or optional) and number of credits on communication skills. Although an average of 3.6 subjects per center was offered, most of the subjects were combined and with little communication content load. In one third of the centers, the offer was below 2.3 credits. Only 1 in 6 centers had exclusive communication skills subjects, and a quarter of them were optional. The teaching load of communication contents was highest in optional subjects. The offer of communication skills contents in Spanish Nursing Schools was scarce and very heterogeneous between centers and between courses in a center, with excessive presence of combined and optional subjects. Our results may be useful when developing the teaching guides for subjects with communication skills, as well as when defining communication competencies in the different Nursing Schools. • The offer of communication skills contents in Spanish Nursing Schools was scarce and heterogeneous. • Most of the communication subjects were combined, with contents other than communication. • Most of the communication subjects were optional. • Our study showed the need to improve contents of communication skills in the nursing degree in Spain. [ABSTRACT FROM AUTHOR]

Background: Depression is the most prevalent cause of illness-induced disability worldwide. Face-to-face psychotherapeutic interventions for depression can be challenging, so there is a need for other alternatives that allow these interventions to be offered. One feasible alternative is Internet-based psychological interventions. This is the first randomized controlled trial (RCT) on the effectiveness of an Internet-based intervention on depression in primary health care in Spain.Objective: Our aim was to compare the effectiveness of a low-intensity therapist-guided (LITG) Internet-based program and a completely self-guided (CSG) Internet-based program with improved treatment as usual (iTAU) care for depression.Methods: Multicenter, three-arm, parallel, RCT design, carried out between November 2012 and January 2014, with a follow-up of 15 months. In total, 296 adults from primary care settings in four Spanish regions, with mild or moderate major depression, were randomized to LITG (n=96), CSG (n=98), or iTAU (n=102). Research completers at follow-up were 63.5%. The intervention was Smiling is Fun, an Internet program based on cognitive behavioral therapy. All patients received iTAU by their general practitioners. Moreover, LITG received Smiling is Fun and the possibility of psychotherapeutic support on request by email, whereas CSG received only Smiling is Fun. The main outcome was the Beck Depression Inventory-II at 3 months from baseline. Mixed-effects multilevel analysis for repeated measures were undertaken.Results: There was no benefit for either CSG [(B coefficient=-1.15; P=.444)] or LITG [(B=-0.71; P=.634)] compared to iTAU, at 3 months. There were differences at 6 months [iTAU vs CSG (B=-4.22; P=.007); iTAU vs LITG (B=-4.34; P=.005)] and 15 months [iTAU vs CSG (B=-5.10; P=.001); iTAU vs LITG (B=-4.62; P=.002)]. There were no differences between CSG and LITG at any time. Adjusted and intention-to-treat models confirmed these findings.Conclusions: An Internet-based intervention for depression combined with iTAU conferred a benefit over iTAU alone in the Spanish primary health care system.Trial Registration: Clinicaltrials.gov NCT01611818; https://register.clinicaltrials.gov/prs/app/action/SelectProtocol? selectaction=Edit&uid=U0001NPQ&ts=2&cx=gctdh2&sid=S0003KJ6 (Archived by WebCite at http://www.webcitation.org/6jbsUvUDz). [ABSTRACT FROM AUTHOR]

