Search

This epidemiological survey aims to provide an update on the main flea species that parasitize domestic animals in the Western Andalusia assessing several ecological features that could be considered as possible risk factors for flea infestation. Over a 19‐month period (June 2021 to January 2023), we obtained a total of 802 flea samples from 182 dogs (Canis lupus familiaris, Carnivora: Canidae, Linnaeus, 1758) and 78 cats (Felis silvestris catus, Carnivora: Felidae, Schreber, 1775). For each parasitized host, an epidemiological survey was completed, including the following information: geographical origin, age, sex, rural or urban habitat, type of animal's lifestyle (domestic or non‐domestic), health status, cohabiting or not with other animals and the total number of collected fleas. The most common species was Ctenocephalides felis (Siphonaptera: Pulicidae) (Bouché, 1835) with a total of 713 specimens, which accounted for 89% of the total fleas. The second most abundant species was Pulex irritans (Siphonaptera: Pulicidae) (Linnaeus, 1758) with a total of 46 collected fleas (6% of the total). The remaining species identified were Archaeopsylla erinacei (Siphonaptera: Pulicidae) (Bouché, 1835) (25 specimens), Spilopsyllus cuniculi (Siphonaptera: Pulicidae) (Dale, 1878) (12 specimens) and Ctenocephalides canis (Siphonaptera: Pulicidae) (Curtis, 1826) (6 specimens), which accounted for 3%, 1% and 1%, respectively, of the total fleas collected. The months with the highest number of collected fleas were, in ascending order, May 2022, September 2021 and July 2021. Dogs had a greater diversity of flea species, and flea sex ratios were female biased in all identified species and among all studied hosts. Finally, we identified some potential host risk factors that promoted higher flea intensities, such as living in rural areas, or presenting other pathologies. [ABSTRACT FROM AUTHOR]

Human activities and infrastructures can disturb wildlife and alter their behaviour by triggering antipredator responses such as changes in time allocation to different behaviours. For instance, disturbance sources like traffic can cause significant variation in allocation of time to vigilance and foraging behaviours, which can be used as sensitive measures of the effects of human disturbance on animals. Such changes may ultimately result in physiological and ecological costs for the species. We used a unique opportunity to obtain direct behavioural observations of European wildcats, Felis silvestris , in the Cantabrian Mountains (northwest Spain) to investigate how different sources of anthropogenic disturbance influence time allocation of wildcats to specific behaviours. We also analysed how traffic affects hunting and vigilance time budgets as well as hunting success. We found that wildcats allocated more time to being alert and less time to hunting, feeding and moving in the presence of humans than in undisturbed situations. Wildcats also allocated more time to hunting when traffic levels were high, near roads and further from villages. Similarly, they dedicated more time to vigilance behaviours while feeding in the presence of moving vehicles. Finally, hunting success in the presence of vehicles seemed to decrease more for female wildcats than for males although this effect was not significant. In conclusion, humans and traffic influence the behaviour of wildcats inhabiting pastoral anthropogenic landscapes, although it is difficult to know to what extent such changes may translate into demographic effects. • Human presence increased time spent alert and reduced foraging behaviour. • Wildcats spent more time hunting in scenarios with high traffic and closer to roads. • Wildcats increased vigilance time while foraging in the presence of vehicles. • Hunting success was not significantly influenced by the presence of vehicles. • Humans and traffic affect wildcat behaviour in pastoral landscapes. [ABSTRACT FROM AUTHOR]

We describe a hemimandible of a medium-sized feline from the Middle Miocene (middle Aragonian, MN 5, local biozone Dc, around 15.5 Ma) site of Príncipe Pío-2, a recently discovered fossil locality placed in the urban limits of Madrid city (Spain), within an area previously known for its rich paleontological findings. The specimen is very well preserved, showing the complete hemimandible, as well as all the teeth except the incisors. Some characters, such as the missing talonid in m1, the high p3, the large mesial cuspid in p4, and the presence of a vestigial m2, prevent its adscription to other known feline taxa such as Styriofelis turnauensis or Miopanthera lorteti, previously recorded in the Middle Miocene faunas from Europe, but absent in the central basins of Spain. The hemimandible has robust mandibular corpus and ramus compared with these latter and to extant felines of similar size, and exhibits a relatively stronger ridged masseteric line, which points towards the existence of a proportionally larger and more powerful m. masseter that generated a strong bite when hunting. These characters suggest that the Príncipe Pío-2 feline could have preyed upon relatively larger prey than those of extant, similarly sized felines. We propose the new genus and species Magerifelis peignei for the studied specimen, a species that would also include the material from the French Middle Miocene localities of Bézian and Artenay. [ABSTRACT FROM AUTHOR]

