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This paper studied the Anaerobic-Anoxic-Oxic (A2/O) process of a city sewage treatment plant in Sichuan Province, China. We established a mathematical model of sewage treatment plant. The yield coefficient of heterotrophic bacteria (YH) in A2/O process was 0.65; maximum specific growth rate (uH) of 8.6 d-1; attenuation coefficient (bH) of 3.5 d-1. Compared with the recommended values reported in the literature, the results showed that the yield coefficient of heterotrophic bacteria measured by this method was consistent with the recommended value and the maximum specific growth rate and attenuation coefficient were higher than the recommended value. Different water qualities of different processes and heterotrophic bacteria in the activity are not the same. So the determination of the results will be different. Using the Electrolytic Respirometer developed by Bioscience Incorporation (BI-2000), the microbial oxygen uptake rate (OUR) curve is more accurate, the sampling frequency is higher, the sampling point is more, the judgment of the logarithmic period is more accurate, the test result is more reliable and the sewage treatment plant of the upgrade and simulation to optimize the operation to provide a theoretical basis. [ABSTRACT FROM AUTHOR]