1. [The first break-through of the genotype 2.3.2 of high-virulence influenza A virus A/HSN1, which is new for Russia, in the Far East].
- Author
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L'vov DK, Zhchelkanov MIu, Vlasov NA, Prilipov AG, Deriabin PG, Fediakina IT, Galkina IV, Zaberezhnyĭ AD, Liapina OV, Shliapnikova OV, Kireev DE, Fesenko EE, Kalmykov MV, Vitkova ON, Morozova TN, Proshina ES, Grebennikova TV, Akanina DS, Samokhvalov EI, Al'khovskiĭ SV, Volkov VA, Semenov VI, Gaponov VV, Shmakov NI, Kushnir AT, Kazarian AS, Starikov NS, Petrenko MS, Slavskiĭ AA, Litvin KE, Shcherbakova LO, Frolov AV, Manin TB, Umanets OA, Bandeev VV, Khvan AM, Dunaev VG, Cheledina TP, Abgarian SR, Mikhaĭlovich VM, Zasedatelev AS, Liubchenko EN, Fliagin VN, Tikhonova IF, Maslov DV, Anan'ev VIu, Baranov NI, Gorelikov VN, Iakovlev SS, Aliper TI, Nepoklonov EA, and Suarez D
- Subjects
- Animal Migration, Animals, Antiviral Agents pharmacology, Birds virology, Chickens virology, Genome, Viral, Genotype, Influenza A Virus, H5N1 Subtype drug effects, Influenza A Virus, H5N1 Subtype isolation & purification, Molecular Sequence Data, Oseltamivir pharmacology, Phylogeny, Rimantadine pharmacology, Siberia epidemiology, Disease Outbreaks, Influenza A Virus, H5N1 Subtype classification, Influenza A Virus, H5N1 Subtype genetics, Influenza in Birds epidemiology, Influenza in Birds virology
- Abstract
The epizootic etiologically associated with highly pathogenic avian influenza H5N1 genotype 2.3.2 that is new for Russia among wild and domestic birds in the south of the Primorye Territory during spring migration in April 2008 has been decoded. About 25% of the wild birds of a water complex, which include European teals (Anas crecca), mallard ducks (Anas platyrhynchos), great-crested grebes (Podiceps cristatus), are involved in viral circulation in the area of the Suifun-Khankai plain. Chicken embryos and the cell lines MDCK, SPEV, BHK-21, SW-13 were used to isolate 3 strains from recently deceased hens (A/chicken/Primorje/1/08, A/chicken/Primorje/11/08, and A/chicken/Primorje/12/08) and one strain from a European teal (A/Anas crecca/Primorje/8/08). The strains were deposited in the State Collection of Viruses of the Russian Federation, D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences. The nucleotide sequences of the full-sized genomes of A/chicken/Primorje/1/08 and A/Anas crecca/Primorje/8/08 were sent to the International databank GenBank. The strains from domestic and wild birds were shown to be identical. The isolated strains are most close to the strains Alchicken/Viet Nam/10/05, A/chicken/Guangdong/178/04, and A/duck/Viet Nam/12/05. Molecular genetic analysis has indicated that the strains isolated are susceptible to rimantadine and ozeltamivir and less adapted to mammalian cells (particularly, they contain E627 in RV2, which agrees with the biological properties of these strains in vitro). Penetration of the newly isolated virus into the Far East ecosystem provides in the foreseeable future a way for infecting the birds wintering in America and Australia in the nesting places, with further carriage of viral populations there in the period of autumn migrations.
- Published
- 2008