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This study deals with detecting the difficulties that prospective teachers encounter in recognising and analysing children's ideas in the domains of social studies and science. It qualitatively analyses the reports written by 94 third-year Early Childhood Education degree students, while they were taking courses on "Teaching the natural environment to age groups of 0 to 6 Years" and "Knowledge of the Social Environment." The results show that the titles prospective teachers use for their proposals titles do not arouse the children's interest, even when they are not being taken literally from the limited Spanish curriculum. Nevertheless, they demonstrate a great capacity for adapting to the children in terms of the language used in the instruments designed to detect the children's ideas. The greatest obstacle they find is in the analysis of the children's ideas, especially in the process of categorising the responses. Further work with prospective teachers is therefore necessary to provide them with experience of direct contact with children, and to accompany them in the detection and analysis of the children's ideas.

Purpose: The purpose of this paper is to analyse the presence of the sustainable development goals (SDGs) proposed by the UN (2015) in university degrees within the fields of education, humanities and environmental sciences (ES) at Andalusian public institutions (Spain). Design/methodology/approach: This paper shows an empirical analysis from a mixed methodological model on a total of 99 syllabi and training programs from nine different universities. The collection of information has been carried out through a rubric specifically designed within the framework of this body of research. Findings: The results show that the syllabus of the subjects in the faculties of education includes the SDGs related to the social aspect of sustainability, with special focus on SDG4, SDG5, SDG10, SDG16 and SDG17, whereas others like SDG6 and SDG7 are less represented. SDGs are present in the majority of syllabus of the subjects analysed. It is certainly a positive finding which shows predisposition and a high interest on by the teachers involved. However, this is not enough as there is still a long way to go until achieving a thorough and complete incorporation of the principles of sustainability. Originality/value: This research sheds light on the changes and transformations that the discourse linked to sustainability is generating in the university syllabi. Taking the SDG as a framework this paper highlights the most original aspects: a replicable methodology that allows diagnosing the level of curricular greening of the university syllabi is provided to other contexts the innovative value of connecting teaching with local and global environmental problems in their physical-chemical social and economic dimensions is shown and it has been possible to compare the difficulties of some universities in addressing compliance with the SDGs and curricular sustainability from a systemic and integrative perspective that will lead to methodological transformation and pedagogical renewal.

This study examines the association between nurses' perception of caring and attitudes toward caring for dying patients in end-of-life care units. We used a descriptive cross-sectional design with convenience sampling. Data were collected from May to June 2021 through self-reported questionnaires from 303 Spanish nurses (mean age = 48 years, 78.9% female). Participants completed the Caring Dimension Inventory (CDI-25) and the Frommelt Attitudes Toward Care of the Dying scale. Demographic and training information were collected. Data were analyzed using Stata 16.0. Attitudes toward Care of the Dying Patient were significantly higher in nurses with palliative care training (β = 2.829, p = 0.018) and those who scored higher on the psychosocial (β = 0.789, p = 0.0001) and technical dimensions (β = 0.487, p = 0.011) of the CDI-25. Multivariate analysis identified the psychosocial dimension of the CDI-25 scale (β = 0.783, p = 0.0001) and palliative care training (β = 2.686, p = 0.017) as the only significant variables associated with Attitudes toward Care of the Dying Patient. Overall, nurses exhibited a positive attitude toward caring for dying patients. Our results could potentially help nursing directors identify those with the necessary qualities and training for optimal end-of-life care and to further plan training courses to provide optimal care in end-of-life situations. [ABSTRACT FROM AUTHOR]

