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An electrochemical immunosensor involving magnetic beads and disposable carbon screen-printed electrodes (SPEs) for zearalenone (Zea) sensing has been developed and evaluated using a certified reference material (CRM) and selected baby food samples. After the immunochemical reaction, the modified paramagnetic beads were confined by a magnet on surface of SPE platforms where electrochemical detection is simply achieved through the suitable substrate and mediator for the HRP enzyme. A remarkable detection limit of 0.007 microg L(-1) and excellent accuracy with recovery rate of 101-111% showed the proposed system to be a very suitable screening tool for the analysis of zearalenone in baby food samples. A new simple, fast and reliable strategy involving the sequential performing of calibration and analysis of target mycotoxin using just one disposable SPE each is additionally proposed. Excellent analytical performance in terms of accuracy and precision were again obtained with a remarkable low systematic error (less than 4%) and excellent reproducibility (RSD=6%). This strategy enhanced the analytical merits of immunosensor approach towards truly analytical disposable tools for food-safety scene., ((c) 2009 Elsevier B.V. All rights reserved.)

A 31 years old woman with a terminal phase dilated cardiomyopathy and a ventricular ejection fraction of 0.12 was admitted to our hospital to be included in the cardiac transplantation program. When entering the hospital her condition worsened and she suffered an electromechanical dissociation that needed cardiopulmonary resuscitation. Few hours later, energetic medical treatment could not improve her haemodynamic situation. Having no adequate organ available for emergency transplantation, a total orthotopic prosthesis (Jarvik-7-70) implantation was decided. Anesthesia was maintained with fentanyl (50 micrograms/kg), droperidol (0.23 mg/kg) and diazepam (0.25 mg/kg). There were no incidents during or after extracorporeal circulation bypass; despite the use of furosemide (100 mg) and mannitol (1 g/kg) diuresis was less than 40 ml/h. After by-pass, pulmonary hypertension was observed and treated with sodium nitroprusside (1 microgram/kg/min) and isoproterenol (5 micrograms/kg/min), as well as increasing the right ventricle working pressure of the device from 40 to 70 mmHg, obtaining haemodynamic stabilization and good tissular perfusion. After operation, the patient arrived to the Recovery Unit in a steady situation, with a cardiac index of 2.87 l/min/m2 and a diuresis of 150 ml/h. Some other aspects of the anaesthetic management are also discussed in this paper.