The fossil record of lynxes provides clear evidence of a large range across the North Hemisphere during the Pliocene and Pleistocene. However, their origin, systematics and evolutionary relationships are still fraught with difficulties and controversy. Here we report a complete hemimandible of a medium-sized felid from the Early Pleistocene (MN17, middle Villafranchian, 2.05 Ma) site of La Puebla de Valverde (Teruel, Spain). Based on comparative and multivariate analyses of the lower dentition of 458 individuals of medium-sized Lynx, Caracal and Leptailurus, we confidently ascribe the remains to Lynx aff. issiodorensis. Although the dental proportions are somewhat different from those of the Eurasian L. issiodorensis (smaller canines and more elongated p4/m1), Lynx aff. issiodorensis shows affinities with the Issoire lynx from the contemporaneous site of Saint Vallier (France), sharing a similar morphology of the mandible, reduced canines, and long m1. We further test the hypothesis that examines the presence of the African/Asian Caracal in the European Plio/Pleistocene for C. depereti and C. issiodorensis, and discard the attribution of L. issiodorensis into Caracal. This mandible extends the record of the genus and contributes to update our understanding of the Lynx lineage and its variability within the European fossil record. [ABSTRACT FROM AUTHOR]

Traditional agro‐pastoral practices are more beneficial for biodiversity than intensified agricultural systems. Promotion of the growth of natural herbaceous vegetation in pastoral fields can enhance rodent populations and consequently influence ecological aspects of carnivores with rodent‐based diets, like prey consumption in the European wildcat (Felis silvestris). In this article, we investigated the effects of pastoral field extent, season and prey abundance on wildcat consumption of several prey species in the Cantabrian Mountains (NW Spain). Prey consumption in areas with presence of pastoral fields (even in low proportions) was dominated by profitable field‐dwelling rodent species such as Arvicola monticola. Consumption of Arvicola was not correlated with its abundance and was higher during summer and autumn. Apodemus dominated the wildcat diet in areas with higher forest proportion and far from pastoral fields, particularly during spring. Our results suggest that varying habitat use and seasonal changes in prey accessibility may determine wildcat prey consumption in pastoral landscapes. Our results can contribute to highlight the potential benefits of traditional and sustainable pastoral activities for the conservation of the European wildcat across its distribution range. [ABSTRACT FROM AUTHOR]

The aetiology and epidemiology of vector borne apicomplexan Babesia and Hepatozoon and kinetoplastid Leishmania infantum infections in wildlife have not been explored in wide areas of southern Spain. We investigated these infections in 151 wild carnivores, including foxes, badgers, beech martens, hedgehogs, wild cats, Egyptian mongooses, otters, genets and racoons. Overall, Hepatozoon, Babesia and L. infantum infections were detected in 68%, 48% and 23% of the wild animals surveyed, respectively. L. infantum‐infected wildlife were more likely to be also infected with the apicomplexan Hepatozoon and Babesia spp. compared to the non‐infected counterparts (p <.05). We report for the first time Hepatozoon martis in badgers and wild cats and H. canis in beech martens, and a Babesia sp. in wild cats from Spain. Our results also indicate the widespread distribution of H. canis in foxes (91%) and beech martens (13%), H. martis in beech martens (81%), wild cats (20%) and badgers (13%), H. felis in wild cats (60%), B. vulpes in foxes (64%), Babesia sp. type A closely related to B. vulpes, in badgers (58%) and Babesia sp. in wild cats (20%). Moreover, L. infantum infection was found in foxes (29%), beech martens (13%), badgers (8%) and 1/3 Egyptian mongooses. We also detected Cytauxzoon sp. in a wild cat and the first Sarcocystis sp. in a genet. This study provided evidence of increased risk of L. infantum infection in wildlife animals co‐infected with Babesia spp. or Hepatozoon spp. and indicated that these infections are widespread in wild carnivores from Spanish Mediterranean ecosystems. [ABSTRACT FROM AUTHOR]