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Background: Despite the fact that prison inmates are a population at higher risk than other groups of suffering from intestinal parasite infections in relation to their living conditions, information about these diseases in prison environments is still scarce. Herein, we analyze the status of intestinal parasite infections in a Spanish prison. Methods: A cross-sectional study involving 528 inmates was conducted from April to June 2022 among inmates at Centro Penitenciario Picassent (Valencia, Spain). Stool specimens were examined using the direct wet mount technique, the formol-ether concentration technique, and the Ziehl-Neelsen staining method. We used STATA 16.1 for data analysis. We consider a p-value less than 0.05 significant at a 95% confidence level. Results: Of the 528 inmates (471 men and 57 women; a mean age of 41.94 years) enrolled in the study, 83 (15.7%) were infected. Only six species of protozoa were detected. The gut potential microeukaryotic pathobiont Blastocystis sp. was the predominant parasite, accounting for 37 (44.6%) of the infections. Gut parasite amebas (6.6%) and pathobionts (5.3%) were more prevalent than flagellates (2.3%). The prevalence of infection with pathogenic species (8.9%) was similar to that of non-pathogenic species (8.7%). Infection among men (15.2%) was higher than in women (0.6%) (p < 0.0001). In multivariate analysis, the country of birth (AOR = 0.31, 95% CI = 0.18–0.52) and the time spent in prison (AOR = 1.83, 95% CI = 1.06–3.14) were statistically significant associated to intestinal parasite infections (p < 0.0001 and p = 0.028, respectively). Conclusion: This study found low levels of intestinal parasite infections in the CPP which could be indicative of the implementation of sanitary measures in prison environments in Spain. The less time spent in prison favor the risk of having infection while the Spanish nationality of inmates could reduce the risk of infection. The main recommendation would be to introduce routine parasitological tests upon foreigners entering prison. [ABSTRACT FROM AUTHOR]

On March 11, 2004, Madrid suffered one of the worst terrorist attacks in the history of Spain, leaving more than 190 dead and 2,000 injured. For years, the psychological consequences of the attacks have been studied; however, its long-term effects on symptomatology and especially on well-being remains unknown. This study aims to explore, through a qualitative approach, pathways and obstacles to the well-being of those affected directly or indirectly by the attacks of March 11 in Madrid. Two focus groups were held, one for indirect victims and one for direct victims. Subsequently, a thematic analysis of the materials obtained was carried out. More than 10 years after the attacks, most of the participants reported great difficulty in achieving well-being. Acceptance and victims' associations seemed to act as key facilitators, while symptoms, political institutions and the media were the main obstacles. Direct and indirect victims presented similar data although aspects such as guilt and family relationships played a different role in their well-being. [ABSTRACT FROM AUTHOR]

In Spain, health competencies are decentralized, and each autonomous community implements its own plans. Our aim is to determine if the existence of regional palliative care plans implies an improvement in health care coverage. We reviewed regional palliative care plans published in Spain and analyzed the following variables: number and profile of palliative care resources, guidelines and objectives of implementation of resources of these plans, and compliance with European standards. The publication of regional plans is associated with an increase in specific resources in the following years. From 2004 to 2009, the implementation of plans in 6 autonomous communities was associated with an increase in palliative care resources compared to those without a strategy (odds ratio: 1.58, P =.02) or with a settled plan (odds ratio: 1.40, P =.07). The same phenomenon was observed between 2009 and 2015 in 4 autonomous communities and 2 autonomous cities compared with those without a strategy (odds ratio: 2.49, P =.001) and those that implemented a plan before 2009 (odds ratio: 2.62, P <.001). Updating and evaluating these regional plans are also associated with the growth of palliative care resources. [ABSTRACT FROM AUTHOR]

Recent new outbreaks of Bursaphelenchus xylophilus in Spain and adverse pine wilt disease expansion forecasts must be met with rapid advances in genetic breeding against this pathogen. The optimal approach for breeding trees that are more resistant to pine wilt disease is to use the most virulent isolates in inoculation assays. A series of controlled inoculation experiments on Botrytis cinerea cultures, along with Pinus pinaster and P. radiata branch sections and seedlings, was conducted to investigate differences among seven virulent B. xylophilus isolates of diverse geographic origin (USA745 from USA, KA4 and S10 from Japan, Pt52T and Pt72CH from Portugal, SpPO1 and SpSA1 from Spain). Results from the experiments showed significant differences among B. xylophilus isolates. The virulence classification of the isolates studied differed for P. pinaster and P. radiata, indicating that PWN isolates vary according to host specificity. Isolates Pt52T and SpP01 proved the most virulent to P. pinaster, so either of them could be used as 'test isolates' in future assays to identify resistant or tolerant genotypes. For P. radiata, any isolate except SpSA1 could be used, as all the others were more virulent and all presented the same significant level of virulence. The results indicate that use of P. pinaster branch sections, rather than P. radiata branch sections or multiplication assays on B. cinerea, could be an accurate, time‐saving method for evaluating virulence levels of new B. xylophilus isolates. [ABSTRACT FROM AUTHOR]

