1. Crotalphine, a novel potent analgesic peptide from the venom of the South American rattlesnake Crotalus durissus terrificus.
- Author
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Konno K, Picolo G, Gutierrez VP, Brigatte P, Zambelli VO, Camargo AC, and Cury Y
- Subjects
- Amino Acid Sequence, Analgesics isolation & purification, Animals, Crotoxin chemistry, Dose-Response Relationship, Drug, Male, Molecular Sequence Data, Pain Measurement drug effects, Peptides isolation & purification, Rats, Rats, Inbred BB, South America, Spectrometry, Mass, Electrospray Ionization, Analgesics chemistry, Analgesics pharmacology, Crotalid Venoms chemistry, Crotalus, Peptides chemistry, Peptides pharmacology
- Abstract
We have shown that the venom of the South American rattlesnake Crotalus durissus terrificus induces a long-lasting antinociceptive effect mediated by activation of kappa- and delta-opioid receptors. Despite being mediated by opioid receptors, prolonged treatment with the crotalid venom does not cause the development of peripheral tolerance or abstinence symptoms upon withdrawal. In the present study, we have isolated and chemically characterized a novel and potent antinociceptive peptide responsible for the oral opioid activity of this crotalid venom. The amino acid sequence of this peptide, designated crotalphine, was determined by mass spectrometry and corroborated by solid-phase synthesis to be
- Published
- 2008
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