10 results on '"Pumarola, M."'
Search Results
2. Molecular profiling and comparison of field transmissible spongiform encephalopathy cases diagnosed in Catalunya.
- Author
-
Vidal, E., Márquez, M., Costa, C., Tortosa, R., Domènech, A., Serafín, A., and Pumarola, M.
- Subjects
- *
MOLECULAR diagnosis , *PROTEINASES , *PRIONS , *BOVINE spongiform encephalopathy diagnosis , *DIAGNOSIS of chronic wasting disease , *SCRAPIE diagnosis - Abstract
Molecular profiling of the proteinase K resistant prion protein (PrPres) is a technique that has been applied to the characterisation of transmissible spongiform encephalopathy (TSE) strains. An interesting example of the application of this technique is the ability to differentiate, at the experimental level, between bovine spongiform encephalopathy (BSE) and scrapie infection in sheep, and to distinguish between classical and atypical BSE and scrapie cases. Twenty-six BSE cases and two scrapie cases from an active TSE surveillance program and diagnosed at the PRIOCAT, TSE Reference Laboratory (Centre de Recerca en Sanitat Animal, Universitat Autònoma de Barcelona, Catalunya, Spain) were examined by Western blotting. Molecular profiling was achieved by comparing the glycosylation profile, deglycosylated PrP molecular weight and 6H4/P4 monoclonal antibody binding ratio. The results obtained during the characterisation of these field cases indicated an absence of atypical BSE cases in Catalunya. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
3. Immunohistochemical evaluation of tissue factor, fibrin/fibrinogen and D-dimers in canine gliomas.
- Author
-
de la Fuente C, Pumarola M, Blasco E, Fernández F, Viu J, and Añor S
- Subjects
- Animals, Astrocytoma etiology, Astrocytoma metabolism, Astrocytoma veterinary, Biomarkers, Dog Diseases etiology, Dogs, Fibrin metabolism, Fibrin Fibrinogen Degradation Products genetics, Fibrin Fibrinogen Degradation Products metabolism, Fibrinogen metabolism, Glioma etiology, Glioma metabolism, Immunohistochemistry veterinary, Oligodendroglioma etiology, Oligodendroglioma metabolism, Oligodendroglioma veterinary, Spain, Thromboplastin metabolism, Thrombosis etiology, Thrombosis metabolism, Dog Diseases metabolism, Fibrin genetics, Fibrinogen genetics, Gene Expression, Glioma veterinary, Thromboplastin genetics, Thrombosis veterinary
- Abstract
In human gliomas, tissue factor (TF) is overexpressed, associated with the grade of malignancy and influences tumour biology. Intra-tumoural fibrin/fibrinogen deposition and activation of the fibrinolytic system also play a role in tumour cell proliferation and angiogenesis. The first aim of the present study was to investigate TF expression and the presence of fibrin/fibrinogen and D-dimers in canine glioma biopsies, graded according to the World Health Organization (WHO) classification of tumours of the central nervous system. The second aim was to investigate the occurrence of intravascular thrombosis (IVT) in canine gliomas, as a potential histological marker of glioma type or grade of malignancy. An immunohistochemical study using antibodies against TF, fibrin/fibrinogen and D-dimers was performed with 24 glioma samples, including 15 oligodendrogliomas, 6 astrocytomas and 3 mixed gliomas. Immunohistochemical data were statistically analysed to determine whether there was any relationship between glioma type and grade of malignancy. All gliomas were moderate to strongly positive for TF and the staining score was significantly higher (P = 0.04) in high-grade (III or IV) than in low-grade (II) gliomas. Intra-tumoural fibrin/fibrinogen deposition was detected in all tumour biopsies assessed, and D-dimers were detected in 17/24 gliomas. IVT was a frequent finding, but was not linked to a specific glioma type or malignancy grade. TF expression, fibrin/fibrinogen deposition, extravascular fibrinolytic system activation and IVT occur in canine gliomas. Canine glioma might be a suitable model for studying coagulation and fibrinolysis as potential therapeutic targets for human gliomas., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
4. Schmallenberg virus circulation in high mountain ecosystem, Spain.
- Author
-
Fernández-Aguilar X, Pujols J, Velarde R, Rosell R, López-Olvera JR, Marco I, Pumarola M, Segalés J, Lavín S, and Cabezón O
- Subjects
- Altitude, Animals, Bunyaviridae Infections epidemiology, Bunyaviridae Infections immunology, Bunyaviridae Infections transmission, Cattle, Cattle Diseases immunology, Cattle Diseases transmission, Disease Reservoirs, Ecosystem, Fetus virology, Goat Diseases immunology, Goat Diseases transmission, Goats, Orthobunyavirus immunology, Seroepidemiologic Studies, Sheep, Sheep Diseases immunology, Sheep Diseases transmission, Spain epidemiology, Antibodies, Viral blood, Bunyaviridae Infections veterinary, Cattle Diseases epidemiology, Deer virology, Goat Diseases epidemiology, Orthobunyavirus isolation & purification, Sheep Diseases epidemiology
- Published
- 2014
- Full Text
- View/download PDF
5. Severe outbreak of disease in the southern chamois (Rupicapra pyrenaica) associated with border disease virus infection.
