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Identifying evidence for hunter-gatherers in the palaeoenvironmental record is far from simple. Despite decades of research, few studies have demonstrated unambiguous palynological evidence of hunter-gatherers. This paper presents the results of high-resolution palaeoecological analyses of a peat sequence located within the vicinity of the pre-historic Yup’ik village of Nunalleq in southwestern Alaska. The aim of this research was to examine whether there are any discernible palaeoenvironmental impacts associated with the 15th–17th century occupation of the site. Presuming an ephemeral character to any palaeoecological signal, this study selected a sampling location approximately 30 m east of the limit of archaeological remains. Pollen, non-pollen palynomorphs and microscopic charcoal analysis were then used to generate a highly resolved (contiguous 1 cm) environmental history for the site. The results are striking and indicate that the activities of prehistoric Yup’ik hunter-gatherers at Nunalleq did leave a clear material trace in the palaeoenvironmental record. Through the application of high-resolution Pb210 and C14 dating and Bayesian modelling, these impacts were found to be concurrent with the occupation of the archaeological site. These findings suggest that not only can circumpolar hunter-gatherers leave a material palaeoenvironmental trace but that these traces may be used to accurately date such site activity in lieu of excavation. [ABSTRACT FROM AUTHOR]