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1. [DNA from various tissues of the Far Eastern larch and its applicability for RAPD assay].

Author

Sazonova IIu, Kozyrenko MM, Artiukova EV, Reunova GD, and Zhuravlev IuN

Subjects
Asia, Eastern, Random Amplified Polymorphic DNA Technique, Siberia, DNA, Plant analysis, Plant Structures chemistry, Trees chemistry
Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Published
2001

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