Hepatitis E virus (HEV) is an emerging zoonotic pathogen that is currently recognized as one of the major causes of acute human hepatitis worldwide. In Europe, the increasing number of hepatitis E cases is mainly associated with the consumption of animal food products or contact with infected animals. Dogs and cats have been suggested as a zoonotic source of HEV infection. The aim of this study was to assess Orthohepevirus circulation, including HEV‐A, HEV‐B and HEV‐C species, in sympatric urban cats and dogs in southern Spain. Between 2017 and 2020, blood samples were collected from 144 stray cats and 152 dogs, both strays and pets. The presence of antibodies against HEV were tested using a double‐antigen sandwich ELISA and seropositive samples were further analysed by western blot. A RT‐PCR was performed to detect RNA of Orthohepevirus species (HEV‐A, HEV‐B and HEV‐C). A total of 19 (6.4%; 95%CI: 3.6‐9.2) of the 296 animals tested showed anti‐HEV antibodies by ELISA. Seropositivity was significantly higher in dogs (9.9%; 15/152; 95%CI: 5.1‐14.6) than in cats (2.8%; 4/144; 95%CI: 0.1‐5.5). Ten of the 18 ELISA‐positive animals that could be further analysed by western blot, reacted against HEV‐3 and/or HEV‐C1 antigens, which suggest circulation of both genotypes in urban cats and dogs in the study area. However, HEV‐A, HEV‐B and HEV‐C RNA were not detected in any of the tested sera. This is the first study to assess HEV circulation in both stray cats and dogs in Europe. Our results provide evidence of HEV exposure in sympatric urban cat and dog populations in southern Spain. Further studies are needed to determine the role of these species in the epidemiology of HEV. [ABSTRACT FROM AUTHOR]

Numerous studies have unsuccessfully tried to unravel the definitive host of the coccidian parasite Besnoitia besnoiti. Cattle infections by B. besnoiti cause a chronic and debilitating condition called bovine besnoitiosis that has emerged in Europe during the last two decades, mainly due to limitations in its control associated with the absence of vaccines and therapeutical tools. Although the exact transmission pathways of B. besnoiti is currently unknown, it is assumed that the parasite might have an indirect life cycle with a carnivore as definitive host. Current lack of studies in wildlife might underestimate the importance of free‐living species in the epidemiology of B. besnoiti. Thus, the aim of the present study is to assess the presence of Besnoitia spp. in free‐ranging mesocarnivores in Spain. DNA was searched by PCR on faeces collected from wild carnivores as a first approach to determine which species could be considered as potential definitive host candidates in further research. For this purpose, a total of 352 faecal samples from 12 free‐living wild carnivore species belonging to the Canidae, Felidae, Herpestidae, Mustelidae, Procyonidae and Viverridae families were collected in seven Spanish regions. PCR testing showed that Besnoitia spp. DNA was present in four faecal samples from red foxes collected in western Spain, an area with the greatest density of extensively reared cattle and associated with high incidence of bovine besnoitiosis in the country. To date, this is the first report of a B. besnoiti‐like sequence (99.57% homology) from carnivore faeces in a worldwide context. Red foxes might contribute to the epidemiology of B. besnoiti, although further studies, mostly based on bioassay, would be needed to elucidate the accuracy and extent of these interesting findings. [ABSTRACT FROM AUTHOR]

How sabertoothed felids have evolved their iconic morphology remains unclear because of the patchy fossil record of early machairodontines. Batallones localities in the Madrid region (Spain) have the potential to clarify this as two sites have yielded hundreds of fossils of the early machairodontines Promegantereon ogygia and Machairodus aphanistus. Previous analyses suggested that these two sites are not contemporaneous and a morphological drift between cavities was described for these two species; characterizing intraspecific variability is thus important to better understand the evolution of machairodontines. To tackle this issue, we modelled 62 felid mandibles in 3D using a laser scanner. We applied 3D geometric morphometrics (3D GM) and linear morphometrics on these models to test for differences in populations and to better characterize the morphology of early machairodontines. Both linear measurements and 3D data reveal an absence of morphological changes in mandible shape between the two sites. Batallones machairodontines are closer to felines than to other, more derived machairodontines in mandibular morphology, suggesting the existence of rapid shift in the mandibular shape between primitive and derived members of the clade. Our analysis did not reveal any allometric relationship between the overall shape of the mandible when studied with 3D GM and body size. Finally, we reveal a previously overlooked diversity in felid mandibular condyles, with machairodontines having much larger and medially inclined condyles. [ABSTRACT FROM AUTHOR]

