1. Molecular characterization of 'Candidatus Rickettsia vini' in Ixodes arboricola from the Czech Republic and Slovakia.
- Author
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Novakova M, Bulkova A, Costa FB, Kristin A, Krist M, Krause F, Liznarova E, Labruna MB, and Literak I
- Subjects
- Animals, Bacterial Proteins genetics, Bacterial Typing Techniques veterinary, Base Sequence, Czech Republic epidemiology, Female, Male, Molecular Sequence Data, Multilocus Sequence Typing veterinary, Nymph, Passeriformes, Phylogeny, Rickettsia classification, Rickettsia genetics, Rickettsia Infections epidemiology, Rickettsia Infections microbiology, Slovakia epidemiology, Tick Infestations epidemiology, Trees, Ixodes microbiology, Rickettsia isolation & purification, Rickettsia Infections veterinary, Tick Infestations veterinary
- Abstract
The aim of this study was to analyze the prevalence of rickettsiae in the tree-hole tick Ixodes arboricola in the Czech Republic and Slovakia. During May to September of 2009 and 2013, bird boxes belonging to three different areas were screened for ticks. In total, 454 nestlings and 109 nests of 10 hole-breeding bird species were examined. Ticks were found on Ficedula albicollis, Parus major, Cyanistes caeruleus and Sitta europaea and/or in their nests. In total, 166 ticks (17 nymphs, 10 males and 139 females) were found at 3 areas (arithmetic mean±standard error: 55.3±45.9). All ticks were tested for the presence of Rickettsia species by polymerase chain reaction targeting the rickettsial genes gltA, ompA, ompB and htrA and amplicon sequencing. All individuals except 3 nymphs were infected with 'Candidatus Rickettsia vini'. Multilocus sequence typing showed closest proximity to Rickettsia japonica and Rickettsia heilongjiangensis cluster. The presence of 'Ca. R. vini' is reported for the first time in Slovakia., (Copyright © 2015 Elsevier GmbH. All rights reserved.)
- Published
- 2015
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