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The integration of multi-source and diverse spatio-temporal travel data provides a comprehensive insight into urban mobility. Using data from Shenzhen's public transportation system, this study presents an analytical framework based on multiplex networks to examine variations in multi-mode public transportation usage (metro, bus, taxi, and shared bike) and their correlation with the built environment. This framework encompasses the analysis of network topological characteristics, centrality, and communities. The examination of network topological characteristics reveals that the multiplex transportation network exhibits high global accessibility and local connectivity. Network centrality analysis, focusing on weighted outdegree centrality, captures the patterns of public transportation ridership. Centrality modeling, employing the light gradient boosting machine, demonstrates a nonlinear relationship between ridership and the built environment. Factors including population density, residential land use percentage, entertainment service density, restaurant density, and metro station density consistently exhibit positive correlations with ridership across different times of the day. The community structure analysis, using consensus community detection, indicates that distinct urban areas exhibit clustering behavior based on public transportation demand patterns, forming distinct communities that closely align with the functional zoning of urban planning. These findings could provide valuable insights for the strategic planning of transportation services and the built environment. • Multi-source public transportation data are integrated to investigate urban mobility. • A multiplex network-based framework is developed to explore public transportation use. • Topological structure analysis reveals high global accessibility and local connectivity. • Centrality analysis reveals nonlinear correlations between ridership and the built environment. • Communities within multiplex networks closely align with the urban functional zoning. [ABSTRACT FROM AUTHOR]