Background: Pets may be carriers of infectious agents including parasites. As part of a larger-scale study covering the whole of Europe, this study examines deworming measures reported by Spanish pet owners and identifies risk factors. Methods: An online questionnaire was administered to cat and dog owners in Spain. The replies provided were used to obtain information about the pets' living conditions and to accordingly classify each pet into one of the four ESCCAP infection risk categories (A, B, C or D) for which different deworming frequencies are recommended. Questions were also asked about pet care and owners' attitude toward their pets. The Kruskal-Wallis test was used to correlate risk groups with deworming frequencies. Results: Completed questionnaires were returned by 500 cat owners and 501 dog owners. According to responses, 96.21% of dogs were assigned to risk category D (maximum risk), and only 1.2%, 2.2% and 0.4% to A, B and C, respectively. Almost all cats were assigned to the minimum risk category A (indoor cats, 62%) or maximum risk category D (outdoor cats, 32.8%); only 3.4% and 1.8% of cats were classified as risk B and C respectively. More dogs were allocated to the higher risk group compared to cats, which were more frequently kept indoors. Cats were reportedly dewormed less frequently than dogs (2.56 and 3.13 times per year respectively), consistent with their different infestation risk. Thus, pets in the lower risk group A were either adequately dewormed or treated more often than necessary. Only a small proportion of cats were not dewormed at all (n = 14). Alarmingly, almost all pets in risk groups B, C or D (representing 95% of dogs and 39% of cats) were dewormed less often than recommended. Conclusions: More effective health education is required for the management of zoonotic endoparasite diseases under the umbrella of One Health targeted at owners, veterinarians, general practitioners, and health authorities. To align deworming frequency with infection risk, pet owners should be provided with clear, compelling instructions. [ABSTRACT FROM AUTHOR]

Understanding predator-prey relationships is fundamental to develop effective conservation plans. Between 2015 and 2018, we combed 21 transects, each 7km long, searching for Iberian Lynx Lynx pardinus scat within the province of Madrid in central Spain. In order to minimise inherent subjectivity of visual identification as much as possible, we performed a double specific nested polymerase chain reaction (PCR) followed by a primer extension assay addressed to two Iberian Lynx diagnostic single nucleotide polymorphisms. Forty-six scat samples were positively identified as belonging to Iberian Lynx through genetic analysis. From these, we extracted remains of consumed prey, which we determined to the lowest possible taxonomic level, mainly through hair identification. Identified prey was divided into four types: lagomorphs, small mammals, birds, and ungulates. The species' diet composition was described based on the frequency of occurrence (FO) of each prey and niche breadth, and also compared with prior knowledge of the species using four prior studies as a comparative reference through the calculation of the niche overlap value. The FO of lagomorphs (39%) was the lowest, while the FO of small mammals (54%) was the highest recorded to date. The niche breath (0.36) was higher than recorded in prior studies, but still showing the specialist character of the Iberian Lynx. Niche overlap was low (C = 0.49), showing differences in trophic niche between the population in our study area and the one studied in southern Spain. This indicates that the Iberian Lynx is adept at switching its main prey, an ability that has previously been firmly rejected. It is, however, capable of adapting to alternative prey more often than recorded to date, which could be a behavioural response to the patchy distribution of European Rabbit Oryctolagus cuniculus in the study area. [ABSTRACT FROM AUTHOR]

Knowledge of population dynamics of threatened species in the wild is key to effective conservation actions. However, at present, there are many examples of endangered animals for which their current situation is unknown, and not just in remote areas and less developed countries. We have explored this topic by studying the paradigmatic case of the European wildcat (Felis silvestris silvestris), an endangered small carnivore whose status has been subjectively established on the basis of non-systematic approaches and opportunistic records. Little is known about its demographic situation, prompting the need for information to improve conservation measures. However, the secretive behaviour of felines along with its low density in natural conditions have prevented the gathering of sufficient data. We developed a field sampling strategy for one of the largest populations (Andalusia, South Spain, 87,268 km2), based on a logistically viable systematic non-intrusive survey by camera-trapping. This study offers the first large-scale estimation for any European wildcat population, based on analytical approaches applied on Species Distribution Models. A hierarchical approach based on a Maxent model for distribution estimation was used, along with Generalised Linear Models for density estimation from explicit spatial capture-recapture data. Our results show that the distribution range is smaller and more highly fragmented than previously assumed. The overall estimated density was very low (0.069 ±0.0019 wildcats/km2) and the protected areas network seems to be insufficient to cover a significant part of the population or a viable nucleus in demographic terms. Indeed, the most important areas remain unprotected. Our main recommendations are to improve the protected area network and/or vigilance programs in hunting estates, in addition to studying and improving connectivity between the main population patches. [ABSTRACT FROM AUTHOR]