Introduction: Identifying polymorphisms in the dihydropyrimidine dehydrogenase (DPYD) gene is gaining importance to be able to predict fluoropyrimidine-associated toxicity. The aim of this project was to describe the frequency of the DPYD variants DPYD*2A (rs3918290); c.1679T>G (rs55886062); c.2846A>T (rs67376798) and c.1129-5923C>G (rs75017182; HapB3) in the Spanish oncological patients., Material and Methods: Cross-sectional and multicentric study (PhotoDPYD study) conducted in hospitals located in Spain designed to register the frequency of the most relevant DPYD genetic variants in oncological patients. All oncological patients with DPYD genotype were recruited in the participant hospitals. The measures determined where the presence or not of the 4 DPYD previously described variants., Results: Blood samples from 8054 patients with cancer from 40 different hospitals were used to determine the prevalence of the 4 variants located in the DPYD gene. The frequency of carriers of one defective DPYD variant was 4.9%. The most frequently identified variant was c.1129-5923C>G (rs75017182) (HapB3), in 2.9%, followed by c.2846A>T (rs67376798) in 1.4%, c.1905 + 1G>A (rs3918290, DPYD*2A) in 0.7% and c.1679T>G (rs55886062) in 0.2% of the patients. Only 7 patients (0.08%) were carrying the c.1129-5923C>G (rs75017182) (HapB3) variant, 3 (0.04%) the c.1905 + 1G>A (rs3918290, DPYD*2A) and one (0.01%) the DPYD c.2846A>T (rs67376798, p.D949V) variant in homozygosis. Moreover, 0.07% were compound heterozygous patients, 3 carrying the DPYD variants DPYD*2A + c.2846A>T, 2 the DPYD c.1129-5923C>G + c.2846A>T and one the DPYD*2A + c.1129-5923C>G variants., Conclusions: Our results demonstrate the relatively high frequency of DPYD genetic variants in the Spanish patient with cancer population, which highlights the relevance of their determination before initiating a fluoropirimidine-containing regimen., (© The Author(s) 2023. Published by Oxford University Press.)

The term neuromuscular disorder (NMD) includes many genetic and acquired diseases and differential diagnosis can be challenging. Next-generation sequencing (NGS) is especially useful in this setting given the large number of possible candidate genes, the clinical, pathological, and genetic heterogeneity, the absence of an established genotype-phenotype correlation, and the exceptionally large size of some causative genes such as TTN , NEB and RYR1. We evaluated the diagnostic value of a custom targeted next-generation sequencing gene panel to study the mutational spectrum of a subset of NMD patients in Spain. In an NMD cohort of 207 patients with congenital myopathies, distal myopathies, congenital and adult-onset muscular dystrophies, and congenital myasthenic syndromes, we detected causative mutations in 102 patients (49.3%), involving 42 NMD-related genes. The most common causative genes, TTN and RYR1, accounted for almost 30% of cases. Thirty-two of the 207 patients (15.4%) carried variants of uncertain significance or had an unidentified second mutation to explain the genetic cause of the disease. In the remaining 73 patients (35.3%), no candidate variant was identified. In combination with patients' clinical and myopathological data, the custom gene panel designed in our lab proved to be a powerful tool to diagnose patients with myopathies, muscular dystrophies and congenital myasthenic syndromes. Targeted NGS approaches enable a rapid and cost-effective analysis of NMD- related genes, offering reliable results in a short time and relegating invasive techniques to a second tier.

The present study focused on Mycoplasma hyopneumoniae (M. hyopneumoniae) detection by nPCR in nasal swabs of 507 suckling pigs. These animals came from 69 sows (from 1 to 8 parity number) of a farrow-to-finish herd with Enzootic Pneumonia (EP) problems at finishing stages. At 1 and 3 weeks of age (still in the farrowing units), nasal swabs and blood samples were taken from all piglets. Moreover, from these 507 animals, 37 randomly selected pigs were necropsied at 3 weeks of age. From those necropsied pigs, M. hyopneumoniae presence was tested in bronchial and tonsillar swabs. At 1 week post-farrowing, blood samples from sows were collected and used to detect M. hyopneumoniae antibodies. From the 69 analysed sows, 19 (27.5%) were seropositive. Global percentage of pigs with M. hyopneumoniae detection in nasal swabs at 1 and 3 weeks of age was 1.5% (8 out of 507) and 3.8% (19 out of 507), respectively. From these nPCR positive pigs, 89% (24 out of 27) were seronegative and 11% were seropositive. From necropsied animals, the pathogen DNA was detected in two pigs at bronchus level and in another pig at tonsil. In this study, sow parity was not statistically related with sow seropositivity and piglet colonization. These results confirm that M. hyopneumoniae infection may be detected not only in nasal cavities of naturally infected suckling piglets but also at their low respiratory tract airways. Our results suggest that M. hyopneumoniae detection in lower and upper respiratory tract could be an indicator that respiratory problems associated to EP may start relatively early in the production system. In consequence, sow-to-piglet and/or piglet-to piglet transmission in farrowing barns should not be underestimated.