Background and objectives: Panic disorder (PD) is an anxiety disorder characterized by recurrent and unexpected panic attacks along with sudden onset of apprehension, fear or terror. The endocannabinoid system (ECS) has a role in stress recovery, regulating anxiety. The aim of this study was to analyze potential genetic alterations in key ECS targets in patients suffering from panic disorders.Design and methods: We analyzed single nucleotide polymorphisms (SNPs) of the cannabinoid receptors (CNR1; CNR2) and the endocannabinoid hydrolytic enzyme fatty acid amide hydrolase (FAAH) genes in 164 Spanish PD patients and 320 matched controls.Results: No significant differences were observed in the SNPs of the CNR2 and FAAH genes tested. However, when analyzing genotype-by-sex interaction at A592G (rs2501431) and C315T (rs2501432) in the CNR2 gene, the presence of the G-allele in males was associated with a protective haplotype. Genotyping analysis revealed that variants in CNR1 confer vulnerability to PD, with a significantly increased risk associated with the G-allele (rs12720071) and C-allele (rs806368). This finding was consistent when analyzing genotype-by-sex interaction, where females presented a greater PD risk.Conclusions: Polymorphisms at the CNR1 gene may be a risk factor for PD contributing to sex-specific dysfunction in females. [ABSTRACT FROM AUTHOR]

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Beef preparations (meatballs, minced meat, hamburgers, white sausages, and red sausages) from northwest Spain were tested. Microbial counts ranged from 0.70 ± 0.00 log10 cfu/g (enterococci) to 9.57 ± 0.37 log10 cfu/g (psychrotrophs). In 73.3% of cases, total aerobic counts were higher than the microbiological limits set for the end of the manufacturing process in the European Union (EU Regulation 2073/2005). Forty Escherichia coli isolates were tested against thirteen clinically important antibiotics (disk diffusion method; CLSI). Three of the strains (7.5%) were susceptible to all the antibiotics, four (10.0%) showed resistance or reduced susceptibility to one antibiotic, and 33 (82.5%) were multiresistant (with resistance or reduced susceptibility to between two and eight antibiotics), with an average of 1.85 resistances per strain. The highest rates of resistance were observed for two antimicrobials widely used on cattle farms (cefalotin and tetracycline). The findings in this research emphasize the need to correctly handle beef preparations with the aim of reducing risks to consumers. [ABSTRACT FROM AUTHOR]