Adult attitudes toward animals have received extensive research attention. By contrast, despite the importance of child–animal interactions for children's development and animal welfare, children's attitudes toward animals have not been fully explored. The aim of this study was, therefore, to examine Spanish children's attitudes toward animals. A 12-item scale, the Brief Attitudes Towards Animals scale for Children (BATAC), was designed and completed by 416 Spanish primary school children aged between 6 and 13 years. Analyses revealed that the attitude scale had very good internal consistency (Cronbach's a = 0.75; Revelle's omega = 0.75; Sijtsma's glb = 0.84) and three factors labelled "Compassion," "Friendship," and "Opinion on Ownership" explained 56.47% of the variance. The subscales were used in subsequent analyses alongside the total score. Demographic variables, such as age, school year group, ownership of a companion animal, and children's beliefs about animal mind, were shown to be associated with children's attitudes toward animals. Being older, in a higher school year, having a dog or a small mammal at home, and scoring animals higher on sentience capabilities were associated with higher pro-animal attitudes. Other pet types (i.e., cats, birds, reptiles or fish) and children's gender were not associated with attitudes to animals. This study is the first to explore attitudes toward animals among Spanish primary school children, and it highlights attitudinal differences regarding animal species and child demographic variables. [ABSTRACT FROM AUTHOR]

Copyright of Revista Brasileira de Reprodução Animal is the property of Revista Brasileira de Reproducao Animal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Enterocytozoon bieneusi is an obligate intracellular protist-like fungi parasite that infects numerous mammal hosts including humans, raising concerns of zoonotic transmission. There is little information available on the presence and diversity of E. bieneusi genotypes in companion animals. Here, we determined the occurrence and genetic diversity of E. bieneusi in domestic dogs and cats from Northern Spain. A total of 336 genomic DNA samples extracted from canine (n = 237) and feline (n = 99) faecal specimens were retrospectively investigated. The presence of E. bieneusi was assessed by PCR of the rRNA internal transcribed spacer (ITS) gene. The parasite was detected in 3.0% (3/99) and 0.8% (2/237) of the cats and dogs examined, respectively. All three feline positive samples were from stray cats living in an urban setting, whereas the two canine samples were from owned dogs living in rural areas. Sequence analysis revealed the presence of two genotypes in dogs, BEB6 and PtEb IX, and two genotypes in cats, D and Peru11. The identification of Peru11 in a cat and BEB6 in a dog constitutes the first report of those genotypes in such hosts as well as first report in Spain. This is also the first evidence of genotype D in cats and PtEb IX in dogs in Spain. Three out of the four genotypes, BEB6, D and Peru11, have been previously reported as human pathogens and are potentially zoonotic indicating that dogs and cats need to be considered potential sources of human infection and environmental contamination. [ABSTRACT FROM AUTHOR]

In this paper, we study a sample of cranial, mandibular and dental remains of two species of the machairodontine felid genus Machairodus from the Early Vallesian (MN 9, around 10 Ma) site of Los Valles de Fuentidueña (Segovia, Spain): the tiger-sized Machairodus aphanistus, and the smaller and more primitive M. alberdiae; a species which is only known from this site. The fossils of these two sympatric populations are compared with the most abundant samples of M. aphanistus from the Late Vallesian (MN 10), younger sites of Batallones-1 and Batallones-3 (Torrejón de Velasco, Madrid, Spain). The results support the specific separation of M. alberdiae from M. aphanistus based on several differences in teeth size and proportions. Besides this, we observed differences among the analysed samples of M. aphanistus, indicating a differentiation between the older and more primitive form from Los Valles de Fuentidueña, and the younger and more derived one from the two Batallones sites. These differences fit well with the morphological evolution of this lineage towards the more derived Turolian Amphimachairodus giganteus. Finally, a relatively structured, patched habitat, combining the presence of open landscapes with shrubby and wooded areas, is inferred to explain the sympatric distribution of these two large machairodontines in Los Valles de Fuentidueña. [ABSTRACT FROM AUTHOR]

The main objective of this brief communication is to inform about the exposure to certain pathogens of interest for mesocarnivores in wildcats (Felis silvestris silvestris) that inhabit a human-domestic-wild ecotone located in a Natural Park (Serranía de Cuenca, Central Spain). Blood and mucosal swabs (nasal, conjunctival and rectal) samples were collected from nine alive animals to detect canine distemper virus (CDV), parvovirus (CPV/FPV), feline leukaemia virus (FeLV), feline coronavirus (FCoV), feline immunodeficiency virus (FIV), Leptospira interrogans, Chlamydia felis, Ehrlichia canis, Toxoplasma gondii, and Neospora caninum. ELISA, immunochromatograpy, microscopy agglutination test and PCR assays were used. The results show the first worldwide detection of exposure of wildcats to L. interrogans (3 positive/9 analysed) and the first detection of exposure to CVD (7/9), of carriers of C. felis (2/9) and of fecal spreading of CPV-FPV (2/9) in wildcats in Spain. Exposure to T. gondii and CPV-FPV was detected in 5 of the 9 wildcats analysed, and to FelV in 4 of 9. No FIV, FCoV, Ehrlichia canis and Neospora caninum were detected. The results reveal the circulation of pathogens among the wildcat population studied, but more vigilance is needed for an accurate assessment of the impact of these pathogens on the health status of this population. [ABSTRACT FROM AUTHOR]