- Author
-
Marco I, Lopez-Olvera JR, Rosell R, Vidal E, Hurtado A, Juste R, Pumarola M, and Lavin S
- Subjects
- Animals, Border Disease epidemiology, Border Disease mortality, Border Disease pathology, Brain pathology, Female, Geography, Goat Diseases mortality, Goats, Male, Seasons, Spain epidemiology, Border Disease virology, Border disease virus isolation & purification, Disease Outbreaks veterinary, Goat Diseases epidemiology, Goat Diseases virology, Rupicapra virology
- Abstract
An outbreak of a previously unreported disease affecting southern chamois (Rupicapra pyrenaica) in the central Pyrenees (NE Spain) was recorded in 2001 and 2002. There was a marked temporal distribution, most animals being found between February and June. After the outbreak, the population was found to have decreased by about 42%, most probably due to the disease. We examined 20 affected chamois. Clinical manifestations included depression, weakness and movement difficulties in all cases. Three chamois presented abnormal behaviour, with absence of flight reaction, and 16 showed different degrees of alopecia with skin hyperpigmentation. At necropsy cachexia was observed in all animals, four chamois had abscesses in different parts of the body, four had pneumonia, one had an extensive subcutaneous infection on the head and neck and one had severe orchitis. Microscopic lesions were found in the brain, mainly edema, gliosis, espongiosis, cariorrexis and neuronal multifocal necrosis. A perivascular mononuclear inflammatory infiltrate was present in three of them. Skin lesions included marked follicular atrophy, mild to moderate epidermal hyperplasia with orthokeratotic hyperkeratosis and follicular hyperkeratosis, and hypermelanosis. In 13 chamois there were haemosiderin deposits in the spleen, and in three individuals kidney "cloissone" was observed. Intraeritrocitic parasites were detected either by direct observation or PCR in 8 of 17 chamois. A pestivirus was isolated and detected by RT-PCR from 12 of 13 affected chamois and antigenic characterized as border disease virus by monoclonal antibodies. This is the first time a border disease virus has been associated with an outbreak of a high-mortality disease in a wild species.
- Published
- 2007
- Full Text
- View/download PDF
6. Immunohistochemical approach to the pathogenesis of bovine spongiform encephalopathy in its early stages.
- Author
-
Vidal E, Márquez M, Tortosa R, Costa C, Serafín A, and Pumarola M
- Subjects
- Animals, Brain pathology, Caspase 3, Caspases metabolism, Cattle, Encephalopathy, Bovine Spongiform pathology, Encephalopathy, Bovine Spongiform prevention & control, Female, Inflammation pathology, Metalloproteins metabolism, Spain, Synaptophysin metabolism, Synaptosomal-Associated Protein 25 metabolism, Time Factors, Brain metabolism, Encephalopathy, Bovine Spongiform metabolism, Immunohistochemistry, PrPSc Proteins metabolism
- Abstract
An immunohistochemical and histochemical study was carried out on the brains of nine cases of BSE-diagnosed cattle as part of the surveillance plan in Catalonia, Spain. The animals had no clinical symptoms reported and were thus at early stages of the disease. The first part of the study consisted of a characterization of PrP(BSE) deposits throughout the encephalon. The behaviour of the different immuno-labelling patterns was analysed and tropism of some patterns towards certain brain areas was described. This tropism is principally directed to the brain stem region; however, an association of the stellate pattern was found with areas where PrP(BSE) is deposited less abundantly, such as the cerebral cortex. Secondly, distinct pathogenesis mechanisms that take place in the early stages of BSE, which would include these cases were investigated. This study describes the glial response to the presence of PrP(BSE) (using antibodies against astrocytic glial fibrillary acidic protein and lectin from Griffonia simplicifolia to identify microglia), the presence of mild oxidative stress phenomena (antibodies against metallothioneins I and II and against nitrated aminoacidic residues: nitrotyrosine), the apparent absence of apoptotic cellular death (cleaved caspase 3) and the preservation of synaptic proteins synaptophysin and small synaptosome-associated 25 kDa protein immuno-labelling. Finally, no alteration of the extra-cellular matrix was detected with the use of Wisteria floribunda agglutinin, a marker for perineuronal nets.