Background and aims: Limited data are available on the effectiveness and tolerability of direct-acting antivirals (DAAs) therapies in the real world for HCV-infected patients with comorbidities. This study aimed to describe the effectiveness of OBV/PTV/r ± DSV (3D/2D regimen) with or without ribavirin (RBV) in HCV or HCV/HIV co-infected patients with GT1/GT4 and CKD (IIIb-V stages), including those under hemodialysis and peritoneal dialysis in routine clinical practice in Spain in 2015. Material and methods: Non-interventional, retrospective, multicenter data collection study in 31 Spanish sites. Socio-demographic, clinical variables, study treatment characteristics, effectiveness and tolerability data were collected from medical records. Results: Data from 135 patients with a mean age (SD) of 58.3 (11.4) years were analyzed: 92.6% GT1 (81.6% GT1b and 17.6% GT1a) and 7.4% GT4, 14 (10.4%) HIV/HCV co-infected, 19.0% with fibrosis F3 and 28.1% F4 by FibroScan®, 52.6% were previously treated with pegIFN and RBV. 11.1%, 14.8% and 74.1% of patients had CKD stage IIIb, IV and V respectively. 68.9% of patients were on hemodialysis; 8.9% on peritoneal dialysis and 38.5% had history of renal transplant. A total of 125 (96.2%) of 135 patients were treated with 3D, 10 (7.4%) with 2D and 30.4% received RBV. The overall intention-to-treat (ITT) sustained virologic response at week 12 (SVR12) was 92.6% (125/135) and the overall modified-ITT (mITT) SVR12 was 99.2% (125/126). The SVR12 rates (ITT) per sub-groups were: HCV mono-infected (91.7%), HCV/HIV co-infected (100%), GT1 (92.0%), GT4 (100%), CKD stage IIIb (86.7%), stage IV (95%) and stage V (93%). Among the 10 non-SVR there was only 1 virologic failure (0.7%); 4 patients had missing data due lost to follow up (3.0%) and 5 patients discontinued 3D/2D regimen (3.7%): 4 due to severe adverse events (including 3 deaths) and 1 patient´s decision. Conclusions: These results have shown that 3D/2D regimens are effective and tolerable in patients with advanced CKD including those in dialysis with GT 1 or 4 chronic HCV mono-infection and HIV/HCV coinfection in a real-life cohort. The overall SVR12 rates were 92.6% (ITT) and 99.2% (mITT) without clinically relevant changes in eGFR until 12 weeks post-treatment. These results are consistent with those reported in clinical trials. [ABSTRACT FROM AUTHOR]

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The article discusses experimentation conducted on methods of improving reading comprehension in high school students in Cantabria, Spain, during 2014 and 2015. Topics discussed include the requirement that a proposed teaching innovation be motivational for the students; the use of an unrelated or absurd sentence within the assigned text to improve content retention; and the testing of reading comprehension by requiring students to summarize what they had read prior to the absurd sentence.

Background: Confirmatory factor analytic studies have shown that posttraumatic stress disorder (PTSD) symptoms included in the fifth edition of the Diagnostic and Statistical Manual Disorders (DSM-5) may be better explained by two 6-factor models (the Externalizing Behaviours model and the Anhedonia model) and a 7-factor Hybrid model. The latter model comprises the symptom clusters of intrusion, avoidance, negative affect, anhedonia, externalizing behaviours, and anxious and dysphoric arousal. This model has received empirical support mainly in American samples. Of note, there have been a limited number of studies conducted on samples from other countries. Objective: This study aimed to examine the underlying dimensionality of DSM-5 PTSD symptoms in a Spanish clinical sample exposed to a range of traumatic events. Method: Participants included 165 adults (78.8% females) seeking treatment in trauma services in the Madrid area (Spain). PTSD was assessed using the Global Assessment of Posttraumatic Stress Scale 5, a Spanish selfreport instrument assessing posttraumatic symptoms according to the DSM-5 criteria. Confirmatory factor analyses were conducted in Mplus. Results: Both the 7-factor Hybrid model and the 6-factor Anhedonia model demonstrated good and equivalent fit to the data. Conclusions: The findings of this study replicate and extend previous research by providing support for both the 7-factor Hybrid model and the 6-factor Anhedonia model in a clinical sample of Spanish trauma survivors. Given equivalent fit for these two models and the fewer number of latent factors in the Anhedonia model, it was selected as optimal in a traumatized Spanish sample. Implications and future research directions are discussed. [ABSTRACT FROM AUTHOR]