How species use space and time is central in ecology and evolution as well as for the conservation and management of vulnerable species. Unfortunately, this information is scarce for elusive species such as the threatened European wildcat (Felis silvestris silvestris), especially in Mediterranean areas. Using radiotracking and cameratrapping, we studied the habitat selection and the diel activity of a wildcat population in SE Spain, with particular emphasis on the relationships between wildcats and their main prey, the European rabbit (Oryctolagus cuniculus). First, we used Generalized Linear Mixed Models (GLMMs) to analyse the insuence of vegetation, rabbit abundance, topography and humanization on wildcat presence at several spatial scales and according to wildcat activity. Second, we used Spearman's rank correlations to assess the spatial relationship between the relative abundance indexes (RAIs) of wildcats and rabbits. Third, we used kernel density estimates to determine the daily activity patterns of wildcats and rabbits, and compared them by calculating the coeficient of overlap Δ. Patch complexity, rabbit abundance, slope and cover of dense scrubs were the most influential variables on wildcat presence. However, the effects of some environmental variables were scale-dependent. The relationship between rabbit abundance and wildcat presence was positive for the smaller scales and, unexpectedly, negative for the largest one. This result was supported by the quadratic relationship between wildcat RAI and rabbit RAI, indicating that wildcats generally avoided the highest rabbit abundance areas. We observed a typical nocturnal activity for wildcats, which surprisingly did not synchronize with the rabbit activity patterns. Rabbit antipredatory strategies, human pressure avoidance behaviour in wildcats and competition with other carnivores could be behind the observed spatiotemporal mismatch between wildcats and rabbits. Overall, our study highlights the importance of combining spatial and temporal approaches to obtain an integrative view of how resources are exploited by wildlife. [ABSTRACT FROM AUTHOR]

Background: The only natural hosts of Pseudorabies virus (PRV) are members of the family Suidae (Sus scrofa scrofa). In species other than suids infection is normally fatal. In these mammals, including carnivores, PRV typically causes serious neurologic disease. The endangered Iberian lynx (Lynx pardinus) is a wild feline endemic to southwestern Europe (Iberian Peninsula). The Iberian lynx was found to be the world's most endangered felid species in 2002. In wild felines, PRV infection has only been previously reported once in a Florida panther in 1994. No seropositive lynxes have ever been found, nor has PRV been detected in dead Iberian lynxes to date. Case presentation: We describe the first reported case of pseudorabies in an Iberian lynx (Lynx pardinus). Pseudorabies was diagnosed in a young wild Iberian lynx from Extremadura (SW Spain) by histopathological examination, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. Gross lesions included alopecia of the ventral neck, bloody gastro-intestinal contents and congestion of the brain. Histopathological analysis showed a moderate nonsuppurative meningoencephalitis with diffuse areas of demyelination, necrotizing gastritis and enteritis of the small intestine. Pseudorabies virus (PRV) antigen was found in neuronal and nonneuronal cells of the brain, tonsils, and gastric glandular epithelial cells by immunohistochemical analysis. The presence of the virus in the brain was confirmed by nested PCR. The sequence analysis of the 146 bp fragment (from the viral glycoprotein B gene) showed that the amplified sequence matched (with 100% identity) the PRV genome. Furthermore, specific DNA from glycoprotein D and E encoding-genes was detected by conventional and real-time PCR, respectively, confirming the latter that this infection was produced by a wild-type PRV strain. Conclusions: This study supports the suspicion that PRV could infect the Iberian lynx. The detection of PRV in a dead Iberian lynx suggests that the virus may have a negative impact on the survival of endangered lynxes in the wild. However, because this is the first verified instance of lynx mortality resulting from pseudorabies, its true impact on the population is unknown. [ABSTRACT FROM AUTHOR]