This paper reports on a serological and virological survey for swine influenza virus (SIV) in densely populated pig areas in Spain. The survey was undertaken to examine whether the H1N2 SIV subtype circulates in pigs in these areas, as in other European regions. Six hundred sow sera from 100 unvaccinated breeding herds across Northern and Eastern Spain were examined using haemagglutination inhibition (HI) tests against H1N1, H3N2 and H1N2 SIV subtypes. Additionally, 225 lung samples from pigs with respiratory problems were examined for the presence of SIV by virus isolation in embryonated chicken eggs and by a commercial membrane immunoassay. The virus isolates were further identified by HI and RT-PCR followed by partial cDNA sequencing. The HI test on sera revealed the presence of antibodies against at least one of the SIV subtypes in 83% of the herds and in 76.3% of the animals studied. Of the 600 sow sera tested, 109 (18.2%), 60 (10%) and 41 (6.8%) had SIV antibodies to subtype H1N2 alone, H3N2 alone and H1N1 alone, respectively. Twelve H3N2 viruses, 9 H1N1 viruses and 1 H1N2 virus were isolated from the lungs of pigs with respiratory problems. The analysis of a 436 nucleotide sequence of the neuraminidase gene from the H1N2 strain isolated further confirmed its identity. Demonstrably, swine influenza is still endemic in the studied swine population and a new subtype, the H1N2, may be becoming established and involved in clinical outbreaks of the disease in Spain.

The objective of the present study was to determine the presence of recognised abortifacient viruses such as porcine reproductive and respiratory virus (PRRSV), Aujeszky's disease virus (ADV), porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2), in tissues from aborted fetuses and stillborn neonates in cases of late reproductive failure in swine. A total of 293 specimens (fetuses aborted in the last third of gestation and stillborn piglets) from 100 different cases of late-term abortions and premature farrowing from 15 different Spanish provinces were studied. PRRSV was detected in 9/100 cases by RT-PCR. Only 1/100 cases analysed (corresponding to a late-term aborted fetus with a negative PRRSV RT-PCR result) was positive for PCV2 by PCR. Neither ADV (monitored by viral isolation plus antigen detection) nor PPV (monitored by ELISA antigen capture test) infection was identified. The results suggest that PRRSV is one of the most important infectious agents, if not the most relevant one, associated with fetal infection leading to abortion or premature farrowing in Spain. Moreover, other viral pathogens such as ADV, PPV and PCV2 seem to have a minor impact on reproductive disease.

From 1993 to 1997, 327 strains of Haemophilus parasuis were isolated from spanish swine in our Diagnostic Laboratory and 174 strains (53.2%) were serotyped. Four serotypes, sv. 5 (18.4%), sv 4 (16%), sv. 2 (9.2%) and sv. 13 (8%) were the most frequently isolated and 29.3% of the studied strains were classified as non typable. The results obtained indicate that the distribution of the serotypes in Spain is very similar to that found by other researchers in Germany, Australia, Canada and alike to that found in the United States.

A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1).

Seventy-one isolates of Actinobacillus pleuropneumoniae isolated from the lungs of pigs in outbreaks of pleuropneumonia in Spain were serotyped by indirect haemagglutination. Serotype 4 (42.2 per cent), serotype 7 (22.5 per cent) and serotype 2 (12.8 per cent) were predominant, whereas serotypes 1, 3, 6, 8, 9, 12 and untypable isolates were present only in small numbers. Serotypes 1, 2, 4 and 7 originated mainly from cases of acute pleuropneumonia, whereas serotypes 3, 6, 8, 9 and 12 were associated with chronically infected herds. The susceptibility of the isolates to 20 antimicrobial agents was determined by agar disc diffusion. Most were susceptible to cefuroxime, cefaclor, cefazolin, kanamycin, tobramycin, gentamicin, oxolinic acid, ciprofloxacin, enoxacin, thiamphenicol, colistin and trimethoprim/sulphamethoxazole. Marked resistance was found with amoxicillin, ticarcillin, oxytetracycline, doxycycline and metronidazole. Rifampicin, fosfomycin and tiamulin were the agents most effective against the isolates tested.