- Published
- 2006
- Full Text
- View/download PDF
7. Distribution of PrP(res) in the brains of BSE-affected cows detected by active surveillance in Catalonia, Spain.
- Author
-
Sisó S, Ordóñez M, Cordón I, Vidal E, and Pumarola M
- Subjects
- Animals, Brain pathology, Brain Stem metabolism, Cattle, Encephalopathy, Bovine Spongiform pathology, Female, Spain, Brain metabolism, Encephalopathy, Bovine Spongiform metabolism, PrPSc Proteins metabolism
- Published
- 2004
- Full Text
- View/download PDF
8. Neospora caninum infection in a Napolitan mastiff dog from Spain.
- Author
-
Pumarola M, Añor S, Ramis AJ, Borràs D, Gorraiz J, and Dubey JP
- Subjects
- Animals, Antibodies, Protozoan blood, Brain parasitology, Brain pathology, Coccidiosis parasitology, Coccidiosis pathology, Dog Diseases parasitology, Dogs, Encephalomyelitis parasitology, Encephalomyelitis pathology, Fluorescent Antibody Technique, Male, Neospora immunology, Spain, Spinal Cord parasitology, Spinal Cord pathology, Coccidiosis veterinary, Dog Diseases pathology, Encephalomyelitis veterinary, Neospora isolation & purification
- Abstract
Fetal neosporosis-associated myeloencephalitis was diagnosed in a 4-month-old Napolitan mastiff dog from Spain. Neospora caninum tachyzoites and tissue cysts were observed in lesions in the central nervous system and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum monoclonal and polyclonal antibodies.
- Published
- 1996
- Full Text
- View/download PDF
9. Herpesvirus hepatitis in two eagles in Spain.
- Author
-
Ramis A, Majó N, Pumarola M, Fondevila D, and Ferrer L
- Subjects
- Animals, Birds, Herpesviridae isolation & purification, Herpesviridae Infections pathology, Liver pathology, Microscopy, Electron, Spain, Bird Diseases, Hepatitis, Viral, Animal pathology, Herpesviridae Infections veterinary, Liver ultrastructure
- Abstract
Two cases of naturally occurring fatal disease in eagles (a booted eagle and a buzzard) are reported. Both eagles showed anorexia, weight loss, weakness, and inability to fly. Microscopically, stained liver sections showed wide non-zonal coagulation necrosis and eosinophilic intranuclear inclusion bodies in hepatocytes adjacent to the edges of necrotic regions. Ultrastructural studies of hepatocytes revealed multiple enveloped viral particles in the cytoplasm, focal paracrystalline arrays of virions within the nuclei, and some budding particles bound by a membrane, located in an evagination of nuclear membrane. The size and morphology of all of these particles were consistent with herpesvirus. To our knowledge, this is the first report of naturally occurring field cases of herpesvirus hepatitis in eagles (Accipitridae).
- Published
- 1994
10. Pathologic and immunocytochemical studies of morbillivirus infection in striped dolphins (Stenella coeruleoalba).
- Author
-
Domingo M, Visa J, Pumarola M, Marco AJ, Ferrer L, Rabanal R, and Kennedy S
- Subjects
- Animals, Antigens, Viral analysis, Brain microbiology, Brain pathology, Female, Gingiva pathology, Immunohistochemistry, Liver pathology, Lung microbiology, Lung pathology, Lymph Nodes microbiology, Lymph Nodes pathology, Male, Mammary Glands, Animal microbiology, Mediterranean Sea, Palate pathology, Paramyxoviridae immunology, Respirovirus Infections epidemiology, Respirovirus Infections microbiology, Respirovirus Infections pathology, Spain epidemiology, Disease Outbreaks veterinary, Dolphins, Paramyxoviridae isolation & purification, Respirovirus Infections veterinary
- Abstract
Hundreds of striped dolphins (Stenella coeruleoalba) died along the Spanish Mediterranean coast during the second half of 1990. We necropsied 58 dolphins. Partial collapse of the lungs with patchy atelectasis, subcutaneous edema, icterus, and stomatitis were the most prominent gross morphologic changes. Histologically, a bronchiolo-interstitial pneumonia was the most frequent lesion (72% of the animals). It was characterized by hyperplasia of alveolar epithelial type II cells and formation of multinucleate syncytia in alveolar and bronchiolar lumina. Other prominent lesions were encephalitis (69%), lymphoid depletion, and formation of multinucleate syncytia in the cortex of lymph nodes. The distribution of morbillivirus antigen was investigated in 23 well-preserved dolphins using a monoclonal antibody against the hemagglutinin glycoprotein of phocine distemper virus. Positive immunostaining was found in brain (77%), in lung (70%), and in mesenteric (61%), mediastinal (47%), and prescapular (45%) lymph nodes. Phocine distemper virus antigen was demonstrated less frequently in trachea, stomach, biliary epithelium, intestine, kidney, and mammary gland. Necrotizing-hemorrhagic pneumonia and encephalitis due to Aspergillus fumigatus were seen in three dolphins, whereas two animals had lesions of toxoplasmosis. Changes in our dolphins were similar to those caused by distemper in seals and porpoises. The origin of the dolphin virus and the relationships among dolphin, seal, and porpoise morbilli viruses are unknown.
- Published
- 1992
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.