Pleistocene skinning and exploitation of carnivore furs have been previously inferred from archaeological evidence. Nevertheless, the evidence of skinning and fur processing tends to be weak and the interpretations are not strongly sustained by the archaeological record. In the present paper, we analyze unique evidence of patterned anthropic modification and skeletal representation of fossil remains of cave lion (Panthera spelaea) from the Lower Gallery of La Garma (Cantabria, Spain). This site is one of the few that provides Pleistocene examples of lion exploitation by humans. Our archaeozoological study suggests that lion-specialized pelt exploitation and use might have been related to ritual activities during the Middle Magdalenian period (ca. 14800 cal BC). Moreover, the specimens also represent the southernmost European and the latest evidence of cave lion exploitation in Iberia. Therefore, the study seeks to provide alternative explanations for lion extinction in Eurasia and argues for a role of hunting as a factor to take into account. [ABSTRACT FROM AUTHOR]

The Spanish fossil localities of Batallones-1 and Batallones-3 are two Vallesian (Late Miocene) natural traps known for their high concentration of carnivoran mammals. These include amphicyonids, ailurids, mustelids, ursids, hyaenids, machairodont felids, and two species of small-bodied felines, one the size of an European wildcat, Felis sylvestris, and the other the size of a caracal, Caracal caracal. Although these felines are much less abundant at the sites than their larger relatives, the sabre-toothed felids, the available samples from Batallones-1 and Batallones-3 are rich; they include postcranial elements, skulls and mandibles of the smaller species, while the larger feline species is solely known from postcranial elements recorded at Batallones-1. The small feline from Batallones-1 and Batallones-3, although showing similarities with the Turolian felines, is more related to the Middle Miocene genus Styriofelis Kretzoi, 1929, and thus we propose for it the new species name Styriofelis vallesiensis. On the other hand, the new generic name Pristifelis is proposed for the species Felis attica, which shows important differences with extant Felis. [ABSTRACT FROM PUBLISHER]

Abstract: Multiple species of metastrongylid lungworm (Nematoda: Metastrongyloidea) have been reported to infect members of the Felidae. This study describes two metastrongylid species infecting cats in Ibiza, Spain, including clinical features of infection and diagnosis via morphological and molecular characterisation of larval stages. Cats (n =7) presented with suspect lungworm infection, exhibiting coughing and other respiratory signs of infection. Faecal samples were collected from each cat and were subjected to the Baermann method for the detection of first stage larvae. In four cats, two different species of larvae were observed on the basis of morphology and were further molecularly characterised by PCR and sequencing of the 18S rRNA gene. Sequence data confirmed the presence of Aelurostrongylus abstrusus and an unknown species of Troglostrongylus. Molecular characterisation of Oslerus rostratus is also reported for the first time. Given the diversity of metastrongylid species capable of infecting cats, and morphological similarity of larval stages, an emphasis should be placed on the use of molecular characterisation for accurate diagnosis of infection. [ABSTRACT FROM AUTHOR]

The article discusses a study which compared five habitat types in relation to the occurrence of European wildcats in central Spain. Sampling on a series of transects was carried out between 2001 and 2002 in search of wildcat scats to calculate an abundance index. The study also estimated and summarised the structural variables of landscapes and rabbit abundance as orthogonal factors using a principal component analysis (PACA). It was shown in a priori contrasts that wildcats tended to be more abundant in areas with Mediterranean mountain vegetation, although agricultural steppes also provided suitable habitat.

Simple Summary: Sarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide-distributed skin infestation with a wide range of hosts, among them several species within the Felidae family. Sarcoptes scabiei was diagnosed in a dead adult female European wildcat (Felis silvestris silvestris) from Spain. This is the first description of Sarcoptes scabiei in a European wildcat. Since this is a species of conservation concern due to its critical demography in the southernmost population of the Iberian Peninsula, the impacts of infectious diseases, including sarcoptic mange, should be considered during disease surveillance programs of the species' populations. Sarcoptic mange caused by the mite Sarcoptes scabiei is a worldwide-distributed skin infestation with a wide range of hosts, among them several species within the Felidae family. Sarcoptes scabiei was diagnosed in a dead adult female European wildcat (Felis silvestris silvestris) from Spain, based on histological evaluation of skin biopsies and identification of the arthropod from skin scrapings and molecular methods. This is the first description of Sarcoptes scabiei in a European wildcat. Due to its critical demography in the southernmost population of the Iberian Peninsula, the impacts of infectious diseases, including sarcoptic mange, as a new potential threat should be considered during disease surveillance programs of the species' populations. [ABSTRACT FROM AUTHOR]

