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This study aimed to investigate the effect of Lactiplantibacillus plantarum SCS4 in alleviating oxidative stress in the pancreatic tissue of hyperglycemic mice. A total of 90 six‐week‐old specific pathogen‐free male Kunming mice were randomly divided into six groups [normal group (NG), blank control group (MG), phosphate‐buffered saline (PBS) control group (CG), SCS4 first control group (DT1), SCS4 second control group (DT2), and SCS4 third control group (DT3)]. Except the NG group, in the other five groups, streptozotocin (STZ) was intraperitoneally injected for five consecutive days to establish a hyperglycemia model; the concentration of STZ was 50 mg/kg (bw). After successful modeling, DT1, DT2, and DT3 mice were administered 0.2 ml of L. plantarum SCS4 bacterial solution (1010 colony forming unit/ml), the cellular content of L. plantarum SCS4, and the inactivated cellular content of L. plantarum SCS4, respectively. Furthermore, 0.2 ml of PBS was given to mice in the CG group for control. The levels of insulin (INS), reactive oxygen species (ROS), malondialdehyde (MDA), and antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured by enzyme‐linked immunosorbent assay, and the morphology of the pancreas was observed. The results showed that after 10 weeks of gavage treatment, the level of INS in the DT3 group significantly increased to 6.36 mIU/L compared with that in the MG group (p <.05). Meanwhile, the levels of ROS and MDA of DT3 returned to normal levels of 291.07 IU/ml and 4.29 mnol/L, respectively. The activities of various antioxidant enzymes increased. The levels of SOD, CAT, and GPx in DT3 were the closest to the levels in NG. In addition, the pathological sections showed that the degree of pancreatic tissue lesions was relatively more severe in the MG group than in the NG group. The degree of pancreatic tissue lesions was relatively less severe in the DT2 group than in the MG group, and no significant lesion was seen in the DT3 group. Our results indicated that the inactivated bacterial content of L. planetarium SCS4 was more effective in improving oxidative stress in the pancreas of hyperglycemic mice. Practical applications: L. plantarum SCS4 was separated from fermented sausage in Sichuan. This study indicated that inactivated bacterial content of L. planetarium SCS4 was more effective in improving oxidative stress in the pancreas of hyperglycemic mice. The results suggested that lactic acid bacteria from traditional foods with ability of improving oxidative damage, which can be applied in food nutrition and related fields to make people with good dietary habits and prevent the occurrence of chronic diseases such as type II diabetes effectively. [ABSTRACT FROM AUTHOR]

The Chipu Mississippi Valley-type (MVT) deposit is located on the southwest (SW) margin of the Sichuan Basin. Occurrence of plentiful organic matter (bitumen) at this deposit and abundant hydrocarbon reservoirs in the SW Sichuan Basin implies a link between lead–zinc mineralization and hydrocarbon systems in this area. The high δ34S values of most metal sulphides from the different ore stages suggest that H2S-bearing gases and/or thermochemical sulphate reduction (TSR) by organic matter could have been the source of reduced sulphur involved in ore formation. Sulphides with small positive to negative δ34S values can be attributed to organically bound sulphur at the Chipu deposit. Carbon and oxygen isotopic compositions from sparry carbonates suggest mixing of organic carbon with seawater-derived carbon in the mineralization process. From the early to the later ore stages, δ13CPDBvalues of ore-hosting carbonates are increasingly more negative, which indicates strengthening of the TSR role during mineralization. Hydrogen and oxygen isotopes in fluid inclusions in the quartz gangue indicate the provenance of the ore-forming fluids in the hydrocarbons. Moreover, some extremely low hydrogen isotope values suggest the addition of hydrogen from the same source. The low H/C ratios and high non-hydrocarbon component of the bitumen (Zhanget al. 2010) also suggest that the organic matter may have been involved in TSR and subjected to a strong oxidation by ore-bearing fluids. This study attempts to explain the lead–zinc mineralization process and the role of organic matter in it. As there is a demonstrable relationship between the evolution of the hydrocarbons and regional lead–zinc mineralization along the SW edge of the Sichuan Basin, we propose a possible model in which the MVT mineralization coincided with the degradation of hydrocarbon reservoirs due to the large-scale migration of basinal fluids, most likely driven by the late Indosinian orogeny in response to the closure of the Palaeo-Tethys Ocean. [ABSTRACT FROM AUTHOR]