¿Potencian las redes sociales online las relaciones interculturales entre los jóvenes migrantes? ¿Influyen este tipo de interacciones en la construcción de la identidad digital? Para dar respuesta a ambas cuestiones referimos los resultados de una parte del proyecto “Las relaciones sociales de los jóvenes migrantes en Internet desde la perspectiva de la interculturalidad" (CSO2011-24376). Partimos del concepto de racionalidad comunicativa de Jürgen Habermas como la lógica más conveniente para el florecimiento de interacciones comunicativas interculturales. Siguiendo a este autor se ha diseñado un modelo para analizar las interacciones en las redes sociales online declaradas por jóvenes migrantes y no migrantes en 13 grupos de discusión de Madrid, Barcelona y Bilbao. El modelo de análisis parte de las cuatro formas de acción social desarrolladas por Habermas (acción estratégica, acción regulada por normas, acción dramatúrgica y acción comunicativa) y extrae las dimensiones e indicadores con los que poder identifica las interacciones en las redes sociales online. Este estudio pone de manifiesto que las prácticas comunicativas online de los jóvenes son marcadamente dramatúrgicas, en tanto que se busca refrendar el valor de las representaciones propias y de los otros en términos de identidad digital. Por tanto, no responden a una racionalidad comunicativa de interacción que favorezca la comunicación intercultural. [ABSTRACT FROM AUTHOR]

This article investigates minority expropriation in closely-held firms. Using a sample of Spanish firms for the period from 1996 to 2006, we find that firms that are more vulnerable to minority expropriation have blockholders controlling groups with aggregate equity stakes that are far removed from 50%, which is the point that maximizes the chances of expropriation. Moreover, performance improves when the controlling group's stake moves away from the region where expropriation is more likely – the alignment effect – and, if within this region, when the number of group members increases – the bargaining effect. [ABSTRACT FROM AUTHOR]

The characteristics of current society and the interest about citizenship education in the context of lifelong learning are essential to a study that aims to describe and understand the development of social and civic competence in the educational field. A Delphi study can make a proposal in which the social and civic competence is composed of 89 items organized into five areas: knowledge, skills, attitudes, values and behaviours. In parallel, the study of a school in Sevilla discovers didactic strategies and organizational processes that benefit the development of the various components of this competence. [ABSTRACT FROM AUTHOR]

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Full understanding and control of pine wilt disease (PWD) is a work in progress and breeding for disease resistance constitutes an essential management strategy for reducing its impact, as evidenced by advanced breeding programs in countries such as Japan. Since Pinus radiata is one of the most commercially relevant species in northern Spain, we designed a study to assess genetic variation in susceptibility to this pathogen using 44 P. radiata half-sib families from the Galician breeding program. Three Bursaphelenchus xylophilus (pinewood nematode, PWN) inoculation experiments were performed to evaluate disease-related variables, estimate genetic parameters, and study sources of genotype by environment interaction (G × E). We also looked at differences in the constitutive chemical compounds of susceptible and non-susceptible individuals. The results showed great variation in PWN susceptibility, with survival rates for P. radiata families ranging from 0% to 90%. In addition, heritability estimates (hi2 = 0.43, hf2 = 0.72) and genetic gain (>26% selecting 50% of the families) were both moderately high for survival. Significant differences in several constitutive chemical compounds were found between susceptible and non-susceptible seedlings in the two susceptibility groups considered. These results confirm the potential of breeding to obtain P. radiata genotypes that are resistant to pine wilt disease and open possibilities for mitigating its future impact on P. radiata stands. [ABSTRACT FROM AUTHOR]

This study explores cultural ecosystem services (CES) provided by five arid watersheds of south-eastern Spain using the image social network Flickr. A total of 652 georeferenced photos were selected, uploaded by 174 authors. The most representative CES in each photo was identified. The relationships between the identified CES and the landscape elements and land uses represented by photos, as well as the CES associated with the protected areas of the study watersheds were examined by statistical and descriptive analyses. The results revealed that the CES most represented were aesthetic associated with water bodies, shrubs, native flora, trees and mountains; existence value with native fauna; inspiration associated with rocks and snow; and sense of place with natural and anthropised landscapes. Significant differences in aesthetic and existence value were found between photographs taken inside and outside protected areas. The use of photographs uploaded to social media in dryland is useful for exploring the landscape elements of greatest interest to citizens (e.g., landscapes, biodiversity) and some CES (e.g., aesthetic). However, other CES such as existential or inspirational value may be biased by subjective criteria of the researcher and due to the socio-cultural profile of the people who upload photographs to these networks. [Display omitted] • Social networks have become a useful tool for exploring cultural ecosystem services. • Aesthetic, existence and inspiration are the most represented CES in arid watersheds. • Several CES valued by the local population are not well represented in photographs. • Dry rivers seem invisible to photographers' eyes. • Social networks may be a proxy for exploring the CES of marginal ecosystems. [ABSTRACT FROM AUTHOR]

