1. Chloroplast ultrastructure and thylakoid polypeptide composition are affected by different salt concentrations in the halophytic plant Arthrocnemum macrostachyum.
- Author
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Trotta A, Redondo-Gómez S, Pagliano C, Clemente ME, Rascio N, La Rocca N, Antonacci A, Andreucci F, and Barbato R
- Subjects
- Amaranthaceae growth & development, Amaranthaceae metabolism, Chloroplasts chemistry, Chloroplasts drug effects, Chloroplasts metabolism, Cytochromes metabolism, Photosystem I Protein Complex metabolism, Photosystem II Protein Complex metabolism, Plant Growth Regulators metabolism, Salt-Tolerant Plants growth & development, Salt-Tolerant Plants metabolism, Spain, Thylakoids metabolism, Thylakoids ultrastructure, Amaranthaceae ultrastructure, Chloroplasts ultrastructure, Membrane Proteins metabolism, Sodium Chloride pharmacology
- Abstract
The effect of different external salt concentrations, from 0 mM to 1030 mM NaCl, on photosynthetic complexes and chloroplast ultrastructure in the halophyte Arthrocnemum macrostachyum was studied. Photosystem II, but not Photosystem I or cytochrome b6/f, was affected by salt treatment. We found that the PsbQ protein was never expressed, whereas the amounts of PsbP and PsbO were influenced by salt in a complex way. Analyses of Photosystem II intrinsic proteins showed an uneven degradation of subunits with a loss of about 50% of centres in the 0 mM NaCl treated sample. Also the shape of chloroplasts, as well as the organization of thylakoid membranes were affected by NaCl concentration, with many grana containing few thylakoids at 1030 mM NaCl and thicker grana and numerous swollen thylakoids at 0 mM NaCl. The PsbQ protein was found to be depleted also in thylakoids from other halophytes., (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Published
- 2012
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