1. Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA).
- Author
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Esteves PA, Dellagostin OA, Pinto LS, Silva AD, Spilki FR, Ciacci-Zanella JR, Hübner SO, Puentes R, Maisonnave J, Franco AC, Rijsewijk FA, Batista HB, Teixeira TF, Dezen D, Oliveira AP, David C, Arns CW, and Roehe PM
- Subjects
- Animals, Cattle, Cattle Diseases diagnosis, Herpesviridae Infections diagnosis, Herpesviridae Infections virology, Herpesvirus 1, Bovine genetics, Herpesvirus 1, Bovine isolation & purification, Herpesvirus 5, Bovine genetics, Herpesvirus 5, Bovine isolation & purification, Phylogeny, South America epidemiology, Viral Envelope Proteins genetics, Cattle Diseases virology, Herpesviridae Infections veterinary, Herpesvirus 1, Bovine classification, Herpesvirus 5, Bovine classification, Viral Envelope Proteins chemistry
- Abstract
Different types and subtypes of bovine herpesvirus 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, in such a way that type/subtype differentiation has become an essential tool for understanding the pathogenesis and epidemiology of BoHV infections. In search for a genomic region that would allow a clear distinction between BoHV-1 and BoHV-5, the carboxy-terminal portion of glycoprotein C (gC), corresponding to residues 321-450 (BoHV-1) and 301-429 (BoHV-5) of 23 South American (SA) isolates (Brazil mostly) was amplified and sequenced. The nucleotide sequence alignments revealed levels of genomic similarity ranging from 98.7 to 99.8% among BoHV-1 isolates, 88.3 to 92% between BoHV-1/BoHV-5 and 96 to 99.7% among BoHV-5 isolates. At the amino acid level, sequence similarity varied ranging from 97.5 to 99.5% among BoHV-1, 77.5 to 84.4% between BoHV-1/BoHV-5 and 92.1 to 99.5% (BoHV-5/BoHV-5). The isolates could be clearly separated into BoHV-1.1, BoHV-1.2 and BoHV-5 after phylogenetic analysis. The results suggest that the phylogenetic analysis performed here can be used as a potential molecular epidemiological tool for herpesviruses.
- Published
- 2008
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