Caching behavior consists on the relocation or storage of food to protect it from competitors, to delay food spoilage, or to exploit it during times of scarcity. While this behavior has been widely described for some medium and large-sized felids, only a few cases documented caching behavior in small felids. Here, we provide the first exhaustive description of a caching event on a European wildcat in the Cantabrian Mountains (NW Spain). The wildcat behaved like a lynx/puma, visiting a road-killed roe deer carcass at least 9 days along a 21-day period, consuming the main muscles and covering it with hair and vegetation. [ABSTRACT FROM AUTHOR]

Red foxes, European wildcats and domestic cats share cattle pastures for hunting in La Pernía Valley, northern Spain. To understand the mechanisms that allow the coexistence of these mesopredators in a habitat characterized by its anthropogenic modifications, we recorded sightings of these species in pastures in the summers of 2016, 2017, 2018 and 2019. We tested if the species preferred specific areas of pastures and if they exhibited any spatial segregation in the use of pastures. Red foxes did not show consistent preferences for any area of the pastures. European wildcats preferred pasture areas closer to streams and forest edges, whereas domestic cats preferred areas closer to buildings and paved roads whilst avoiding forest edges. All species pairs showed strong spatial segregation with less than 7% overlap. We hypothesize that spatial segregation is the mechanism used by European wildcats and domestic cats to avoid dangerous interactions with other predators and which characterizes their preference of specific areas on pastures, using areas near places that may protect them from other predators. Ultimately, the influence of fox presence (and probably that of other larger potential predators) on the use of pastures by European wildcats and domestic cats is decreasing the number of interactions between them and may help to prevent hybridization in this area. [ABSTRACT FROM AUTHOR]

• A high prevalence of Thelazia callipaeda eyeworm infection exists in endemic areas. • Our data indicate the spread of thelaziosis in Spain and suggest underdiagnosed cases. • Animal and human thelaziosis should be included in the ocular differential diagnosis. • The role of health education under the umbrella of One Health is essential. Thelazia callipaeda (Spirurida, Thelaziidae) causes ocular infection in carnivorous animals and humans. While growing numbers of companion dogs and sometimes cats are being diagnosed with thelaziosis, little is known about its real spread. As it has been also diagnosed in wild animals and human beings, it is considered a potential emerging zoonotic disease. This study provides information about the spread of the parasite in dogs in Spain since its initial description in 2010 until 2018. The first detection of T. callipaeda in the Principality of Andorra in 2017 is also reported. Two different studies were conducted: a) a survey in which clinical cases from veterinary practices were collected and b) a prevalence study in two endemic areas in western and central Spain (Site 1 La Vera region, Cáceres, and Site 2 El Escorial municipality, Madrid). In total, 1114 cases of thelaziosis were detected in 121 municipalities of Spain and 6 municipalities of Andorra. In 92 out these 127 municipalities, reports were of autochthonous cases. Six hundred twenty-three out of 1114 presented data collection sheet and were included in the statistical analysis: 510 cases identified by veterinarians in Spain and Andorra in Study 1, and 113 cases detected among the 234 dogs (48.3 %) examined in the prevalence study (Study 2). Prevalences were 61.3 % (84/137) for Cáceres and 29.9 % (29/97) for Madrid, being Site 1 significantly more risky (P < 0.0001, odds ratio: 3.72, CI: 2.14–4.47 %) compared to Site 2. Our study updates data for canine thelaziosis reported in the last decade in Spain and Andorra. Results highlight the urgent need for prevention strategies to control the spread of this potential zoonotic disease. [ABSTRACT FROM AUTHOR]

Lethal interactions between members of the carnivore guild are well represented in literature. In the Iberian lynx, interspecific killing (without prey consumption) of some mesocarnivores, such as the Egyptian mongoose, genet, and red fox, has been reported. Although vaguely documented, evidence suggests feral cats fall victim to interactions with this apex predator. Here, we describe the first documented case of interspecific killing and partial consumption of a feral cat by an adult male Iberian lynx reintroduced in Southwestern Spain. Ulterior analyses demonstrated that the victim was viremic to feline leukemia virus. To prevent the dissemination of the virus and a potential outbreak in the Iberian lynx population, control measures, including the clinical evaluation of the male Iberian lynx, and intensive monitoring were implemented in order to detect intraspecific interactions. After 3 weeks, the lynx was evaluated, presented good condition and resulted negative to both ELISA and RT-PCR. Thanks to the long-term monitoring, this case could be detected and measures to prevent an outbreak could be implemented. [ABSTRACT FROM AUTHOR]