Abstract: In this study we determined the prevalence of genes coding for antimicrobial resistance, toxins, enzymes, immunoevasion and adhesins factors among 189 meticillin-resistant Staphylococcus aureus (MRSA) strains isolated from a third level hospital in Valladolid (Spain) between 2005 and 2008 in order to examine the relationship between these pathogenicity determinants, both individually and in combination, and the genetic background of main MRSA strains that are presents in Spanish hospitals. MRSA isolates were first characterised epidemiologically by a combination of molecular typing strategies like spa, SCCmec and multilocus sequence typing, and then, a cluster analysis based on pathogenicity factors genes was performed according to the hybridisation pattern of 65 virulence, 36 resistance, 15 adhesins, and 11 set/ssl genes on a Diagnostic DNA microarray (Alere StaphyType DNA microarray Jena, Germany). CC5-agr type II [ST125-SCCmecIV/VI (32.2%) or ST125-IV (19.1%), ST228-I (19.1%), ST146-IV (13.7%) and ST5- IV (0.5%)] isolates was widely distributed. CC8-agr type I [ST8-IV (11.5%), USA300 clone (0.5%), and ST239-III (1.1%)]; CC45-agr type II [ST45- IV (1.6%)], and the CC97-agr type I [ST97-IV] were also detected. We identified 42 different resistance genes profiles, 22 set/ssl genes profiles, and 91 different virulence profiles. However although the high genetic diversity of MRSA strains, mainly with respect to virulence factors genes, the results of the simultaneous assessment of resistance and virulence genes and the genetic background illustrated a correspondence relationship (p <0.001) between the different clones and same resistance and virulence genes or clusters of them. During the study period we observed changes in molecular epidemiology of MRSA isolates and as a consequence we report the changes of the resistance and virulence potential of MRSA strains produced over time in our institution. [Copyright &y& Elsevier]

Currently, Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) is being evaluated for its efficacy as a fast bacterial typing tool due to its great speed compared to other molecular methods. In this study, we evaluated MALDI-TOF as a tool for quick identification and typing of Francisella tularensis. This study encompassed 86 strains from two different geographical origins (Spain and the Czech Republic), which were previously characterised by Pulsed-Field Gel Electrophoresis (PFGE) and Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA). The direct colony method was used for microbial identification. High-quality spectra of the 86 strains were obtained and their main spectra profiles (MSPs) were created for epidemiological typing using MALDI-TOF. Based on the MSPs, principal components were generated and a dendrogram was constructed. An in-house MALDI-TOF library entry was created for each group of PFGE and MLVA strains based on their high-quality spectra. Two dendrograms were obtained using these entries and the unique peaks in each entry were searched. All strains were correctly identified to the species level. No clear divisions were found in the 86-strain dendrogram; however, Spanish and Czech strains appeared separately in dendrograms created using MLVA and PFGE entries. Entries from our in-house MALDI-TOF library revealed 2–4 biomarker peaks for the detection of the five PFGE groups and 1–12 biomarker peaks for the detection of the seven MLVA groups. Finally, two and one specific biomarkers were found in the Czech and Spanish strains, respectively. MALDI-TOF can be used to accurately identify F. tularensis strains in less than 15 min. Moreover, data on geographical origin and PFGE and MLVA groups could be obtained in less than one hour after colony growing. • Mass Spectrometry correctly identifies Francisella tularensis strains in less than 15 min, even if protein extraction is not performed. • A reliable clustering could not be achieved by the dendrogram obtained of all strains. • Dendrograms generated from the entries of our in-house library could be useful for the determination of the geographical origin of F. tularensis strains. [ABSTRACT FROM AUTHOR]